December 2009 - galaxy-dev - lists.galaxyproject.org

[hg] galaxy 3135: Adding back 'wiggle to interval' converter
by Greg Von Kuster 02 Dec '09

02 Dec '09

details: http://www.bx.psu.edu/hg/galaxy/rev/eb317734e391 changeset: 3135:eb317734e391 user: guru date: Tue Dec 01 11:49:07 2009 -0500 description: Adding back 'wiggle to interval' converter diffstat: datatypes_conf.xml.sample | 1 + lib/galaxy/datatypes/converters/wiggle_to_simple_converter.py | 44 +++++++ lib/galaxy/datatypes/converters/wiggle_to_simple_converter.xml | 11 + test-data/3.wig | 37 ++++++ test-data/3_wig.bed | 26 ++++ tool_conf.xml.main | 1 + tool_conf.xml.sample | 1 + tools/filters/wiggle_to_simple.py | 43 +++++++ tools/filters/wiggle_to_simple.xml | 88 ++++++++++++++ 9 files changed, 252 insertions(+), 0 deletions(-) diffs (306 lines): diff -r 6e742ffb16a5 -r eb317734e391 datatypes_conf.xml.sample --- a/datatypes_conf.xml.sample Tue Dec 01 11:07:20 2009 -0500 +++ b/datatypes_conf.xml.sample Tue Dec 01 11:49:07 2009 -0500 @@ -58,6 +58,7 @@ <datatype extension="txtseq.zip" type="galaxy.datatypes.data:Txtseq" mimetype="application/zip" display_in_upload="true"/> <datatype extension="wig" type="galaxy.datatypes.interval:Wiggle" display_in_upload="true"> <converter file="wiggle_to_array_tree_converter.xml" target_datatype="array_tree"/> + <converter file="wiggle_to_simple_converter.xml" target_datatype="interval"/> </datatype> <datatype extension="array_tree" type="galaxy.datatypes.data:Data" /> <datatype extension="interval_index" type="galaxy.datatypes.data:Data" /> diff -r 6e742ffb16a5 -r eb317734e391 lib/galaxy/datatypes/converters/wiggle_to_simple_converter.py --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/lib/galaxy/datatypes/converters/wiggle_to_simple_converter.py Tue Dec 01 11:49:07 2009 -0500 @@ -0,0 +1,44 @@ +#!/usr/bin/env python +#code is same as ~/tools/stats/wiggle_to_simple.py + +""" +Read a wiggle track and print out a series of lines containing +"chrom position score". Ignores track lines, handles bed, variableStep +and fixedStep wiggle lines. +""" +import sys +from galaxy import eggs +import pkg_resources; pkg_resources.require( "bx-python" ) +import bx.wiggle +from galaxy.tools.exception_handling import * + +def stop_err( msg ): + sys.stderr.write( msg ) + sys.exit() + +def main(): + if len( sys.argv ) > 1: + in_file = open( sys.argv[1] ) + else: + in_file = open( sys.stdin ) + + if len( sys.argv ) > 2: + out_file = open( sys.argv[2], "w" ) + else: + out_file = sys.stdout + + try: + for fields in bx.wiggle.IntervalReader( UCSCOutWrapper( in_file ) ): + out_file.write( "%s\n" % "\t".join( map( str, fields ) ) ) + except UCSCLimitException: + # Wiggle data was truncated, at the very least need to warn the user. + print 'Encountered message from UCSC: "Reached output limit of 100000 data values", so be aware your data was truncated.' + except ValueError, e: + in_file.close() + out_file.close() + stop_err( str( e ) ) + + in_file.close() + out_file.close() + +if __name__ == "__main__": main() diff -r 6e742ffb16a5 -r eb317734e391 lib/galaxy/datatypes/converters/wiggle_to_simple_converter.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/lib/galaxy/datatypes/converters/wiggle_to_simple_converter.xml Tue Dec 01 11:49:07 2009 -0500 @@ -0,0 +1,11 @@ +<tool id="CONVERTER_wiggle_to_interval_0" name="Wiggle to Interval"> +  +  + <command interpreter="python">wiggle_to_simple_converter.py $input $out_file1 </command> + <inputs> + <param format="wig" name="input" type="data" label="Convert"/> + </inputs> + <outputs> + <data format="interval" name="out_file1" /> + </outputs> +</tool> diff -r 6e742ffb16a5 -r eb317734e391 test-data/3.wig --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/3.wig Tue Dec 01 11:49:07 2009 -0500 @@ -0,0 +1,37 @@ +track type=wiggle_0 name="LaminB1 (Tig3)" description="NKI LaminB1 DamID Map (log2-ratio scores, Tig3 cells)" +# output date: 2009-07-22 15:55:44 UTC +# chrom specified: chr20 +# position specified: 1-62435964 +# data values >= 4 +# This data has been compressed with a minor loss in resolution. +# (Worst case: 0.0448672) The original source data +# (before querying and compression) is available at +# http://hgdownload.cse.ucsc.edu/downloads.html +variableStep chrom=chr20 span=60 +1953668 4.029 +9749454 4.033 +12219969 4.053 +12571904 4.548 +12663417 4.207 +15198014 4.041 +16613945 4.526 +16994767 4.08 +19456653 4.014 +37706076 4.293 +38010695 4.14 +38418792 4.133 +38595669 4.032 +40038834 4.144 +50693133 4.206 +51294426 4 +52890600 4.12 +57414708 4.026 +57623989 4.031 +58977191 5.678 +59059925 4.325 +variableStep chrom=chr12 span=60 +1953668 -4.029 +9749454 -4.033 +12219969 -4.053 +12571904 -4.548 +12663417 -4.207 diff -r 6e742ffb16a5 -r eb317734e391 test-data/3_wig.bed --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/3_wig.bed Tue Dec 01 11:49:07 2009 -0500 @@ -0,0 +1,26 @@ +chr20 1953667 1953727 + 4.029 +chr20 9749453 9749513 + 4.033 +chr20 12219968 12220028 + 4.053 +chr20 12571903 12571963 + 4.548 +chr20 12663416 12663476 + 4.207 +chr20 15198013 15198073 + 4.041 +chr20 16613944 16614004 + 4.526 +chr20 16994766 16994826 + 4.08 +chr20 19456652 19456712 + 4.014 +chr20 37706075 37706135 + 4.293 +chr20 38010694 38010754 + 4.14 +chr20 38418791 38418851 + 4.133 +chr20 38595668 38595728 + 4.032 +chr20 40038833 40038893 + 4.144 +chr20 50693132 50693192 + 4.206 +chr20 51294425 51294485 + 4.0 +chr20 52890599 52890659 + 4.12 +chr20 57414707 57414767 + 4.026 +chr20 57623988 57624048 + 4.031 +chr20 58977190 58977250 + 5.678 +chr20 59059924 59059984 + 4.325 +chr12 1953667 1953727 + -4.029 +chr12 9749453 9749513 + -4.033 +chr12 12219968 12220028 + -4.053 +chr12 12571903 12571963 + -4.548 +chr12 12663416 12663476 + -4.207 diff -r 6e742ffb16a5 -r eb317734e391 tool_conf.xml.main --- a/tool_conf.xml.main Tue Dec 01 11:07:20 2009 -0500 +++ b/tool_conf.xml.main Tue Dec 01 11:49:07 2009 -0500 @@ -47,6 +47,7 @@ <tool file="maf/maf_to_fasta.xml" /> <tool file="fasta_tools/tabular_to_fasta.xml" /> <tool file="fastx_toolkit/fastq_to_fasta.xml" /> + <tool file="filters/wiggle_to_simple.xml" /> </section> <section name="FASTA manipulation" id="fasta_manipulation"> <tool file="fasta_tools/fasta_compute_length.xml" /> diff -r 6e742ffb16a5 -r eb317734e391 tool_conf.xml.sample --- a/tool_conf.xml.sample Tue Dec 01 11:07:20 2009 -0500 +++ b/tool_conf.xml.sample Tue Dec 01 11:49:07 2009 -0500 @@ -71,6 +71,7 @@ <tool file="maf/maf_to_fasta.xml" /> <tool file="fasta_tools/tabular_to_fasta.xml" /> <tool file="fastx_toolkit/fastq_to_fasta.xml" /> + <tool file="filters/wiggle_to_simple.xml" /> </section> <section name="Extract Features" id="features"> <tool file="filters/ucsc_gene_bed_to_exon_bed.xml" /> diff -r 6e742ffb16a5 -r eb317734e391 tools/filters/wiggle_to_simple.py --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/filters/wiggle_to_simple.py Tue Dec 01 11:49:07 2009 -0500 @@ -0,0 +1,43 @@ +#!/usr/bin/env python + +""" +Read a wiggle track and print out a series of lines containing +"chrom position score". Ignores track lines, handles bed, variableStep +and fixedStep wiggle lines. +""" +import sys +from galaxy import eggs +import pkg_resources; pkg_resources.require( "bx-python" ) +import bx.wiggle +from galaxy.tools.exception_handling import * + +def stop_err( msg ): + sys.stderr.write( msg ) + sys.exit() + +def main(): + if len( sys.argv ) > 1: + in_file = open( sys.argv[1] ) + else: + in_file = open( sys.stdin ) + + if len( sys.argv ) > 2: + out_file = open( sys.argv[2], "w" ) + else: + out_file = sys.stdout + + try: + for fields in bx.wiggle.IntervalReader( UCSCOutWrapper( in_file ) ): + out_file.write( "%s\n" % "\t".join( map( str, fields ) ) ) + except UCSCLimitException: + # Wiggle data was truncated, at the very least need to warn the user. + print 'Encountered message from UCSC: "Reached output limit of 100000 data values", so be aware your data was truncated.' + except ValueError, e: + in_file.close() + out_file.close() + stop_err( str( e ) ) + + in_file.close() + out_file.close() + +if __name__ == "__main__": main() diff -r 6e742ffb16a5 -r eb317734e391 tools/filters/wiggle_to_simple.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/filters/wiggle_to_simple.xml Tue Dec 01 11:49:07 2009 -0500 @@ -0,0 +1,88 @@ +<tool id="wiggle2simple1" name="Wiggle-to-Interval"> + <description>converter</description> + <command interpreter="python">wiggle_to_simple.py $input $out_file1 </command> + <inputs> + <param format="wig" name="input" type="data" label="Convert"/> + </inputs> + <outputs> + <data format="interval" name="out_file1" /> + </outputs> + <tests> + <test> + <param name="input" value="2.wig" /> + <output name="out_file1" file="2.interval"/> + </test> + <test> + <param name="input" value="3.wig" /> + <output name="out_file1" file="3_wig.bed"/> + </test> + </tests> + <help> +**Syntax** + +This tool converts wiggle data into interval type. + +- **Wiggle format**: The .wig format is line-oriented. Wiggle data is preceded by a UCSC track definition line. Following the track definition line is the track data, which can be entered in three different formats described below. + + - **BED format** with no declaration line and four columns of data:: + + chromA chromStartA chromEndA dataValueA + chromB chromStartB chromEndB dataValueB + + - **variableStep** two column data; started by a declaration line and followed with chromosome positions and data values:: + + variableStep chrom=chrN [span=windowSize] + chromStartA dataValueA + chromStartB dataValueB + + - **fixedStep** single column data; started by a declaration line and followed with data values:: + + fixedStep chrom=chrN start=position step=stepInterval [span=windowSize] + dataValue1 + dataValue2 + +----- + +**Example** + +- input wiggle format file:: + + #track type=wiggle_0 name="Bed Format" description="BED format" + chr19 59302000 59302300 -1.0 + chr19 59302300 59302600 -0.75 + chr19 59302600 59302900 -0.50 + chr19 59302900 59303200 -0.25 + chr19 59303200 59303500 0.0 + #track type=wiggle_0 name="variableStep" description="variableStep format" + variableStep chrom=chr19 span=150 + 59304701 10.0 + 59304901 12.5 + 59305401 15.0 + 59305601 17.5 + #track type=wiggle_0 name="fixedStep" description="fixed step" visibility=full + fixedStep chrom=chr19 start=59307401 step=300 span=200 + 1000 + 900 + 800 + 700 + 600 + +- convert the above file to interval file:: + + chr19 59302000 59302300 + -1.0 + chr19 59302300 59302600 + -0.75 + chr19 59302600 59302900 + -0.5 + chr19 59302900 59303200 + -0.25 + chr19 59303200 59303500 + 0.0 + chr19 59304701 59304851 + 10.0 + chr19 59304901 59305051 + 12.5 + chr19 59305401 59305551 + 15.0 + chr19 59305601 59305751 + 17.5 + chr19 59307701 59307901 + 1000.0 + chr19 59308001 59308201 + 900.0 + chr19 59308301 59308501 + 800.0 + chr19 59308601 59308801 + 700.0 + chr19 59308901 59309101 + 600.0 + +</help> +</tool>

1 0

[hg] galaxy 3131: Added ability for a user to add additional sto...
by Greg Von Kuster 02 Dec '09

02 Dec '09

details: http://www.bx.psu.edu/hg/galaxy/rev/1d93eb88c93f changeset: 3131:1d93eb88c93f user: Enis Afgan <afgane(a)gmail.com> date: Mon Nov 30 16:36:38 2009 -0500 description: Added ability for a user to add additional storage volume(s) to a running EC2 instance. In this context, a new EBS volume is created, attached to a running instance, and then added to remote zpool. When an instance is shut down and then started back up, all of the associated storage volumes are automatically attached to it and the zpool is imported. diffstat: lib/galaxy/cloud/__init__.py | 100 +++++++++- lib/galaxy/cloud/providers/ec2.py | 300 +++++++++++++++++++++++++++----- lib/galaxy/cloud/providers/eucalyptus.py | 20 +- lib/galaxy/web/controllers/cloud.py | 93 +++++++++- templates/cloud/configure_cloud.mako | 19 +- 5 files changed, 445 insertions(+), 87 deletions(-) diffs (922 lines): diff -r f4654abcec1e -r 1d93eb88c93f lib/galaxy/cloud/__init__.py --- a/lib/galaxy/cloud/__init__.py Fri Nov 20 11:03:41 2009 -0500 +++ b/lib/galaxy/cloud/__init__.py Mon Nov 30 16:36:38 2009 -0500 @@ -29,6 +29,8 @@ SUBMITTED = "submitted", SHUTTING_DOWN_UCI = "shutting-downUCI", SHUTTING_DOWN = "shutting-down", + ADD_STORAGE_UCI = "add-storageUCI", + ADD_STORAGE = "add-storage", AVAILABLE = "available", RUNNING = "running", PENDING = "pending", @@ -40,6 +42,7 @@ TERMINATED = "terminated", SUBMITTED = "submitted", RUNNING = "running", + ADDING = "adding-storage", PENDING = "pending", SHUTTING_DOWN = "shutting-down", ERROR = "error" @@ -48,6 +51,7 @@ store_status = Bunch( WAITING = "waiting", IN_USE = "in-use", + ADDING = "adding", CREATING = "creating", DELETED = 'deleted', ERROR = "error" @@ -161,7 +165,8 @@ model.UCI.table.c.state==uci_states.SUBMITTED_UCI, model.UCI.table.c.state==uci_states.SHUTTING_DOWN_UCI, model.UCI.table.c.state==uci_states.DELETING_UCI, - model.UCI.table.c.state==uci_states.SNAPSHOT_UCI ) ) \ + model.UCI.table.c.state==uci_states.SNAPSHOT_UCI, + model.UCI.table.c.state==uci_states.ADD_STORAGE_UCI ) ) \ .all(): uci_wrapper = UCIwrapper( r, self.app ) new_requests.append( uci_wrapper ) @@ -195,6 +200,12 @@ self.uci_id = uci.id self.app = app self.sa_session = self.app.model.context + base_directory = os.path.join( self.app.config.job_working_directory, "cloud" ) + self.working_directory = os.path.join( base_directory, str( self.uci_id ) ) +# log.debug( "Cloud controller working directory for UCI DB ID '%s': '%s'" % ( self.uci_id, self.working_directory ) ) + if not os.path.exists( base_directory ): + os.mkdir( base_directory ) + # --------- Setter methods ----------------- @@ -364,11 +375,18 @@ self.sa_session.add( uci ) self.sa_session.flush() - def set_store_device( self, store_id, device ): +# def set_store_device( self, store_id, device ): +# uci = self.sa_session.query( model.UCI ).get( self.uci_id ) +# self.sa_session.refresh( uci ) +# uci.store[store_id].device = device +# uci.store[store_id].flush() + + def set_uci_total_size( self, total_size ): uci = self.sa_session.query( model.UCI ).get( self.uci_id ) self.sa_session.refresh( uci ) - uci.store[store_id].device = device - uci.store[store_id].flush() + uci.total_size = total_size + self.sa_session.add( uci ) + self.sa_session.flush() def set_store_error( self, error, store_index=None, store_id=None ): if store_index != None: @@ -405,15 +423,24 @@ def set_store_volume_id( self, store_index, volume_id ): """ - Given store index associated with this UCI in local database, set volume ID as it is registered + Given store index as it is stored in local database, set volume ID as it is registered on the cloud provider (e.g., vol-39890501) """ - uci = self.sa_session.query( model.UCI ).get( self.uci_id ) - self.sa_session.refresh( uci ) - uci.store[store_index].volume_id = volume_id - #uci.store[store_index].flush() - self.sa_session.add( uci ) - self.sa_session.flush() + + if store_index != None: + store = self.sa_session.query( model.CloudStore ).get( store_index ) + store.volume_id = volume_id + self.sa_session.add( store ) + self.sa_session.flush() + else: + return None + +# uci = self.sa_session.query( model.UCI ).get( self.uci_id ) +# self.sa_session.refresh( uci ) +# uci.store[store_index].volume_id = volume_id +# #uci.store[store_index].flush() +# self.sa_session.add( uci ) +# self.sa_session.flush() def set_store_instance( self, vol_id, instance_id ): """ @@ -421,7 +448,29 @@ be given in following format: 'vol-78943248' """ vol = self.sa_session.query( model.CloudStore ).filter( model.CloudStore.table.c.volume_id == vol_id ).first() - vol.inst.instance_id = instance_id + inst = self.sa_session.query( model.CloudInstance ).filter_by( instance_id=instance_id ).first() + vol.inst = inst + self.sa_session.add( vol ) + self.sa_session.flush() + + def set_store_device( self, vol_id, device ): + """ + Stores instance ID that given store volume is attached to. Store volume ID should + be given in following format: 'vol-78943248' + """ + vol = self.sa_session.query( model.CloudStore ).filter( model.CloudStore.table.c.volume_id == vol_id ).first() + vol.device = str( device ) + self.sa_session.add( vol ) + self.sa_session.flush() + + def set_store_deleted( self, vol_id, status=None ): + """ + Set storage volume as deleted in local database. Optionally, set the volume status too. + """ + vol = self.sa_session.query( model.CloudStore ).filter( model.CloudStore.table.c.volume_id == vol_id ).first() + vol.deleted = True + if status != None: + vol.status = status self.sa_session.add( vol ) self.sa_session.flush() @@ -503,6 +552,12 @@ self.sa_session.refresh( uci ) return uci.instance[instance_id].state + def get_instaces_in_state( self, state ): + """ Get database objects of all instances associated with this UCI in given state. """ + return self.sa_session.query( model.CloudInstance ) \ + .filter_by( uci_id=self.uci_id, state = state ) \ + .all() + def get_instances_ids( self ): """ Returns list IDs of all instances' associated with this UCI that are not in 'terminated' or @@ -590,6 +645,11 @@ self.sa_session.refresh( uci ) return uci.store[0].availability_zone + def get_uci_total_size( self ): + uci = self.sa_session.query( model.UCI ).get( self.uci_id ) + self.sa_session.refresh( uci ) + return uci.total_size + def get_store_size( self, store_id=0 ): uci = self.sa_session.query( model.UCI ).get( self.uci_id ) self.sa_session.refresh( uci ) @@ -604,9 +664,18 @@ self.sa_session.refresh( uci ) return uci.store[store_id].volume_id + def get_all_stores_in_status( self, status ): + """ + Return database objects of all stores associated with this UCI that have their + status set to value passed as parameter. + """ + return self.sa_session.query( model.CloudStore ).filter_by( deleted=False, uci_id=self.uci_id, status=status ).all() + def get_all_stores( self ): - """ Returns all storage volumes' database objects associated with this UCI. """ - return self.sa_session.query( model.CloudStore ).filter( model.CloudStore.table.c.uci_id == self.uci_id ).all() + """ Returns all storage volumes' database objects associated with this UCI that have not been marked as 'deleted'. """ + return self.sa_session.query( model.CloudStore ) \ + .filter_by( deleted=False, uci_id=self.uci_id ) \ + .all() def get_snapshots( self, status=None ): """ Returns database objects for all snapshots associated with this UCI and in given status.""" @@ -618,6 +687,9 @@ self.sa_session.refresh( uci ) return uci + def get_uci_working_directory( self ): + return self.working_directory + def uci_launch_time_set( self ): uci = self.sa_session.query( model.UCI ).get( self.uci_id ) self.sa_session.refresh( uci ) diff -r f4654abcec1e -r 1d93eb88c93f lib/galaxy/cloud/providers/ec2.py --- a/lib/galaxy/cloud/providers/ec2.py Fri Nov 20 11:03:41 2009 -0500 +++ b/lib/galaxy/cloud/providers/ec2.py Mon Nov 30 16:36:38 2009 -0500 @@ -1,4 +1,4 @@ -import subprocess, threading, os, errno, time, datetime +import subprocess, threading, os, errno, time, datetime, stat from Queue import Queue, Empty from datetime import datetime @@ -30,6 +30,8 @@ SUBMITTED = "submitted", SHUTTING_DOWN_UCI = "shutting-downUCI", SHUTTING_DOWN = "shutting-down", + ADD_STORAGE_UCI = "add-storageUCI", + ADD_STORAGE = "add-storage", AVAILABLE = "available", RUNNING = "running", PENDING = "pending", @@ -43,6 +45,7 @@ TERMINATED = "terminated", SUBMITTED = "submitted", RUNNING = "running", + ADDING = "adding-storage", PENDING = "pending", SHUTTING_DOWN = "shutting-down", ERROR = "error" @@ -51,6 +54,7 @@ store_status = Bunch( WAITING = "waiting", IN_USE = "in-use", + ADDING = "adding", CREATING = "creating", DELETED = 'deleted', ERROR = "error" @@ -122,6 +126,9 @@ self.stop_uci( uci_wrapper ) elif uci_state==uci_states.SNAPSHOT: self.snapshot_uci( uci_wrapper ) + elif uci_state==uci_states.ADD_STORAGE: + self.add_storage_to_uci( uci_wrapper ) + #self.dummy_start_uci( uci_wrapper ) except: log.exception( "Uncaught exception executing cloud request." ) cnt += 1 @@ -241,25 +248,14 @@ log.info( "Availability zone for UCI (i.e., storage volume) was not selected, using default zone: %s" % self.zone ) uci_wrapper.set_store_availability_zone( self.zone ) - log.info( "Creating volume in zone '%s'..." % uci_wrapper.get_uci_availability_zone() ) + store = uci_wrapper.get_all_stores_in_status( store_status.ADDING )[0] # Because at UCI creation time only 1 storage volume can be created, reference it directly + + log.info( "Creating storage volume in zone '%s' of size '%s'..." % ( uci_wrapper.get_uci_availability_zone(), store.size ) ) # Because only 1 storage volume may be created at UCI config time, index of this storage volume in local Galaxy DB w.r.t # current UCI is 0, so reference it in following methods - vol = conn.create_volume( uci_wrapper.get_store_size( 0 ), uci_wrapper.get_uci_availability_zone(), snapshot=None ) - uci_wrapper.set_store_volume_id( 0, vol.id ) - - # Wait for a while to ensure volume was created -# vol_status = vol.status -# for i in range( 30 ): -# if vol_status is not "available": -# log.debug( 'Updating volume status; current status: %s' % vol_status ) -# vol_status = vol.status -# time.sleep(3) -# if i is 29: -# log.debug( "Error while creating volume '%s'; stuck in state '%s'; deleting volume." % ( vol.id, vol_status ) ) -# conn.delete_volume( vol.id ) -# uci_wrapper.change_state( uci_state='error' ) -# return - + vol = conn.create_volume( store.size, uci_wrapper.get_uci_availability_zone(), snapshot=None ) + uci_wrapper.set_store_volume_id( store.id, vol.id ) + # Retrieve created volume again to get updated status try: vl = conn.get_all_volumes( [vol.id] ) @@ -306,6 +302,7 @@ if conn.delete_volume( v.volume_id ): deletedList.append( v.volume_id ) v.deleted = True + v.status = store_status.DELETED self.sa_session.add( v ) self.sa_session.flush() count += 1 @@ -358,15 +355,180 @@ uci_wrapper.change_state( uci_state=uci_states.AVAILABLE ) - def add_storage_to_uci( self, name ): - """ Adds more storage to specified UCI - TODO""" - + def add_storage_to_uci( self, uci_wrapper ): + """ + Add an additional storage volume to specified UCI by creating the storage volume + on cloud provider, attaching it to currently running instance and adding it to + 'galaxyData' zpool on remote instance. + """ + conn = self.get_connection( uci_wrapper ) + + stores = uci_wrapper.get_all_stores_in_status( store_status.ADDING ) + for store in stores: + vol_size = store.size + availability_zone = uci_wrapper.get_uci_availability_zone() + log.info( "Adding storage volume to UCI '%s' in zone '%s' of size '%s'..." % ( uci_wrapper.get_name(), availability_zone, vol_size ) ) + + try: + vol = conn.create_volume( vol_size, availability_zone, snapshot=None ) + uci_wrapper.set_store_volume_id( store.id, vol.id ) + uci_wrapper.set_store_availability_zone( availability_zone, vol.id ) + log.debug( "New storage volume created: '%s'" % vol.id ) + except boto.exception.EC2ResponseError, e: + err = "EC2 response error while creating storage volume: " + str( e ) + log.error( err ) + uci_wrapper.set_store_error( err, store_id=vol.id ) + uci_wrapper.set_error( err, True ) + return + except Exception, ex: + err = "Error while creating storage volume: " + str( ex ) + log.error( err ) + uci_wrapper.set_error( err, True ) + return + + # Retrieve created volume again to get updated status + try: + vl = conn.get_all_volumes( [vol.id] ) + except boto.exception.EC2ResponseError, e: + err = "EC2 response error while retrieving (i.e., updating status) of just created storage volume '" + vol.id + "': " + str( e ) + log.error( err ) + uci_wrapper.set_store_error( err, store_id=vol.id ) + uci_wrapper.set_error( err, True ) + return + except Exception, ex: + err = "Error while retrieving (i.e., updating status) of just created storage volume '" + vol.id + "': " + str( ex ) + log.error( err ) + uci_wrapper.set_error( err, True ) + return + + # Wait for a while to ensure volume was created + if len( vl ) > 0: + vol_status = vl[0].status # Bc. only single vol is queried, reference it as 0th list element + for i in range( 30 ): + if vol_status != "available": + log.debug( "(%s) Updating volume status; current status: '%s'" % (i, vol_status ) ) + uci_wrapper.change_state( uci_state=vol_status ) + time.sleep(5) + vol_status = vl[0].status + if vol_status == "available": + log.debug( "(%s) New volume status '%s', continuing with file system adjustment." % (i, vol_status ) ) + uci_wrapper.set_store_status( vl[0].id, vol_status ) + break + if i is 29: + err = "Error while creating volume '"+vl[0].id+"'; stuck in state '"+vol_status+"'; deleting volume." + conn.delete_volume( vl[0].id ) + log.error( err ) + uci_wrapper.set_error( err, True ) + uci_wrapper.set_store_error( err, store_id=vol.id ) + conn.delete_volume( vl[0].id ) + uci_wrapper.set_store_deleted( vl[0].id ) + return + else: + err = "Volume '" + vol.id +"' not found by EC2 after being created." + log.error( err ) + uci_wrapper.set_store_error( err, store_id=vol.id ) + uci_wrapper.set_error( err, True ) + return + + # Get private key for given instance + pk = uci_wrapper.get_key_pair_material() + if pk == None: #If pk does not exist, create it + self.check_key_pair( uci_wrapper, conn ) + pk = uci_wrapper.get_key_pair_material() + + # Get working directory for this UCI and store pk into a file + wd = uci_wrapper.get_uci_working_directory() + if not os.path.exists( wd ): + os.mkdir( wd ) + pk_file_path = os.path.join( wd, "pk" ) + + if pk != None: + # Save private key to a file + pk_file = open( pk_file_path, "w" ) + pk_file.write( pk ) + pk_file.close() + else: + err = "ERROR: Private key not available for this UCI." + log.error( err ) + uci_wrapper.set_store_error( err, store_id=vol.id ) + uci_wrapper.set_error( err, True ) + return + + if os.path.exists( pk_file_path ): + # Change permissions of the file - this is required by later used ssh + os.chmod( pk_file_path, stat.S_IRUSR | stat.S_IWUSR ) + + # Get # of storage volumes associated with this UCI to know as which device to connect new volume to the instance + device_num = len( uci_wrapper.get_all_stores_in_status( store_status.IN_USE ) ) + 5 # First device num is 5, so all subsequent ones should follow + + # Get instance that the new storage volume is to be attached to. Although a list is returned, + # only 1 instance can be in 'adding-storage' state (because, for now, only 1 instance is assoc. with + # each UCI) and volume can be attached to only to it + il = uci_wrapper.get_instaces_in_state( instance_states.ADDING ) + if len( il ) > 0: + # Attach new volume to the instance + log.debug( "Attaching new storage volume '%s' to UCI '%s' as device '%s'" % + ( vol.id, uci_wrapper.get_name(), device_num ) ) + try: + vol_status = conn.attach_volume( vol.id, il[0].instance_id, device_num ) + except boto.exception.EC2ResponseError, e: + err = "Attaching just created storage volume '" + vol.id + "'to instance '" + \ + il[0].instance_id + "' as device '" + str( device_num ) + "' failed: " + str( e ) + log.error( err ) + uci_wrapper.set_store_error( err, store_id=vol.id ) + uci_wrapper.set_error( err, True ) + return + # For a while, keep checking attachment status of the new volume + for i in range(30): + log.debug( "Checking attachment status of new volume '%s': '%s'" % ( vol.id, vol_status ) ) + if vol_status == 'attached': + uci_wrapper.set_store_status( vol.id, vol_status ) + uci_wrapper.set_store_device( vol.id, device_num ) + break + if i == 29: + err = "Storage volume '" + vol.id + "' failed to attach to instance '" + il[0].instance_id + \ + "'. Manual check needed." + log.error( err ) + uci_wrapper.set_store_error( err, store_id=vol.id ) + uci_wrapper.set_error( err, False ) + return + + time.sleep(4) + vol_list = conn.get_all_volumes( [vol.id] ) + for v in vol_list: + vol_status = v.attachment_state() + + # Once storage volume is attached, add it to the zpool by issuing system level command + cmd = 'ssh -o StrictHostKeyChecking=no -i '+ pk_file_path +' root@'+il[0].public_dns+' "zpool add galaxyData c7d' + str( device_num )+'"' + log.debug( "Adding new storage volume to zpool cmd: %s" % cmd ) + stdout = os.system( cmd ) + if stdout != 0: + err = "Adding newly created storage volume to zpool on instance '" + il[0].instance_id + \ + "' failed. Error code: " + str( stdout ) + log.error( err ) + uci_wrapper.set_store_error( err, store_id=vol.id ) + uci_wrapper.set_error( err, False ) + return + else: + err = "No instance(s) found in 'adding-storage' state. New disk not added to UCI's zpool." + log.error( err ) + uci_wrapper.set_store_error( err, store_id=vol.id ) + uci_wrapper.set_error( err, True ) + return + + # Update UCI's total storage size + uci_wrapper.set_uci_total_size( uci_wrapper.get_uci_total_size() + vol.size ) + # Reset UCI's and instance's state + uci_wrapper.change_state( uci_state=uci_states.RUNNING, instance_id=il[0].instance_id, i_state=instance_states.RUNNING ) + log.debug( "Successfully added storage volume '%s' to UCI '%s'." % ( vol.id, uci_wrapper.get_name() ) ) + def dummy_start_uci( self, uci_wrapper ): uci = uci_wrapper.get_uci() - log.debug( "Would be starting instance '%s'" % uci.name ) - uci_wrapper.change_state( uci_state.PENDING ) + log.debug( "Dummy start UCI '%s'" % uci.name ) + + +# uci_wrapper.change_state( uci_state.PENDING ) # log.debug( "Sleeping a bit... (%s)" % uci.name ) # time.sleep(20) # log.debug( "Woke up! (%s)" % uci.name ) @@ -418,10 +580,25 @@ if uci_wrapper.get_uci_state() != uci_states.ERROR: # Start an instance log.debug( "Starting instance for UCI '%s'" % uci_wrapper.get_name() ) - #TODO: Once multiple volumes can be attached to a single instance, update 'userdata' composition - userdata = uci_wrapper.get_store_volume_id()+"|"+uci_wrapper.get_access_key()+"|"+uci_wrapper.get_secret_key() + #TODO: Once multiple volumes can be attached to a single instance, update 'userdata' composition + # Compose user data; for storage volumes, separate multiple volumes with a colon (:) ensuring that + # the last volume in the list is not followed by a colon. + stores = uci_wrapper.get_all_stores() + volume_ids = "" + if len( stores ) > 0: + for i, store in enumerate( stores ): + volume_ids += store.volume_id + if i < len( stores )-1: + volume_ids += ":" + else: + err = "No storage volumes found that are associated with UCI '%s'" + uci_wrapper.get_name() + log.error( err ) + uci_wrapper.set_error( err, True ) + return + userdata = volume_ids+"|"+uci_wrapper.get_access_key()+"|"+uci_wrapper.get_secret_key() log.debug( "Using following command: conn.run_instances( image_id='%s', key_name='%s', security_groups=['%s'], user_data=[OMITTED], instance_type='%s', placement='%s' )" % ( mi_id, uci_wrapper.get_key_pair_name(), self.security_group, uci_wrapper.get_instance_type( i_index ), uci_wrapper.get_uci_availability_zone() ) ) + # Start an instance reservation = None try: reservation = conn.run_instances( image_id=mi_id, @@ -454,7 +631,10 @@ uci_wrapper.change_state( s, i_id, s ) uci_wrapper.set_security_group_name( self.security_group, i_id=i_id ) vol_id = uci_wrapper.get_store_volume_id( store_id=0 ) # TODO: Once more that one vol/UCI is allowed, update this! - uci_wrapper.set_store_status( vol_id, store_status.WAITING ) + # Following line is pointless bc. general update updates status of volume to 'available' + # before it actually connects to starting instance... This has been dealt w/ in general update method + #uci_wrapper.set_store_status( vol_id, store_status.WAITING ) + uci_wrapper.set_store_instance( vol_id, i_id ) log.debug( "Instance of UCI '%s' started, current state: '%s'" % ( uci_wrapper.get_name(), uci_wrapper.get_uci_state() ) ) except boto.exception.EC2ResponseError, e: err = "EC2 response error when retrieving instance information for UCI '" + uci_wrapper.get_name() + "': " + str( e ) @@ -571,19 +751,30 @@ .all() for inst in instances: if self.type == inst.uci.credentials.provider.type: - log.debug( "[%s] Running general status update on instance '%s'" % ( inst.uci.credentials.provider.type, inst.instance_id ) ) + log.debug( "[%s] Running general status update on instance '%s'" + % ( inst.uci.credentials.provider.type, inst.instance_id ) ) self.update_instance( inst ) + # Update storage volume(s) associated with current instance + stores = self.sa_session.query( model.CloudStore ) \ + .filter_by( uci_id=inst.uci_id, deleted=False ) \ + .all() + for store in stores: + if self.type == store.uci.credentials.provider.type: # and store.volume_id != None: + log.debug( "[%s] Running general status update on store with local database ID: '%s'" + % ( store.uci.credentials.provider.type, store.id ) ) + self.update_store( store ) # Update storage volume(s) stores = self.sa_session.query( model.CloudStore ) \ - .filter( or_( model.CloudStore.table.c.status==store_status.IN_USE, - model.CloudStore.table.c.status==store_status.CREATING, - model.CloudStore.table.c.status==store_status.WAITING, + .filter( or_( model.CloudStore.table.c.status==store_status.CREATING, +# model.CloudStore.table.c.status==store_status.IN_USE, +# model.CloudStore.table.c.status==store_status.WAITING, model.CloudStore.table.c.status==None ) ) \ .all() for store in stores: if self.type == store.uci.credentials.provider.type: # and store.volume_id != None: - log.debug( "[%s] Running general status update on store with local database ID: '%s'" % ( store.uci.credentials.provider.type, store.id ) ) + log.debug( "[%s] Running general status update on store with local database ID: '%s'" + % ( store.uci.credentials.provider.type, store.id ) ) self.update_store( store ) # else: # log.error( "[%s] There exists an entry for UCI (%s) storage volume without an ID. Storage volume might have been created with " @@ -668,7 +859,8 @@ for i, cInst in enumerate( r.instances ): try: s = cInst.update() - log.debug( "Checking state of cloud instance '%s' associated with UCI '%s' and reservation '%s'. State='%s'" % ( cInst, uci.name, r, s ) ) + log.debug( "Checking state of cloud instance '%s' associated with UCI '%s' " \ + "and reservation '%s'. State='%s'" % ( cInst, uci.name, r, s ) ) if s != inst.state: inst.state = s self.sa_session.add( inst ) @@ -693,7 +885,8 @@ self.sa_session.add( inst ) self.sa_session.flush() except boto.exception.EC2ResponseError, e: - err = "Updating instance status from cloud failed for UCI '"+ uci.name + "' during general status update: " + str( e ) + err = "Updating instance status from cloud failed for UCI '"+ uci.name + \ + "' during general status update: " + str( e ) log.error( err ) uci.error = err uci.state = uci_states.ERROR @@ -715,7 +908,7 @@ # Get reservations handle for given store try: - log.debug( "Updating storage volume command: vl = conn.get_all_volumes( [%s] )" % store.volume_id ) + log.debug( "Retrieving reference to storage volume '%s' during update..." % store.volume_id ) vl = conn.get_all_volumes( [store.volume_id] ) except boto.exception.EC2ResponseError, e: err = "Retrieving volume(s) from cloud failed for UCI '"+ uci.name + "' during general status update: " + str( e ) @@ -729,7 +922,7 @@ # Update store status in local DB with info from cloud provider if len(vl) > 0: try: - log.debug( "Storage volume '%s' current status: '%s'" % (store.volume_id, vl[0].status ) ) + log.debug( "General status update for storage volume '%s'; current status: '%s'" % (store.volume_id, vl[0].status ) ) if store.status != vl[0].status: # In case something failed during creation of UCI but actual storage volume was created and yet # UCI state remained as 'new', try to remedy this by updating UCI state here @@ -746,21 +939,26 @@ store.status = vl[0].status self.sa_session.add( store ) self.sa_session.flush() - if store.inst != None: - if store.inst.instance_id != vl[0].instance_id: - store.inst.instance_id = vl[0].instance_id - self.sa_session.add( store ) - self.sa_session.flush() - if store.attach_time != vl[0].attach_time: - store.attach_time = vl[0].attach_time - self.sa_session.add( store ) - self.sa_session.flush() - if store.device != vl[0].device: - store.device = vl[0].device - self.sa_session.add( store ) - self.sa_session.flush() + # Boto does not seem to be reporting these values although fields exist so comment them out... +# log.debug( "vl[0].instance_id: '%s'" % vl[0].instance_id ) +# if store.inst != None: +# if store.inst.instance_id != vl[0].instance_id: +# store.inst.instance_id = vl[0].instance_id +# self.sa_session.add( store ) +# self.sa_session.flush() +# log.debug( "vl[0].attach_time: '%s'" % vl[0].attach_time ) +# if store.attach_time != vl[0].attach_time: +# store.attach_time = vl[0].attach_time +# self.sa_session.add( store ) +# self.sa_session.flush() +## log.debug( "vl[0].device: '%s'" % vl[0].device ) +# if store.device != vl[0].device: +# store.device = vl[0].device +# self.sa_session.add( store ) +# self.sa_session.flush() except boto.exception.EC2ResponseError, e: - err = "Updating status of volume(s) from cloud failed for UCI '"+ uci.name + "' during general status update: " + str( e ) + err = "Updating status of volume(s) from cloud failed for UCI '"+ uci.name + \ + "' during general status update: " + str( e ) log.error( err ) uci.error = err uci.state = uci_states.ERROR @@ -768,7 +966,7 @@ self.sa_session.flush() return None else: - err = "No storage volumes returned by cloud provider on general update" + err = "No storage volumes returned by cloud provider on general update for volume with id: " + store.volume_id log.error( "%s for UCI '%s'" % ( err, uci.name ) ) store.status = store_status.ERROR store.error = err diff -r f4654abcec1e -r 1d93eb88c93f lib/galaxy/cloud/providers/eucalyptus.py --- a/lib/galaxy/cloud/providers/eucalyptus.py Fri Nov 20 11:03:41 2009 -0500 +++ b/lib/galaxy/cloud/providers/eucalyptus.py Mon Nov 30 16:36:38 2009 -0500 @@ -30,6 +30,8 @@ SUBMITTED = "submitted", SHUTTING_DOWN_UCI = "shutting-downUCI", SHUTTING_DOWN = "shutting-down", + ADD_STORAGE_UCI = "add-storageUCI", + ADD_STORAGE = "add-storage", AVAILABLE = "available", RUNNING = "running", PENDING = "pending", @@ -43,6 +45,7 @@ TERMINATED = "terminated", SUBMITTED = "submitted", RUNNING = "running", + ADDING = "adding-storage", PENDING = "pending", SHUTTING_DOWN = "shutting-down", ERROR = "error" @@ -51,6 +54,7 @@ store_status = Bunch( WAITING = "waiting", IN_USE = "in-use", + ADDING = "adding", CREATING = "creating", DELETED = 'deleted', ERROR = "error" @@ -121,6 +125,8 @@ self.stop_uci( uci_wrapper ) elif uci_state==uci_states.SNAPSHOT: self.snapshot_uci( uci_wrapper ) + elif uci_state==uci_states.ADD_STORAGE: + self.add_storage_to_uci( uci_wrapper ) except: log.exception( "Uncaught exception executing cloud request." ) cnt += 1 @@ -245,9 +251,16 @@ log.info( "Availability zone for UCI (i.e., storage volume) was not selected, using default zone: %s" % self.zone ) uci_wrapper.set_store_availability_zone( self.zone ) - log.debug( "Creating volume; using command: conn.create_volume( %s, '%s', snapshot=None )" % ( uci_wrapper.get_store_size( 0 ), uci_wrapper.get_uci_availability_zone() )) - vol = conn.create_volume( uci_wrapper.get_store_size( 0 ), uci_wrapper.get_uci_availability_zone(), snapshot=None ) - uci_wrapper.set_store_volume_id( 0, vol.id ) +# log.debug( "Creating volume; using command: conn.create_volume( %s, '%s', snapshot=None )" % ( uci_wrapper.get_store_size( 0 ), uci_wrapper.get_uci_availability_zone() )) +# vol = conn.create_volume( uci_wrapper.get_store_size( 0 ), uci_wrapper.get_uci_availability_zone(), snapshot=None ) +# uci_wrapper.set_store_volume_id( 0, vol.id ) + store = uci_wrapper.get_all_stores_in_status( store_status.ADDING )[0] # Because at UCI creation time only 1 storage volume can be created, reference it directly + + log.info( "Creating storage volume in zone '%s' of size '%s'..." % ( uci_wrapper.get_uci_availability_zone(), store.size ) ) + # Because only 1 storage volume may be created at UCI config time, index of this storage volume in local Galaxy DB w.r.t + # current UCI is 0, so reference it in following methods + vol = conn.create_volume( store.size, uci_wrapper.get_uci_availability_zone(), snapshot=None ) + uci_wrapper.set_store_volume_id( store.id, vol.id ) # Retrieve created volume again to get updated status try: @@ -364,6 +377,7 @@ def add_storage_to_uci( self, uci_wrapper ): """ Adds more storage to specified UCI """ + uci_wrapper.set_error( "Adding storage to eucalyptus-based clouds is not yet supported.", True ) def dummy_start_uci( self, uci_wrapper ): diff -r f4654abcec1e -r 1d93eb88c93f lib/galaxy/web/controllers/cloud.py --- a/lib/galaxy/web/controllers/cloud.py Fri Nov 20 11:03:41 2009 -0500 +++ b/lib/galaxy/web/controllers/cloud.py Mon Nov 30 16:36:38 2009 -0500 @@ -41,6 +41,8 @@ SUBMITTED = "submitted", SHUTTING_DOWN_UCI = "shutting-downUCI", SHUTTING_DOWN = "shutting-down", + ADD_STORAGE_UCI = "add-storageUCI", + ADD_STORAGE = "add-storage", AVAILABLE = "available", RUNNING = "running", PENDING = "pending", @@ -54,13 +56,16 @@ TERMINATED = "terminated", SUBMITTED = "submitted", RUNNING = "running", + ADDING = "adding-storage", PENDING = "pending", SHUTTING_DOWN = "shutting-down", ERROR = "error" ) store_status = Bunch( + WAITING = "waiting", IN_USE = "in-use", + ADDING = "adding", CREATING = "creating", DELETED = 'deleted', ERROR = "error" @@ -108,7 +113,9 @@ model.UCI.table.c.state==uci_states.SUBMITTED, model.UCI.table.c.state==uci_states.SUBMITTED_UCI, model.UCI.table.c.state==uci_states.SHUTTING_DOWN, - model.UCI.table.c.state==uci_states.SHUTTING_DOWN_UCI ) ) \ + model.UCI.table.c.state==uci_states.SHUTTING_DOWN_UCI, + model.UCI.table.c.state==uci_states.ADD_STORAGE, + model.UCI.table.c.state==uci_states.ADD_STORAGE_UCI ) ) \ .order_by( desc( model.UCI.table.c.update_time ) ) \ .all() @@ -201,7 +208,8 @@ storage.user = user storage.uci = uci storage.size = volSize - storage.availability_zone = zone + storage.availability_zone = zone + storage.status = store_status.ADDING # Persist session = trans.sa_session session.add( uci ) @@ -306,6 +314,8 @@ ( uci.state != uci_states.ERROR ) and \ ( uci.state != uci_states.SHUTTING_DOWN_UCI ) and \ ( uci.state != uci_states.SHUTTING_DOWN ) and \ + ( uci.state != uci_states.ADD_STORAGE_UCI ) and \ + ( uci.state != uci_states.ADD_STORAGE ) and \ ( uci.state != uci_states.AVAILABLE ): uci.state = uci_states.SHUTTING_DOWN_UCI session = trans.sa_session @@ -496,13 +506,53 @@ @web.expose @web.require_login( "add instance storage" ) - def add_storage( self, trans, id ): - instance = get_uci( trans, id ) + def add_storage( self, trans, id, vol_size=None ): + error = None + uci = get_uci( trans, id ) + stores = get_stores_in_status( trans, uci, store_status.IN_USE ) + # Start adding of storage making sure given UCI is running and that at least one + # storage volume is attached to it (this is needed to by cloud controller to know + # as which device to attach the new storage volume) + if uci.state == uci_states.RUNNING and len( stores ) > 0: + if vol_size is not None: + try: + vol_size = int( vol_size ) + except ValueError: + error = "Volume size must be integer value between 1 and 1000." + + if not error: + user = trans.get_user() + + storage = model.CloudStore() + storage.user = user + storage.uci = uci + storage.size = vol_size + storage.status = store_status.ADDING + + # Set state of instance - NOTE that this code will only work (with code in cloud controller) + # for scenario where a UCI is associated with *1* compute instance!!! + instances = get_instances( trans, uci ) + instances.state = instance_states.ADDING + + uci.state = uci_states.ADD_STORAGE_UCI + # Persist + session = trans.sa_session + session.add( instances ) + session.add( storage ) + session.add( uci ) + session.flush() + # Log and display the management page + trans.log_event( "User added storage volume to UCI: '%s'" % uci.name ) + trans.set_message( "Adding of storage to instance '%s' initiated." % uci.name ) + return self.list( trans ) + else: + error( "Storage can only be added to instances that are in state 'RUNNING' with existing " \ + "storage volume(s) already attached." ) - error( "Adding storage to instance '%s' is not supported yet." % instance.name ) - - return self.list( trans ) + return trans.show_form( + web.FormBuilder( url_for( id=trans.security.encode_id(uci.id) ), "Add storage to an instance", submit_text="Add" ) + .add_text( "vol_size", "Storage size (1-1000 GB)", value='', error=error ) ) # ----- Image methods ----- @web.expose @@ -1034,6 +1084,7 @@ dict = {} dict['id'] = uci.id dict['state'] = uci.state + dict['total_size'] = uci.total_size if uci.error != None: dict['error'] = str( uci.error ) else: @@ -1136,19 +1187,30 @@ def get_stores( trans, uci ): """ - Get stores objects that are connected to uci object + Get stores objects that are associated with given uci and are not in 'error' status """ user = trans.get_user() stores = trans.sa_session.query( model.CloudStore ) \ - .filter_by( user=user, uci_id=uci.id ) \ + .filter_by( user=user, uci_id=uci.id, deleted=False ) \ .filter( model.CloudStore.table.c.status != store_status.ERROR ) \ .all() return stores +def get_stores_in_status( trans, uci, status ): + """ + Get stores objects that are associated with given uci and are not have given status + """ + user = trans.get_user() + stores = trans.sa_session.query( model.CloudStore ) \ + .filter_by( user=user, uci_id=uci.id, status=status ) \ + .all() + + return stores + def get_instances( trans, uci ): """ - Get objects of instances that are pending or running and are connected to uci object + Get objects of instances that are pending or running and are connected to the given uci object """ user = trans.get_user() instances = trans.sa_session.query( model.CloudInstance ) \ @@ -1159,6 +1221,17 @@ return instances +def get_instances_in_state( trans, uci, state ): + """ + Get objects of instances that are in specified state and are connected to the given uci object + """ + user = trans.get_user() + instances = trans.sa_session.query( model.CloudInstance ) \ + .filter_by( user=user, uci_id=uci.id, state=state ) \ + .all() + + return instances + def get_connection( trans, creds ): """ Establishes cloud connection using user's credentials diff -r f4654abcec1e -r 1d93eb88c93f templates/cloud/configure_cloud.mako --- a/templates/cloud/configure_cloud.mako Fri Nov 20 11:03:41 2009 -0500 +++ b/templates/cloud/configure_cloud.mako Mon Nov 30 16:36:38 2009 -0500 @@ -79,7 +79,8 @@ }); } - // Update 'state' and 'time alive' fields + // Update 'size', 'state' and 'time alive' fields + $(elem + "-size").text( data[i].total_size ); $(elem + "-state").text( data[i].state ); if ( new_state != 'error' ) { // Because 'error' state is handled as a JS link, don't include it in update $(elem + "-state-p").text( data[i].state ); @@ -219,7 +220,7 @@ ${liveInstance.name} (${liveInstance.credentials.name}) <a id="li-${i}-popup" class="popup-arrow" style="display: none;">▼</a> </td> - <td>${str(liveInstance.total_size)}</td> + <td id="${ liveInstance.id }-size">${str(liveInstance.total_size)}</td> <td id="${ liveInstance.id }-state">${str(liveInstance.state)}</td> <td id="${ liveInstance.id }-launch_time"> ##${str(liveInstance.launch_time)[:16]} @@ -251,7 +252,8 @@ <a class="action-button" confirm="Are you sure you want to stop instance '${liveInstance.name}'?" href="${h.url_for( action='stop', id=trans.security.encode_id(liveInstance.id) )}">Stop</a> <a class="action-button" href="${h.url_for( action='rename_uci', id=trans.security.encode_id(liveInstance.id) )}">Rename</a> <a class="action-button" href="${h.url_for( action='view_uci_details', id=trans.security.encode_id(liveInstance.id) )}">View details</a> - <a class="action-button" href="${h.url_for( action='uci_usage_report', id=trans.security.encode_id(liveInstance.id) )}">Usage report</a> + <a class="action-button" href="${h.url_for( action='add_storage', id=trans.security.encode_id(liveInstance.id) )}">Add storage</a> + <a class="action-button" href="${h.url_for( action='uci_usage_report', id=trans.security.encode_id(liveInstance.id) )}">Usage report</a> </div> </td> </tr> @@ -315,14 +317,13 @@ </td> <td> <div popupmenu="pi-${i}-popup"> - <a class="action-button" href="${h.url_for( action='start', id=trans.security.encode_id(prevInstance.id), type='m1.small' )}"> Start m1.small</a> + <a class="action-button" href="${h.url_for( action='uci_usage_report', id=trans.security.encode_id(prevInstance.id) )}">Usage report</a> + <a class="action-button" href="${h.url_for( action='rename_uci', id=trans.security.encode_id(prevInstance.id) )}">Rename</a> + <a class="action-button" href="${h.url_for( action='start', id=trans.security.encode_id(prevInstance.id), type='m1.small' )}"> Start m1.small</a> <a class="action-button" href="${h.url_for( action='start', id=trans.security.encode_id(prevInstance.id), type='c1.medium' )}"> Start c1.medium</a> - <a class="action-button" href="${h.url_for( action='rename_uci', id=trans.security.encode_id(prevInstance.id) )}">Rename</a> - <a class="action-button" href="${h.url_for( action='uci_usage_report', id=trans.security.encode_id(prevInstance.id) )}">Usage report</a> - <a class="action-button" href="${h.url_for( action='create_snapshot', id=trans.security.encode_id(prevInstance.id) )}">Create snapshot</a> + <a class="action-button" href="${h.url_for( action='create_snapshot', id=trans.security.encode_id(prevInstance.id) )}">Create snapshot</a> <a class="action-button" href="${h.url_for( action='view_snapshots', id=trans.security.encode_id(prevInstance.id) )}">View snapshots</a> - <a class="action-button" href="${h.url_for( action='add_storage', id=trans.security.encode_id(prevInstance.id) )}" target="_parent">Add storage</a> - <a class="action-button" confirm="Are you sure you want to delete instance '${prevInstance.name}'? This will delete all of your data assocaiated with this instance!" href="${h.url_for( action='delete_uci', id=trans.security.encode_id(prevInstance.id) )}">Delete</a> + <a class="action-button" confirm="Are you sure you want to delete instance '${prevInstance.name}'? This will delete all of your data assocaiated with this instance!" href="${h.url_for( action='delete_uci', id=trans.security.encode_id(prevInstance.id) )}">Delete</a> </div> </td> </tr>

1 0

[hg] galaxy 3136: Added some error checking to pileup_parser too...
by Greg Von Kuster 02 Dec '09

02 Dec '09

details: http://www.bx.psu.edu/hg/galaxy/rev/fe853d8e062d changeset: 3136:fe853d8e062d user: Kelly Vincent <kpvincent(a)bx.psu.edu> date: Tue Dec 01 16:18:25 2009 -0500 description: Added some error checking to pileup_parser tool so that it exits with a meaningful message if the user tries to do some 6-column work on a 10-column file, or vice versa. diffstat: tools/samtools/pileup_interval.py | 16 ++++++++++++++-- 1 files changed, 14 insertions(+), 2 deletions(-) diffs (40 lines): diff -r eb317734e391 -r fe853d8e062d tools/samtools/pileup_interval.py --- a/tools/samtools/pileup_interval.py Tue Dec 01 11:49:07 2009 -0500 +++ b/tools/samtools/pileup_interval.py Tue Dec 01 16:18:25 2009 -0500 @@ -1,7 +1,7 @@ #! /usr/bin/python """ -Creates a pileup file from a bam file and a reference. +Condenses pileup format into ranges of bases. usage: %prog [options] -i, --input=i: Input pileup file @@ -18,6 +18,7 @@ from galaxy import eggs import pkg_resources; pkg_resources.require( "bx-python" ) from bx.cookbook import doc_optparse +import sys def stop_err( msg ): sys.stderr.write( msg ) @@ -56,7 +57,18 @@ bases = [] while inLine.strip() != '': lineParts = inLine.split('\t') - seq, loc, base, cov = lineParts[seqIndex], int(lineParts[locIndex]), lineParts[baseIndex], int(lineParts[covIndex]) + try: + seq, loc, base, cov = lineParts[seqIndex], int(lineParts[locIndex]), lineParts[baseIndex], int(lineParts[covIndex]) + except IndexError, ei: + if options.format == 'ten': + stop_err( 'It appears that you have selected 10 columns while your file has 6. Make sure that the number of columns you specify matches the number in your file.\n' + str( ei ) ) + else: + stop_err( 'There appears to be something wrong with your column index values.\n' + str( ei ) ) + except ValueError, ev: + if options.format == 'six': + stop_err( 'It appears that you have selected 6 columns while your file has 10. Make sure that the number of columns you specify matches the number in your file.\n' + str( ev ) ) + else: + stop_err( 'There appears to be something wrong with your column index values.\n' + str( ev ) ) # strout += str(startLoc) + '\n' # strout += str(bases) + '\n' # strout += '%s\t%s\t%s\t%s\n' % (seq, loc, base, cov)

1 0

[hg] galaxy 3133: Merge
by Greg Von Kuster 02 Dec '09

02 Dec '09

details: http://www.bx.psu.edu/hg/galaxy/rev/e75b55cc9e08 changeset: 3133:e75b55cc9e08 user: Enis Afgan <afgane(a)gmail.com> date: Tue Dec 01 09:53:36 2009 -0500 description: Merge diffstat: lib/galaxy/config.py | 1 + lib/galaxy/model/__init__.py | 10 +++++ lib/galaxy/model/mapping.py | 13 ++++++ lib/galaxy/model/migrate/versions/0029_user_actions.py | 47 +++++++++++++++++++++++ lib/galaxy/web/controllers/dataset.py | 2 +- lib/galaxy/web/controllers/history.py | 2 +- lib/galaxy/web/controllers/tag.py | 24 ++++++++--- lib/galaxy/web/framework/__init__.py | 17 ++++++++ lib/galaxy/web/framework/helpers/grids.py | 13 +++++- templates/dataset/edit_attributes.mako | 2 +- templates/history/view.mako | 2 +- templates/root/history.mako | 2 +- templates/tagging_common.mako | 8 ++-- universe_wsgi.ini.sample | 3 + 14 files changed, 128 insertions(+), 18 deletions(-) diffs (335 lines): diff -r efed28fa6a5e -r e75b55cc9e08 lib/galaxy/config.py --- a/lib/galaxy/config.py Mon Nov 30 16:47:18 2009 -0500 +++ b/lib/galaxy/config.py Tue Dec 01 09:53:36 2009 -0500 @@ -79,6 +79,7 @@ self.use_heartbeat = string_as_bool( kwargs.get( 'use_heartbeat', 'False' ) ) self.use_memdump = string_as_bool( kwargs.get( 'use_memdump', 'False' ) ) self.log_memory_usage = string_as_bool( kwargs.get( 'log_memory_usage', 'False' ) ) + self.log_actions = string_as_bool( kwargs.get( 'log_actions', 'False' ) ) self.log_events = string_as_bool( kwargs.get( 'log_events', 'False' ) ) self.ucsc_display_sites = kwargs.get( 'ucsc_display_sites', "main,test,archaea,ucla" ).lower().split(",") self.gbrowse_display_sites = kwargs.get( 'gbrowse_display_sites', "main,test,tair" ).lower().split(",") diff -r efed28fa6a5e -r e75b55cc9e08 lib/galaxy/model/__init__.py --- a/lib/galaxy/model/__init__.py Mon Nov 30 16:47:18 2009 -0500 +++ b/lib/galaxy/model/__init__.py Tue Dec 01 09:53:36 2009 -0500 @@ -1441,6 +1441,16 @@ def __init__( self, name=None, value=None ): self.name = name self.value = value + +class UserAction( object ): + def __init__( self, id=None, create_time=None, user_id=None, session_id=None, action=None, params=None, context=None): + self.id = id + self.create_time = create_time + self.user_id = user_id + self.session_id = session_id + self.action = action + self.params = params + self.context = context ## ---- Utility methods ------------------------------------------------------- diff -r efed28fa6a5e -r e75b55cc9e08 lib/galaxy/model/mapping.py --- a/lib/galaxy/model/mapping.py Mon Nov 30 16:47:18 2009 -0500 +++ b/lib/galaxy/model/mapping.py Tue Dec 01 09:53:36 2009 -0500 @@ -743,6 +743,15 @@ Column( "name", Unicode( 255 ), index=True), Column( "value", Unicode( 1024 ) ) ) +UserAction.table = Table( "user_action", metadata, + Column( "id", Integer, primary_key=True ), + Column( "create_time", DateTime, default=now ), + Column( "user_id", Integer, ForeignKey( "galaxy_user.id" ), index=True ), + Column( "session_id", Integer, ForeignKey( "galaxy_session.id" ), index=True ), + Column( "action", Unicode( 255 ) ), + Column( "context", Unicode( 512 ) ), + Column( "params", Unicode( 1024 ) ) ) + # With the tables defined we can define the mappers and setup the # relationships between the model objects. @@ -1237,6 +1246,10 @@ assign_mapper( context, UserPreference, UserPreference.table, properties = {} ) + +assign_mapper( context, UserAction, UserAction.table, + properties = dict( user=relation( User.mapper ) ) + ) def db_next_hid( self ): """ diff -r efed28fa6a5e -r e75b55cc9e08 lib/galaxy/model/migrate/versions/0029_user_actions.py --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/lib/galaxy/model/migrate/versions/0029_user_actions.py Tue Dec 01 09:53:36 2009 -0500 @@ -0,0 +1,47 @@ +""" +This migration script adds a user actions table to Galaxy. +""" + +from sqlalchemy import * +from migrate import * + +import datetime +now = datetime.datetime.utcnow + +import logging +log = logging.getLogger( __name__ ) + +metadata = MetaData( migrate_engine ) + +def display_migration_details(): + print "" + print "This migration script adds a user actions table to Galaxy." + print "" + + +# New table to store user actions. +UserAction_table = Table( "user_action", metadata, + Column( "id", Integer, primary_key=True ), + Column( "create_time", DateTime, default=now ), + Column( "user_id", Integer, ForeignKey( "galaxy_user.id" ), index=True ), + Column( "session_id", Integer, ForeignKey( "galaxy_session.id" ), index=True ), + Column( "action", Unicode( 255 ) ), + Column( "context", Unicode( 512 ) ), + Column( "params", Unicode( 1024 ) ) ) + +def upgrade(): + display_migration_details() + metadata.reflect() + try: + UserAction_table.create() + except Exception, e: + print str(e) + log.debug( "Creating user_action table failed: %s" % str( e ) ) + +def downgrade(): + metadata.reflect() + try: + UserAction_table.drop() + except Exception, e: + print str(e) + log.debug( "Dropping user_action table failed: %s" % str( e ) ) \ No newline at end of file diff -r efed28fa6a5e -r e75b55cc9e08 lib/galaxy/web/controllers/dataset.py --- a/lib/galaxy/web/controllers/dataset.py Mon Nov 30 16:47:18 2009 -0500 +++ b/lib/galaxy/web/controllers/dataset.py Tue Dec 01 09:53:36 2009 -0500 @@ -87,7 +87,7 @@ link=( lambda item: iff( item.history.deleted, None, dict( operation="switch", id=item.id ) ) ), filterable="advanced" ), HistoryColumn( "History", key="history", link=( lambda item: iff( item.history.deleted, None, dict( operation="switch_history", id=item.id ) ) ) ), - grids.TagsColumn( "Tags", "tags", model.HistoryDatasetAssociation, model.HistoryDatasetAssociationTagAssociation, filterable="advanced" ), + grids.TagsColumn( "Tags", "tags", model.HistoryDatasetAssociation, model.HistoryDatasetAssociationTagAssociation, filterable="advanced", grid_name="HistoryDatasetAssocationListGrid" ), StatusColumn( "Status", key="deleted", attach_popup=False ), grids.GridColumn( "Created", key="create_time", format=time_ago ), grids.GridColumn( "Last Updated", key="update_time", format=time_ago ), diff -r efed28fa6a5e -r e75b55cc9e08 lib/galaxy/web/controllers/history.py --- a/lib/galaxy/web/controllers/history.py Mon Nov 30 16:47:18 2009 -0500 +++ b/lib/galaxy/web/controllers/history.py Tue Dec 01 09:53:36 2009 -0500 @@ -94,7 +94,7 @@ link=( lambda history: iff( history.deleted, None, dict( operation="Switch", id=history.id ) ) ), attach_popup=True, filterable="advanced" ), DatasetsByStateColumn( "Datasets (by state)", ncells=4 ), - grids.TagsColumn( "Tags", "tags", model.History, model.HistoryTagAssociation, filterable="advanced"), + grids.TagsColumn( "Tags", "tags", model.History, model.HistoryTagAssociation, filterable="advanced", grid_name="HistoryListGrid" ), StatusColumn( "Status", attach_popup=False ), grids.GridColumn( "Created", key="create_time", format=time_ago ), grids.GridColumn( "Last Updated", key="update_time", format=time_ago ), diff -r efed28fa6a5e -r e75b55cc9e08 lib/galaxy/web/controllers/tag.py --- a/lib/galaxy/web/controllers/tag.py Mon Nov 30 16:47:18 2009 -0500 +++ b/lib/galaxy/web/controllers/tag.py Tue Dec 01 09:53:36 2009 -0500 @@ -28,28 +28,38 @@ @web.expose @web.require_login( "Add tag to an item." ) - def add_tag_async( self, trans, id=None, item_class=None, new_tag=None ): + def add_tag_async( self, trans, id=None, item_class=None, new_tag=None, context=None ): """ Add tag to an item. """ - item = self._get_item(trans, item_class, trans.security.decode_id(id)) - self._do_security_check(trans, item) + # Check that user owns item. + item = self._get_item(trans, item_class, trans.security.decode_id( id ) ) + self._do_security_check( trans, item ) + # Apply tag. self.tag_handler.apply_item_tags( trans.sa_session, item, new_tag.encode('utf-8') ) trans.sa_session.flush() + # Log. + params = dict( item_id=item.id, item_class=item_class, tag=new_tag) + trans.log_action( unicode( "tag"), context, params ) + @web.expose @web.require_login( "Remove tag from an item." ) - def remove_tag_async( self, trans, id=None, item_class=None, tag_name=None ): + def remove_tag_async( self, trans, id=None, item_class=None, tag_name=None, context=None ): """ Remove tag from an item. """ + + # Check that user owns item. item = self._get_item(trans, item_class, trans.security.decode_id(id)) - self._do_security_check(trans, item) + # Remove tag. self.tag_handler.remove_item_tag( trans, item, tag_name.encode('utf-8') ) - #print tag_name - #print unicode(tag_name) trans.sa_session.flush() + # Log. + params = dict( item_id=item.id, item_class=item_class, tag=tag_name) + trans.log_action( unicode( "untag"), context, params ) + # Retag an item. All previous tags are deleted and new tags are applied. @web.expose @web.require_login( "Apply a new set of tags to an item; previous tags are deleted." ) diff -r efed28fa6a5e -r e75b55cc9e08 lib/galaxy/web/framework/__init__.py --- a/lib/galaxy/web/framework/__init__.py Mon Nov 30 16:47:18 2009 -0500 +++ b/lib/galaxy/web/framework/__init__.py Tue Dec 01 09:53:36 2009 -0500 @@ -10,6 +10,7 @@ import base import pickle from galaxy import util +from galaxy.util.json import to_json_string pkg_resources.require( "simplejson" ) import simplejson @@ -170,6 +171,22 @@ to allow migration toward a more SQLAlchemy 0.4 style of use. """ return self.app.model.context.current + def log_action( self, action, context, params): + """ + Application-level logging of user actions. + """ + if self.app.config.log_actions: + action = self.app.model.UserAction(action=action, context=context, params=unicode( to_json_string( params ) ) ) + try: + action.user = self.user + except: + action.user = None + try: + action.session_id = self.galaxy_session.id + except: + action.session_id = None + self.sa_session.add( action ) + self.sa_session.flush() def log_event( self, message, tool_id=None, **kwargs ): """ Application level logging. Still needs fleshing out (log levels and such) diff -r efed28fa6a5e -r e75b55cc9e08 lib/galaxy/web/framework/helpers/grids.py --- a/lib/galaxy/web/framework/helpers/grids.py Mon Nov 30 16:47:18 2009 -0500 +++ b/lib/galaxy/web/framework/helpers/grids.py Tue Dec 01 09:53:36 2009 -0500 @@ -200,6 +200,13 @@ trans.get_user().preferences[pref_name] = unicode( to_json_string( sort_key ) ) trans.sa_session.flush() + # Log grid view. + context = unicode( self.__class__.__name__ ) + params = cur_filter_dict.copy() + params['sort'] = sort_key + params['async'] = ( 'async' in kwargs ) + trans.log_action( unicode( "grid.view"), context, params ) + # Render grid. def url( *args, **kwargs ): # Only include sort/filter arguments if not linking to another @@ -340,17 +347,19 @@ # Generic column that supports tagging. class TagsColumn( TextColumn ): - def __init__( self, col_name, key, model_class, model_tag_association_class, filterable ): + def __init__( self, col_name, key, model_class, model_tag_association_class, filterable, grid_name=None ): GridColumn.__init__(self, col_name, key=key, model_class=model_class, filterable=filterable) self.model_tag_association_class = model_tag_association_class # Tags cannot be sorted. self.sortable = False + # Column-specific attributes. self.tag_elt_id_gen = 0 + self.grid_name = grid_name def get_value( self, trans, grid, item ): self.tag_elt_id_gen += 1 elt_id="tagging-elt" + str( self.tag_elt_id_gen ) div_elt = "<div id=%s></div>" % elt_id - return div_elt + trans.fill_template( "/tagging_common.mako", trans=trans, tagged_item=item, + return div_elt + trans.fill_template( "/tagging_common.mako", trans=trans, tagged_item=item, elt_context=self.grid_name, elt_id = elt_id, in_form="true", input_size="20", tag_click_fn="add_tag_to_grid_filter" ) def filter( self, db_session, query, column_filter ): """ Modify query to filter model_class by tag. Multiple filters are ANDed. """ diff -r efed28fa6a5e -r e75b55cc9e08 templates/dataset/edit_attributes.mako --- a/templates/dataset/edit_attributes.mako Mon Nov 30 16:47:18 2009 -0500 +++ b/templates/dataset/edit_attributes.mako Tue Dec 01 09:53:36 2009 -0500 @@ -59,7 +59,7 @@ </div> <div style="clear: both"></div> </div> - ${render_tagging_element(data, "dataset-tag-area", use_toggle_link="false", in_form="true", input_size="30")} + ${render_tagging_element(data, "dataset-tag-area", "edit_attributes.mako", use_toggle_link="false", in_form="true", input_size="30")} %endif %for name, spec in data.metadata.spec.items(): %if spec.visible: diff -r efed28fa6a5e -r e75b55cc9e08 templates/history/view.mako --- a/templates/history/view.mako Mon Nov 30 16:47:18 2009 -0500 +++ b/templates/history/view.mako Tue Dec 01 09:53:36 2009 -0500 @@ -327,7 +327,7 @@ %if trans.get_user() is not None: <div id='history-tag-area' class="tag-element"></div> - ${render_tagging_element(history, "history-tag-area", use_toggle_link='false', get_toggle_link_text_fn='get_toggle_link_text', editable=user_owns_history)} + ${render_tagging_element(history, "history-tag-area", "history/view.mako", use_toggle_link='false', get_toggle_link_text_fn='get_toggle_link_text', editable=user_owns_history)} %endif %if not datasets: diff -r efed28fa6a5e -r e75b55cc9e08 templates/root/history.mako --- a/templates/root/history.mako Mon Nov 30 16:47:18 2009 -0500 +++ b/templates/root/history.mako Tue Dec 01 09:53:36 2009 -0500 @@ -325,7 +325,7 @@ %if trans.get_user() is not None: <div id='history-tag-area' class="tag-element"></div> - ${render_tagging_element(history, "history-tag-area", get_toggle_link_text_fn='get_toggle_link_text')} + ${render_tagging_element( tagged_item=history, elt_id="history-tag-area", elt_context="history.mako", get_toggle_link_text_fn='get_toggle_link_text' )} %endif %if not datasets: diff -r efed28fa6a5e -r e75b55cc9e08 templates/tagging_common.mako --- a/templates/tagging_common.mako Mon Nov 30 16:47:18 2009 -0500 +++ b/templates/tagging_common.mako Tue Dec 01 09:53:36 2009 -0500 @@ -1,10 +1,10 @@ ## Render a tagging element if there is a tagged_item. %if tagged_item is not None and elt_id is not None: - ${render_tagging_element(tagged_item, elt_id=elt_id, in_form=in_form, input_size=input_size, tag_click_fn=tag_click_fn)} + ${render_tagging_element(tagged_item=tagged_item, elt_id=elt_id, elt_context=elt_context, in_form=in_form, input_size=input_size, tag_click_fn=tag_click_fn)} %endif ## Render the tags 'tags' as an autocomplete element. -<%def name="render_tagging_element(tagged_item, elt_id, use_toggle_link='true', in_form='false', input_size='15', tag_click_fn='default_tag_click_fn', get_toggle_link_text_fn='default_get_toggle_link_text_fn', editable='true')"> +<%def name="render_tagging_element(tagged_item, elt_id, elt_context, use_toggle_link='true', in_form='false', input_size='15', tag_click_fn='default_tag_click_fn', get_toggle_link_text_fn='default_get_toggle_link_text_fn', editable='true')"> <script type="text/javascript"> // // Set up autocomplete tagger. @@ -75,8 +75,8 @@ tag_click_fn: ${tag_click_fn}, <% tagged_item_id = trans.security.encode_id(tagged_item.id) %> ajax_autocomplete_tag_url: "${h.url_for( controller='tag', action='tag_autocomplete_data', id=tagged_item_id, item_class=tagged_item.__class__.__name__ )}", - ajax_add_tag_url: "${h.url_for( controller='tag', action='add_tag_async', id=tagged_item_id, item_class=tagged_item.__class__.__name__ )}", - ajax_delete_tag_url: "${h.url_for( controller='tag', action='remove_tag_async', id=tagged_item_id, item_class=tagged_item.__class__.__name__ )}", + ajax_add_tag_url: "${h.url_for( controller='tag', action='add_tag_async', id=tagged_item_id, item_class=tagged_item.__class__.__name__, context=elt_context )}", + ajax_delete_tag_url: "${h.url_for( controller='tag', action='remove_tag_async', id=tagged_item_id, item_class=tagged_item.__class__.__name__, context=elt_context )}", delete_tag_img: "${h.url_for('/static/images/delete_tag_icon_gray.png')}", delete_tag_img_rollover: "${h.url_for('/static/images/delete_tag_icon_white.png')}", add_tag_img: "${h.url_for('/static/images/add_icon.png')}", diff -r efed28fa6a5e -r e75b55cc9e08 universe_wsgi.ini.sample --- a/universe_wsgi.ini.sample Mon Nov 30 16:47:18 2009 -0500 +++ b/universe_wsgi.ini.sample Tue Dec 01 09:53:36 2009 -0500 @@ -144,6 +144,9 @@ # Log events log_events = True +# Log user actions +log_actions = True + # Configuration for debugging middleware debug = True use_lint = False

1 0

[hg] galaxy 3134: Improved loading state for AJAXed grids: when ...
by Greg Von Kuster 02 Dec '09

02 Dec '09

details: http://www.bx.psu.edu/hg/galaxy/rev/6e742ffb16a5 changeset: 3134:6e742ffb16a5 user: jeremy goecks <jeremy.goecks at emory.edu> date: Tue Dec 01 11:07:20 2009 -0500 description: Improved loading state for AJAXed grids: when grid is loading, gray out table and prevent further user actions. diffstat: templates/grid_base.mako | 16 +++++++++++++++- 1 files changed, 15 insertions(+), 1 deletions(-) diffs (47 lines): diff -r e75b55cc9e08 -r 6e742ffb16a5 templates/grid_base.mako --- a/templates/grid_base.mako Tue Dec 01 09:53:36 2009 -0500 +++ b/templates/grid_base.mako Tue Dec 01 11:07:20 2009 -0500 @@ -601,6 +601,7 @@ // If there's an operation in the args, do POST; otherwise, do GET. var operation = url_args['operation']; var method = (operation != null && operation != undefined ? "POST" : "GET" ); + $('.loading-elt-overlay').show(); // Show overlay to indicate loading and prevent user actions. $.ajax({ type: method, url: "${h.url_for()}", @@ -660,6 +661,9 @@ } } + // Hide loading overlay. + $('.loading-elt-overlay').hide(); + // Show message if there is one. var message = $.trim( parsed_response_text[2] ); if (message != "") @@ -711,6 +715,15 @@ .gray-background { background-color: #DDDDDD; } + .loading-elt-overlay { + background-color : white; + opacity : 0.5; + width : 100%; + height : 85%; + z-index : 14000; + position : absolute; + display: none; + } </style> </%def> @@ -750,7 +763,8 @@ ## Render grid. <%def name="render_grid_table()"> <form action="${url()}" method="post" onsubmit="return false;"> - <table class="grid"> + <div class='loading-elt-overlay'></div> + <table id='grid-table' class="grid"> <thead id="grid-table-header"> <tr> <th></th>

1 0

[hg] galaxy 3128: When setting metadata externally, substitute a...
by Greg Von Kuster 02 Dec '09

02 Dec '09

details: http://www.bx.psu.edu/hg/galaxy/rev/92d6a9796b24 changeset: 3128:92d6a9796b24 user: Dan Blankenberg <dan(a)bx.psu.edu> date: Mon Nov 30 14:55:30 2009 -0500 description: When setting metadata externally, substitute a MetadataTempFile object for all populated metadata FileParameters. Fixes the problem with writting to metadata FileParameter files on cluster nodes with read-only database/files directory. diffstat: lib/galaxy/datatypes/metadata.py | 21 +++- lib/galaxy/model/mapping.py | 1 + lib/galaxy/model/migrate/versions/0028_external_metadata_file_override.py | 47 +++++++++ scripts/set_metadata.py | 23 ++++- tools/regVariation/quality_filter.xml | 2 +- 5 files changed, 87 insertions(+), 7 deletions(-) diffs (172 lines): diff -r 9f7a2a3be0e7 -r 92d6a9796b24 lib/galaxy/datatypes/metadata.py --- a/lib/galaxy/datatypes/metadata.py Mon Nov 30 11:59:54 2009 -0500 +++ b/lib/galaxy/datatypes/metadata.py Mon Nov 30 14:55:30 2009 -0500 @@ -429,7 +429,9 @@ if MetadataTempFile.is_JSONified_value( value ): value = MetadataTempFile.from_JSON( value ) if isinstance( value, MetadataTempFile ): - mf = self.new_file( dataset = parent, **value.kwds ) + mf = parent.metadata.get( self.spec.name, None) + if mf is None: + mf = self.new_file( dataset = parent, **value.kwds ) shutil.move( value.file_name, mf.file_name ) value = mf.id return value @@ -521,7 +523,7 @@ if dataset_path.false_path and dataset_path.real_path == metadata_files.dataset.file_name: return dataset_path.false_path return "" - return "%s,%s,%s,%s,%s" % ( metadata_files.filename_in, metadata_files.filename_kwds, metadata_files.filename_out, metadata_files.filename_results_code, __get_filename_override() ) + return "%s,%s,%s,%s,%s,%s" % ( metadata_files.filename_in, metadata_files.filename_kwds, metadata_files.filename_out, metadata_files.filename_results_code, __get_filename_override(), metadata_files.filename_override_metadata ) if not isinstance( datasets, list ): datasets = [ datasets ] if exec_dir is None: @@ -558,11 +560,22 @@ open( metadata_files.filename_out, 'wb+' ) # create the file on disk, so it cannot be reused by tempfile (unlikely, but possible) #file to store a 'return code' indicating the results of the set_meta() call #results code is like (True/False - if setting metadata was successful/failed , exception or string of reason of success/failure ) - metadata_files.filename_results_code = relpath( tempfile.NamedTemporaryFile( dir = tmp_dir, prefix = "metadata_out_%s_" % key ).name ) + metadata_files.filename_results_code = relpath( tempfile.NamedTemporaryFile( dir = tmp_dir, prefix = "metadata_results_%s_" % key ).name ) simplejson.dump( ( False, 'External set_meta() not called' ), open( metadata_files.filename_results_code, 'wb+' ) ) # create the file on disk, so it cannot be reused by tempfile (unlikely, but possible) #file to store kwds passed to set_meta() metadata_files.filename_kwds = relpath( tempfile.NamedTemporaryFile( dir = tmp_dir, prefix = "metadata_kwds_%s_" % key ).name ) simplejson.dump( kwds, open( metadata_files.filename_kwds, 'wb+' ), ensure_ascii=True ) + #existing metadata file parameters need to be overridden with cluster-writable file locations + metadata_files.filename_override_metadata = relpath( tempfile.NamedTemporaryFile( dir = tmp_dir, prefix = "metadata_override_%s_" % key ).name ) + open( metadata_files.filename_override_metadata, 'wb+' ) # create the file on disk, so it cannot be reused by tempfile (unlikely, but possible) + override_metadata = [] + for meta_key, spec_value in dataset.metadata.spec.iteritems(): + if isinstance( spec_value.param, FileParameter ) and dataset.metadata.get( meta_key, None ) is not None: + metadata_temp = MetadataTempFile() + shutil.copy( dataset.metadata.get( meta_key, None ).file_name, metadata_temp.file_name ) + override_metadata.append( ( meta_key, metadata_temp.to_JSON() ) ) + simplejson.dump( override_metadata, open( metadata_files.filename_override_metadata, 'wb+' ) ) + #add to session and flush sa_session.add( metadata_files ) sa_session.flush() metadata_files_list.append( metadata_files ) @@ -585,7 +598,7 @@ #can occur if the job was stopped before completion, but a MetadataTempFile is used in the set_meta MetadataTempFile.cleanup_from_JSON_dict_filename( metadata_files.filename_out ) dataset_key = self.get_dataset_metadata_key( metadata_files.dataset ) - for key, fname in [ ( 'filename_in', metadata_files.filename_in ), ( 'filename_out', metadata_files.filename_out ), ( 'filename_results_code', metadata_files.filename_results_code ), ( 'filename_kwds', metadata_files.filename_kwds ) ]: + for key, fname in [ ( 'filename_in', metadata_files.filename_in ), ( 'filename_out', metadata_files.filename_out ), ( 'filename_results_code', metadata_files.filename_results_code ), ( 'filename_kwds', metadata_files.filename_kwds ), ( 'filename_override_metadata', metadata_files.filename_override_metadata ) ]: try: os.remove( fname ) except Exception, e: diff -r 9f7a2a3be0e7 -r 92d6a9796b24 lib/galaxy/model/mapping.py --- a/lib/galaxy/model/mapping.py Mon Nov 30 11:59:54 2009 -0500 +++ b/lib/galaxy/model/mapping.py Mon Nov 30 14:55:30 2009 -0500 @@ -358,6 +358,7 @@ Column( "filename_out", String( 255 ) ), Column( "filename_results_code", String( 255 ) ), Column( "filename_kwds", String( 255 ) ), + Column( "filename_override_metadata", String( 255 ) ), Column( "job_runner_external_pid", String( 255 ) ) ) Event.table = Table( "event", metadata, diff -r 9f7a2a3be0e7 -r 92d6a9796b24 lib/galaxy/model/migrate/versions/0028_external_metadata_file_override.py --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/lib/galaxy/model/migrate/versions/0028_external_metadata_file_override.py Mon Nov 30 14:55:30 2009 -0500 @@ -0,0 +1,47 @@ +""" +This script adds the filename_override_metadata column to the JobExternalOutputMetadata table, +allowing existing metadata files to be written when using external metadata and a cluster +set up with read-only access to database/files +""" +from sqlalchemy import * +from sqlalchemy.orm import * +from sqlalchemy.exceptions import * +from migrate import * +from migrate.changeset import * +import datetime +now = datetime.datetime.utcnow +import sys, logging +# Need our custom types, but don't import anything else from model +from galaxy.model.custom_types import * + +log = logging.getLogger( __name__ ) +log.setLevel(logging.DEBUG) +handler = logging.StreamHandler( sys.stdout ) +format = "%(name)s %(levelname)s %(asctime)s %(message)s" +formatter = logging.Formatter( format ) +handler.setFormatter( formatter ) +log.addHandler( handler ) + +metadata = MetaData( migrate_engine ) +db_session = scoped_session( sessionmaker( bind=migrate_engine, autoflush=False, autocommit=True ) ) + +def display_migration_details(): + print "========================================" + print "This script adds the filename_override_metadata column to the JobExternalOutputMetadata table," + print" allowing existing metadata files to be written when using external metadata and a cluster" + print "set up with read-only access to database/files" + print "========================================" +def upgrade(): + display_migration_details() + # Load existing tables + metadata.reflect() + try: + job_external_output_metadata = Table( "job_external_output_metadata", metadata, autoload=True ) + col = Column( "filename_override_metadata", String( 255 ) ) + col.create( job_external_output_metadata ) + assert col is job_external_output_metadata.c.filename_override_metadata + except Exception, e: + log.debug( "Adding column 'filename_override_metadata' to job_external_output_metadata table failed: %s" % ( str( e ) ) ) + +def downgrade(): + pass diff -r 9f7a2a3be0e7 -r 92d6a9796b24 scripts/set_metadata.py --- a/scripts/set_metadata.py Mon Nov 30 11:59:54 2009 -0500 +++ b/scripts/set_metadata.py Mon Nov 30 14:55:30 2009 -0500 @@ -50,17 +50,36 @@ except: continue for filenames in sys.argv[1:]: - filename_in, filename_kwds, filename_out, filename_results_code, dataset_filename_override = filenames.split( ',' ) + fields = filenames.split( ',' ) + filename_in = fields.pop( 0 ) + filename_kwds = fields.pop( 0 ) + filename_out = fields.pop( 0 ) + filename_results_code = fields.pop( 0 ) + dataset_filename_override = fields.pop( 0 ) + #Need to be careful with the way that these parameters are populated from the filename splitting, + #because if a job is running when the server is updated, any existing external metadata command-lines + #will not have info about the newly added override_metadata file + if fields: + override_metadata = fields.pop( 0 ) + else: + override_metadata = None try: dataset = cPickle.load( open( filename_in ) ) #load DatasetInstance if dataset_filename_override: dataset.dataset.external_filename = dataset_filename_override if ext_override.get( dataset.dataset.id, None ): dataset.extension = ext_override[ dataset.dataset.id ] + #Metadata FileParameter types may not be writable on a cluster node, and are therefore temporarily substituted with MetadataTempFiles + if override_metadata: + override_metadata = simplejson.load( open( override_metadata ) ) + for metadata_name, metadata_file_override in override_metadata: + if galaxy.datatypes.metadata.MetadataTempFile.is_JSONified_value( metadata_file_override ): + metadata_file_override = galaxy.datatypes.metadata.MetadataTempFile.from_JSON( metadata_file_override ) + setattr( dataset.metadata, metadata_name, metadata_file_override ) kwds = stringify_dictionary_keys( simplejson.load( open( filename_kwds ) ) )#load kwds; need to ensure our keywords are not unicode dataset.datatype.set_meta( dataset, **kwds ) dataset.metadata.to_JSON_dict( filename_out ) # write out results of set_meta - simplejson.dump( ( True, 'Metadata has been set successfully' ), open( filename_results_code, 'wb+' ) ) #setting metadata has suceeded + simplejson.dump( ( True, 'Metadata has been set successfully' ), open( filename_results_code, 'wb+' ) ) #setting metadata has succeeded except Exception, e: simplejson.dump( ( False, str( e ) ), open( filename_results_code, 'wb+' ) ) #setting metadata has failed somehow clear_mappers() diff -r 9f7a2a3be0e7 -r 92d6a9796b24 tools/regVariation/quality_filter.xml --- a/tools/regVariation/quality_filter.xml Mon Nov 30 11:59:54 2009 -0500 +++ b/tools/regVariation/quality_filter.xml Mon Nov 30 14:55:30 2009 -0500 @@ -61,7 +61,7 @@ </conditional> </inputs> <outputs> - <data format="maf" name="out_file1"/> + <data format="maf" name="out_file1" metadata_source="input"/> </outputs> <requirements> <requirement type="python-module">numpy</requirement>

1 0

[hg] galaxy 3129: Fix MAF to BED tool. This tool should be rewri...
by Greg Von Kuster 02 Dec '09

02 Dec '09

details: http://www.bx.psu.edu/hg/galaxy/rev/9d4945bbdcf5 changeset: 3129:9d4945bbdcf5 user: Dan Blankenberg <dan(a)bx.psu.edu> date: Mon Nov 30 15:33:45 2009 -0500 description: Fix MAF to BED tool. This tool should be rewritten to not use a code file, but instead use one of the standard ways to create additional outputs, similar to how maf_to_interval functions. diffstat: tools/maf/maf_to_bed.py | 3 +-- tools/maf/maf_to_bed.xml | 4 ++-- tools/maf/maf_to_bed_code.py | 1 + 3 files changed, 4 insertions(+), 4 deletions(-) diffs (36 lines): diff -r 92d6a9796b24 -r 9d4945bbdcf5 tools/maf/maf_to_bed.py --- a/tools/maf/maf_to_bed.py Mon Nov 30 14:55:30 2009 -0500 +++ b/tools/maf/maf_to_bed.py Mon Nov 30 15:33:45 2009 -0500 @@ -15,8 +15,7 @@ input_filename = sys.argv[1] output_filename = sys.argv[2] #where to store files that become additional output - database_tmp_dir = './database/tmp' #os.path.join(os.path.split(os.path.split(os.path.realpath(output_filename))[0])[0],'tmp') #database/tmp - + database_tmp_dir = sys.argv[5] species = sys.argv[3].split(',') partial = sys.argv[4] diff -r 92d6a9796b24 -r 9d4945bbdcf5 tools/maf/maf_to_bed.xml --- a/tools/maf/maf_to_bed.xml Mon Nov 30 14:55:30 2009 -0500 +++ b/tools/maf/maf_to_bed.xml Mon Nov 30 15:33:45 2009 -0500 @@ -1,6 +1,6 @@ -<tool id="MAF_To_BED1" name="Maf to BED"> +<tool id="MAF_To_BED1" name="Maf to BED" force_history_refresh="True"> <description>Converts a MAF formated file to the BED format</description> - <command interpreter="python">maf_to_bed.py $input1 $out_file1 $species $complete_blocks</command> + <command interpreter="python">maf_to_bed.py $input1 $out_file1 $species $complete_blocks $__new_file_path__</command> <inputs> <param format="maf" name="input1" type="data" label="MAF file to convert"/> <param name="species" type="select" label="Select species" display="checkboxes" multiple="true" help="a separate history item will be created for each checked species"> diff -r 92d6a9796b24 -r 9d4945bbdcf5 tools/maf/maf_to_bed_code.py --- a/tools/maf/maf_to_bed_code.py Mon Nov 30 14:55:30 2009 -0500 +++ b/tools/maf/maf_to_bed_code.py Mon Nov 30 15:33:45 2009 -0500 @@ -46,6 +46,7 @@ newdata.state = newdata.states.ERROR newdata.dbkey = dbkey newdata.init_meta() + newdata.set_meta() newdata.set_peek() app.model.context.flush() output_data_list.append(newdata)

1 0

[hg] galaxy 3122: Request events & request rejection (resolves i...
by Greg Von Kuster 02 Dec '09

02 Dec '09

details: http://www.bx.psu.edu/hg/galaxy/rev/8d149264b777 changeset: 3122:8d149264b777 user: rc date: Wed Nov 25 14:06:28 2009 -0500 description: Request events & request rejection (resolves issue #221) - added request_events table - removed 'state' field in the request table - added 'new' & 'rejected' request states - color coded request states in the request grid Grids framework - added a confirm flag in grid operations which require confirmation before going through with operation diffstat: lib/galaxy/model/__init__.py | 35 +- lib/galaxy/model/mapping.py | 16 +- lib/galaxy/model/migrate/versions/0027_request_events.py | 94 ++++++ lib/galaxy/web/controllers/requests.py | 157 +++++++-- lib/galaxy/web/controllers/requests_admin.py | 299 +++++++++++++------ lib/galaxy/web/framework/helpers/grids.py | 4 +- templates/admin/requests/events.mako | 36 ++ templates/admin/requests/reject.mako | 42 ++ templates/admin/requests/show_request.mako | 13 +- templates/grid_base.mako | 6 +- templates/requests/events.mako | 39 ++ templates/requests/show_request.mako | 17 +- templates/sample/sample_events.mako | 43 +- test/base/twilltestcase.py | 9 +- test/functional/test_forms_and_requests.py | 30 +- 15 files changed, 651 insertions(+), 189 deletions(-) diffs (1296 lines): diff -r 5761948422a8 -r 8d149264b777 lib/galaxy/model/__init__.py --- a/lib/galaxy/model/__init__.py Tue Nov 24 14:53:19 2009 -0500 +++ b/lib/galaxy/model/__init__.py Wed Nov 25 14:06:28 2009 -0500 @@ -1260,11 +1260,12 @@ self.content = content class Request( object ): - states = Bunch( UNSUBMITTED = 'Unsubmitted', + states = Bunch( NEW = 'New', SUBMITTED = 'Submitted', + REJECTED = 'Rejected', COMPLETE = 'Complete') def __init__(self, name=None, desc=None, request_type=None, user=None, - form_values=None, library=None, folder=None, state=False): + form_values=None, library=None, folder=None): self.name = name self.desc = desc self.type = request_type @@ -1272,19 +1273,39 @@ self.user = user self.library = library self.folder = folder - self.state = state self.samples_list = [] + def state(self): + if self.events: + return self.events[0].state + return None + def last_comment(self): + if self.events: + if self.events[0].comment: + return self.events[0].comment + else: + return '' + return 'No comment' def has_sample(self, sample_name): for s in self.samples: if s.name == sample_name: return s return False + def unsubmitted(self): + return self.state() in [ self.states.REJECTED, self.states.NEW ] + def rejected(self): + return self.state() == self.states.REJECTED def submitted(self): - return self.state == self.states.SUBMITTED - def unsubmitted(self): - return self.state == self.states.UNSUBMITTED + return self.state() == self.states.SUBMITTED + def new(self): + return self.state() == self.states.NEW def complete(self): - return self.state == self.states.COMPLETE + return self.state() == self.states.COMPLETE + +class RequestEvent( object ): + def __init__(self, request=None, request_state=None, comment=''): + self.request = request + self.state = request_state + self.comment = comment class RequestType( object ): def __init__(self, name=None, desc=None, request_form=None, sample_form=None): diff -r 5761948422a8 -r 8d149264b777 lib/galaxy/model/mapping.py --- a/lib/galaxy/model/mapping.py Tue Nov 24 14:53:19 2009 -0500 +++ b/lib/galaxy/model/mapping.py Wed Nov 25 14:06:28 2009 -0500 @@ -618,8 +618,15 @@ Column( "user_id", Integer, ForeignKey( "galaxy_user.id" ), index=True ), Column( "library_id", Integer, ForeignKey( "library.id" ), index=True ), Column( "folder_id", Integer, ForeignKey( "library_folder.id" ), index=True ), + Column( "deleted", Boolean, index=True, default=False ) ) + +RequestEvent.table = Table('request_event', metadata, + Column( "id", Integer, primary_key=True), + Column( "create_time", DateTime, default=now ), + Column( "update_time", DateTime, default=now, onupdate=now ), + Column( "request_id", Integer, ForeignKey( "request.id" ), index=True ), Column( "state", TrimmedString( 255 ), index=True ), - Column( "deleted", Boolean, index=True, default=False ) ) + Column( "comment", TEXT ) ) Sample.table = Table('sample', metadata, Column( "id", Integer, primary_key=True ), @@ -768,9 +775,14 @@ folder=relation( LibraryFolder, primaryjoin=( Request.table.c.folder_id == LibraryFolder.table.c.id ) ), library=relation( Library, - primaryjoin=( Request.table.c.library_id == Library.table.c.id ) ) + primaryjoin=( Request.table.c.library_id == Library.table.c.id ) ), + events=relation( RequestEvent, backref="request", + order_by=desc(RequestEvent.table.c.update_time) ) ) ) +assign_mapper( context, RequestEvent, RequestEvent.table, + properties=None ) + assign_mapper( context, RequestType, RequestType.table, properties=dict( states=relation( SampleState, backref="request_type", diff -r 5761948422a8 -r 8d149264b777 lib/galaxy/model/migrate/versions/0027_request_events.py --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/lib/galaxy/model/migrate/versions/0027_request_events.py Wed Nov 25 14:06:28 2009 -0500 @@ -0,0 +1,94 @@ +""" +This migration script adds the request_event table and +removes the state field in the request table +""" +from sqlalchemy import * +from sqlalchemy.orm import * +from sqlalchemy.exc import * +from migrate import * +from migrate.changeset import * + +import datetime +now = datetime.datetime.utcnow + +import sys, logging +log = logging.getLogger( __name__ ) +log.setLevel(logging.DEBUG) +handler = logging.StreamHandler( sys.stdout ) +format = "%(name)s %(levelname)s %(asctime)s %(message)s" +formatter = logging.Formatter( format ) +handler.setFormatter( formatter ) +log.addHandler( handler ) + +# Need our custom types, but don't import anything else from model +from galaxy.model.custom_types import * + +metadata = MetaData( migrate_engine ) +db_session = scoped_session( sessionmaker( bind=migrate_engine, autoflush=False, autocommit=True ) ) + +def display_migration_details(): + print "========================================" + print "This migration script adds the request_event table and" + print "removes the state field in the request table" + print "========================================" + +def localtimestamp(): + if migrate_engine.name == 'postgres' or migrate_engine.name == 'mysql': + return "LOCALTIMESTAMP" + elif migrate_engine.name == 'sqlite': + return "current_date || ' ' || current_time" + else: + raise Exception( 'Unable to convert data for unknown database type: %s' % db ) + +def nextval( table, col='id' ): + if migrate_engine.name == 'postgres': + return "nextval('%s_%s_seq')" % ( table, col ) + elif migrate_engine.name == 'mysql' or migrate_engine.name == 'sqlite': + return "null" + else: + raise Exception( 'Unable to convert data for unknown database type: %s' % migrate_engine.name ) + + +RequestEvent_table = Table('request_event', metadata, + Column( "id", Integer, primary_key=True), + Column( "create_time", DateTime, default=now ), + Column( "update_time", DateTime, default=now, onupdate=now ), + Column( "request_id", Integer, ForeignKey( "request.id" ), index=True ), + Column( "state", TrimmedString( 255 ), index=True ), + Column( "comment", TEXT ) ) + +def upgrade(): + display_migration_details() + # Load existing tables + metadata.reflect() + # Add new request_event table + try: + RequestEvent_table.create() + except Exception, e: + log.debug( "Creating request_event table failed: %s" % str( e ) ) + # move the current state of all existing requests to the request_event table + cmd = \ + "INSERT INTO request_event " + \ + "SELECT %s AS id," + \ + "%s AS create_time," + \ + "%s AS update_time," + \ + "request.id AS request_id," + \ + "request.state AS state " + \ + "FROM request;" + cmd = cmd % ( nextval('request_event'), localtimestamp(), localtimestamp() ) + db_session.execute( cmd ) + + # Delete the state column + try: + Request_table = Table( "request", metadata, autoload=True ) + except NoSuchTableError: + Request_table = None + log.debug( "Failed loading table request" ) + if Request_table: + try: + Request_table.c.state.drop() + except Exception, e: + log.debug( "Deleting column 'state' to request table failed: %s" % ( str( e ) ) ) + +def downgrade(): + pass \ No newline at end of file diff -r 5761948422a8 -r 8d149264b777 lib/galaxy/web/controllers/requests.py --- a/lib/galaxy/web/controllers/requests.py Tue Nov 24 14:53:19 2009 -0500 +++ b/lib/galaxy/web/controllers/requests.py Wed Nov 25 14:06:28 2009 -0500 @@ -28,27 +28,57 @@ return request.type.name class LastUpdateColumn( grids.TextColumn ): def get_value(self, trans, grid, request): - return request.update_time + delta = datetime.utcnow() - request.update_time + if delta > timedelta( minutes=60 ): + last_update = '%s hours' % int( delta.seconds / 60 / 60 ) + else: + last_update = '%s minutes' % int( delta.seconds / 60 ) + return last_update class StateColumn( grids.GridColumn ): + def __init__( self, col_name, key, model_class, event_class, filterable, link ): + grids.GridColumn.__init__(self, col_name, key=key, model_class=model_class, filterable=filterable, link=link) + self.event_class = event_class + def get_value(self, trans, grid, request): + if request.state() == request.states.REJECTED: + return '<div class="count-box state-color-error">%s</div>' % request.state() + elif request.state() == request.states.NEW: + return '<div class="count-box state-color-queued">%s</div>' % request.state() + elif request.state() == request.states.SUBMITTED: + return '<div class="count-box state-color-running">%s</div>' % request.state() + elif request.state() == request.states.COMPLETE: + return '<div class="count-box state-color-ok">%s</div>' % request.state() + return request.state() def filter( self, db_session, query, column_filter ): """ Modify query to filter request by state. """ if column_filter == "All": return query if column_filter: - query = query.filter( model.Request.state == column_filter ) - return query + # select r.id, r.name, re.id, re.state + # from request as r, request_event as re + # where re.request_id=r.id and re.state='Complete' and re.create_time in + # (select MAX( create_time) + # from request_event + # group by request_id) + q = query.join(self.event_class.table)\ + .filter( self.model_class.table.c.id==self.event_class.table.c.request_id )\ + .filter( self.event_class.table.c.state==column_filter )\ + .filter( self.event_class.table.c.id.in_(select(columns=[func.max(self.event_class.table.c.id)], + from_obj=self.event_class.table, + group_by=self.event_class.table.c.request_id))) + return q def get_accepted_filters( self ): - """ Returns a list of accepted filters for this column. """ - accepted_filter_labels_and_vals = [ model.Request.states.UNSUBMITTED, - model.Request.states.SUBMITTED, - model.Request.states.COMPLETE, - "All"] - accepted_filters = [] - for val in accepted_filter_labels_and_vals: - label = val.lower() - args = { self.key: val } - accepted_filters.append( grids.GridColumnFilter( label, args) ) - return accepted_filters + """ Returns a list of accepted filters for this column. """ + accepted_filter_labels_and_vals = [ model.Request.states.NEW, + model.Request.states.REJECTED, + model.Request.states.SUBMITTED, + model.Request.states.COMPLETE, + "All"] + accepted_filters = [] + for val in accepted_filter_labels_and_vals: + label = val.lower() + args = { self.key: val } + accepted_filters.append( grids.GridColumnFilter( label, args) ) + return accepted_filters class DeletedColumn( grids.GridColumn ): def get_accepted_filters( self ): """ Returns a list of accepted filters for this column. """ @@ -66,7 +96,7 @@ num_rows_per_page = 50 preserve_state = True use_paging = True - default_filter = dict( deleted="False", state=model.Request.states.UNSUBMITTED) + default_filter = dict( deleted="False", state=model.Request.states.NEW) columns = [ NameColumn( "Name", key="name", @@ -83,23 +113,27 @@ TypeColumn( "Type" ), LastUpdateColumn( "Last update", format=time_ago ), - StateColumn( "State", - key='state', - filterable="advanced"), DeletedColumn( "Deleted", key="deleted", - visible=True, - filterable="advanced" ) + visible=False, + filterable="advanced" ), + StateColumn( "State", + model_class=model.Request, + event_class=model.RequestEvent, + key='state', + filterable="advanced", + link=( lambda item: iff( item.deleted, None, dict( operation="events", id=item.id ) ) ) ) ] columns.append( grids.MulticolFilterColumn( "Search", - cols_to_filter=[ columns[0], columns[1] ], + cols_to_filter=[ columns[0], columns[1], columns[6] ], key="free-text-search", visible=False, filterable="standard" ) ) operations = [ - grids.GridOperation( "Submit", allow_multiple=False, condition=( lambda item: not item.deleted and item.unsubmitted() and item.samples ) ), + grids.GridOperation( "Submit", allow_multiple=False, condition=( lambda item: not item.deleted and item.unsubmitted() and item.samples ), + confirm="More samples cannot be added to this request once it is submitted. Click OK to submit." ), grids.GridOperation( "Edit", allow_multiple=False, condition=( lambda item: not item.deleted and item.unsubmitted() ) ), - grids.GridOperation( "Delete", allow_multiple=True, condition=( lambda item: not item.deleted and item.unsubmitted() ) ), + grids.GridOperation( "Delete", allow_multiple=True, condition=( lambda item: not item.deleted and item.new() ) ), grids.GridOperation( "Undelete", allow_multiple=True, condition=( lambda item: item.deleted ) ) ] @@ -145,9 +179,23 @@ return self.__undelete_request( trans, **kwd ) elif operation == "edit": return self.__edit_request( trans, **kwd ) + elif operation == "events": + return self.__request_events( trans, **kwd ) + # if there are one or more requests that has been rejected by the admin + # recently, then show a msg as a reminder to the user + rlist = trans.sa_session.query( trans.app.model.Request ) \ + .filter( trans.app.model.Request.table.c.deleted==False ) \ + .filter( trans.app.model.Request.table.c.user_id==trans.user.id ) + rejected = 0 + for r in rlist: + if r.rejected(): + rejected = rejected + 1 + if rejected: + kwd['status'] = 'warning' + kwd['message'] = "%d requests (highlighted in red) were rejected, click on the request name for details." \ + % rejected # Render the list view return self.request_grid( trans, **kwd ) - def __show_request(self, trans, **kwd): params = util.Params( kwd ) msg = util.restore_text( params.get( 'msg', '' ) ) @@ -172,6 +220,28 @@ sample_copy=self.__copy_sample(current_samples), details='hide', edit_mode='False', msg=msg, messagetype=messagetype ) + def __request_events(self, trans, **kwd): + try: + request = trans.sa_session.query( trans.app.model.Request ).get( trans.security.decode_id(kwd['id']) ) + except: + msg = "Invalid request ID" + log.warn( msg ) + return trans.response.send_redirect( web.url_for( controller='requests', + action='list', + status='error', + message=msg, + **kwd) ) + events_list = [] + all_events = request.events + for event in all_events: + delta = datetime.utcnow() - event.update_time + if delta > timedelta( minutes=60 ): + last_update = '%s hours' % int( delta.seconds / 60 / 60 ) + else: + last_update = '%s minutes' % int( delta.seconds / 60 ) + events_list.append((event.state, last_update, event.comment)) + return trans.fill_template( '/requests/events.mako', + events_list=events_list, request=request) def request_details(self, trans, id): ''' Shows the request details @@ -187,7 +257,7 @@ value=request.type.name, helptext='')) request_details.append(dict(label='State', - value=request.state, + value=request.state(), helptext='')) request_details.append(dict(label='Date created', value=request.create_time, @@ -366,9 +436,9 @@ trans.sa_session.add( sample ) trans.sa_session.flush() return trans.response.send_redirect( web.url_for( controller='requests', - action='list', - operation='show_request', - id=trans.security.encode_id(request.id)) ) + action='list', + operation='show_request', + id=trans.security.encode_id(request.id)) ) elif params.get('edit_samples_button', False) == 'Edit samples': edit_mode = 'True' return trans.fill_template( '/requests/show_request.mako', @@ -700,10 +770,15 @@ if not request: request = trans.app.model.Request(name, desc, request_type, trans.user, form_values, - library=library, folder=folder, - state=trans.app.model.Request.states.UNSUBMITTED) + library=library, folder=folder) trans.sa_session.add( request ) trans.sa_session.flush() + trans.sa_session.refresh( request ) + # create an event with state 'New' for this new request + comments = "Request created." + event = trans.app.model.RequestEvent(request, request.states.NEW, comments) + trans.sa_session.add( event ) + trans.sa_session.flush() else: request.name = name request.desc = desc @@ -712,7 +787,6 @@ request.values = form_values request.library = library request.folder = folder - request.state = trans.app.model.Request.states.UNSUBMITTED trans.sa_session.add( request ) trans.sa_session.flush() return request @@ -731,7 +805,7 @@ message="Invalid request ID", **kwd) ) if params.get('show', False) == 'True': - return self.__edit_request(trans, **kwd) + return self.__edit_request(trans, id=trans.security.encode_id(request.id), **kwd) elif params.get('save_changes_request_button', False) == 'Save changes' \ or params.get('edit_samples_button', False) == 'Edit samples': request_type = trans.sa_session.query( trans.app.model.RequestType ).get( int( params.select_request_type ) ) @@ -760,7 +834,7 @@ messagetype='done', **new_kwd) ) elif params.get('refresh', False) == 'true': - return self.__edit_request(trans, **kwd) + return self.__edit_request(trans, id=trans.security.encode_id(request.id), **kwd) def __edit_request(self, trans, **kwd): try: @@ -819,7 +893,7 @@ message=msg, **kwd) ) # a request cannot be deleted once its submitted - if not request.unsubmitted(): + if not request.new(): delete_failed.append(request.name) else: request.deleted = True @@ -870,21 +944,22 @@ msg = self.__validate(trans, request) if msg: return trans.response.send_redirect( web.url_for( controller='requests', - action='edit', + action='list', + operation='edit', messagetype = 'error', msg=msg, - request_id=request.id, - show='True') ) + id=trans.security.encode_id(request.id) )) + # change the request state to 'Submitted' + comments = "Request moved to 'Submitted' state." + event = trans.app.model.RequestEvent(request, request.states.SUBMITTED, comments) + trans.sa_session.add( event ) + trans.sa_session.flush() # get the new state new_state = request.type.states[0] for s in request.samples: event = trans.app.model.SampleEvent(s, new_state, 'Samples submitted to the system') trans.sa_session.add( event ) trans.sa_session.flush() - # change request's submitted field - request.state = request.states.SUBMITTED - trans.sa_session.add( request ) - trans.sa_session.flush() return trans.response.send_redirect( web.url_for( controller='requests', action='list', id=trans.security.encode_id(request.id), diff -r 5761948422a8 -r 8d149264b777 lib/galaxy/web/controllers/requests_admin.py --- a/lib/galaxy/web/controllers/requests_admin.py Tue Nov 24 14:53:19 2009 -0500 +++ b/lib/galaxy/web/controllers/requests_admin.py Wed Nov 25 14:06:28 2009 -0500 @@ -8,6 +8,8 @@ from galaxy.web.form_builder import * from datetime import datetime, timedelta from galaxy.web.controllers.forms import get_all_forms +from sqlalchemy.sql.expression import func, and_ +from sqlalchemy.sql import select log = logging.getLogger( __name__ ) @@ -31,27 +33,58 @@ return request.type.name class LastUpdateColumn( grids.TextColumn ): def get_value(self, trans, grid, request): - return request.update_time + delta = datetime.utcnow() - request.update_time + if delta > timedelta( minutes=60 ): + last_update = '%s hours' % int( delta.seconds / 60 / 60 ) + else: + last_update = '%s minutes' % int( delta.seconds / 60 ) + return last_update class StateColumn( grids.GridColumn ): + def __init__( self, col_name, key, model_class, event_class, filterable, link ): + grids.GridColumn.__init__(self, col_name, key=key, model_class=model_class, filterable=filterable, link=link) + self.event_class = event_class + def get_value(self, trans, grid, request): + if request.state() == request.states.REJECTED: + return '<div class="count-box state-color-error">%s</div>' % request.state() + elif request.state() == request.states.NEW: + return '<div class="count-box state-color-queued">%s</div>' % request.state() + elif request.state() == request.states.SUBMITTED: + return '<div class="count-box state-color-running">%s</div>' % request.state() + elif request.state() == request.states.COMPLETE: + return '<div class="count-box state-color-ok">%s</div>' % request.state() + return request.state() def filter( self, db_session, query, column_filter ): """ Modify query to filter request by state. """ if column_filter == "All": return query if column_filter: - query = query.filter( model.Request.state == column_filter ) - return query + # select r.id, r.name, re.id, re.state + # from request as r, request_event as re + # where re.request_id=r.id and re.state='Complete' and re.create_time in + # (select MAX( create_time) + # from request_event + # group by request_id) + q = query.join(self.event_class.table)\ + .filter( self.model_class.table.c.id==self.event_class.table.c.request_id )\ + .filter( self.event_class.table.c.state==column_filter )\ + .filter( self.event_class.table.c.id.in_(select(columns=[func.max(self.event_class.table.c.id)], + from_obj=self.event_class.table, + group_by=self.event_class.table.c.request_id))) + #print column_filter, q + return q def get_accepted_filters( self ): - """ Returns a list of accepted filters for this column. """ - accepted_filter_labels_and_vals = [ model.Request.states.UNSUBMITTED, - model.Request.states.SUBMITTED, - model.Request.states.COMPLETE, - "All"] - accepted_filters = [] - for val in accepted_filter_labels_and_vals: - label = val.lower() - args = { self.key: val } - accepted_filters.append( grids.GridColumnFilter( label, args) ) - return accepted_filters + """ Returns a list of accepted filters for this column. """ + accepted_filter_labels_and_vals = [ model.Request.states.NEW, + model.Request.states.REJECTED, + model.Request.states.SUBMITTED, + model.Request.states.COMPLETE, + "All"] + accepted_filters = [] + for val in accepted_filter_labels_and_vals: + label = val.lower() + args = { self.key: val } + accepted_filters.append( grids.GridColumnFilter( label, args) ) + return accepted_filters class UserColumn( grids.TextColumn ): def get_value(self, trans, grid, request): return request.user.email @@ -86,20 +119,25 @@ filterable="advanced" ), SamplesColumn( "Sample(s)", link=( lambda item: iff( item.deleted, None, dict( operation="show_request", id=item.id ) ) ), ), - TypeColumn( "Type" ), + TypeColumn( "Type", + link=( lambda item: iff( item.deleted, None, dict( operation="view_type", id=item.type.id ) ) ), ), LastUpdateColumn( "Last update", format=time_ago ), - StateColumn( "State", - key='state', - filterable="advanced"), - UserColumn( "User", - key='user.email', - model_class=model.Request, - filterable="advanced" ), DeletedColumn( "Deleted", key="deleted", - visible=True, - filterable="advanced" ) + visible=False, + filterable="advanced" ), + StateColumn( "State", + model_class=model.Request, + event_class=model.RequestEvent, + key='state', + filterable="advanced", + link=( lambda item: iff( item.deleted, None, dict( operation="events", id=item.id ) ) ), + ), + UserColumn( "User", + #key='user.email', + model_class=model.Request) + ] columns.append( grids.MulticolFilterColumn( "Search", cols_to_filter=[ columns[0], columns[1], columns[6] ], @@ -107,10 +145,11 @@ visible=False, filterable="standard" ) ) operations = [ - grids.GridOperation( "Submit", allow_multiple=False, condition=( lambda item: not item.deleted and item.unsubmitted() and item.samples ) ), + grids.GridOperation( "Submit", allow_multiple=False, condition=( lambda item: not item.deleted and item.unsubmitted() and item.samples ), + confirm="More samples cannot be added to this request once it is submitted. Click OK to submit." ), grids.GridOperation( "Edit", allow_multiple=False, condition=( lambda item: not item.deleted ) ), grids.GridOperation( "Reject", allow_multiple=False, condition=( lambda item: not item.deleted and item.submitted() ) ), - grids.GridOperation( "Delete", allow_multiple=True, condition=( lambda item: not item.deleted and item.unsubmitted() ) ), + grids.GridOperation( "Delete", allow_multiple=True, condition=( lambda item: not item.deleted and item.new() ) ), grids.GridOperation( "Undelete", condition=( lambda item: item.deleted ) ), ] global_actions = [ @@ -227,6 +266,11 @@ return self.__edit_request( trans, **kwd ) elif operation == "reject": return self.__reject_request( trans, **kwd ) + elif operation == "events": + return self.__request_events( trans, **kwd ) + elif operation == "view_type": + return self.__view_request_type( trans, **kwd ) + # Render the grid view return self.request_grid( trans, **kwd ) def __show_request(self, trans, **kwd): @@ -253,7 +297,52 @@ sample_copy=self.__copy_sample(current_samples), details='hide', edit_mode='False', msg=msg, messagetype=messagetype ) - + @web.expose + @web.require_admin + def edit(self, trans, **kwd): + params = util.Params( kwd ) + msg = util.restore_text( params.get( 'msg', '' ) ) + messagetype = params.get( 'messagetype', 'done' ) + try: + request = trans.sa_session.query( trans.app.model.Request ).get( int( params.get( 'request_id', None ) ) ) + except: + return trans.response.send_redirect( web.url_for( controller='requests_admin', + action='list', + status='error', + message="Invalid request ID", + **kwd) ) + if params.get('show', False) == 'True': + return self.__edit_request(trans, id=trans.security.encode_id(request.id), **kwd) + elif params.get('save_changes_request_button', False) == 'Save changes' \ + or params.get('edit_samples_button', False) == 'Edit samples': + request_type = trans.sa_session.query( trans.app.model.RequestType ).get( int( params.select_request_type ) ) + if not util.restore_text(params.get('name', '')): + msg = 'Please enter the <b>Name</b> of the request' + kwd['messagetype'] = 'error' + kwd['msg'] = msg + kwd['show'] = 'True' + return trans.response.send_redirect( web.url_for( controller='requests_admin', + action='edit', + **kwd) ) + request = self.__save_request(trans, request, **kwd) + msg = 'The changes made to the request named %s has been saved' % request.name + if params.get('save_changes_request_button', False) == 'Save changes': + return trans.response.send_redirect( web.url_for( controller='requests_admin', + action='list', + message=msg , + status='done') ) + elif params.get('edit_samples_button', False) == 'Edit samples': + new_kwd = {} + new_kwd['request_id'] = request.id + new_kwd['edit_samples_button'] = 'Edit samples' + return trans.response.send_redirect( web.url_for( controller='requests_admin', + action='show_request', + msg=msg , + messagetype='done', + **new_kwd) ) + elif params.get('refresh', False) == 'true': + return self.__edit_request(trans, id=trans.security.encode_id(request.id), **kwd) + def __edit_request(self, trans, **kwd): try: request = trans.sa_session.query( trans.app.model.Request ).get( trans.security.decode_id(kwd['id']) ) @@ -311,7 +400,7 @@ message=msg, **kwd) ) # a request cannot be deleted once its submitted - if not request.unsubmitted(): + if not request.new(): delete_failed.append(request.name) else: request.deleted = True @@ -362,18 +451,24 @@ msg = self.__validate(trans, request) if msg: return trans.response.send_redirect( web.url_for( controller='requests_admin', - action='edit', + action='list', + operation='edit', messagetype = 'error', msg=msg, - request_id=request.id, - show='True') ) - # get the new state + id=trans.security.encode_id(request.id) ) ) + # change the request state to 'Submitted' + if request.user.email is not trans.user: + comments = "Request moved to 'Submitted' state by admin (%s) on behalf of %s." % (trans.user.email, request.user.email) + else: + comments = "" + event = trans.app.model.RequestEvent(request, request.states.SUBMITTED, comments) + trans.sa_session.add( event ) + trans.sa_session.flush() + # change the state of each of the samples of thus request new_state = request.type.states[0] for s in request.samples: event = trans.app.model.SampleEvent(s, new_state, 'Samples submitted to the system') trans.sa_session.add( event ) - # change request's submitted field - request.state = request.states.SUBMITTED trans.sa_session.add( request ) trans.sa_session.flush() return trans.response.send_redirect( web.url_for( controller='requests_admin', @@ -393,15 +488,64 @@ status='error', message=msg, **kwd) ) - # change request's submitted field - request.state = request.states.UNSUBMITTED - trans.sa_session.add( request ) + return trans.fill_template( '/admin/requests/reject.mako', + request=request) + @web.expose + @web.require_admin + def reject(self, trans, **kwd): + params = util.Params( kwd ) + if params.get('cancel_reject_button', False): + return trans.response.send_redirect( web.url_for( controller='requests_admin', + action='list', + operation='show_request', + id=kwd['id'])) + try: + request = trans.sa_session.query( trans.app.model.Request ).get( trans.security.decode_id(kwd['id']) ) + except: + msg = "Invalid request ID" + log.warn( msg ) + return trans.response.send_redirect( web.url_for( controller='requests_admin', + action='list', + status='error', + message=msg, + **kwd) ) + # validate + if not params.get('comment', ''): + return trans.fill_template( '/admin/requests/reject.mako', + request=request, messagetype='error', + msg='A comment is required for rejecting a request.') + # create an event with state 'Rejected' for this request + comments = util.restore_text( params.comment ) + event = trans.app.model.RequestEvent(request, request.states.REJECTED, comments) + trans.sa_session.add( event ) trans.sa_session.flush() return trans.response.send_redirect( web.url_for( controller='requests_admin', action='list', status='done', - message='The request <b>%s</b> is now unsubmitted.' % request.name - ) ) + message='Request <b>%s</b> has been rejected.' % request.name) ) + + def __request_events(self, trans, **kwd): + try: + request = trans.sa_session.query( trans.app.model.Request ).get( trans.security.decode_id(kwd['id']) ) + except: + msg = "Invalid request ID" + log.warn( msg ) + return trans.response.send_redirect( web.url_for( controller='requests_admin', + action='list', + status='error', + message=msg, + **kwd) ) + events_list = [] + all_events = request.events + for event in all_events: + delta = datetime.utcnow() - event.update_time + if delta > timedelta( minutes=60 ): + last_update = '%s hours' % int( delta.seconds / 60 / 60 ) + else: + last_update = '%s minutes' % int( delta.seconds / 60 ) + events_list.append((event.state, last_update, event.comment)) + return trans.fill_template( '/admin/requests/events.mako', + events_list=events_list, request=request) # #---- Request Creation ---------------------------------------------------------- # @@ -732,9 +876,18 @@ if not request: request = trans.app.model.Request(name, desc, request_type, user, form_values, - library=library, folder=folder, - state=trans.app.model.Request.states.UNSUBMITTED) + library=library, folder=folder) trans.sa_session.add( request ) + trans.sa_session.flush() + trans.sa_session.refresh( request ) + # create an event with state 'New' for this new request + if request.user.email is not trans.user: + comments = "Request created by admin (%s) on behalf of %s." % (trans.user.email, request.user.email) + else: + comments = "Request created." + event = trans.app.model.RequestEvent(request, request.states.NEW, comments) + trans.sa_session.add( event ) + trans.sa_session.flush() else: request.name = name request.desc = desc @@ -744,58 +897,14 @@ request.library = library request.folder = folder trans.sa_session.add( request ) - trans.sa_session.flush() + trans.sa_session.flush() + return request # -#---- Request Editing ---------------------------------------------------------- +#---- Request Page ---------------------------------------------------------- # - @web.expose - @web.require_admin - def edit(self, trans, **kwd): - params = util.Params( kwd ) - msg = util.restore_text( params.get( 'msg', '' ) ) - messagetype = params.get( 'messagetype', 'done' ) - try: - request = trans.sa_session.query( trans.app.model.Request ).get( int( params.get( 'request_id', None ) ) ) - except: - return trans.response.send_redirect( web.url_for( controller='requests_admin', - action='list', - status='error', - message="Invalid request ID", - **kwd) ) - if params.get('show', False) == 'True': - return self.__edit_request(trans, request.id, **kwd) - elif params.get('save_changes_request_button', False) == 'Save changes' \ - or params.get('edit_samples_button', False) == 'Edit samples': - request_type = trans.sa_session.query( trans.app.model.RequestType ).get( int( params.select_request_type ) ) - if not util.restore_text(params.get('name', '')): - msg = 'Please enter the <b>Name</b> of the request' - kwd['messagetype'] = 'error' - kwd['msg'] = msg - kwd['show'] = 'True' - return trans.response.send_redirect( web.url_for( controller='requests_admin', - action='edit', - **kwd) ) - request = self.__save_request(trans, request, **kwd) - msg = 'The changes made to the request named %s has been saved' % request.name - if params.get('save_changes_request_button', False) == 'Save changes': - return trans.response.send_redirect( web.url_for( controller='requests_admin', - action='list', - message=msg , - status='done') ) - elif params.get('edit_samples_button', False) == 'Edit samples': - new_kwd = {} - new_kwd['request_id'] = request.id - new_kwd['edit_samples_button'] = 'Edit samples' - return trans.response.send_redirect( web.url_for( controller='requests_admin', - action='show_request', - msg=msg , - messagetype='done', - **new_kwd) ) - elif params.get('refresh', False) == 'true': - return self.__edit_request(trans, request.id, **kwd) def __update_samples(self, request, **kwd): ''' This method retrieves all the user entered sample information and @@ -1013,7 +1122,7 @@ value=request.type.name, helptext='')) request_details.append(dict(label='State', - value=request.state, + value=request.state(), helptext='')) request_details.append(dict(label='Date created', value=request.create_time, @@ -1167,11 +1276,13 @@ if s.current_state().id != request.type.states[-1].id: complete = False if complete: - request.state = request.states.COMPLETE - else: - request.state = request.states.SUBMITTED - trans.sa_session.add( request ) - trans.sa_session.flush() + # change the request state to 'Complete' + comments = "All samples of this request are in the last sample state (%s)." % request.type.states[-1].name + event = trans.app.model.RequestEvent(request, request.states.COMPLETE, comments) + trans.sa_session.add( event ) + trans.sa_session.flush() +# trans.sa_session.add( request ) +# trans.sa_session.flush() def change_state(self, trans, sample): possible_states = sample.request.type.states curr_state = sample.current_state() diff -r 5761948422a8 -r 8d149264b777 lib/galaxy/web/framework/helpers/grids.py --- a/lib/galaxy/web/framework/helpers/grids.py Tue Nov 24 14:53:19 2009 -0500 +++ b/lib/galaxy/web/framework/helpers/grids.py Wed Nov 25 14:06:28 2009 -0500 @@ -402,7 +402,7 @@ return query.filter( complete_filter ) class GridOperation( object ): - def __init__( self, label, key=None, condition=None, allow_multiple=True, allow_popup=True, target=None, url_args=None, async_compatible=False ): + def __init__( self, label, key=None, condition=None, allow_multiple=True, allow_popup=True, target=None, url_args=None, async_compatible=False, confirm=None ): self.label = label self.key = key self.allow_multiple = allow_multiple @@ -411,6 +411,8 @@ self.target = target self.url_args = url_args self.async_compatible = async_compatible + # if 'confirm' is set, then ask before completing the operation + self.confirm = confirm def get_url_args( self, item ): if self.url_args: temp = dict( self.url_args ) diff -r 5761948422a8 -r 8d149264b777 templates/admin/requests/events.mako --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/templates/admin/requests/events.mako Wed Nov 25 14:06:28 2009 -0500 @@ -0,0 +1,36 @@ +<%inherit file="/base.mako"/> +<%namespace file="/message.mako" import="render_msg" /> + +<h2>History of Sequencing Request "${request.name}"</h2> +<ul class="manage-table-actions"> + <li> + <a class="action-button" href="${h.url_for( controller='requests_admin', action='list', operation='show_request', id=trans.security.encode_id(request.id) )}"> + <span>Browse this request</span></a> + </li> +</ul> +<h3>User: ${request.user.email}</h3> + +%if msg: + ${render_msg( msg, messagetype )} +%endif + +<div class="toolForm"> + <table class="grid"> + <thead> + <tr> + <th>State</th> + <th>Last Update</th> + <th>Comments</th> + </tr> + </thead> + <tbody> + %for state, updated, comments in events_list: + <tr class="libraryRow libraryOrFolderRow" id="libraryRow"> + <td><b><a>${state}</a></b></td> + <td><a>${updated}</a></td> + <td><a>${comments}</a></td> + </tr> + %endfor + </tbody> + </table> +</div> diff -r 5761948422a8 -r 8d149264b777 templates/admin/requests/reject.mako --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/templates/admin/requests/reject.mako Wed Nov 25 14:06:28 2009 -0500 @@ -0,0 +1,42 @@ +<%inherit file="/base.mako"/> +<%namespace file="/message.mako" import="render_msg" /> + +%if msg: + ${render_msg( msg, messagetype )} +%endif + +<h2>Reject Sequencing Request "${request.name}"</h2> +<ul class="manage-table-actions"> + <li> + <a class="action-button" href="${h.url_for( controller='requests_admin', action='list', operation='show_request', id=trans.security.encode_id(request.id) )}"> + <span>Browse this request</span></a> + </li> + <li> + <a class="action-button" href="${h.url_for( controller='requests_admin', action='list', operation='events', id=trans.security.encode_id(request.id) )}"> + <span>Events</span></a> + </li> +</ul> +<h3>User: ${request.user.email}</h3> + + + +<div class="toolForm"> + <div class="toolFormTitle">Reject request</div> + <form name="event" action="${h.url_for( controller='requests_admin', action='reject', id=trans.security.encode_id(request.id))}" method="post" > + <div class="form-row"> + Rejecting this request will move the request state to <b>Rejected</b>. + </div> + <div class="form-row"> + <label>Comments</label> + <textarea name="comment" rows="5" cols="40"></textarea> + <div class="toolParamHelp" style="clear: both;"> + Required + </div> + </div> + <div class="form-row"> + <input type="submit" name="reject_button" value="Reject"/> + <input type="submit" name="cancel_reject_button" value="Cancel"/> + </div> + </form> + </div> +</div> \ No newline at end of file diff -r 5761948422a8 -r 8d149264b777 templates/admin/requests/show_request.mako --- a/templates/admin/requests/show_request.mako Tue Nov 24 14:53:19 2009 -0500 +++ b/templates/admin/requests/show_request.mako Wed Nov 25 14:06:28 2009 -0500 @@ -5,11 +5,16 @@ ${render_msg( msg, messagetype )} %endif +%if request.rejected(): + ${render_msg( "Reason for rejection: "+request.last_comment(), "warning" )} +%endif + <div class="grid-header"> <h2>Sequencing Request "${request.name}"</h2> </div> <ul class="manage-table-actions"> + %if request.unsubmitted() and request.samples: <li> <a class="action-button" confirm="More samples cannot be added to this request once it is submitted. Click OK to submit." href="${h.url_for( controller='requests_admin', action='list', operation='Submit', id=trans.security.encode_id(request.id) )}"> @@ -28,6 +33,10 @@ <span>Bar codes</span></a> </li> %endif + <li> + <a class="action-button" href="${h.url_for( controller='requests_admin', action='list', operation='events', id=trans.security.encode_id(request.id) )}"> + <span>History</span></a> + </li> </ul> @@ -82,7 +91,7 @@ %if grid_index == 0: <td>${sample.bar_code}</td> <td> - %if sample.request.unsubmitted(): + %if sample.request.new(): Unsubmitted %else: <a href="${h.url_for( controller='requests_admin', action='show_events', sample_id=sample.id)}">${sample.current_state().name}</a> @@ -117,6 +126,8 @@ %else: <i>None</i> %endif + %elif rd['label'] == 'State': + <a href="${h.url_for( controller='requests_admin', action='list', operation='events', id=trans.security.encode_id(request.id) )}">${rd['value']}</a> %else: ${rd['value']} %endif diff -r 5761948422a8 -r 8d149264b777 templates/grid_base.mako --- a/templates/grid_base.mako Tue Nov 24 14:53:19 2009 -0500 +++ b/templates/grid_base.mako Wed Nov 25 14:06:28 2009 -0500 @@ -860,7 +860,11 @@ if operation.target: target = "target='" + operation.target + "'" %> - <a class="action-button" ${target} href="${ url( **operation.get_url_args( item ) ) }">${operation.label}</a> + %if operation.confirm: + <a class="action-button" ${target} confirm="${operation.confirm}" href="${ url( **operation.get_url_args( item ) ) }">${operation.label}</a> + %else: + <a class="action-button" ${target} href="${ url( **operation.get_url_args( item ) ) }">${operation.label}</a> + %endif %endif %endfor </div> diff -r 5761948422a8 -r 8d149264b777 templates/requests/events.mako --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/templates/requests/events.mako Wed Nov 25 14:06:28 2009 -0500 @@ -0,0 +1,39 @@ +<%inherit file="/base.mako"/> +<%namespace file="/message.mako" import="render_msg" /> + +<h2>History of Sequencing Request "${request.name}"</h2> +<ul class="manage-table-actions"> + <li> + <a class="action-button" href="${h.url_for( controller='requests', action='list', operation='show_request', id=trans.security.encode_id(request.id) )}"> + <span>Browse this request</span></a> + </li> + <li> + <a class="action-button" href="${h.url_for( controller='requests', action='list')}"> + <span>Browse all requests</span></a> + </li> +</ul> + +%if msg: + ${render_msg( msg, messagetype )} +%endif + +<div class="toolForm"> + <table class="grid"> + <thead> + <tr> + <th>State</th> + <th>Last Update</th> + <th>Comments</th> + </tr> + </thead> + <tbody> + %for state, updated, comments in events_list: + <tr class="libraryRow libraryOrFolderRow" id="libraryRow"> + <td><b><a>${state}</a></b></td> + <td><a>${updated}</a></td> + <td><a>${comments}</a></td> + </tr> + %endfor + </tbody> + </table> +</div> diff -r 5761948422a8 -r 8d149264b777 templates/requests/show_request.mako --- a/templates/requests/show_request.mako Tue Nov 24 14:53:19 2009 -0500 +++ b/templates/requests/show_request.mako Wed Nov 25 14:06:28 2009 -0500 @@ -6,6 +6,9 @@ ${render_msg( msg, messagetype )} %endif +%if request.rejected(): + ${render_msg( "Reason for rejection: "+request.last_comment(), "warning" )} +%endif <div class="grid-header"> <h2>Sequencing Request "${request.name}"</h2> @@ -19,6 +22,10 @@ </li> %endif <li> + <a class="action-button" href="${h.url_for( controller='requests', action='list', operation='events', id=trans.security.encode_id(request.id) )}"> + <span>History</span></a> + </li> + <li> <a class="action-button" href="${h.url_for( controller='requests', action='list')}"> <span>Browse requests</span></a> </li> @@ -73,7 +80,7 @@ </td> %if grid_index == 0: <td> - %if sample.request.unsubmitted(): + %if sample.request.new(): Unsubmitted %else: <a href="${h.url_for( controller='requests', action='show_events', sample_id=sample.id)}">${sample.current_state().name}</a> @@ -105,9 +112,11 @@ %if rd['label'] == 'Data library': %if rd['value']: <a href="${h.url_for( controller='library', action='browse_library', obj_id=request.library.id )}">${rd['value']}</a> - %else: - <i>None</i> - %endif + %elif rd['label'] == 'State': + <a href="${h.url_for( controller='requests', action='list', operation='events', id=trans.security.encode_id(request.id) )}">${rd['value']}</a> + %else: + <i>None</i> + %endif %else: ${rd['value']} %endif diff -r 5761948422a8 -r 8d149264b777 templates/sample/sample_events.mako --- a/templates/sample/sample_events.mako Tue Nov 24 14:53:19 2009 -0500 +++ b/templates/sample/sample_events.mako Wed Nov 25 14:06:28 2009 -0500 @@ -21,24 +21,25 @@ ${render_msg( msg, messagetype )} %endif - -<table class="grid"> - <thead> - <tr> - <th>State</th> - <th>Description</th> - <th>Updated</th> - <th>Comments</th> - </tr> - </thead> - <tbody> - %for state, desc, updated, comments in events_list: - <tr class="libraryRow libraryOrFolderRow" id="libraryRow"> - <td><b><a>${state}</a></b></td> - <td><a>${desc}</a></td> - <td><a>${updated}</a></td> - <td><a>${comments}</a></td> - </tr> - %endfor - </tbody> -</table> \ No newline at end of file +<div class="toolForm"> + <table class="grid"> + <thead> + <tr> + <th>State</th> + <th>Description</th> + <th>Updated</th> + <th>Comments</th> + </tr> + </thead> + <tbody> + %for state, desc, updated, comments in events_list: + <tr class="libraryRow libraryOrFolderRow" id="libraryRow"> + <td><b><a>${state}</a></b></td> + <td><a>${desc}</a></td> + <td><a>${updated}</a></td> + <td><a>${comments}</a></td> + </tr> + %endfor + </tbody> + </table> +</div> \ No newline at end of file diff -r 5761948422a8 -r 8d149264b777 test/base/twilltestcase.py --- a/test/base/twilltestcase.py Tue Nov 24 14:53:19 2009 -0500 +++ b/test/base/twilltestcase.py Wed Nov 25 14:06:28 2009 -0500 @@ -1298,10 +1298,15 @@ self.home() self.visit_url( "%s/requests_admin/list?operation=Submit&id=%s" % ( self.url, self.security.encode_id( request_id ) )) self.check_page_for_string( 'The request <b>%s</b> has been submitted.' % request_name ) - def reject_request( self, request_id, request_name ): + def reject_request( self, request_id, request_name, comment ): self.home() self.visit_url( "%s/requests_admin/list?operation=Reject&id=%s" % ( self.url, self.security.encode_id( request_id ) )) - self.check_page_for_string( 'The request <b>%s</b> is now unsubmitted.' % request_name ) + self.check_page_for_string( 'Reject Sequencing Request "%s"' % request_name ) + tc.fv( "1", "comment", comment ) + tc.submit( "reject_button" ) + self.check_page_for_string( 'Request <b>%s</b> has been rejected.' % request_name ) + self.visit_url( "%s/requests/list?sort=-create_time&operation=show_request&id=%s" % ( self.url, self.security.encode_id( request_id ) )) + self.check_page_for_string( comment ) def add_bar_codes( self, request_id, request_name, bar_codes ): self.home() self.visit_url( "%s/requests_admin/bar_codes?request_id=%i" % (self.url, request_id) ) diff -r 5761948422a8 -r 8d149264b777 test/functional/test_forms_and_requests.py --- a/test/functional/test_forms_and_requests.py Tue Nov 24 14:53:19 2009 -0500 +++ b/test/functional/test_forms_and_requests.py Wed Nov 25 14:06:28 2009 -0500 @@ -194,9 +194,9 @@ .filter( and_( galaxy.model.Request.table.c.name==request_name, galaxy.model.Request.table.c.deleted==False ) ) \ .first() - # check if the request's state is now set to 'unsubmitted' - assert request_one.state is not request_one.states.UNSUBMITTED, "The state of the request '%s' should be set to '%s'" \ - % ( request_one.name, request_one.states.UNSUBMITTED ) + # check if the request's state is now set to 'new' + assert request_one.state is not request_one.states.NEW, "The state of the request '%s' should be set to '%s'" \ + % ( request_one.name, request_one.states.NEW ) # sample fields samples = [ ( 'Sample One', [ 'S1 Field 0 Value' ] ), ( 'Sample Two', [ 'S2 Field 0 Value' ] ) ] @@ -207,8 +207,8 @@ self.edit_request(request_one.id, request_one.name, request_one.name+' (Renamed)', request_one.desc+' (Re-described)', library_one.id, folder_one.id, fields) sa_session.refresh( request_one ) - # check if the request is showing in the 'unsubmitted' filter - self.check_request_grid(state='Unsubmitted', request_name=request_one.name) + # check if the request is showing in the 'new' filter + self.check_request_grid(state='New', request_name=request_one.name) # submit the request self.submit_request( request_one.id, request_one.name ) sa_session.refresh( request_one ) @@ -257,11 +257,11 @@ .filter( and_( galaxy.model.Request.table.c.name==request_name, galaxy.model.Request.table.c.deleted==False ) ) \ .first() - # check if the request is showing in the 'unsubmitted' filter - self.check_request_admin_grid(state='Unsubmitted', request_name=request_two.name) - # check if the request's state is now set to 'unsubmitted' - assert request_two.state is not request_two.states.UNSUBMITTED, "The state of the request '%s' should be set to '%s'" \ - % ( request_two.name, request_two.states.UNSUBMITTED ) + # check if the request is showing in the 'new' filter + self.check_request_admin_grid(state='New', request_name=request_two.name) + # check if the request's state is now set to 'new' + assert request_two.state is not request_two.states.NEW, "The state of the request '%s' should be set to '%s'" \ + % ( request_two.name, request_two.states.NEW ) # sample fields samples = [ ( 'Sample One', [ 'S1 Field 0 Value' ] ), ( 'Sample Two', [ 'S2 Field 0 Value' ] ) ] @@ -282,10 +282,10 @@ '''Testing rejecting a request''' self.logout() self.login( email='test(a)bx.psu.edu' ) - self.reject_request( request_two.id, request_two.name ) + self.reject_request( request_two.id, request_two.name, "Rejection test comment" ) sa_session.refresh( request_two ) - # check if the request is showing in the 'unsubmitted' filter - self.check_request_admin_grid(state='Unsubmitted', request_name=request_two.name) + # check if the request is showing in the 'rejected' filter + self.check_request_admin_grid(state='Rejected', request_name=request_two.name) # check if the request's state is now set to 'submitted' - assert request_two.state is not request_two.states.UNSUBMITTED, "The state of the request '%s' should be set to '%s'" \ - % ( request_two.name, request_two.states.UNSUBMITTED ) + assert request_two.state is not request_two.states.REJECTED, "The state of the request '%s' should be set to '%s'" \ + % ( request_two.name, request_two.states.REJECTED )

1 0

[hg] galaxy 3123: First pass of the lastz tool wrapper that incl...
by Greg Von Kuster 02 Dec '09

02 Dec '09

details: http://www.bx.psu.edu/hg/galaxy/rev/efdb6fb5de34 changeset: 3123:efdb6fb5de34 user: Greg Von Kuster <greg(a)bx.psu.edu> date: Fri Nov 27 10:53:50 2009 -0500 description: First pass of the lastz tool wrapper that includes job splitting - works but needs polishing. diffstat: test-data/B1.fa | 1000 ---------------------------------------- test-data/b1.fasta | 1000 ++++++++++++++++++++++++++++++++++++++++ test-data/lastz_wrapper_out1.sam | 494 +++++++++++++++++++ test-data/lastz_wrapper_out3.tabular | 26 + test-data/phiX.fa | 79 --- test-data/phiX.fasta | 79 +++ tool-data/lastz_seqs.loc | 2 + tool_conf.xml.sample | 2 +- tools/sr_mapping/bwa_wrapper.xml | 2 +- tools/sr_mapping/lastz_wrapper.py | 133 +++++ tools/sr_mapping/lastz_wrapper.xml | 300 +++++++---- 11 files changed, 1913 insertions(+), 1204 deletions(-) diffs (truncated from 3185 to 3000 lines): diff -r 8d149264b777 -r efdb6fb5de34 test-data/B1.fa --- a/test-data/B1.fa Wed Nov 25 14:06:28 2009 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,1000 +0,0 @@ ->HWI-EAS91_1_306UPAAXX:6:1:1503:1160 -GGTGGTCTATAGTGTTATTAATATCAAGTTGGGGGG ->HWI-EAS91_1_306UPAAXX:6:1:1564:1179 -GCGAGCAGTAGACTCCTTCTGTTGATAAGCAAGCAT ->HWI-EAS91_1_306UPAAXX:6:1:1704:1082 -GATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTC ->HWI-EAS91_1_306UPAAXX:6:1:1588:1797 -GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT ->HWI-EAS91_1_306UPAAXX:6:1:1304:1526 -GTAGTTGAAATGGTAATAAGACGACCAATCTGACCT ->HWI-EAS91_1_306UPAAXX:6:1:1490:1582 -GTCGTGTTCAACAGACCTATAAACATTCTGTGCCGC ->HWI-EAS91_1_306UPAAXX:6:1:1356:1339 -GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC ->HWI-EAS91_1_306UPAAXX:6:1:1311:853 -GGTTGGTTTATCGTTTTTGACACTCTCACGTTGTCT ->HWI-EAS91_1_306UPAAXX:6:1:1257:1552 -GTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTC ->HWI-EAS91_1_306UPAAXX:6:1:1486:1402 -GTTACTGAGAAGTTAATGGATGAATTGGCACAATGC ->HWI-EAS91_1_306UPAAXX:6:1:1028:1081 -GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT ->HWI-EAS91_1_306UPAAXX:6:1:1167:752 -GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC ->HWI-EAS91_1_306UPAAXX:6:1:1507:1113 -GTCAACGTTATATTTTGATAGTTTGACGGTTAATTC ->HWI-EAS91_1_306UPAAXX:6:1:1654:1311 -GGATGAAAATGCTCACAATGACAAATCTGTCCACGG ->HWI-EAS91_1_306UPAAXX:6:1:1386:1060 -GTTCTTGGTCAGTATGCAAATTAGCATAAGCAGCTT ->HWI-EAS91_1_306UPAAXX:6:1:1070:1356 -GGTTACAGTATGCCCATCGCAGTTCGCTACACGCAG ->HWI-EAS91_1_306UPAAXX:6:1:787:1032 -GCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCA ->HWI-EAS91_1_306UPAAXX:6:1:834:1017 -GCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGT ->HWI-EAS91_1_306UPAAXX:6:1:1703:1155 -GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT ->HWI-EAS91_1_306UPAAXX:6:1:1406:593 -GTTGAGTTCGATAATGGTGATATGTATGTTTACGGC ->HWI-EAS91_1_306UPAAXX:6:1:1411:886 -GTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCAT ->HWI-EAS91_1_306UPAAXX:6:1:923:972 -GCATGACAAGTAAAGGACGGTTGTCAGCGTCATAAG ->HWI-EAS91_1_306UPAAXX:6:1:1279:1004 -GCCATAGCACCAGAAACAAAACTAGGGGCGGCCTCT ->HWI-EAS91_1_306UPAAXX:6:1:1070:840 -GGTTGTCAGCGTCATAAGAGGTTTTACCTCCAAATG ->HWI-EAS91_1_306UPAAXX:6:1:1595:1040 -GTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGT ->HWI-EAS91_1_306UPAAXX:6:1:1002:559 -GAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAG ->HWI-EAS91_1_306UPAAXX:6:1:999:974 -GTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGC ->HWI-EAS91_1_306UPAAXX:6:1:896:982 -GTGGCTGGAGACAAATAATCTCTTTAATAACCTTAT ->HWI-EAS91_1_306UPAAXX:6:1:1366:741 -GTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGC ->HWI-EAS91_1_306UPAAXX:6:1:749:1469 -GTTTATGGTGAACAGTGGATTAAGTTCATGAAGGAT ->HWI-EAS91_1_306UPAAXX:6:1:1010:592 -GAGTTTATTGCTGCCGTCATTGCTTATTATGTTCAT ->HWI-EAS91_1_306UPAAXX:6:1:1393:650 -GTGACTCATATCTAAACCAGTCCTTGACGAACGTGC ->HWI-EAS91_1_306UPAAXX:6:1:1238:1731 -GAGAAATAAAAGTCTGAAACATGATTAAACTCCTAA ->HWI-EAS91_1_306UPAAXX:6:1:1629:908 -GATGCGGTTATCCATCTGCTTATGGAAGCCAAGCAT ->HWI-EAS91_1_306UPAAXX:6:1:1560:849 -GCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAG ->HWI-EAS91_1_306UPAAXX:6:1:1029:783 -GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT ->HWI-EAS91_1_306UPAAXX:6:1:1152:1324 -GACAATCAGAAAGAGATTGCCGAGATGCAAAATGAG ->HWI-EAS91_1_306UPAAXX:6:1:1614:2042 -GAAATGCCACAAGCCTCAATAGCAGGTTTAAGAGCC ->HWI-EAS91_1_306UPAAXX:6:1:1398:439 -GATGGTTGGTTTATCGTTTTTGACACTCTCACGTTG ->HWI-EAS91_1_306UPAAXX:6:1:955:616 -GACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGC ->HWI-EAS91_1_306UPAAXX:6:1:1672:753 -GAATGCCAGCAATCTCTTTTTGAGTCTCATTTTGCT ->HWI-EAS91_1_306UPAAXX:6:1:1195:1293 -GCAATGCGACAGGCTCATGCTGATGGTTGGTTTATC ->HWI-EAS91_1_306UPAAXX:6:1:1074:755 -GCAAGAGTAAACATAGTGCCATGCTCAGGAACAAAG ->HWI-EAS91_1_306UPAAXX:6:1:984:499 -GACTTAGTTCATCAGCAAACGCAGAATCAGCGGTAT ->HWI-EAS91_1_306UPAAXX:6:1:1452:1833 -GCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGT ->HWI-EAS91_1_306UPAAXX:6:1:863:710 -GAGTTCGATAATGGTGATATGTATGTTGACGGCCAT ->HWI-EAS91_1_306UPAAXX:6:1:885:649 -GCAGAAGTTAACACTTTCGGATATTTCTGATGAGTC ->HWI-EAS91_1_306UPAAXX:6:1:917:1214 -GACAGATGTATCCATCTGAATGCAATGAAGAAAACC ->HWI-EAS91_1_306UPAAXX:6:1:892:1254 -GCTCAGGAAATGCAGCAGCAAGATAATCACGAGTAT ->HWI-EAS91_1_306UPAAXX:6:1:1555:1005 -GCATTTGGCGCATAATCTCGGAAACCTGCTGTTGCT ->HWI-EAS91_1_306UPAAXX:6:1:1637:1413 -GATGCTGTTCAACCACTAATAGGTAAGAAATCATGT ->HWI-EAS91_1_306UPAAXX:6:1:1102:1567 -GGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAA ->HWI-EAS91_1_306UPAAXX:6:1:799:1337 -GTATATGCACAAAATGAGATGCTTGCTTATCAACAG ->HWI-EAS91_1_306UPAAXX:6:1:1353:1843 -GCAGACCCATAATGTCAATAGATGTGGTAGAAGTCG ->HWI-EAS91_1_306UPAAXX:6:1:1196:789 -GCGGCATACGCTCGGCGCCAGTTTGAATATTAGACA ->HWI-EAS91_1_306UPAAXX:6:1:1056:1676 -GTAAAATACTGACCAGCCGTTTGAGCTTGAGTAAGC ->HWI-EAS91_1_306UPAAXX:6:1:1349:1836 -GGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTC ->HWI-EAS91_1_306UPAAXX:6:1:1027:788 -GGTGTTAATGCCACTCCTCTCCCGACTGTTAACACT ->HWI-EAS91_1_306UPAAXX:6:1:990:1283 -GCTTAGGGATTTTATTGGTATCAGGGTTAATCGTGC ->HWI-EAS91_1_306UPAAXX:6:1:904:939 -GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT ->HWI-EAS91_1_306UPAAXX:6:1:1732:793 -GTCAACATACATATCACCATTATCGAACTCAACGCC ->HWI-EAS91_1_306UPAAXX:6:1:1355:2003 -GTTAGACCAAACCATGAAACCAACATAAACATTATT ->HWI-EAS91_1_306UPAAXX:6:1:1337:977 -GCACCAGAAACAAAACTAGGGGCGGCCTCATCAGGG ->HWI-EAS91_1_306UPAAXX:6:1:1605:1175 -GGAGGTAAAACCTCTTATGACGCTGACAACCGTCCT ->HWI-EAS91_1_306UPAAXX:6:1:1763:1192 -GACAGGCCGTTTGAATGTTGACGGGATGAACATAAT ->HWI-EAS91_1_306UPAAXX:6:1:722:483 -GTTATTATACCGTCAAGGACTGTGTGACTATTGACT ->HWI-EAS91_1_306UPAAXX:6:1:1760:1136 -GCAAAGCATTGGGATTATCATAAAACGCCTCTAATC ->HWI-EAS91_1_306UPAAXX:6:1:1088:798 -GGAAACCTGCTGTTGCTTGGAAAGATTGGTGTTTTC ->HWI-EAS91_1_306UPAAXX:6:1:633:1076 -GCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAG ->HWI-EAS91_1_306UPAAXX:6:1:673:754 -GTCATGGAAGCGATAAAACTCTGCAGGTTGGATATT ->HWI-EAS91_1_306UPAAXX:6:1:1759:2019 -GTAAAGGACGGTTGTCAGCGTCATAAGAGGTTTTAC ->HWI-EAS91_1_306UPAAXX:6:1:1064:1797 -GCGGTTATCCATCTGCTTATGGAAGCCAAGCATTGG ->HWI-EAS91_1_306UPAAXX:6:1:1112:1669 -GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACT ->HWI-EAS91_1_306UPAAXX:6:1:510:1447 -GCATTAAGCTCAGGAAATGCAGCAGCAAGATAATCA ->HWI-EAS91_1_306UPAAXX:6:1:877:1573 -GTGCTATTGCTGGCGGTATTTCTTCTTCTTTTTTTT ->HWI-EAS91_1_306UPAAXX:6:1:870:542 -GAATGTCACGCTGATTATTTTGACTTTGAGCGTATC ->HWI-EAS91_1_306UPAAXX:6:1:966:384 -GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA ->HWI-EAS91_1_306UPAAXX:6:1:1186:1903 -GCCAGCGATAACCGGAGTAGTTGAAATGGTAATAAG ->HWI-EAS91_1_306UPAAXX:6:1:1632:1742 -GCATCACCCATGCCTACAGTATTGTTATCGGTAGCC ->HWI-EAS91_1_306UPAAXX:6:1:1521:559 -GAGAGCGCCAACGGCGTCCATCTCGAAGGAGTCGCC ->HWI-EAS91_1_306UPAAXX:6:1:683:454 -GCTTATTATGTTCATCCCGTCAACATTCAAACGTCC ->HWI-EAS91_1_306UPAAXX:6:1:112:1280 -GTTGGCGCTCTCCGTCTTTCTCCATTTCGTCGTGTC ->HWI-EAS91_1_306UPAAXX:6:1:891:381 -GACCAGGGCGAGCGCCAGAACGTTTTTTACCTTTAG ->HWI-EAS91_1_306UPAAXX:6:1:1348:958 -GATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTT ->HWI-EAS91_1_306UPAAXX:6:1:1785:1915 -GCCCCGAAGGGGACNANAAATGGTTTTTAGAGAACG ->HWI-EAS91_1_306UPAAXX:6:1:1418:42 -GTATGCCCATCGCAGTTCGCTACACGCAGGACGCTT ->HWI-EAS91_1_306UPAAXX:6:1:1421:743 -GGTCAACGCTACCTGTAGGAAGTGTCCGCATAAAAT ->HWI-EAS91_1_306UPAAXX:6:1:1079:790 -GCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGT ->HWI-EAS91_1_306UPAAXX:6:1:663:740 -GGTATTAAGGATGAGTGTTCAAGATTGCTGGATGCC ->HWI-EAS91_1_306UPAAXX:6:1:1245:413 -GTTTGAATGTTGACGGGATGAACATAATAAGCAATG ->HWI-EAS91_1_306UPAAXX:6:1:1378:1035 -GCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGG ->HWI-EAS91_1_306UPAAXX:6:1:903:1746 -GTACGGGGAAGGACGTCAATAGTCACACAGTCCTTG ->HWI-EAS91_1_306UPAAXX:6:1:1713:1134 -GGCGTACGGGGAAGGACGTCAATAGTCACACAGTCC ->HWI-EAS91_1_306UPAAXX:6:1:1246:1887 -GCTCTAATCTCTGGGCATCTGGCTATGATGTTGATG ->HWI-EAS91_1_306UPAAXX:6:1:872:1731 -GGGCGGCCTCATCAGGGTTAGGAACATTAGAGCCTT ->HWI-EAS91_1_306UPAAXX:6:1:1714:1582 -GCTTTCCTGCTCCTGTTGAGTTTATTGCTTCCGTCT ->HWI-EAS91_1_306UPAAXX:6:1:1785:763 -GNCGAGAAATAAAANNNTGAAACATGATTAAANTCC ->HWI-EAS91_1_306UPAAXX:6:1:1684:542 -GAAAAGACAGAATCTCTTCCAAGAGCTTGATGCGGT ->HWI-EAS91_1_306UPAAXX:6:1:1581:1665 -GACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATT ->HWI-EAS91_1_306UPAAXX:6:1:901:1581 -GTGCTGATATTGCTTTTGATGCCGACCCTAAATTTT ->HWI-EAS91_1_306UPAAXX:6:1:1128:239 -GGTTATTATACCGTCAAGGACTGTGTGACTATTGAC ->HWI-EAS91_1_306UPAAXX:6:1:969:441 -GGTAAGAAATCATGAGTCAAGTTACTGAACAATCCG ->HWI-EAS91_1_306UPAAXX:6:1:630:1087 -GCCACCATGATTATGACCAGTGTTTCCAGTCCGTTC ->HWI-EAS91_1_306UPAAXX:6:1:606:1852 -GGAGACAAATAATCTCTTTAATAACCTGATTCAGCG ->HWI-EAS91_1_306UPAAXX:6:1:489:1315 -GAAAGCTCAGTCTCAGGAGGAAGCGGAGCAGTCCAC ->HWI-EAS91_1_306UPAAXX:6:1:465:1983 -GAGCCAATACCATCAGCTTTACCGTCTTTCCAGAAA ->HWI-EAS91_1_306UPAAXX:6:1:559:1028 -GAGTGCTTAATCCAACTTACCAAGCTGGGTTACGAC ->HWI-EAS91_1_306UPAAXX:6:1:1655:1413 -GTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCG ->HWI-EAS91_1_306UPAAXX:6:1:980:605 -GCCGTTTGAATGTTGACGGGATGAACATAATAAGCA ->HWI-EAS91_1_306UPAAXX:6:1:1629:1865 -GAAAAGCGGCATGGTCAATATAACCAGTAGTGTTAT ->HWI-EAS91_1_306UPAAXX:6:1:1180:1920 -GCACTCCGTGGACAGATTTGTCATTGTGAGCATTTT ->HWI-EAS91_1_306UPAAXX:6:1:1116:383 -GCGCAGGAAACACTGACGTTCTTACTGACGCAGAAG ->HWI-EAS91_1_306UPAAXX:6:1:906:2041 -GTCACGTTTATGGTGAACAGTGGATTAAGTTCATGA ->HWI-EAS91_1_306UPAAXX:6:1:1514:157 -GTCAATAGATGTGGTAGAAGTCGTCATTTGGCGTGG ->HWI-EAS91_1_306UPAAXX:6:1:1032:1857 -GCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGT ->HWI-EAS91_1_306UPAAXX:6:1:638:609 -GATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAT ->HWI-EAS91_1_306UPAAXX:6:1:74:750 -GATAATCACGAGTATCCTTTCCTTTATCATCTTCAT ->HWI-EAS91_1_306UPAAXX:6:1:486:822 -GTTGACGATGTAGCTTTAGGTGTCTTTAAAACAGGT ->HWI-EAS91_1_306UPAAXX:6:1:899:473 -GAACAGCATCGGACTCAGATAGTAATCCACGCTCTT ->HWI-EAS91_1_306UPAAXX:6:1:1613:197 -GTGACATTCAGAAGGGTAATAAGAACGAACCATAAA ->HWI-EAS91_1_306UPAAXX:6:1:326:1747 -GTTGAGGCTTTCGTTTATTGTACGCTTTGCTTTTTT ->HWI-EAS91_1_306UPAAXX:6:1:1487:526 -GCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT ->HWI-EAS91_1_306UPAAXX:6:1:629:665 -GAAATGCAGCAGCAAGATAATCACGAGTATCCTTTC ->HWI-EAS91_1_306UPAAXX:6:1:766:744 -GGCCGTCAACATACATATCACCATTATCGAACTCAA ->HWI-EAS91_1_306UPAAXX:6:1:391:1771 -GTGGTTGATATTTTTCATGGTATTGATAAATCTTTT ->HWI-EAS91_1_306UPAAXX:6:1:591:1102 -GCTTTGCGTGACTATTTTCGTGATATTGTTCGTTTG ->HWI-EAS91_1_306UPAAXX:6:1:917:664 -GCCATGATGGTGGTTATTATACCGTCAAGGACTGTG ->HWI-EAS91_1_306UPAAXX:6:1:217:737 -GTTCAGTTGTTGCATTGGAATATTCAGTTTAAATTT ->HWI-EAS91_1_306UPAAXX:6:1:1047:839 -GACCATTCAAAGGATAAACATCATAGGCAGTCGGGG ->HWI-EAS91_1_306UPAAXX:6:1:558:1040 -GCCACCAGCAAGAGCAGAAGCAATACCGCCAGCAAT ->HWI-EAS91_1_306UPAAXX:6:1:1207:524 -GCCAATACCATCAGCTTTACCGTCTTTCCAGAAATT ->HWI-EAS91_1_306UPAAXX:6:1:708:1634 -GCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAG ->HWI-EAS91_1_306UPAAXX:6:1:576:1851 -GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAG ->HWI-EAS91_1_306UPAAXX:6:1:906:460 -GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC ->HWI-EAS91_1_306UPAAXX:6:1:693:1260 -GCGAAAGGTCGCAAAGTAAGAGCTTCTCGAGCTGCG ->HWI-EAS91_1_306UPAAXX:6:1:1373:286 -GGACACTTCCTACAGGTAGCGTTGACCCTAATTTTG ->HWI-EAS91_1_306UPAAXX:6:1:762:41 -GATACTTGGAACAATTTCTGGAAAGACGGTAAAGCT ->HWI-EAS91_1_306UPAAXX:6:1:475:1091 -GTCACACAGTCCTTGACGGTATAATAACCACCATCT ->HWI-EAS91_1_306UPAAXX:6:1:791:627 -GCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACC ->HWI-EAS91_1_306UPAAXX:6:1:336:1791 -GAAGGAGTCGCCAGCGATAACCGGAGTAGTTGAAAT ->HWI-EAS91_1_306UPAAXX:6:1:1483:943 -GCACGTAATTTTTGACGCACGTTTTCTTCTGCGTCT ->HWI-EAS91_1_306UPAAXX:6:1:641:1071 -GATGGGCATACTGTAACCATAAGGCCACGTATTTTG ->HWI-EAS91_1_306UPAAXX:6:1:196:755 -GAACGCCCTCTTAAGGATATTCGCGATGAGTATAAT ->HWI-EAS91_1_306UPAAXX:6:1:463:1398 -GTCATAAGAGGTTTTACCTCCAAATGAAGAAATAAC ->HWI-EAS91_1_306UPAAXX:6:1:1559:460 -GCTCACAATGACAAATCTGTCCACGGAGTGCTTAAT ->HWI-EAS91_1_306UPAAXX:6:1:1625:1561 -GAGGAGTGGCATTAACACCATCCTTCATGAACTTAC ->HWI-EAS91_1_306UPAAXX:6:1:1729:1588 -GCTGATAAAGGAAAGGATACTCGTGATTATCTTGCT ->HWI-EAS91_1_306UPAAXX:6:1:945:393 -GGCCTCATCAGGGTTAGGAACATTAGAGCCTTGAAT ->HWI-EAS91_1_306UPAAXX:6:1:298:1391 -GTAAAGTTAGACCAAACCATGAAACCAACATAAACA ->HWI-EAS91_1_306UPAAXX:6:1:1270:1500 -GAATTACTACTGCTTGTTTACGAATTAAATATATGT ->HWI-EAS91_1_306UPAAXX:6:1:481:1546 -GCTGGCATTCAGTCGGCGACTTCACGCCAGAATACG ->HWI-EAS91_1_306UPAAXX:6:1:473:1729 -GTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAT ->HWI-EAS91_1_306UPAAXX:6:1:801:1831 -GCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAAT ->HWI-EAS91_1_306UPAAXX:6:1:536:639 -GCCGACCCTAAATTTTTTGCCTGTTTGGTTCTCTTT ->HWI-EAS91_1_306UPAAXX:6:1:259:938 -GTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTG ->HWI-EAS91_1_306UPAAXX:6:1:907:1513 -GGCATGGGTGATGCTGGTATTAAATCTGCCATTCAC ->HWI-EAS91_1_306UPAAXX:6:1:372:1409 -GATGAGTATAATTACCCCAAAAAGAAAGGTATTAAG ->HWI-EAS91_1_306UPAAXX:6:1:485:1626 -GATGGCAGCAACGGAAACCATAACGAGCATCATCTT ->HWI-EAS91_1_306UPAAXX:6:1:583:1679 -GCTCAAAGTCAAAATAATCAGCGTGACATTCAGAAG ->HWI-EAS91_1_306UPAAXX:6:1:690:1610 -GACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTT ->HWI-EAS91_1_306UPAAXX:6:1:257:918 -GCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTTCT ->HWI-EAS91_1_306UPAAXX:6:1:818:33 -GTGTTAATGCCACTCCTCTCCCGACTGTTAACTCTG ->HWI-EAS91_1_306UPAAXX:6:1:541:1242 -GGGATTATCATAAAACGCCTCTAATCGGTCGTCAGC ->HWI-EAS91_1_306UPAAXX:6:1:1014:279 -GTAAAAATGTCTACAGTAGAGTCAATAGCAAGGCCC ->HWI-EAS91_1_306UPAAXX:6:1:672:1790 -GGCCGTTTGAATGTTGACGGGATGAACATAATAAGC ->HWI-EAS91_1_306UPAAXX:6:1:708:464 -GGAGACAAATAATCTCTTTAATAACCTGATTCAGCG ->HWI-EAS91_1_306UPAAXX:6:1:633:1486 -GGGAAAGGTCATGCGGCATACGCTCGGCGCCAGTTT ->HWI-EAS91_1_306UPAAXX:6:1:328:696 -GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT ->HWI-EAS91_1_306UPAAXX:6:1:259:1389 -GCGTACTTATTCGCCACCATGATTATTACCAGTGTT ->HWI-EAS91_1_306UPAAXX:6:1:1315:41 -GCTTTCCGTGATGTCACAGCCTGCTTTGATGTGTCG ->HWI-EAS91_1_306UPAAXX:6:1:1647:549 -GCTTAATCCAACTTACCAAGCTGGGTTACGACGCGC ->HWI-EAS91_1_306UPAAXX:6:1:300:886 -GTTCTTGGTCAGTATGCAAATTAGCATAAGCAGCTT ->HWI-EAS91_1_306UPAAXX:6:1:317:1411 -GTACGCTGTACTTTGTGGGATACCCTCGCTTTCCTT ->HWI-EAS91_1_306UPAAXX:6:1:321:1819 -GGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGT ->HWI-EAS91_1_306UPAAXX:6:1:631:70 -GTGGATTACTATCTGAGTCCGATGCTGTTCAACCAC ->HWI-EAS91_1_306UPAAXX:6:1:624:1040 -GCTGGCGACTCCTTCGAGATGGACGCCGTTTGCGCT ->HWI-EAS91_1_306UPAAXX:6:1:662:1187 -GGGAGAGGAGTGGCATTAACACCATCCTTCATGACC ->HWI-EAS91_1_306UPAAXX:6:1:1440:1959 -GAATCAGCGGTATGGCTCCTCTCCTATTTTTGCTTC ->HWI-EAS91_1_306UPAAXX:6:1:458:1629 -GCTGGTGGCGCCATGTCTAAATTTTTTGGAGGCGGT ->HWI-EAS91_1_306UPAAXX:6:1:216:790 -GGGATGAAAATGCTCACAATGACAAATCTGTCCACG ->HWI-EAS91_1_306UPAAXX:6:1:1407:1174 -TTACCTATTAGTGGTTGAACAGCATCGGACTCAGAT ->HWI-EAS91_1_306UPAAXX:6:1:999:1790 -GTCCTGCGTGTAGCGAACTGCGATGGGCATACTGTC ->HWI-EAS91_1_306UPAAXX:6:1:141:1994 -GGCTTTTTTATGGTTCGTTCTTATTACCCTTCTTAT ->HWI-EAS91_1_306UPAAXX:6:1:225:465 -GTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGC ->HWI-EAS91_1_306UPAAXX:6:1:649:1760 -GACCCATAATGTCAATAGATGTGGTAGAAGTCGTCT ->HWI-EAS91_1_306UPAAXX:6:1:300:986 -GTTGAACACGACCAGAAAACTGGCCTAACGACGTTT ->HWI-EAS91_1_306UPAAXX:6:1:478:605 -GAGACTGAGCTTTCTCGCCAAATGACGACTTCTACC ->HWI-EAS91_1_306UPAAXX:6:1:622:395 -GGTAGCTTTAAGCGGCTCACCTTTAGCATCAACAGG ->HWI-EAS91_1_306UPAAXX:6:1:1701:574 -GTAAAGCCTCTACGCGATTTCATAGTGGAGGCCTCC ->HWI-EAS91_1_306UPAAXX:6:1:646:59 -GGAAGTGTCCGCATAAAATGCACCGCATGGAAATGT ->HWI-EAS91_1_306UPAAXX:6:1:284:2031 -GACAGAATCGTTAGTTGATGGCGAAAGGTCGCAAAG ->HWI-EAS91_1_306UPAAXX:6:1:22:1009 -GATGGATACATCTGTCAACGCCGCTAATCAGGTTGT ->HWI-EAS91_1_306UPAAXX:6:1:47:1826 -GCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCG ->HWI-EAS91_1_306UPAAXX:6:1:1025:1236 -TGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGT ->HWI-EAS91_1_306UPAAXX:6:1:773:591 -GAGCAGGAAAGCGAGGGTATCCCACAAAGTCCAGCG ->HWI-EAS91_1_306UPAAXX:6:1:1753:527 -GGTGGCATTCAAGGTGATGTGCTTGCTACCGATAAC ->HWI-EAS91_1_306UPAAXX:6:1:426:1717 -GTAGCGCCAATATGAGAAGAGCCATACCGCTGATTC ->HWI-EAS91_1_306UPAAXX:6:1:959:818 -TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC ->HWI-EAS91_1_306UPAAXX:6:1:459:1344 -GCCTATGATGTTTATCCTTTGAATGGTCGCCATGAT ->HWI-EAS91_1_306UPAAXX:6:1:973:1367 -TTCGTGATGAGTTTGTATCTGTTACTGATAAGTTAT ->HWI-EAS91_1_306UPAAXX:6:1:201:871 -GATTAGAGGCGTTTTATGATAATCCCAATGCTTTTC ->HWI-EAS91_1_306UPAAXX:6:1:713:1672 -GGCGTACGGGGAAGGACGTCAATAGTCACACAGTCC ->HWI-EAS91_1_306UPAAXX:6:1:444:1435 -TTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTGTG ->HWI-EAS91_1_306UPAAXX:6:1:288:1136 -GCCTTCCATGATGAGACAGGCCGTTTTAATTTTTTC ->HWI-EAS91_1_306UPAAXX:6:1:1653:225 -GCAAGGCCACGACGCAATGGAGAAAGACGGAGAGCG ->HWI-EAS91_1_306UPAAXX:6:1:537:1764 -GCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAA ->HWI-EAS91_1_306UPAAXX:6:1:196:1854 -GTATCGAGGCTCTTAAACCTGCTATTTAGGCTTTTT ->HWI-EAS91_1_306UPAAXX:6:1:312:1707 -GCGTCATAAGAGGTTTTACCTCCAAATGAAGAAATA ->HWI-EAS91_1_306UPAAXX:6:1:651:183 -GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT ->HWI-EAS91_1_306UPAAXX:6:1:295:694 -GTGATTACTTCATGCAGCGTTACCGTGATGTTATTT ->HWI-EAS91_1_306UPAAXX:6:1:330:1895 -GCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTG ->HWI-EAS91_1_306UPAAXX:6:1:590:331 -GAAATTTCTATGAATGATGTTTTCCGTTCTGGTGAT ->HWI-EAS91_1_306UPAAXX:6:1:481:1687 -GCAGATTGCGATAAACGGTCACATTAAATTTAACCT ->HWI-EAS91_1_306UPAAXX:6:1:1112:1279 -TGTGCATATACCTGGTCTTTCGTATTCTGTCGTGAT ->HWI-EAS91_1_306UPAAXX:6:1:1099:1216 -TTAGAGCGCATGACAAGTAAAGGACGGTTGTCAGCG ->HWI-EAS91_1_306UPAAXX:6:1:221:1238 -GTATCCTTTCCTTTATCATCGGCAGACTTTTCACCT ->HWI-EAS91_1_306UPAAXX:6:1:1015:364 -GCCAGCGATAACCGGAGTAGTTGAAATGGTAATAAG ->HWI-EAS91_1_306UPAAXX:6:1:735:1806 -TGTTATTAATATCAAGTTGGGGGAGCACATTGTAGC ->HWI-EAS91_1_306UPAAXX:6:1:320:411 -GCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAGTG ->HWI-EAS91_1_306UPAAXX:6:1:1273:1031 -TTAAGGATATTCGCGATGAGTATAATTACCCCAAAA ->HWI-EAS91_1_306UPAAXX:6:1:1456:1088 -AATAATCAGCGTGACATTCAGAAGGGTAATAAGAAC ->HWI-EAS91_1_306UPAAXX:6:1:1365:307 -GACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAG ->HWI-EAS91_1_306UPAAXX:6:1:478:252 -GATGCGGTTATCCATCTGCTTATTGAAGCCAAGCAT ->HWI-EAS91_1_306UPAAXX:6:1:915:1232 -TATTAATAACACTATAGACCACCGCCCCGAAGGGGC ->HWI-EAS91_1_306UPAAXX:6:1:680:1357 -TTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGC ->HWI-EAS91_1_306UPAAXX:6:1:238:1279 -GCCGAAGCCCCTGCAATTAAAATTGTTGACCACCTA ->HWI-EAS91_1_306UPAAXX:6:1:1583:35 -GCAAATTAGCATAAGCAGCTTGCAGACCCATAATGT ->HWI-EAS91_1_306UPAAXX:6:1:502:283 -GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT ->HWI-EAS91_1_306UPAAXX:6:1:418:1730 -GAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGT ->HWI-EAS91_1_306UPAAXX:6:1:596:647 -GCCTCAACGCAGCGACGAGCACGAGAGCGGTCAGTA ->HWI-EAS91_1_306UPAAXX:6:1:92:1591 -GTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCC ->HWI-EAS91_1_306UPAAXX:6:1:430:1938 -GCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGTCC ->HWI-EAS91_1_306UPAAXX:6:1:212:527 -GGTATTGATAAAGCTGTTGCCGATACTTGTAACAAT ->HWI-EAS91_1_306UPAAXX:6:1:594:942 -GACGACATTAGAAATATCCTTTGCAGTAGCGCCAAT ->HWI-EAS91_1_306UPAAXX:6:1:169:1774 -GCCTTCCATGATGAGACAGGCCGTTTTAATTTTTAC ->HWI-EAS91_1_306UPAAXX:6:1:1090:210 -GGAGAGCGCCAACGGCGTCCATCTCGAAGGAGTCGC ->HWI-EAS91_1_306UPAAXX:6:1:589:96 -GGCGGCCCCATCAGGGTTAGGAACATTAGAGCCTTG ->HWI-EAS91_1_306UPAAXX:6:1:1477:1231 -TAGGAACATTAGAGCCTTGAATGGCAGATTTAATAC ->HWI-EAS91_1_306UPAAXX:6:1:707:1076 -TCTGACGTTCGTGATGAGTTTGTATCTTTTTCTTTG ->HWI-EAS91_1_306UPAAXX:6:1:749:1715 -GAACATAATAAGCAATGACGGCAGCAATAAACTCAA ->HWI-EAS91_1_306UPAAXX:6:1:1738:1884 -GCTCACCTTTAGCATCAACAGGCCACAACCAACCAG ->HWI-EAS91_1_306UPAAXX:6:1:1160:1088 -TCACATTTTGTTCATGGTAGAGATTCTCTTGTTGAC ->HWI-EAS91_1_306UPAAXX:6:1:517:119 -GCAAGGCTAATGATTCACACGCCGACTGCTATCAGT ->HWI-EAS91_1_306UPAAXX:6:1:1472:716 -TGGTAATGGTGGTTTTCTTCATTTCATTCAGTTTTT ->HWI-EAS91_1_306UPAAXX:6:1:281:441 -GAGCAGTAGACTCCTTCTGTTGATAAGCAAGCATCT ->HWI-EAS91_1_306UPAAXX:6:1:1101:324 -AATACCATCAGCTTTACCGTCTTTCCAGAAATTGTT ->HWI-EAS91_1_306UPAAXX:6:1:1225:1494 -TTCTCAAATCCGGCGTCAACCATACCAGCAGAGGAA ->HWI-EAS91_1_306UPAAXX:6:1:1509:1025 -TTCTTGCTGCCGAGGGTCGCAAGGCTATTGTTTCAC ->HWI-EAS91_1_306UPAAXX:6:1:592:510 -GATACCAATAAAATCCCTAAGCATTTGTTTCTGGTT ->HWI-EAS91_1_306UPAAXX:6:1:324:1729 -GAACAAAGAAACGCGGCACAGAATGTTTATAGGTCT ->HWI-EAS91_1_306UPAAXX:6:1:128:1925 -GGAACAACTCACTAAAAACCAAGCTGTCGCTACTTC ->HWI-EAS91_1_306UPAAXX:6:1:786:893 -TACGGGGAAGGACGTCAATAGTCACACAGTCCTTGC ->HWI-EAS91_1_306UPAAXX:6:1:248:955 -GCTACAATGTGCTCCCCCAACTTGATATTAATAACA ->HWI-EAS91_1_306UPAAXX:6:1:388:1127 -GATATTGGTCGTATGGTTCTTGCTGCCTAGTGTCTC ->HWI-EAS91_1_306UPAAXX:6:1:721:1156 -TCTGGTTGGTTGTGGCCTTTTTATGCTAAATGTTAG ->HWI-EAS91_1_306UPAAXX:6:1:1564:1468 -TTACTTTTTATGTCCCTCATCGTCACGTTTATGTTG ->HWI-EAS91_1_306UPAAXX:6:1:750:77 -GGCTCATTCTGATTCTGAACAGCTTCTTGGGAAGTA ->HWI-EAS91_1_306UPAAXX:6:1:405:487 -GTTGGATTAAGCACTCCGTGGACAGATTTGTCATTT ->HWI-EAS91_1_306UPAAXX:6:1:836:1204 -TTGCTTCTGCTCTTGCTTGTGGCGCCATGTCTAAAT ->HWI-EAS91_1_306UPAAXX:6:1:224:1548 -GCTGCCGTCATTGCTTATTATGTTCATCCCTTCAAC ->HWI-EAS91_1_306UPAAXX:6:1:931:1015 -TTAAGGTACTGAATCTCTTTAGTCGCAGTAGGCGGT ->HWI-EAS91_1_306UPAAXX:6:1:329:579 -GTCCCTCATCGTCACGTTTATGGTGAACAGTGGATT ->HWI-EAS91_1_306UPAAXX:6:1:260:1145 -GCTTGCGTTTATGGTACGCTGGACTTTTTGTGATAC ->HWI-EAS91_1_306UPAAXX:6:1:1523:1253 -TTGGTAAAATACTGACCAGCCGTTTGAGCTTGAGTA ->HWI-EAS91_1_306UPAAXX:6:1:326:1271 -GACCACTCGCGATTCAATCATGACTTCGTGATAAAT ->HWI-EAS91_1_306UPAAXX:6:1:213:622 -GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA ->HWI-EAS91_1_306UPAAXX:6:1:274:712 -GCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTG ->HWI-EAS91_1_306UPAAXX:6:1:1549:627 -TATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATGT ->HWI-EAS91_1_306UPAAXX:6:1:1714:737 -TCTTTCGTATTCTGGCGTGAAGTCGCCGACTGAATG ->HWI-EAS91_1_306UPAAXX:6:1:760:1217 -TACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTT ->HWI-EAS91_1_306UPAAXX:6:1:174:768 -GTTGGCTGACGACCGATTAGAGGCGTTTTTTTATAT ->HWI-EAS91_1_306UPAAXX:6:1:172:1412 -GGTCGGCAGATTGCGATAAACGTTCACATTAAATTT ->HWI-EAS91_1_306UPAAXX:6:1:1393:869 -TTCATCCCGTCAACATTCAAACGGCCTGTCTCATCT ->HWI-EAS91_1_306UPAAXX:6:1:301:481 -GTTATAGATATTCAAATAACCCTGAAACAAATGCTT ->HWI-EAS91_1_306UPAAXX:6:1:648:1093 -TAACGCTGCATGAAGTAATCACGTTCTTGGTCAGTT ->HWI-EAS91_1_306UPAAXX:6:1:1233:591 -TTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCT ->HWI-EAS91_1_306UPAAXX:6:1:540:1415 -TTATTAAAGAGATTATTTTTCTCCAGCCACTTATGT ->HWI-EAS91_1_306UPAAXX:6:1:151:1792 -GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA ->HWI-EAS91_1_306UPAAXX:6:1:748:1378 -TGGATTACTATCTGAGTCCGATGCTGTTCAACCACT ->HWI-EAS91_1_306UPAAXX:6:1:1526:1479 -TGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCC ->HWI-EAS91_1_306UPAAXX:6:1:985:1093 -TAACCGTCTTCTCGTTCTCTAAAAACCATTTTTCTT ->HWI-EAS91_1_306UPAAXX:6:1:480:1378 -TCAACCTCAGCACTAACCTTGCGAGTCATTTCTTTG ->HWI-EAS91_1_306UPAAXX:6:1:903:753 -TGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAG ->HWI-EAS91_1_306UPAAXX:6:1:1697:1737 -GGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGT ->HWI-EAS91_1_306UPAAXX:6:1:803:1037 -TGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCTACT ->HWI-EAS91_1_306UPAAXX:6:1:1727:1244 -TTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCT ->HWI-EAS91_1_306UPAAXX:6:1:253:1162 -GCATTTAGTAGCGGTAAAGTTTGACCAAACCATTAT ->HWI-EAS91_1_306UPAAXX:6:1:216:856 -GTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCT ->HWI-EAS91_1_306UPAAXX:6:1:825:886 -TCCCACAAAGTCCAGCGTACCATAAACGCAAGCCTC ->HWI-EAS91_1_306UPAAXX:6:1:1699:962 -TGATTTCGATTTTCTGACGAGTAACAAAGTTTGGAT ->HWI-EAS91_1_306UPAAXX:6:1:1210:625 -TCAGATAGTAATCCACGCTCTTTTAAAATGTCAACA ->HWI-EAS91_1_306UPAAXX:6:1:538:616 -TAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGT ->HWI-EAS91_1_306UPAAXX:6:1:184:1849 -GCTCACCTTTAGCATCAACAGGCCACAACCAACCAG ->HWI-EAS91_1_306UPAAXX:6:1:1636:1103 -TATCTGACTTTTTGTTAACGTATTTAGCCACATAGA ->HWI-EAS91_1_306UPAAXX:6:1:605:223 -GGTTATTTGAATATCTATAACAACTATTTTAAATCG ->HWI-EAS91_1_306UPAAXX:6:1:256:1052 -GGTAAAGGACTTCTTGAAGGTACGTTGCAGTCTGGC ->HWI-EAS91_1_306UPAAXX:6:1:300:1515 -GCCATGATGGTGGTTATTATACCGTCAAGGACTTTT ->HWI-EAS91_1_306UPAAXX:6:1:1684:1320 -TGCTTGGCTTCCATAAGCAGATGGATAACCGCATCA ->HWI-EAS91_1_306UPAAXX:6:1:1186:895 -TCAGATGGATACATCTGTCAACGCCGCTAATCAGGT ->HWI-EAS91_1_306UPAAXX:6:1:1463:754 -TCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTT ->HWI-EAS91_1_306UPAAXX:6:1:808:1053 -TGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGG ->HWI-EAS91_1_306UPAAXX:6:1:960:1218 -TTTCTAATGTCGTCACTGATGCTGCTTCTGTTGTTT ->HWI-EAS91_1_306UPAAXX:6:1:521:1646 -GGAAAACGAACAAGCGCAAGAGTAAACATAGTGCCA ->HWI-EAS91_1_306UPAAXX:6:1:289:1885 -GCCAGCGATAACCGGAGTAGTTGAAATGGTAATAAG ->HWI-EAS91_1_306UPAAXX:6:1:471:170 -GGTCAGTTCCATCAACATCATAGCCAGATGCCCAGA ->HWI-EAS91_1_306UPAAXX:6:1:828:754 -TTTGCGTGACTATTTTCGTGATATTGTTCGTATGGT ->HWI-EAS91_1_306UPAAXX:6:1:924:1679 -TTTAATGTGACCGTTTATCGCAATCTGCCGACCACT ->HWI-EAS91_1_306UPAAXX:6:1:837:901 -TGCATTTTAGTAAGCTCTTTTTGATTCTCAAATCCG ->HWI-EAS91_1_306UPAAXX:6:1:543:16 -GCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTC ->HWI-EAS91_1_306UPAAXX:6:1:1482:578 -TCTTTAGCTCCTAGACCTTTAGCAGCAAGGTCCATA ->HWI-EAS91_1_306UPAAXX:6:1:1254:1668 -TTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCT ->HWI-EAS91_1_306UPAAXX:6:1:1402:898 -TCATGAGTCAAGTTACTGAACAATCCGTACGTTTCC ->HWI-EAS91_1_306UPAAXX:6:1:764:1534 -TTATACCGTCAAGGACTGTGTGACTATTGACGTCCT ->HWI-EAS91_1_306UPAAXX:6:1:681:1079 -TGGCGAATAAGTACGCGTTCTTGCAAATCACCAGAA ->HWI-EAS91_1_306UPAAXX:6:1:672:1350 -TTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTT ->HWI-EAS91_1_306UPAAXX:6:1:1266:493 -TGACCAGCCGTTTGAGCTTGAGTAAGCATTTGGCGC ->HWI-EAS91_1_306UPAAXX:6:1:118:238 -GACGGTATAATAACCACCATCATGGCGACCATTCAA ->HWI-EAS91_1_306UPAAXX:6:1:699:433 -TTATTGCCCGGCGTACGGGGAAGGACGTCAATAGTC ->HWI-EAS91_1_306UPAAXX:6:1:708:1387 -TGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTAC ->HWI-EAS91_1_306UPAAXX:6:1:498:1085 -TTATGATAATCCCAATGCTTTGCGTGACTATTTTCT ->HWI-EAS91_1_306UPAAXX:6:1:1101:1301 -TCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAAT ->HWI-EAS91_1_306UPAAXX:6:1:261:213 -GAATGGTCGCCATGATGGTGGTTATTATACCGTCAC ->HWI-EAS91_1_306UPAAXX:6:1:1287:1267 -TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGT ->HWI-EAS91_1_306UPAAXX:6:1:744:331 -TTAATGGATGAATTGGCACAATGCTACAATGTGCTC ->HWI-EAS91_1_306UPAAXX:6:1:614:814 -TGTCAGCGTCATAAGAGGTTTTACCTCCAAATGAAG ->HWI-EAS91_1_306UPAAXX:6:1:1362:1063 -TAAACGCAAGCCTCAACGCAGCGACGAGCACGAGAG ->HWI-EAS91_1_306UPAAXX:6:1:1238:1508 -TCAACTAACGATTCTGTCAAAAACTGACGCGTTGGT ->HWI-EAS91_1_306UPAAXX:6:1:904:1130 -TTATCGCAATCTGCCGACCACTCGCGATTCAATCAT ->HWI-EAS91_1_306UPAAXX:6:1:465:216 -GACCATGCCGCTTTTCTTGGCACGATTAACCCTTAT ->HWI-EAS91_1_306UPAAXX:6:1:844:628 -TAATGTCAATAGATGTGGTAGAAGTCGTCATTTGGC ->HWI-EAS91_1_306UPAAXX:6:1:684:1444 -TATCCCACAAAGTCCAGCGTACCATAAACGCAAGCC ->HWI-EAS91_1_306UPAAXX:6:1:515:1373 -TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCT ->HWI-EAS91_1_306UPAAXX:6:1:764:1667 -TTGAGTTCGATAATGGTGATATGTATGTTGACGTCC ->HWI-EAS91_1_306UPAAXX:6:1:1722:598 -TGAGTTTATTGCTGCCGTCATTGCTTATTATGTTCT ->HWI-EAS91_1_306UPAAXX:6:1:670:1188 -TTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGT ->HWI-EAS91_1_306UPAAXX:6:1:1682:1705 -TAGCCACATAGAAACCAACAGCCATATAACTGGTAG ->HWI-EAS91_1_306UPAAXX:6:1:1008:1616 -TCCTTTACTTGTCATGCGCTCTAATCTCTGTGCATC ->HWI-EAS91_1_306UPAAXX:6:1:490:1220 -TAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCT ->HWI-EAS91_1_306UPAAXX:6:1:891:1437 -TAATGGTGATATGTATGTTTACGTCCATAAGGCTGT ->HWI-EAS91_1_306UPAAXX:6:1:1310:321 -TCAATCCCCAATGCTTGGCTTCCATAAGCAGATGGT ->HWI-EAS91_1_306UPAAXX:6:1:827:1597 -TGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTC ->HWI-EAS91_1_306UPAAXX:6:1:1062:1158 -TAGAGTCAATAGCAAGGCCACGACGCAATGGAGAAA ->HWI-EAS91_1_306UPAAXX:6:1:1419:208 -TGGCGCATAATCTCGGAAACCTGCTGTTGCTTGGAA ->HWI-EAS91_1_306UPAAXX:6:1:691:1018 -AAATATCAACCACACCAGAAGCAGCATCAGTGACGA ->HWI-EAS91_1_306UPAAXX:6:1:374:113 -GATAAAGCTGTTGCCGATACTTGGAACAATTTCTGT ->HWI-EAS91_1_306UPAAXX:6:1:1720:784 -TGAGGATAAATTATGTCTAATATTCAAACTGGCGCC ->HWI-EAS91_1_306UPAAXX:6:1:1424:1394 -ATAAAAATGATTGGCGTATCCAACCTGCAGAGTTTT ->HWI-EAS91_1_306UPAAXX:6:1:1063:1760 -TAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCT ->HWI-EAS91_1_306UPAAXX:6:1:1235:729 -TTTTTATGTCCCTCATCGTCACGTTTATGGTGAACA ->HWI-EAS91_1_306UPAAXX:6:1:167:1507 -TAGTGTTATTAATATCAAGTTTTTGGAGCACATTGT ->HWI-EAS91_1_306UPAAXX:6:1:717:1569 -TCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGT ->HWI-EAS91_1_306UPAAXX:6:1:610:765 -TTCAGCGCCTTCCATGATGAGACAGGCCGTTTGAAT ->HWI-EAS91_1_306UPAAXX:6:1:663:380 -TAAACATTCTGTGCCGCGTTTCTTTGTTCCTTATCT ->HWI-EAS91_1_306UPAAXX:6:1:790:1358 -TTATCACCTTATTGAAGGCTTATCATTCATTTAGGT ->HWI-EAS91_1_306UPAAXX:6:1:965:1633 -TAGATGTGGTAGAAGTCGTCATTTGGCGAGAAAGCT ->HWI-EAS91_1_306UPAAXX:6:1:673:319 -TTCTTGCAAATCACCAGAAGGCGGTTCCTGAATGAT ->HWI-EAS91_1_306UPAAXX:6:1:684:371 -TAGCGGTAAAGTTAGACCAAACCATGAAACCAACAT ->HWI-EAS91_1_306UPAAXX:6:1:1147:1444 -ATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGC ->HWI-EAS91_1_306UPAAXX:6:1:983:678 -ATACCTGGTCTTTCGTATTCTGGCGTGAAGTCGCCG ->HWI-EAS91_1_306UPAAXX:6:1:1608:1119 -TCACGCGGCGGCAAGTTGCCATACAAAACAGGGTCG ->HWI-EAS91_1_306UPAAXX:6:1:1048:1193 -TAGTCAGGTTAAATTTAATGTGACCGTTTATCGCAT ->HWI-EAS91_1_306UPAAXX:6:1:1265:1429 -ATATTTTTCATGGTATTGATAAAGCTGTTGCCGATT ->HWI-EAS91_1_306UPAAXX:6:1:1607:1677 -TGTTGCTTGGAAAGATTGGTGTTTTCCATAATAGAC ->HWI-EAS91_1_306UPAAXX:6:1:1087:1421 -ACGAACGTCAGAAGCAGCCTTATGGCCGTCAACATC ->HWI-EAS91_1_306UPAAXX:6:1:324:490 -GCACCAAACATAAATCACCTCACTTAAGTGGCTGGG ->HWI-EAS91_1_306UPAAXX:6:1:1596:614 -TTACCGCTACTAAATGCCGCGGATTGGTTTCGCTGT ->HWI-EAS91_1_306UPAAXX:6:1:343:83 -GTTACGCAGTTTTGCCGCAAGCTGGCTGCTGTACGC ->HWI-EAS91_1_306UPAAXX:6:1:203:667 -GCATGAATGTGCTTAATAGAGGCCAAGGCGGTCTAG ->HWI-EAS91_1_306UPAAXX:6:1:34:480 -GGCAAGTTGCCATACAAAACAGGGTCGCCAGCAATT ->HWI-EAS91_1_306UPAAXX:6:1:606:1743 -TAGCGACAGCTTGGTTTTTAGTGAGTTGTTCCATTC ->HWI-EAS91_1_306UPAAXX:6:1:254:1391 -TATAATTACCCCAAAAAGAAAGGTATTAAGGATGAG ->HWI-EAS91_1_306UPAAXX:6:1:1568:1750 -TAACCAGTAGTGTTAACAGTCGGGAGAGGAGTGGCT ->HWI-EAS91_1_306UPAAXX:6:1:1538:869 -TACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCA ->HWI-EAS91_1_306UPAAXX:6:1:255:38 -GTCAGGATTGACACCCTCCCAATTGTATGTTTTCAT ->HWI-EAS91_1_306UPAAXX:6:1:1543:1555 -TAAAACGCCTCTAATCGGTCGTCAGCCAACGTGAGG ->HWI-EAS91_1_306UPAAXX:6:1:1365:733 -AGAATCAGCGGTATGGCTCTTCTCCTTTTTTCGCTT ->HWI-EAS91_1_306UPAAXX:6:1:1604:943 -TACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCT ->HWI-EAS91_1_306UPAAXX:6:1:1574:1632 -TCAGTATGCAAATTAGCATAAGCAGCTTGCAGACCC ->HWI-EAS91_1_306UPAAXX:6:1:565:1799 -TCTTGGTCAGTATGCAAATTAGCATAAGCAGCTTGC ->HWI-EAS91_1_306UPAAXX:6:1:1004:380 -TATTGACTCTACTGTAGACATTTTTACTTTTTATTT ->HWI-EAS91_1_306UPAAXX:6:1:1345:965 -ATTCAAAGGATAAACATCATAGGCAGTCGGGAGGGT ->HWI-EAS91_1_306UPAAXX:6:1:1704:756 -TGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGG ->HWI-EAS91_1_306UPAAXX:6:1:310:1346 -TATAACGTTGACGATGTAGCTTTAGTTTTCTTTAAA ->HWI-EAS91_1_306UPAAXX:6:1:900:1858 -TTTACCGCTTCGGCGTTATAACCTCACACTCAATCT ->HWI-EAS91_1_306UPAAXX:6:1:1250:1741 -TAAATCCAAAACGGCAGAAGCCTGAATGAGCTTAAT ->HWI-EAS91_1_306UPAAXX:6:1:1170:1317 -TCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTC ->HWI-EAS91_1_306UPAAXX:6:1:149:1896 -GCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATC ->HWI-EAS91_1_306UPAAXX:6:1:1504:494 -TGTCTACAGTAGAGTCAATAGCAAGGCCACGACGCC ->HWI-EAS91_1_306UPAAXX:6:1:395:256 -GTCCATATCTGACTTTTTGTTAACGTATTTATCCAC ->HWI-EAS91_1_306UPAAXX:6:1:1110:1109 -ACCGCTTCGGCGTTATAACCTCACACTCAATCTTTT ->HWI-EAS91_1_306UPAAXX:6:1:895:649 -TTCTGCACGTAATTTTTGACGCACGTTTTCTTCTGC ->HWI-EAS91_1_306UPAAXX:6:1:827:1378 -TGCAAGCTGCTTATGCTAATTTGCATACTGACCAAG ->HWI-EAS91_1_306UPAAXX:6:1:1051:1587 -TTTGACACTCTCACGTTGGCTGACGACCGATTAGAG ->HWI-EAS91_1_306UPAAXX:6:1:1656:1549 -AACCTGCTGTTGCTTGGAAAGATTGGTGTTTTCCAT ->HWI-EAS91_1_306UPAAXX:6:1:366:150 -GGTCAGTAGCAATCCAAACTTTGTTACTCGTCAGAA ->HWI-EAS91_1_306UPAAXX:6:1:955:1792 -ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA ->HWI-EAS91_1_306UPAAXX:6:1:1340:1403 -ATAAAATGCACCGCATGGAAATGAAGACGGCCATTA ->HWI-EAS91_1_306UPAAXX:6:1:1693:1017 -TGAGTTTGTATCTGTTACTGAGAAGTTAATGGATGT ->HWI-EAS91_1_306UPAAXX:6:1:1099:1572 -AATTTTTACCGCTTCGGCGTTATAACCTCACACTCA ->HWI-EAS91_1_306UPAAXX:6:1:218:1148 -TATGCAAATTAGCATAAGCAGCTTGCAGACCCATAT ->HWI-EAS91_1_306UPAAXX:6:1:403:614 -TGGTGCTGATGCTTCCTCTGCTGGTATGGTTTACGC ->HWI-EAS91_1_306UPAAXX:6:1:1651:646 -TCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGC ->HWI-EAS91_1_306UPAAXX:6:1:1566:499 -TGCGGTGCATTTTATGCGGACACTTCCTACAGGTAG ->HWI-EAS91_1_306UPAAXX:6:1:825:951 -ACAGGCCGTTTGAATGTTTACGGGGTGTACATAATA ->HWI-EAS91_1_306UPAAXX:6:1:1745:1865 -TTAACTTCTGCGTCATGGAAGCGATAAAACTCTGCG ->HWI-EAS91_1_306UPAAXX:6:1:973:1992 -TAGTAATTCCTGCTTTATCAAGATAATTTTTCGACT ->HWI-EAS91_1_306UPAAXX:6:1:171:1653 -TAATAATGTTTTCCGTAAATTCAGCGCCTTCCATGT ->HWI-EAS91_1_306UPAAXX:6:1:397:363 -TGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGT ->HWI-EAS91_1_306UPAAXX:6:1:1336:1155 -ATATGTATGTTGACGGCCATAAGGCTGCTTCTGACG ->HWI-EAS91_1_306UPAAXX:6:1:685:629 -AGTATGCAAATTAGCATAAGCAGCTTGCAGACCCAT ->HWI-EAS91_1_306UPAAXX:6:1:1409:510 -ACATAATAAGCAATGACGGCAGCAATAAACTCAACA ->HWI-EAS91_1_306UPAAXX:6:1:1631:998 -AACCATCAGCATGAGCCTGTCGCATTGCATTCATCC ->HWI-EAS91_1_306UPAAXX:6:1:260:1698 -TTATTATGTTCATCCCGTCAACATTCAAACTGCCTT ->HWI-EAS91_1_306UPAAXX:6:1:578:971 -TTAACGCTACTAAATTCCGCGGATTGGTTTCGTTGT ->HWI-EAS91_1_306UPAAXX:6:1:1613:642 -ATAGAAATTTCACGCGGCGGCAAGTTGCCATACAAA ->HWI-EAS91_1_306UPAAXX:6:1:237:650 -GACGGTATAATAACCACCATCATGGCGACCATTCAA ->HWI-EAS91_1_306UPAAXX:6:1:1100:1875 -TTATGGTTCGTTCTTATTACCCTTCTGAATGTCACG ->HWI-EAS91_1_306UPAAXX:6:1:352:32 -GTACCATAAACGCAAGCCTCAACGCAGCGACGAGCC ->HWI-EAS91_1_306UPAAXX:6:1:443:229 -GCAGTAGGCGGAAAACGAACAAGCGCAAGAGTAAAC ->HWI-EAS91_1_306UPAAXX:6:1:1131:731 -AGCAGTCGGCGTGTGAATCATTAGCCTTGCGACCCT ->HWI-EAS91_1_306UPAAXX:6:1:133:1089 -AAGGTTAGTGCTGAGGTTGACTTAGTTCATCATCAA ->HWI-EAS91_1_306UPAAXX:6:1:65:1307 -TGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTT ->HWI-EAS91_1_306UPAAXX:6:1:905:1493 -TCAGCTTTACCGTCTTTCCAGAAATTGTTCCAAGTT ->HWI-EAS91_1_306UPAAXX:6:1:733:540 -TGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAAT ->HWI-EAS91_1_306UPAAXX:6:1:161:1707 -TAATGTCGTCACTGATGCTGCTTCTGTTGTTGTTTT ->HWI-EAS91_1_306UPAAXX:6:1:380:1861 -TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC ->HWI-EAS91_1_306UPAAXX:6:1:1761:566 -TATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAT ->HWI-EAS91_1_306UPAAXX:6:1:1486:651 -TGGCGGTATTTCTTCTTCTCTTTCTTGTTGCGCCCT ->HWI-EAS91_1_306UPAAXX:6:1:508:1380 -TCCATCAACATCATAGCCAGATGCCCAGAGATTAGA ->HWI-EAS91_1_306UPAAXX:6:1:1763:855 -TGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTC ->HWI-EAS91_1_306UPAAXX:6:1:1553:553 -TAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAA ->HWI-EAS91_1_306UPAAXX:6:1:1424:507 -TCCACTGCAACAACTGAACGGACTGGAAACACTGGT ->HWI-EAS91_1_306UPAAXX:6:1:988:135 -TAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTC ->HWI-EAS91_1_306UPAAXX:6:1:810:1918 -TTTTCATCCCGAAGTTGCGGCTCATTCTGATTCTGT ->HWI-EAS91_1_306UPAAXX:6:1:588:559 -TCTGGTTGAACGGCGTCGCGTCGTAACCCAGCTTGG ->HWI-EAS91_1_306UPAAXX:6:1:1264:1214 -ATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC ->HWI-EAS91_1_306UPAAXX:6:1:1000:1475 -TCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTG ->HWI-EAS91_1_306UPAAXX:6:1:1389:160 -TTATTCGCCACCATGATTATGACCAGTGTTTCCAGT ->HWI-EAS91_1_306UPAAXX:6:1:422:1296 -TTCAACTACTCCGGTTATCGCTGGCGACTCCTTCGT ->HWI-EAS91_1_306UPAAXX:6:1:1273:856 -TAGCCATAGCACCAGAAACAAAACTAGGGGCGGCCT ->HWI-EAS91_1_306UPAAXX:6:1:450:969 -TGTTTTCCATAATAGACGCAACGCGAGCAGTAGACT ->HWI-EAS91_1_306UPAAXX:6:1:1202:828 -ATCGTCAACGTTATATTTTGATAGTTTGACGTTTAT ->HWI-EAS91_1_306UPAAXX:6:1:1721:1800 -GGGTTAGGGACATTAGAGCCTTGACTGACTGAGTTT ->HWI-EAS91_1_306UPAAXX:6:1:228:2019 -TTGAGTAAGCATTTGGCGCATAATCTCGGAAACCTG ->HWI-EAS91_1_306UPAAXX:6:1:1579:1214 -ACGTTTGGTCAGTTCCATCAACATCATAGCCAGATG ->HWI-EAS91_1_306UPAAXX:6:1:429:1055 -TTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTCTTT ->HWI-EAS91_1_306UPAAXX:6:1:1359:1689 -AAGAGCAGAAGCAATACCGCCAGCAATAGCACCAAA ->HWI-EAS91_1_306UPAAXX:6:1:1474:1056 -TCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGAC ->HWI-EAS91_1_306UPAAXX:6:1:105:1818 -TTGGGGATTGAGAAAGAGTAGAAATGCCACAAGCCT ->HWI-EAS91_1_306UPAAXX:6:1:208:1538 -TAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA ->HWI-EAS91_1_306UPAAXX:6:1:1361:1623 -AATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTA ->HWI-EAS91_1_306UPAAXX:6:1:595:1670 -TGAATCTCTTTAGTCGCAGTAGGCGGAAAACGAACA ->HWI-EAS91_1_306UPAAXX:6:1:6:1885 -TCTAATGTCGTCACTGATGCTGCTTCTGGTGTGTTT ->HWI-EAS91_1_306UPAAXX:6:1:706:1085 -TGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTG ->HWI-EAS91_1_306UPAAXX:6:1:1307:825 -AGCGGTAAAGTTAGACCAAACCATGAAACCAACATA ->HWI-EAS91_1_306UPAAXX:6:1:762:802 -TGGCATTAACACCATCCTTCATGAACTTAATCCACT ->HWI-EAS91_1_306UPAAXX:6:1:1657:506 -TTGCGACCCTCGGCAGCAAGAACCATACGACCAATT ->HWI-EAS91_1_306UPAAXX:6:1:184:811 -TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC ->HWI-EAS91_1_306UPAAXX:6:1:1469:1718 -TGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTTT ->HWI-EAS91_1_306UPAAXX:6:1:815:1640 -TGGCGGCGATTGCGTACCCGACGACCCAAATTAGGG ->HWI-EAS91_1_306UPAAXX:6:1:1580:1388 -AAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTG ->HWI-EAS91_1_306UPAAXX:6:1:1617:1554 -TACGGGGAAGGACGTCAATAGTCACACAGTCCTTGA ->HWI-EAS91_1_306UPAAXX:6:1:1544:431 -TGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTA ->HWI-EAS91_1_306UPAAXX:6:1:1604:1541 -TCAGTGACGACATTAGAAATATCCTTTGCAGTAGCG ->HWI-EAS91_1_306UPAAXX:6:1:1485:741 -ATCAAACGCTGAATAGTAAAGCCTCTACGCGATTTC ->HWI-EAS91_1_306UPAAXX:6:1:1226:393 -TGCCACAAGCCTCAATAGCAGGTTTAAGAGCCTCGA ->HWI-EAS91_1_306UPAAXX:6:1:1506:973 -ATTAGGGTCAACGCTACCTGTAGGAAGTGTCCGCAT ->HWI-EAS91_1_306UPAAXX:6:1:890:1838 -TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC ->HWI-EAS91_1_306UPAAXX:6:1:453:1527 -TAAGAGGGCGTTCAGCAGCCAGCTTGCGGCAAAACT ->HWI-EAS91_1_306UPAAXX:6:1:1056:570 -ACATTGTAGCATTGTGCCAATTCATCCATTAACTTC ->HWI-EAS91_1_306UPAAXX:6:1:1736:74 -TATCCGAAAGTGTTAACTTCTGCGTCATGGAAGCGT ->HWI-EAS91_1_306UPAAXX:6:1:169:1896 -GTATGCAAATTAGCATAAGCAGCTTGCAGACCCATA ->HWI-EAS91_1_306UPAAXX:6:1:259:949 -TGAGGATAAATTATGTCTAATATTCAAACTTGCTCC ->HWI-EAS91_1_306UPAAXX:6:1:1205:893 -ATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCT ->HWI-EAS91_1_306UPAAXX:6:1:732:1335 -TACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCT ->HWI-EAS91_1_306UPAAXX:6:1:667:664 -TCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAG ->HWI-EAS91_1_306UPAAXX:6:1:535:587 -TTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGT ->HWI-EAS91_1_306UPAAXX:6:1:412:446 -GTGTGGTTGATATTTTTCATGGTATTGATAAAGCTT ->HWI-EAS91_1_306UPAAXX:6:1:507:1599 -TTGCTGGCGGTTTTTCTTTTTTTTTTTTTTTTTTTT ->HWI-EAS91_1_306UPAAXX:6:1:575:1818 -TAAAATGCACCGCATGGAAATGAAGACGGCCATTAG ->HWI-EAS91_1_306UPAAXX:6:1:1568:1428 -ACCAGTTATATGGCTGGTTGTTTTTTTTTTTTTTTT ->HWI-EAS91_1_306UPAAXX:6:1:1417:982 -AACAAGAGAATCTCTACCATGAACAAAATGTGACTC ->HWI-EAS91_1_306UPAAXX:6:1:280:1340 -GGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTTT ->HWI-EAS91_1_306UPAAXX:6:1:966:144 -TACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGT ->HWI-EAS91_1_306UPAAXX:6:1:1391:1987 -TAATAATGTTTTCCGTAAATTCAGCGCCTTCCATGT ->HWI-EAS91_1_306UPAAXX:6:1:1280:278 -ATGGAAATGAAGACGGCCATTAGCTGTACCATACTC ->HWI-EAS91_1_306UPAAXX:6:1:631:858 -TGATATTGGTCGTATGGTTCTTGCTTCCGTGGGTCT ->HWI-EAS91_1_306UPAAXX:6:1:518:573 -TTAGGTGTCTGTAAAACAGGTGCCGAAGAAGCTGGT ->HWI-EAS91_1_306UPAAXX:6:1:54:981 -TTGACATTTTAAAAGAGCGTGGATTACTATCTGATT ->HWI-EAS91_1_306UPAAXX:6:1:218:1165 -TATTGACTCTACTGTAGACATTTTTACTTTTTATTT ->HWI-EAS91_1_306UPAAXX:6:1:1727:1530 -TCAACGCAGCGACGAGCACGAGAGCGGTCAGTAGCA ->HWI-EAS91_1_306UPAAXX:6:1:519:657 -TGAACAGCATCGGACTCAGATAGTAATCCACGCTCT ->HWI-EAS91_1_306UPAAXX:6:1:939:967 -ATACCGTCAAGGACTGTGTGACTATTGACGTCCTTC ->HWI-EAS91_1_306UPAAXX:6:1:299:1060 -TATAACTGGTAGCTTTAAGCGGCTCACCTTTAGCAT ->HWI-EAS91_1_306UPAAXX:6:1:438:665 -TAATTCGTAAACAAGCAGTAGTAATTCCTGCTTTAT ->HWI-EAS91_1_306UPAAXX:6:1:1303:1971 -AGCATTGTGCCAATTCATCCATTAACTTCTCAGTAA ->HWI-EAS91_1_306UPAAXX:6:1:214:1264 -TCAGCACCAACAGAAACAACCTGATTAGCGGCGTTG ->HWI-EAS91_1_306UPAAXX:6:1:1454:1423 -AACGGAAAACATCCTTCATAGAAATTTCACGCGGCG ->HWI-EAS91_1_306UPAAXX:6:1:1633:340 -TTCCATAATAGACGCAACGCGAGCAGTAGACTCCTT ->HWI-EAS91_1_306UPAAXX:6:1:671:1196 -ATACGAAAAGACAGAATCTCTTCCAAGAGCTTGATG diff -r 8d149264b777 -r efdb6fb5de34 test-data/b1.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/b1.fasta Fri Nov 27 10:53:50 2009 -0500 @@ -0,0 +1,1000 @@ +>HWI-EAS91_1_306UPAAXX:6:1:1503:1160 +GGTGGTCTATAGTGTTATTAATATCAAGTTGGGGGG +>HWI-EAS91_1_306UPAAXX:6:1:1564:1179 +GCGAGCAGTAGACTCCTTCTGTTGATAAGCAAGCAT +>HWI-EAS91_1_306UPAAXX:6:1:1704:1082 +GATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTC +>HWI-EAS91_1_306UPAAXX:6:1:1588:1797 +GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT +>HWI-EAS91_1_306UPAAXX:6:1:1304:1526 +GTAGTTGAAATGGTAATAAGACGACCAATCTGACCT +>HWI-EAS91_1_306UPAAXX:6:1:1490:1582 +GTCGTGTTCAACAGACCTATAAACATTCTGTGCCGC +>HWI-EAS91_1_306UPAAXX:6:1:1356:1339 +GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC +>HWI-EAS91_1_306UPAAXX:6:1:1311:853 +GGTTGGTTTATCGTTTTTGACACTCTCACGTTGTCT +>HWI-EAS91_1_306UPAAXX:6:1:1257:1552 +GTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTC +>HWI-EAS91_1_306UPAAXX:6:1:1486:1402 +GTTACTGAGAAGTTAATGGATGAATTGGCACAATGC +>HWI-EAS91_1_306UPAAXX:6:1:1028:1081 +GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT +>HWI-EAS91_1_306UPAAXX:6:1:1167:752 +GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC +>HWI-EAS91_1_306UPAAXX:6:1:1507:1113 +GTCAACGTTATATTTTGATAGTTTGACGGTTAATTC +>HWI-EAS91_1_306UPAAXX:6:1:1654:1311 +GGATGAAAATGCTCACAATGACAAATCTGTCCACGG +>HWI-EAS91_1_306UPAAXX:6:1:1386:1060 +GTTCTTGGTCAGTATGCAAATTAGCATAAGCAGCTT +>HWI-EAS91_1_306UPAAXX:6:1:1070:1356 +GGTTACAGTATGCCCATCGCAGTTCGCTACACGCAG +>HWI-EAS91_1_306UPAAXX:6:1:787:1032 +GCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCA +>HWI-EAS91_1_306UPAAXX:6:1:834:1017 +GCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGT +>HWI-EAS91_1_306UPAAXX:6:1:1703:1155 +GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT +>HWI-EAS91_1_306UPAAXX:6:1:1406:593 +GTTGAGTTCGATAATGGTGATATGTATGTTTACGGC +>HWI-EAS91_1_306UPAAXX:6:1:1411:886 +GTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCAT +>HWI-EAS91_1_306UPAAXX:6:1:923:972 +GCATGACAAGTAAAGGACGGTTGTCAGCGTCATAAG +>HWI-EAS91_1_306UPAAXX:6:1:1279:1004 +GCCATAGCACCAGAAACAAAACTAGGGGCGGCCTCT +>HWI-EAS91_1_306UPAAXX:6:1:1070:840 +GGTTGTCAGCGTCATAAGAGGTTTTACCTCCAAATG +>HWI-EAS91_1_306UPAAXX:6:1:1595:1040 +GTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGT +>HWI-EAS91_1_306UPAAXX:6:1:1002:559 +GAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAG +>HWI-EAS91_1_306UPAAXX:6:1:999:974 +GTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGC +>HWI-EAS91_1_306UPAAXX:6:1:896:982 +GTGGCTGGAGACAAATAATCTCTTTAATAACCTTAT +>HWI-EAS91_1_306UPAAXX:6:1:1366:741 +GTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGC +>HWI-EAS91_1_306UPAAXX:6:1:749:1469 +GTTTATGGTGAACAGTGGATTAAGTTCATGAAGGAT +>HWI-EAS91_1_306UPAAXX:6:1:1010:592 +GAGTTTATTGCTGCCGTCATTGCTTATTATGTTCAT +>HWI-EAS91_1_306UPAAXX:6:1:1393:650 +GTGACTCATATCTAAACCAGTCCTTGACGAACGTGC +>HWI-EAS91_1_306UPAAXX:6:1:1238:1731 +GAGAAATAAAAGTCTGAAACATGATTAAACTCCTAA +>HWI-EAS91_1_306UPAAXX:6:1:1629:908 +GATGCGGTTATCCATCTGCTTATGGAAGCCAAGCAT +>HWI-EAS91_1_306UPAAXX:6:1:1560:849 +GCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAG +>HWI-EAS91_1_306UPAAXX:6:1:1029:783 +GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT +>HWI-EAS91_1_306UPAAXX:6:1:1152:1324 +GACAATCAGAAAGAGATTGCCGAGATGCAAAATGAG +>HWI-EAS91_1_306UPAAXX:6:1:1614:2042 +GAAATGCCACAAGCCTCAATAGCAGGTTTAAGAGCC +>HWI-EAS91_1_306UPAAXX:6:1:1398:439 +GATGGTTGGTTTATCGTTTTTGACACTCTCACGTTG +>HWI-EAS91_1_306UPAAXX:6:1:955:616 +GACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGC +>HWI-EAS91_1_306UPAAXX:6:1:1672:753 +GAATGCCAGCAATCTCTTTTTGAGTCTCATTTTGCT +>HWI-EAS91_1_306UPAAXX:6:1:1195:1293 +GCAATGCGACAGGCTCATGCTGATGGTTGGTTTATC +>HWI-EAS91_1_306UPAAXX:6:1:1074:755 +GCAAGAGTAAACATAGTGCCATGCTCAGGAACAAAG +>HWI-EAS91_1_306UPAAXX:6:1:984:499 +GACTTAGTTCATCAGCAAACGCAGAATCAGCGGTAT +>HWI-EAS91_1_306UPAAXX:6:1:1452:1833 +GCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGT +>HWI-EAS91_1_306UPAAXX:6:1:863:710 +GAGTTCGATAATGGTGATATGTATGTTGACGGCCAT +>HWI-EAS91_1_306UPAAXX:6:1:885:649 +GCAGAAGTTAACACTTTCGGATATTTCTGATGAGTC +>HWI-EAS91_1_306UPAAXX:6:1:917:1214 +GACAGATGTATCCATCTGAATGCAATGAAGAAAACC +>HWI-EAS91_1_306UPAAXX:6:1:892:1254 +GCTCAGGAAATGCAGCAGCAAGATAATCACGAGTAT +>HWI-EAS91_1_306UPAAXX:6:1:1555:1005 +GCATTTGGCGCATAATCTCGGAAACCTGCTGTTGCT +>HWI-EAS91_1_306UPAAXX:6:1:1637:1413 +GATGCTGTTCAACCACTAATAGGTAAGAAATCATGT +>HWI-EAS91_1_306UPAAXX:6:1:1102:1567 +GGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAA +>HWI-EAS91_1_306UPAAXX:6:1:799:1337 +GTATATGCACAAAATGAGATGCTTGCTTATCAACAG +>HWI-EAS91_1_306UPAAXX:6:1:1353:1843 +GCAGACCCATAATGTCAATAGATGTGGTAGAAGTCG +>HWI-EAS91_1_306UPAAXX:6:1:1196:789 +GCGGCATACGCTCGGCGCCAGTTTGAATATTAGACA +>HWI-EAS91_1_306UPAAXX:6:1:1056:1676 +GTAAAATACTGACCAGCCGTTTGAGCTTGAGTAAGC +>HWI-EAS91_1_306UPAAXX:6:1:1349:1836 +GGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTC +>HWI-EAS91_1_306UPAAXX:6:1:1027:788 +GGTGTTAATGCCACTCCTCTCCCGACTGTTAACACT +>HWI-EAS91_1_306UPAAXX:6:1:990:1283 +GCTTAGGGATTTTATTGGTATCAGGGTTAATCGTGC +>HWI-EAS91_1_306UPAAXX:6:1:904:939 +GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT +>HWI-EAS91_1_306UPAAXX:6:1:1732:793 +GTCAACATACATATCACCATTATCGAACTCAACGCC +>HWI-EAS91_1_306UPAAXX:6:1:1355:2003 +GTTAGACCAAACCATGAAACCAACATAAACATTATT +>HWI-EAS91_1_306UPAAXX:6:1:1337:977 +GCACCAGAAACAAAACTAGGGGCGGCCTCATCAGGG +>HWI-EAS91_1_306UPAAXX:6:1:1605:1175 +GGAGGTAAAACCTCTTATGACGCTGACAACCGTCCT +>HWI-EAS91_1_306UPAAXX:6:1:1763:1192 +GACAGGCCGTTTGAATGTTGACGGGATGAACATAAT +>HWI-EAS91_1_306UPAAXX:6:1:722:483 +GTTATTATACCGTCAAGGACTGTGTGACTATTGACT +>HWI-EAS91_1_306UPAAXX:6:1:1760:1136 +GCAAAGCATTGGGATTATCATAAAACGCCTCTAATC +>HWI-EAS91_1_306UPAAXX:6:1:1088:798 +GGAAACCTGCTGTTGCTTGGAAAGATTGGTGTTTTC +>HWI-EAS91_1_306UPAAXX:6:1:633:1076 +GCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAG +>HWI-EAS91_1_306UPAAXX:6:1:673:754 +GTCATGGAAGCGATAAAACTCTGCAGGTTGGATATT +>HWI-EAS91_1_306UPAAXX:6:1:1759:2019 +GTAAAGGACGGTTGTCAGCGTCATAAGAGGTTTTAC +>HWI-EAS91_1_306UPAAXX:6:1:1064:1797 +GCGGTTATCCATCTGCTTATGGAAGCCAAGCATTGG +>HWI-EAS91_1_306UPAAXX:6:1:1112:1669 +GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACT +>HWI-EAS91_1_306UPAAXX:6:1:510:1447 +GCATTAAGCTCAGGAAATGCAGCAGCAAGATAATCA +>HWI-EAS91_1_306UPAAXX:6:1:877:1573 +GTGCTATTGCTGGCGGTATTTCTTCTTCTTTTTTTT +>HWI-EAS91_1_306UPAAXX:6:1:870:542 +GAATGTCACGCTGATTATTTTGACTTTGAGCGTATC +>HWI-EAS91_1_306UPAAXX:6:1:966:384 +GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA +>HWI-EAS91_1_306UPAAXX:6:1:1186:1903 +GCCAGCGATAACCGGAGTAGTTGAAATGGTAATAAG +>HWI-EAS91_1_306UPAAXX:6:1:1632:1742 +GCATCACCCATGCCTACAGTATTGTTATCGGTAGCC +>HWI-EAS91_1_306UPAAXX:6:1:1521:559 +GAGAGCGCCAACGGCGTCCATCTCGAAGGAGTCGCC +>HWI-EAS91_1_306UPAAXX:6:1:683:454 +GCTTATTATGTTCATCCCGTCAACATTCAAACGTCC +>HWI-EAS91_1_306UPAAXX:6:1:112:1280 +GTTGGCGCTCTCCGTCTTTCTCCATTTCGTCGTGTC +>HWI-EAS91_1_306UPAAXX:6:1:891:381 +GACCAGGGCGAGCGCCAGAACGTTTTTTACCTTTAG +>HWI-EAS91_1_306UPAAXX:6:1:1348:958 +GATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTT +>HWI-EAS91_1_306UPAAXX:6:1:1785:1915 +GCCCCGAAGGGGACNANAAATGGTTTTTAGAGAACG +>HWI-EAS91_1_306UPAAXX:6:1:1418:42 +GTATGCCCATCGCAGTTCGCTACACGCAGGACGCTT +>HWI-EAS91_1_306UPAAXX:6:1:1421:743 +GGTCAACGCTACCTGTAGGAAGTGTCCGCATAAAAT +>HWI-EAS91_1_306UPAAXX:6:1:1079:790 +GCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGT +>HWI-EAS91_1_306UPAAXX:6:1:663:740 +GGTATTAAGGATGAGTGTTCAAGATTGCTGGATGCC +>HWI-EAS91_1_306UPAAXX:6:1:1245:413 +GTTTGAATGTTGACGGGATGAACATAATAAGCAATG +>HWI-EAS91_1_306UPAAXX:6:1:1378:1035 +GCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGG +>HWI-EAS91_1_306UPAAXX:6:1:903:1746 +GTACGGGGAAGGACGTCAATAGTCACACAGTCCTTG +>HWI-EAS91_1_306UPAAXX:6:1:1713:1134 +GGCGTACGGGGAAGGACGTCAATAGTCACACAGTCC +>HWI-EAS91_1_306UPAAXX:6:1:1246:1887 +GCTCTAATCTCTGGGCATCTGGCTATGATGTTGATG +>HWI-EAS91_1_306UPAAXX:6:1:872:1731 +GGGCGGCCTCATCAGGGTTAGGAACATTAGAGCCTT +>HWI-EAS91_1_306UPAAXX:6:1:1714:1582 +GCTTTCCTGCTCCTGTTGAGTTTATTGCTTCCGTCT +>HWI-EAS91_1_306UPAAXX:6:1:1785:763 +GNCGAGAAATAAAANNNTGAAACATGATTAAANTCC +>HWI-EAS91_1_306UPAAXX:6:1:1684:542 +GAAAAGACAGAATCTCTTCCAAGAGCTTGATGCGGT +>HWI-EAS91_1_306UPAAXX:6:1:1581:1665 +GACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATT +>HWI-EAS91_1_306UPAAXX:6:1:901:1581 +GTGCTGATATTGCTTTTGATGCCGACCCTAAATTTT +>HWI-EAS91_1_306UPAAXX:6:1:1128:239 +GGTTATTATACCGTCAAGGACTGTGTGACTATTGAC +>HWI-EAS91_1_306UPAAXX:6:1:969:441 +GGTAAGAAATCATGAGTCAAGTTACTGAACAATCCG +>HWI-EAS91_1_306UPAAXX:6:1:630:1087 +GCCACCATGATTATGACCAGTGTTTCCAGTCCGTTC +>HWI-EAS91_1_306UPAAXX:6:1:606:1852 +GGAGACAAATAATCTCTTTAATAACCTGATTCAGCG +>HWI-EAS91_1_306UPAAXX:6:1:489:1315 +GAAAGCTCAGTCTCAGGAGGAAGCGGAGCAGTCCAC +>HWI-EAS91_1_306UPAAXX:6:1:465:1983 +GAGCCAATACCATCAGCTTTACCGTCTTTCCAGAAA +>HWI-EAS91_1_306UPAAXX:6:1:559:1028 +GAGTGCTTAATCCAACTTACCAAGCTGGGTTACGAC +>HWI-EAS91_1_306UPAAXX:6:1:1655:1413 +GTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCG +>HWI-EAS91_1_306UPAAXX:6:1:980:605 +GCCGTTTGAATGTTGACGGGATGAACATAATAAGCA +>HWI-EAS91_1_306UPAAXX:6:1:1629:1865 +GAAAAGCGGCATGGTCAATATAACCAGTAGTGTTAT +>HWI-EAS91_1_306UPAAXX:6:1:1180:1920 +GCACTCCGTGGACAGATTTGTCATTGTGAGCATTTT +>HWI-EAS91_1_306UPAAXX:6:1:1116:383 +GCGCAGGAAACACTGACGTTCTTACTGACGCAGAAG +>HWI-EAS91_1_306UPAAXX:6:1:906:2041 +GTCACGTTTATGGTGAACAGTGGATTAAGTTCATGA +>HWI-EAS91_1_306UPAAXX:6:1:1514:157 +GTCAATAGATGTGGTAGAAGTCGTCATTTGGCGTGG +>HWI-EAS91_1_306UPAAXX:6:1:1032:1857 +GCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGT +>HWI-EAS91_1_306UPAAXX:6:1:638:609 +GATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAT +>HWI-EAS91_1_306UPAAXX:6:1:74:750 +GATAATCACGAGTATCCTTTCCTTTATCATCTTCAT +>HWI-EAS91_1_306UPAAXX:6:1:486:822 +GTTGACGATGTAGCTTTAGGTGTCTTTAAAACAGGT +>HWI-EAS91_1_306UPAAXX:6:1:899:473 +GAACAGCATCGGACTCAGATAGTAATCCACGCTCTT +>HWI-EAS91_1_306UPAAXX:6:1:1613:197 +GTGACATTCAGAAGGGTAATAAGAACGAACCATAAA +>HWI-EAS91_1_306UPAAXX:6:1:326:1747 +GTTGAGGCTTTCGTTTATTGTACGCTTTGCTTTTTT +>HWI-EAS91_1_306UPAAXX:6:1:1487:526 +GCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT +>HWI-EAS91_1_306UPAAXX:6:1:629:665 +GAAATGCAGCAGCAAGATAATCACGAGTATCCTTTC +>HWI-EAS91_1_306UPAAXX:6:1:766:744 +GGCCGTCAACATACATATCACCATTATCGAACTCAA +>HWI-EAS91_1_306UPAAXX:6:1:391:1771 +GTGGTTGATATTTTTCATGGTATTGATAAATCTTTT +>HWI-EAS91_1_306UPAAXX:6:1:591:1102 +GCTTTGCGTGACTATTTTCGTGATATTGTTCGTTTG +>HWI-EAS91_1_306UPAAXX:6:1:917:664 +GCCATGATGGTGGTTATTATACCGTCAAGGACTGTG +>HWI-EAS91_1_306UPAAXX:6:1:217:737 +GTTCAGTTGTTGCATTGGAATATTCAGTTTAAATTT +>HWI-EAS91_1_306UPAAXX:6:1:1047:839 +GACCATTCAAAGGATAAACATCATAGGCAGTCGGGG +>HWI-EAS91_1_306UPAAXX:6:1:558:1040 +GCCACCAGCAAGAGCAGAAGCAATACCGCCAGCAAT +>HWI-EAS91_1_306UPAAXX:6:1:1207:524 +GCCAATACCATCAGCTTTACCGTCTTTCCAGAAATT +>HWI-EAS91_1_306UPAAXX:6:1:708:1634 +GCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAG +>HWI-EAS91_1_306UPAAXX:6:1:576:1851 +GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAG +>HWI-EAS91_1_306UPAAXX:6:1:906:460 +GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC +>HWI-EAS91_1_306UPAAXX:6:1:693:1260 +GCGAAAGGTCGCAAAGTAAGAGCTTCTCGAGCTGCG +>HWI-EAS91_1_306UPAAXX:6:1:1373:286 +GGACACTTCCTACAGGTAGCGTTGACCCTAATTTTG +>HWI-EAS91_1_306UPAAXX:6:1:762:41 +GATACTTGGAACAATTTCTGGAAAGACGGTAAAGCT +>HWI-EAS91_1_306UPAAXX:6:1:475:1091 +GTCACACAGTCCTTGACGGTATAATAACCACCATCT +>HWI-EAS91_1_306UPAAXX:6:1:791:627 +GCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACC +>HWI-EAS91_1_306UPAAXX:6:1:336:1791 +GAAGGAGTCGCCAGCGATAACCGGAGTAGTTGAAAT +>HWI-EAS91_1_306UPAAXX:6:1:1483:943 +GCACGTAATTTTTGACGCACGTTTTCTTCTGCGTCT +>HWI-EAS91_1_306UPAAXX:6:1:641:1071 +GATGGGCATACTGTAACCATAAGGCCACGTATTTTG +>HWI-EAS91_1_306UPAAXX:6:1:196:755 +GAACGCCCTCTTAAGGATATTCGCGATGAGTATAAT +>HWI-EAS91_1_306UPAAXX:6:1:463:1398 +GTCATAAGAGGTTTTACCTCCAAATGAAGAAATAAC +>HWI-EAS91_1_306UPAAXX:6:1:1559:460 +GCTCACAATGACAAATCTGTCCACGGAGTGCTTAAT +>HWI-EAS91_1_306UPAAXX:6:1:1625:1561 +GAGGAGTGGCATTAACACCATCCTTCATGAACTTAC +>HWI-EAS91_1_306UPAAXX:6:1:1729:1588 +GCTGATAAAGGAAAGGATACTCGTGATTATCTTGCT +>HWI-EAS91_1_306UPAAXX:6:1:945:393 +GGCCTCATCAGGGTTAGGAACATTAGAGCCTTGAAT +>HWI-EAS91_1_306UPAAXX:6:1:298:1391 +GTAAAGTTAGACCAAACCATGAAACCAACATAAACA +>HWI-EAS91_1_306UPAAXX:6:1:1270:1500 +GAATTACTACTGCTTGTTTACGAATTAAATATATGT +>HWI-EAS91_1_306UPAAXX:6:1:481:1546 +GCTGGCATTCAGTCGGCGACTTCACGCCAGAATACG +>HWI-EAS91_1_306UPAAXX:6:1:473:1729 +GTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAT +>HWI-EAS91_1_306UPAAXX:6:1:801:1831 +GCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAAT +>HWI-EAS91_1_306UPAAXX:6:1:536:639 +GCCGACCCTAAATTTTTTGCCTGTTTGGTTCTCTTT +>HWI-EAS91_1_306UPAAXX:6:1:259:938 +GTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTG +>HWI-EAS91_1_306UPAAXX:6:1:907:1513 +GGCATGGGTGATGCTGGTATTAAATCTGCCATTCAC +>HWI-EAS91_1_306UPAAXX:6:1:372:1409 +GATGAGTATAATTACCCCAAAAAGAAAGGTATTAAG +>HWI-EAS91_1_306UPAAXX:6:1:485:1626 +GATGGCAGCAACGGAAACCATAACGAGCATCATCTT +>HWI-EAS91_1_306UPAAXX:6:1:583:1679 +GCTCAAAGTCAAAATAATCAGCGTGACATTCAGAAG +>HWI-EAS91_1_306UPAAXX:6:1:690:1610 +GACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTT +>HWI-EAS91_1_306UPAAXX:6:1:257:918 +GCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTTCT +>HWI-EAS91_1_306UPAAXX:6:1:818:33 +GTGTTAATGCCACTCCTCTCCCGACTGTTAACTCTG +>HWI-EAS91_1_306UPAAXX:6:1:541:1242 +GGGATTATCATAAAACGCCTCTAATCGGTCGTCAGC +>HWI-EAS91_1_306UPAAXX:6:1:1014:279 +GTAAAAATGTCTACAGTAGAGTCAATAGCAAGGCCC +>HWI-EAS91_1_306UPAAXX:6:1:672:1790 +GGCCGTTTGAATGTTGACGGGATGAACATAATAAGC +>HWI-EAS91_1_306UPAAXX:6:1:708:464 +GGAGACAAATAATCTCTTTAATAACCTGATTCAGCG +>HWI-EAS91_1_306UPAAXX:6:1:633:1486 +GGGAAAGGTCATGCGGCATACGCTCGGCGCCAGTTT +>HWI-EAS91_1_306UPAAXX:6:1:328:696 +GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT +>HWI-EAS91_1_306UPAAXX:6:1:259:1389 +GCGTACTTATTCGCCACCATGATTATTACCAGTGTT +>HWI-EAS91_1_306UPAAXX:6:1:1315:41 +GCTTTCCGTGATGTCACAGCCTGCTTTGATGTGTCG +>HWI-EAS91_1_306UPAAXX:6:1:1647:549 +GCTTAATCCAACTTACCAAGCTGGGTTACGACGCGC +>HWI-EAS91_1_306UPAAXX:6:1:300:886 +GTTCTTGGTCAGTATGCAAATTAGCATAAGCAGCTT +>HWI-EAS91_1_306UPAAXX:6:1:317:1411 +GTACGCTGTACTTTGTGGGATACCCTCGCTTTCCTT +>HWI-EAS91_1_306UPAAXX:6:1:321:1819 +GGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGT +>HWI-EAS91_1_306UPAAXX:6:1:631:70 +GTGGATTACTATCTGAGTCCGATGCTGTTCAACCAC +>HWI-EAS91_1_306UPAAXX:6:1:624:1040 +GCTGGCGACTCCTTCGAGATGGACGCCGTTTGCGCT +>HWI-EAS91_1_306UPAAXX:6:1:662:1187 +GGGAGAGGAGTGGCATTAACACCATCCTTCATGACC +>HWI-EAS91_1_306UPAAXX:6:1:1440:1959 +GAATCAGCGGTATGGCTCCTCTCCTATTTTTGCTTC +>HWI-EAS91_1_306UPAAXX:6:1:458:1629 +GCTGGTGGCGCCATGTCTAAATTTTTTGGAGGCGGT +>HWI-EAS91_1_306UPAAXX:6:1:216:790 +GGGATGAAAATGCTCACAATGACAAATCTGTCCACG +>HWI-EAS91_1_306UPAAXX:6:1:1407:1174 +TTACCTATTAGTGGTTGAACAGCATCGGACTCAGAT +>HWI-EAS91_1_306UPAAXX:6:1:999:1790 +GTCCTGCGTGTAGCGAACTGCGATGGGCATACTGTC +>HWI-EAS91_1_306UPAAXX:6:1:141:1994 +GGCTTTTTTATGGTTCGTTCTTATTACCCTTCTTAT +>HWI-EAS91_1_306UPAAXX:6:1:225:465 +GTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGC +>HWI-EAS91_1_306UPAAXX:6:1:649:1760 +GACCCATAATGTCAATAGATGTGGTAGAAGTCGTCT +>HWI-EAS91_1_306UPAAXX:6:1:300:986 +GTTGAACACGACCAGAAAACTGGCCTAACGACGTTT +>HWI-EAS91_1_306UPAAXX:6:1:478:605 +GAGACTGAGCTTTCTCGCCAAATGACGACTTCTACC +>HWI-EAS91_1_306UPAAXX:6:1:622:395 +GGTAGCTTTAAGCGGCTCACCTTTAGCATCAACAGG +>HWI-EAS91_1_306UPAAXX:6:1:1701:574 +GTAAAGCCTCTACGCGATTTCATAGTGGAGGCCTCC +>HWI-EAS91_1_306UPAAXX:6:1:646:59 +GGAAGTGTCCGCATAAAATGCACCGCATGGAAATGT +>HWI-EAS91_1_306UPAAXX:6:1:284:2031 +GACAGAATCGTTAGTTGATGGCGAAAGGTCGCAAAG +>HWI-EAS91_1_306UPAAXX:6:1:22:1009 +GATGGATACATCTGTCAACGCCGCTAATCAGGTTGT +>HWI-EAS91_1_306UPAAXX:6:1:47:1826 +GCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCG +>HWI-EAS91_1_306UPAAXX:6:1:1025:1236 +TGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGT +>HWI-EAS91_1_306UPAAXX:6:1:773:591 +GAGCAGGAAAGCGAGGGTATCCCACAAAGTCCAGCG +>HWI-EAS91_1_306UPAAXX:6:1:1753:527 +GGTGGCATTCAAGGTGATGTGCTTGCTACCGATAAC +>HWI-EAS91_1_306UPAAXX:6:1:426:1717 +GTAGCGCCAATATGAGAAGAGCCATACCGCTGATTC +>HWI-EAS91_1_306UPAAXX:6:1:959:818 +TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC +>HWI-EAS91_1_306UPAAXX:6:1:459:1344 +GCCTATGATGTTTATCCTTTGAATGGTCGCCATGAT +>HWI-EAS91_1_306UPAAXX:6:1:973:1367 +TTCGTGATGAGTTTGTATCTGTTACTGATAAGTTAT +>HWI-EAS91_1_306UPAAXX:6:1:201:871 +GATTAGAGGCGTTTTATGATAATCCCAATGCTTTTC +>HWI-EAS91_1_306UPAAXX:6:1:713:1672 +GGCGTACGGGGAAGGACGTCAATAGTCACACAGTCC +>HWI-EAS91_1_306UPAAXX:6:1:444:1435 +TTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTGTG +>HWI-EAS91_1_306UPAAXX:6:1:288:1136 +GCCTTCCATGATGAGACAGGCCGTTTTAATTTTTTC +>HWI-EAS91_1_306UPAAXX:6:1:1653:225 +GCAAGGCCACGACGCAATGGAGAAAGACGGAGAGCG +>HWI-EAS91_1_306UPAAXX:6:1:537:1764 +GCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAA +>HWI-EAS91_1_306UPAAXX:6:1:196:1854 +GTATCGAGGCTCTTAAACCTGCTATTTAGGCTTTTT +>HWI-EAS91_1_306UPAAXX:6:1:312:1707 +GCGTCATAAGAGGTTTTACCTCCAAATGAAGAAATA +>HWI-EAS91_1_306UPAAXX:6:1:651:183 +GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT +>HWI-EAS91_1_306UPAAXX:6:1:295:694 +GTGATTACTTCATGCAGCGTTACCGTGATGTTATTT +>HWI-EAS91_1_306UPAAXX:6:1:330:1895 +GCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTG +>HWI-EAS91_1_306UPAAXX:6:1:590:331 +GAAATTTCTATGAATGATGTTTTCCGTTCTGGTGAT +>HWI-EAS91_1_306UPAAXX:6:1:481:1687 +GCAGATTGCGATAAACGGTCACATTAAATTTAACCT +>HWI-EAS91_1_306UPAAXX:6:1:1112:1279 +TGTGCATATACCTGGTCTTTCGTATTCTGTCGTGAT +>HWI-EAS91_1_306UPAAXX:6:1:1099:1216 +TTAGAGCGCATGACAAGTAAAGGACGGTTGTCAGCG +>HWI-EAS91_1_306UPAAXX:6:1:221:1238 +GTATCCTTTCCTTTATCATCGGCAGACTTTTCACCT +>HWI-EAS91_1_306UPAAXX:6:1:1015:364 +GCCAGCGATAACCGGAGTAGTTGAAATGGTAATAAG +>HWI-EAS91_1_306UPAAXX:6:1:735:1806 +TGTTATTAATATCAAGTTGGGGGAGCACATTGTAGC +>HWI-EAS91_1_306UPAAXX:6:1:320:411 +GCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAGTG +>HWI-EAS91_1_306UPAAXX:6:1:1273:1031 +TTAAGGATATTCGCGATGAGTATAATTACCCCAAAA +>HWI-EAS91_1_306UPAAXX:6:1:1456:1088 +AATAATCAGCGTGACATTCAGAAGGGTAATAAGAAC +>HWI-EAS91_1_306UPAAXX:6:1:1365:307 +GACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAG +>HWI-EAS91_1_306UPAAXX:6:1:478:252 +GATGCGGTTATCCATCTGCTTATTGAAGCCAAGCAT +>HWI-EAS91_1_306UPAAXX:6:1:915:1232 +TATTAATAACACTATAGACCACCGCCCCGAAGGGGC +>HWI-EAS91_1_306UPAAXX:6:1:680:1357 +TTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGC +>HWI-EAS91_1_306UPAAXX:6:1:238:1279 +GCCGAAGCCCCTGCAATTAAAATTGTTGACCACCTA +>HWI-EAS91_1_306UPAAXX:6:1:1583:35 +GCAAATTAGCATAAGCAGCTTGCAGACCCATAATGT +>HWI-EAS91_1_306UPAAXX:6:1:502:283 +GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT +>HWI-EAS91_1_306UPAAXX:6:1:418:1730 +GAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGT +>HWI-EAS91_1_306UPAAXX:6:1:596:647 +GCCTCAACGCAGCGACGAGCACGAGAGCGGTCAGTA +>HWI-EAS91_1_306UPAAXX:6:1:92:1591 +GTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCC +>HWI-EAS91_1_306UPAAXX:6:1:430:1938 +GCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGTCC +>HWI-EAS91_1_306UPAAXX:6:1:212:527 +GGTATTGATAAAGCTGTTGCCGATACTTGTAACAAT +>HWI-EAS91_1_306UPAAXX:6:1:594:942 +GACGACATTAGAAATATCCTTTGCAGTAGCGCCAAT +>HWI-EAS91_1_306UPAAXX:6:1:169:1774 +GCCTTCCATGATGAGACAGGCCGTTTTAATTTTTAC +>HWI-EAS91_1_306UPAAXX:6:1:1090:210 +GGAGAGCGCCAACGGCGTCCATCTCGAAGGAGTCGC +>HWI-EAS91_1_306UPAAXX:6:1:589:96 +GGCGGCCCCATCAGGGTTAGGAACATTAGAGCCTTG +>HWI-EAS91_1_306UPAAXX:6:1:1477:1231 +TAGGAACATTAGAGCCTTGAATGGCAGATTTAATAC +>HWI-EAS91_1_306UPAAXX:6:1:707:1076 +TCTGACGTTCGTGATGAGTTTGTATCTTTTTCTTTG +>HWI-EAS91_1_306UPAAXX:6:1:749:1715 +GAACATAATAAGCAATGACGGCAGCAATAAACTCAA +>HWI-EAS91_1_306UPAAXX:6:1:1738:1884 +GCTCACCTTTAGCATCAACAGGCCACAACCAACCAG +>HWI-EAS91_1_306UPAAXX:6:1:1160:1088 +TCACATTTTGTTCATGGTAGAGATTCTCTTGTTGAC +>HWI-EAS91_1_306UPAAXX:6:1:517:119 +GCAAGGCTAATGATTCACACGCCGACTGCTATCAGT +>HWI-EAS91_1_306UPAAXX:6:1:1472:716 +TGGTAATGGTGGTTTTCTTCATTTCATTCAGTTTTT +>HWI-EAS91_1_306UPAAXX:6:1:281:441 +GAGCAGTAGACTCCTTCTGTTGATAAGCAAGCATCT +>HWI-EAS91_1_306UPAAXX:6:1:1101:324 +AATACCATCAGCTTTACCGTCTTTCCAGAAATTGTT +>HWI-EAS91_1_306UPAAXX:6:1:1225:1494 +TTCTCAAATCCGGCGTCAACCATACCAGCAGAGGAA +>HWI-EAS91_1_306UPAAXX:6:1:1509:1025 +TTCTTGCTGCCGAGGGTCGCAAGGCTATTGTTTCAC +>HWI-EAS91_1_306UPAAXX:6:1:592:510 +GATACCAATAAAATCCCTAAGCATTTGTTTCTGGTT +>HWI-EAS91_1_306UPAAXX:6:1:324:1729 +GAACAAAGAAACGCGGCACAGAATGTTTATAGGTCT +>HWI-EAS91_1_306UPAAXX:6:1:128:1925 +GGAACAACTCACTAAAAACCAAGCTGTCGCTACTTC +>HWI-EAS91_1_306UPAAXX:6:1:786:893 +TACGGGGAAGGACGTCAATAGTCACACAGTCCTTGC +>HWI-EAS91_1_306UPAAXX:6:1:248:955 +GCTACAATGTGCTCCCCCAACTTGATATTAATAACA +>HWI-EAS91_1_306UPAAXX:6:1:388:1127 +GATATTGGTCGTATGGTTCTTGCTGCCTAGTGTCTC +>HWI-EAS91_1_306UPAAXX:6:1:721:1156 +TCTGGTTGGTTGTGGCCTTTTTATGCTAAATGTTAG +>HWI-EAS91_1_306UPAAXX:6:1:1564:1468 +TTACTTTTTATGTCCCTCATCGTCACGTTTATGTTG +>HWI-EAS91_1_306UPAAXX:6:1:750:77 +GGCTCATTCTGATTCTGAACAGCTTCTTGGGAAGTA +>HWI-EAS91_1_306UPAAXX:6:1:405:487 +GTTGGATTAAGCACTCCGTGGACAGATTTGTCATTT +>HWI-EAS91_1_306UPAAXX:6:1:836:1204 +TTGCTTCTGCTCTTGCTTGTGGCGCCATGTCTAAAT +>HWI-EAS91_1_306UPAAXX:6:1:224:1548 +GCTGCCGTCATTGCTTATTATGTTCATCCCTTCAAC +>HWI-EAS91_1_306UPAAXX:6:1:931:1015 +TTAAGGTACTGAATCTCTTTAGTCGCAGTAGGCGGT +>HWI-EAS91_1_306UPAAXX:6:1:329:579 +GTCCCTCATCGTCACGTTTATGGTGAACAGTGGATT +>HWI-EAS91_1_306UPAAXX:6:1:260:1145 +GCTTGCGTTTATGGTACGCTGGACTTTTTGTGATAC +>HWI-EAS91_1_306UPAAXX:6:1:1523:1253 +TTGGTAAAATACTGACCAGCCGTTTGAGCTTGAGTA +>HWI-EAS91_1_306UPAAXX:6:1:326:1271 +GACCACTCGCGATTCAATCATGACTTCGTGATAAAT +>HWI-EAS91_1_306UPAAXX:6:1:213:622 +GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA +>HWI-EAS91_1_306UPAAXX:6:1:274:712 +GCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTG +>HWI-EAS91_1_306UPAAXX:6:1:1549:627 +TATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATGT +>HWI-EAS91_1_306UPAAXX:6:1:1714:737 +TCTTTCGTATTCTGGCGTGAAGTCGCCGACTGAATG +>HWI-EAS91_1_306UPAAXX:6:1:760:1217 +TACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTT +>HWI-EAS91_1_306UPAAXX:6:1:174:768 +GTTGGCTGACGACCGATTAGAGGCGTTTTTTTATAT +>HWI-EAS91_1_306UPAAXX:6:1:172:1412 +GGTCGGCAGATTGCGATAAACGTTCACATTAAATTT +>HWI-EAS91_1_306UPAAXX:6:1:1393:869 +TTCATCCCGTCAACATTCAAACGGCCTGTCTCATCT +>HWI-EAS91_1_306UPAAXX:6:1:301:481 +GTTATAGATATTCAAATAACCCTGAAACAAATGCTT +>HWI-EAS91_1_306UPAAXX:6:1:648:1093 +TAACGCTGCATGAAGTAATCACGTTCTTGGTCAGTT +>HWI-EAS91_1_306UPAAXX:6:1:1233:591 +TTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCT +>HWI-EAS91_1_306UPAAXX:6:1:540:1415 +TTATTAAAGAGATTATTTTTCTCCAGCCACTTATGT +>HWI-EAS91_1_306UPAAXX:6:1:151:1792 +GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA +>HWI-EAS91_1_306UPAAXX:6:1:748:1378 +TGGATTACTATCTGAGTCCGATGCTGTTCAACCACT +>HWI-EAS91_1_306UPAAXX:6:1:1526:1479 +TGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCC +>HWI-EAS91_1_306UPAAXX:6:1:985:1093 +TAACCGTCTTCTCGTTCTCTAAAAACCATTTTTCTT +>HWI-EAS91_1_306UPAAXX:6:1:480:1378 +TCAACCTCAGCACTAACCTTGCGAGTCATTTCTTTG +>HWI-EAS91_1_306UPAAXX:6:1:903:753 +TGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAG +>HWI-EAS91_1_306UPAAXX:6:1:1697:1737 +GGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGT +>HWI-EAS91_1_306UPAAXX:6:1:803:1037 +TGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCTACT +>HWI-EAS91_1_306UPAAXX:6:1:1727:1244 +TTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCT +>HWI-EAS91_1_306UPAAXX:6:1:253:1162 +GCATTTAGTAGCGGTAAAGTTTGACCAAACCATTAT +>HWI-EAS91_1_306UPAAXX:6:1:216:856 +GTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCT +>HWI-EAS91_1_306UPAAXX:6:1:825:886 +TCCCACAAAGTCCAGCGTACCATAAACGCAAGCCTC +>HWI-EAS91_1_306UPAAXX:6:1:1699:962 +TGATTTCGATTTTCTGACGAGTAACAAAGTTTGGAT +>HWI-EAS91_1_306UPAAXX:6:1:1210:625 +TCAGATAGTAATCCACGCTCTTTTAAAATGTCAACA +>HWI-EAS91_1_306UPAAXX:6:1:538:616 +TAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGT +>HWI-EAS91_1_306UPAAXX:6:1:184:1849 +GCTCACCTTTAGCATCAACAGGCCACAACCAACCAG +>HWI-EAS91_1_306UPAAXX:6:1:1636:1103 +TATCTGACTTTTTGTTAACGTATTTAGCCACATAGA +>HWI-EAS91_1_306UPAAXX:6:1:605:223 +GGTTATTTGAATATCTATAACAACTATTTTAAATCG +>HWI-EAS91_1_306UPAAXX:6:1:256:1052 +GGTAAAGGACTTCTTGAAGGTACGTTGCAGTCTGGC +>HWI-EAS91_1_306UPAAXX:6:1:300:1515 +GCCATGATGGTGGTTATTATACCGTCAAGGACTTTT +>HWI-EAS91_1_306UPAAXX:6:1:1684:1320 +TGCTTGGCTTCCATAAGCAGATGGATAACCGCATCA +>HWI-EAS91_1_306UPAAXX:6:1:1186:895 +TCAGATGGATACATCTGTCAACGCCGCTAATCAGGT +>HWI-EAS91_1_306UPAAXX:6:1:1463:754 +TCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTT +>HWI-EAS91_1_306UPAAXX:6:1:808:1053 +TGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGG +>HWI-EAS91_1_306UPAAXX:6:1:960:1218 +TTTCTAATGTCGTCACTGATGCTGCTTCTGTTGTTT +>HWI-EAS91_1_306UPAAXX:6:1:521:1646 +GGAAAACGAACAAGCGCAAGAGTAAACATAGTGCCA +>HWI-EAS91_1_306UPAAXX:6:1:289:1885 +GCCAGCGATAACCGGAGTAGTTGAAATGGTAATAAG +>HWI-EAS91_1_306UPAAXX:6:1:471:170 +GGTCAGTTCCATCAACATCATAGCCAGATGCCCAGA +>HWI-EAS91_1_306UPAAXX:6:1:828:754 +TTTGCGTGACTATTTTCGTGATATTGTTCGTATGGT +>HWI-EAS91_1_306UPAAXX:6:1:924:1679 +TTTAATGTGACCGTTTATCGCAATCTGCCGACCACT +>HWI-EAS91_1_306UPAAXX:6:1:837:901 +TGCATTTTAGTAAGCTCTTTTTGATTCTCAAATCCG +>HWI-EAS91_1_306UPAAXX:6:1:543:16 +GCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTC +>HWI-EAS91_1_306UPAAXX:6:1:1482:578 +TCTTTAGCTCCTAGACCTTTAGCAGCAAGGTCCATA +>HWI-EAS91_1_306UPAAXX:6:1:1254:1668 +TTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCT +>HWI-EAS91_1_306UPAAXX:6:1:1402:898 +TCATGAGTCAAGTTACTGAACAATCCGTACGTTTCC +>HWI-EAS91_1_306UPAAXX:6:1:764:1534 +TTATACCGTCAAGGACTGTGTGACTATTGACGTCCT +>HWI-EAS91_1_306UPAAXX:6:1:681:1079 +TGGCGAATAAGTACGCGTTCTTGCAAATCACCAGAA +>HWI-EAS91_1_306UPAAXX:6:1:672:1350 +TTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTT +>HWI-EAS91_1_306UPAAXX:6:1:1266:493 +TGACCAGCCGTTTGAGCTTGAGTAAGCATTTGGCGC +>HWI-EAS91_1_306UPAAXX:6:1:118:238 +GACGGTATAATAACCACCATCATGGCGACCATTCAA +>HWI-EAS91_1_306UPAAXX:6:1:699:433 +TTATTGCCCGGCGTACGGGGAAGGACGTCAATAGTC +>HWI-EAS91_1_306UPAAXX:6:1:708:1387 +TGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTAC +>HWI-EAS91_1_306UPAAXX:6:1:498:1085 +TTATGATAATCCCAATGCTTTGCGTGACTATTTTCT +>HWI-EAS91_1_306UPAAXX:6:1:1101:1301 +TCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAAT +>HWI-EAS91_1_306UPAAXX:6:1:261:213 +GAATGGTCGCCATGATGGTGGTTATTATACCGTCAC +>HWI-EAS91_1_306UPAAXX:6:1:1287:1267 +TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGT +>HWI-EAS91_1_306UPAAXX:6:1:744:331 +TTAATGGATGAATTGGCACAATGCTACAATGTGCTC +>HWI-EAS91_1_306UPAAXX:6:1:614:814 +TGTCAGCGTCATAAGAGGTTTTACCTCCAAATGAAG +>HWI-EAS91_1_306UPAAXX:6:1:1362:1063 +TAAACGCAAGCCTCAACGCAGCGACGAGCACGAGAG +>HWI-EAS91_1_306UPAAXX:6:1:1238:1508 +TCAACTAACGATTCTGTCAAAAACTGACGCGTTGGT +>HWI-EAS91_1_306UPAAXX:6:1:904:1130 +TTATCGCAATCTGCCGACCACTCGCGATTCAATCAT +>HWI-EAS91_1_306UPAAXX:6:1:465:216 +GACCATGCCGCTTTTCTTGGCACGATTAACCCTTAT +>HWI-EAS91_1_306UPAAXX:6:1:844:628 +TAATGTCAATAGATGTGGTAGAAGTCGTCATTTGGC +>HWI-EAS91_1_306UPAAXX:6:1:684:1444 +TATCCCACAAAGTCCAGCGTACCATAAACGCAAGCC +>HWI-EAS91_1_306UPAAXX:6:1:515:1373 +TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCT +>HWI-EAS91_1_306UPAAXX:6:1:764:1667 +TTGAGTTCGATAATGGTGATATGTATGTTGACGTCC +>HWI-EAS91_1_306UPAAXX:6:1:1722:598 +TGAGTTTATTGCTGCCGTCATTGCTTATTATGTTCT +>HWI-EAS91_1_306UPAAXX:6:1:670:1188 +TTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGT +>HWI-EAS91_1_306UPAAXX:6:1:1682:1705 +TAGCCACATAGAAACCAACAGCCATATAACTGGTAG +>HWI-EAS91_1_306UPAAXX:6:1:1008:1616 +TCCTTTACTTGTCATGCGCTCTAATCTCTGTGCATC +>HWI-EAS91_1_306UPAAXX:6:1:490:1220 +TAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCT +>HWI-EAS91_1_306UPAAXX:6:1:891:1437 +TAATGGTGATATGTATGTTTACGTCCATAAGGCTGT +>HWI-EAS91_1_306UPAAXX:6:1:1310:321 +TCAATCCCCAATGCTTGGCTTCCATAAGCAGATGGT +>HWI-EAS91_1_306UPAAXX:6:1:827:1597 +TGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTC +>HWI-EAS91_1_306UPAAXX:6:1:1062:1158 +TAGAGTCAATAGCAAGGCCACGACGCAATGGAGAAA +>HWI-EAS91_1_306UPAAXX:6:1:1419:208 +TGGCGCATAATCTCGGAAACCTGCTGTTGCTTGGAA +>HWI-EAS91_1_306UPAAXX:6:1:691:1018 +AAATATCAACCACACCAGAAGCAGCATCAGTGACGA +>HWI-EAS91_1_306UPAAXX:6:1:374:113 +GATAAAGCTGTTGCCGATACTTGGAACAATTTCTGT +>HWI-EAS91_1_306UPAAXX:6:1:1720:784 +TGAGGATAAATTATGTCTAATATTCAAACTGGCGCC +>HWI-EAS91_1_306UPAAXX:6:1:1424:1394 +ATAAAAATGATTGGCGTATCCAACCTGCAGAGTTTT +>HWI-EAS91_1_306UPAAXX:6:1:1063:1760 +TAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCT +>HWI-EAS91_1_306UPAAXX:6:1:1235:729 +TTTTTATGTCCCTCATCGTCACGTTTATGGTGAACA +>HWI-EAS91_1_306UPAAXX:6:1:167:1507 +TAGTGTTATTAATATCAAGTTTTTGGAGCACATTGT +>HWI-EAS91_1_306UPAAXX:6:1:717:1569 +TCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGT +>HWI-EAS91_1_306UPAAXX:6:1:610:765 +TTCAGCGCCTTCCATGATGAGACAGGCCGTTTGAAT +>HWI-EAS91_1_306UPAAXX:6:1:663:380 +TAAACATTCTGTGCCGCGTTTCTTTGTTCCTTATCT +>HWI-EAS91_1_306UPAAXX:6:1:790:1358 +TTATCACCTTATTGAAGGCTTATCATTCATTTAGGT +>HWI-EAS91_1_306UPAAXX:6:1:965:1633 +TAGATGTGGTAGAAGTCGTCATTTGGCGAGAAAGCT +>HWI-EAS91_1_306UPAAXX:6:1:673:319 +TTCTTGCAAATCACCAGAAGGCGGTTCCTGAATGAT +>HWI-EAS91_1_306UPAAXX:6:1:684:371 +TAGCGGTAAAGTTAGACCAAACCATGAAACCAACAT +>HWI-EAS91_1_306UPAAXX:6:1:1147:1444 +ATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGC +>HWI-EAS91_1_306UPAAXX:6:1:983:678 +ATACCTGGTCTTTCGTATTCTGGCGTGAAGTCGCCG +>HWI-EAS91_1_306UPAAXX:6:1:1608:1119 +TCACGCGGCGGCAAGTTGCCATACAAAACAGGGTCG +>HWI-EAS91_1_306UPAAXX:6:1:1048:1193 +TAGTCAGGTTAAATTTAATGTGACCGTTTATCGCAT +>HWI-EAS91_1_306UPAAXX:6:1:1265:1429 +ATATTTTTCATGGTATTGATAAAGCTGTTGCCGATT +>HWI-EAS91_1_306UPAAXX:6:1:1607:1677 +TGTTGCTTGGAAAGATTGGTGTTTTCCATAATAGAC +>HWI-EAS91_1_306UPAAXX:6:1:1087:1421 +ACGAACGTCAGAAGCAGCCTTATGGCCGTCAACATC +>HWI-EAS91_1_306UPAAXX:6:1:324:490 +GCACCAAACATAAATCACCTCACTTAAGTGGCTGGG +>HWI-EAS91_1_306UPAAXX:6:1:1596:614 +TTACCGCTACTAAATGCCGCGGATTGGTTTCGCTGT +>HWI-EAS91_1_306UPAAXX:6:1:343:83 +GTTACGCAGTTTTGCCGCAAGCTGGCTGCTGTACGC +>HWI-EAS91_1_306UPAAXX:6:1:203:667 +GCATGAATGTGCTTAATAGAGGCCAAGGCGGTCTAG +>HWI-EAS91_1_306UPAAXX:6:1:34:480 +GGCAAGTTGCCATACAAAACAGGGTCGCCAGCAATT +>HWI-EAS91_1_306UPAAXX:6:1:606:1743 +TAGCGACAGCTTGGTTTTTAGTGAGTTGTTCCATTC +>HWI-EAS91_1_306UPAAXX:6:1:254:1391 +TATAATTACCCCAAAAAGAAAGGTATTAAGGATGAG +>HWI-EAS91_1_306UPAAXX:6:1:1568:1750 +TAACCAGTAGTGTTAACAGTCGGGAGAGGAGTGGCT +>HWI-EAS91_1_306UPAAXX:6:1:1538:869 +TACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCA +>HWI-EAS91_1_306UPAAXX:6:1:255:38 +GTCAGGATTGACACCCTCCCAATTGTATGTTTTCAT +>HWI-EAS91_1_306UPAAXX:6:1:1543:1555 +TAAAACGCCTCTAATCGGTCGTCAGCCAACGTGAGG +>HWI-EAS91_1_306UPAAXX:6:1:1365:733 +AGAATCAGCGGTATGGCTCTTCTCCTTTTTTCGCTT +>HWI-EAS91_1_306UPAAXX:6:1:1604:943 +TACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCT +>HWI-EAS91_1_306UPAAXX:6:1:1574:1632 +TCAGTATGCAAATTAGCATAAGCAGCTTGCAGACCC +>HWI-EAS91_1_306UPAAXX:6:1:565:1799 +TCTTGGTCAGTATGCAAATTAGCATAAGCAGCTTGC +>HWI-EAS91_1_306UPAAXX:6:1:1004:380 +TATTGACTCTACTGTAGACATTTTTACTTTTTATTT +>HWI-EAS91_1_306UPAAXX:6:1:1345:965 +ATTCAAAGGATAAACATCATAGGCAGTCGGGAGGGT +>HWI-EAS91_1_306UPAAXX:6:1:1704:756 +TGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGG +>HWI-EAS91_1_306UPAAXX:6:1:310:1346 +TATAACGTTGACGATGTAGCTTTAGTTTTCTTTAAA +>HWI-EAS91_1_306UPAAXX:6:1:900:1858 +TTTACCGCTTCGGCGTTATAACCTCACACTCAATCT +>HWI-EAS91_1_306UPAAXX:6:1:1250:1741 +TAAATCCAAAACGGCAGAAGCCTGAATGAGCTTAAT +>HWI-EAS91_1_306UPAAXX:6:1:1170:1317 +TCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTC +>HWI-EAS91_1_306UPAAXX:6:1:149:1896 +GCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATC +>HWI-EAS91_1_306UPAAXX:6:1:1504:494 +TGTCTACAGTAGAGTCAATAGCAAGGCCACGACGCC +>HWI-EAS91_1_306UPAAXX:6:1:395:256 +GTCCATATCTGACTTTTTGTTAACGTATTTATCCAC +>HWI-EAS91_1_306UPAAXX:6:1:1110:1109 +ACCGCTTCGGCGTTATAACCTCACACTCAATCTTTT +>HWI-EAS91_1_306UPAAXX:6:1:895:649 +TTCTGCACGTAATTTTTGACGCACGTTTTCTTCTGC +>HWI-EAS91_1_306UPAAXX:6:1:827:1378 +TGCAAGCTGCTTATGCTAATTTGCATACTGACCAAG +>HWI-EAS91_1_306UPAAXX:6:1:1051:1587 +TTTGACACTCTCACGTTGGCTGACGACCGATTAGAG +>HWI-EAS91_1_306UPAAXX:6:1:1656:1549 +AACCTGCTGTTGCTTGGAAAGATTGGTGTTTTCCAT +>HWI-EAS91_1_306UPAAXX:6:1:366:150 +GGTCAGTAGCAATCCAAACTTTGTTACTCGTCAGAA +>HWI-EAS91_1_306UPAAXX:6:1:955:1792 +ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA +>HWI-EAS91_1_306UPAAXX:6:1:1340:1403 +ATAAAATGCACCGCATGGAAATGAAGACGGCCATTA +>HWI-EAS91_1_306UPAAXX:6:1:1693:1017 +TGAGTTTGTATCTGTTACTGAGAAGTTAATGGATGT +>HWI-EAS91_1_306UPAAXX:6:1:1099:1572 +AATTTTTACCGCTTCGGCGTTATAACCTCACACTCA +>HWI-EAS91_1_306UPAAXX:6:1:218:1148 +TATGCAAATTAGCATAAGCAGCTTGCAGACCCATAT +>HWI-EAS91_1_306UPAAXX:6:1:403:614 +TGGTGCTGATGCTTCCTCTGCTGGTATGGTTTACGC +>HWI-EAS91_1_306UPAAXX:6:1:1651:646 +TCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGC +>HWI-EAS91_1_306UPAAXX:6:1:1566:499 +TGCGGTGCATTTTATGCGGACACTTCCTACAGGTAG +>HWI-EAS91_1_306UPAAXX:6:1:825:951 +ACAGGCCGTTTGAATGTTTACGGGGTGTACATAATA +>HWI-EAS91_1_306UPAAXX:6:1:1745:1865 +TTAACTTCTGCGTCATGGAAGCGATAAAACTCTGCG +>HWI-EAS91_1_306UPAAXX:6:1:973:1992 +TAGTAATTCCTGCTTTATCAAGATAATTTTTCGACT +>HWI-EAS91_1_306UPAAXX:6:1:171:1653 +TAATAATGTTTTCCGTAAATTCAGCGCCTTCCATGT +>HWI-EAS91_1_306UPAAXX:6:1:397:363 +TGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGT +>HWI-EAS91_1_306UPAAXX:6:1:1336:1155 +ATATGTATGTTGACGGCCATAAGGCTGCTTCTGACG +>HWI-EAS91_1_306UPAAXX:6:1:685:629 +AGTATGCAAATTAGCATAAGCAGCTTGCAGACCCAT +>HWI-EAS91_1_306UPAAXX:6:1:1409:510 +ACATAATAAGCAATGACGGCAGCAATAAACTCAACA +>HWI-EAS91_1_306UPAAXX:6:1:1631:998 +AACCATCAGCATGAGCCTGTCGCATTGCATTCATCC +>HWI-EAS91_1_306UPAAXX:6:1:260:1698 +TTATTATGTTCATCCCGTCAACATTCAAACTGCCTT +>HWI-EAS91_1_306UPAAXX:6:1:578:971 +TTAACGCTACTAAATTCCGCGGATTGGTTTCGTTGT +>HWI-EAS91_1_306UPAAXX:6:1:1613:642 +ATAGAAATTTCACGCGGCGGCAAGTTGCCATACAAA +>HWI-EAS91_1_306UPAAXX:6:1:237:650 +GACGGTATAATAACCACCATCATGGCGACCATTCAA +>HWI-EAS91_1_306UPAAXX:6:1:1100:1875 +TTATGGTTCGTTCTTATTACCCTTCTGAATGTCACG +>HWI-EAS91_1_306UPAAXX:6:1:352:32 +GTACCATAAACGCAAGCCTCAACGCAGCGACGAGCC +>HWI-EAS91_1_306UPAAXX:6:1:443:229 +GCAGTAGGCGGAAAACGAACAAGCGCAAGAGTAAAC +>HWI-EAS91_1_306UPAAXX:6:1:1131:731 +AGCAGTCGGCGTGTGAATCATTAGCCTTGCGACCCT +>HWI-EAS91_1_306UPAAXX:6:1:133:1089 +AAGGTTAGTGCTGAGGTTGACTTAGTTCATCATCAA +>HWI-EAS91_1_306UPAAXX:6:1:65:1307 +TGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTT +>HWI-EAS91_1_306UPAAXX:6:1:905:1493 +TCAGCTTTACCGTCTTTCCAGAAATTGTTCCAAGTT +>HWI-EAS91_1_306UPAAXX:6:1:733:540 +TGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAAT +>HWI-EAS91_1_306UPAAXX:6:1:161:1707 +TAATGTCGTCACTGATGCTGCTTCTGTTGTTGTTTT +>HWI-EAS91_1_306UPAAXX:6:1:380:1861 +TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC +>HWI-EAS91_1_306UPAAXX:6:1:1761:566 +TATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAT +>HWI-EAS91_1_306UPAAXX:6:1:1486:651 +TGGCGGTATTTCTTCTTCTCTTTCTTGTTGCGCCCT +>HWI-EAS91_1_306UPAAXX:6:1:508:1380 +TCCATCAACATCATAGCCAGATGCCCAGAGATTAGA +>HWI-EAS91_1_306UPAAXX:6:1:1763:855 +TGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTC +>HWI-EAS91_1_306UPAAXX:6:1:1553:553 +TAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAA +>HWI-EAS91_1_306UPAAXX:6:1:1424:507 +TCCACTGCAACAACTGAACGGACTGGAAACACTGGT +>HWI-EAS91_1_306UPAAXX:6:1:988:135 +TAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTC +>HWI-EAS91_1_306UPAAXX:6:1:810:1918 +TTTTCATCCCGAAGTTGCGGCTCATTCTGATTCTGT +>HWI-EAS91_1_306UPAAXX:6:1:588:559 +TCTGGTTGAACGGCGTCGCGTCGTAACCCAGCTTGG +>HWI-EAS91_1_306UPAAXX:6:1:1264:1214 +ATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC +>HWI-EAS91_1_306UPAAXX:6:1:1000:1475 +TCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTG +>HWI-EAS91_1_306UPAAXX:6:1:1389:160 +TTATTCGCCACCATGATTATGACCAGTGTTTCCAGT +>HWI-EAS91_1_306UPAAXX:6:1:422:1296 +TTCAACTACTCCGGTTATCGCTGGCGACTCCTTCGT +>HWI-EAS91_1_306UPAAXX:6:1:1273:856 +TAGCCATAGCACCAGAAACAAAACTAGGGGCGGCCT +>HWI-EAS91_1_306UPAAXX:6:1:450:969 +TGTTTTCCATAATAGACGCAACGCGAGCAGTAGACT +>HWI-EAS91_1_306UPAAXX:6:1:1202:828 +ATCGTCAACGTTATATTTTGATAGTTTGACGTTTAT +>HWI-EAS91_1_306UPAAXX:6:1:1721:1800 +GGGTTAGGGACATTAGAGCCTTGACTGACTGAGTTT +>HWI-EAS91_1_306UPAAXX:6:1:228:2019 +TTGAGTAAGCATTTGGCGCATAATCTCGGAAACCTG +>HWI-EAS91_1_306UPAAXX:6:1:1579:1214 +ACGTTTGGTCAGTTCCATCAACATCATAGCCAGATG +>HWI-EAS91_1_306UPAAXX:6:1:429:1055 +TTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTCTTT +>HWI-EAS91_1_306UPAAXX:6:1:1359:1689 +AAGAGCAGAAGCAATACCGCCAGCAATAGCACCAAA +>HWI-EAS91_1_306UPAAXX:6:1:1474:1056 +TCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGAC +>HWI-EAS91_1_306UPAAXX:6:1:105:1818 +TTGGGGATTGAGAAAGAGTAGAAATGCCACAAGCCT +>HWI-EAS91_1_306UPAAXX:6:1:208:1538 +TAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA +>HWI-EAS91_1_306UPAAXX:6:1:1361:1623 +AATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTA +>HWI-EAS91_1_306UPAAXX:6:1:595:1670 +TGAATCTCTTTAGTCGCAGTAGGCGGAAAACGAACA +>HWI-EAS91_1_306UPAAXX:6:1:6:1885 +TCTAATGTCGTCACTGATGCTGCTTCTGGTGTGTTT +>HWI-EAS91_1_306UPAAXX:6:1:706:1085 +TGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTG +>HWI-EAS91_1_306UPAAXX:6:1:1307:825 +AGCGGTAAAGTTAGACCAAACCATGAAACCAACATA +>HWI-EAS91_1_306UPAAXX:6:1:762:802 +TGGCATTAACACCATCCTTCATGAACTTAATCCACT +>HWI-EAS91_1_306UPAAXX:6:1:1657:506 +TTGCGACCCTCGGCAGCAAGAACCATACGACCAATT +>HWI-EAS91_1_306UPAAXX:6:1:184:811 +TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC +>HWI-EAS91_1_306UPAAXX:6:1:1469:1718 +TGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTTT +>HWI-EAS91_1_306UPAAXX:6:1:815:1640 +TGGCGGCGATTGCGTACCCGACGACCCAAATTAGGG +>HWI-EAS91_1_306UPAAXX:6:1:1580:1388 +AAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTG +>HWI-EAS91_1_306UPAAXX:6:1:1617:1554 +TACGGGGAAGGACGTCAATAGTCACACAGTCCTTGA +>HWI-EAS91_1_306UPAAXX:6:1:1544:431 +TGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTA +>HWI-EAS91_1_306UPAAXX:6:1:1604:1541 +TCAGTGACGACATTAGAAATATCCTTTGCAGTAGCG +>HWI-EAS91_1_306UPAAXX:6:1:1485:741 +ATCAAACGCTGAATAGTAAAGCCTCTACGCGATTTC +>HWI-EAS91_1_306UPAAXX:6:1:1226:393 +TGCCACAAGCCTCAATAGCAGGTTTAAGAGCCTCGA +>HWI-EAS91_1_306UPAAXX:6:1:1506:973 +ATTAGGGTCAACGCTACCTGTAGGAAGTGTCCGCAT +>HWI-EAS91_1_306UPAAXX:6:1:890:1838 +TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC +>HWI-EAS91_1_306UPAAXX:6:1:453:1527 +TAAGAGGGCGTTCAGCAGCCAGCTTGCGGCAAAACT +>HWI-EAS91_1_306UPAAXX:6:1:1056:570 +ACATTGTAGCATTGTGCCAATTCATCCATTAACTTC +>HWI-EAS91_1_306UPAAXX:6:1:1736:74 +TATCCGAAAGTGTTAACTTCTGCGTCATGGAAGCGT +>HWI-EAS91_1_306UPAAXX:6:1:169:1896 +GTATGCAAATTAGCATAAGCAGCTTGCAGACCCATA +>HWI-EAS91_1_306UPAAXX:6:1:259:949 +TGAGGATAAATTATGTCTAATATTCAAACTTGCTCC +>HWI-EAS91_1_306UPAAXX:6:1:1205:893 +ATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCT +>HWI-EAS91_1_306UPAAXX:6:1:732:1335 +TACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCT +>HWI-EAS91_1_306UPAAXX:6:1:667:664 +TCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAG +>HWI-EAS91_1_306UPAAXX:6:1:535:587 +TTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGT +>HWI-EAS91_1_306UPAAXX:6:1:412:446 +GTGTGGTTGATATTTTTCATGGTATTGATAAAGCTT +>HWI-EAS91_1_306UPAAXX:6:1:507:1599 +TTGCTGGCGGTTTTTCTTTTTTTTTTTTTTTTTTTT +>HWI-EAS91_1_306UPAAXX:6:1:575:1818 +TAAAATGCACCGCATGGAAATGAAGACGGCCATTAG +>HWI-EAS91_1_306UPAAXX:6:1:1568:1428 +ACCAGTTATATGGCTGGTTGTTTTTTTTTTTTTTTT +>HWI-EAS91_1_306UPAAXX:6:1:1417:982 +AACAAGAGAATCTCTACCATGAACAAAATGTGACTC +>HWI-EAS91_1_306UPAAXX:6:1:280:1340 +GGCCAAACCAGTGGCGATGGCCGCGCTGGAGGTTTT +>HWI-EAS91_1_306UPAAXX:6:1:966:144 +TACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGT +>HWI-EAS91_1_306UPAAXX:6:1:1391:1987 +TAATAATGTTTTCCGTAAATTCAGCGCCTTCCATGT +>HWI-EAS91_1_306UPAAXX:6:1:1280:278 +ATGGAAATGAAGACGGCCATTAGCTGTACCATACTC +>HWI-EAS91_1_306UPAAXX:6:1:631:858 +TGATATTGGTCGTATGGTTCTTGCTTCCGTGGGTCT +>HWI-EAS91_1_306UPAAXX:6:1:518:573 +TTAGGTGTCTGTAAAACAGGTGCCGAAGAAGCTGGT +>HWI-EAS91_1_306UPAAXX:6:1:54:981 +TTGACATTTTAAAAGAGCGTGGATTACTATCTGATT +>HWI-EAS91_1_306UPAAXX:6:1:218:1165 +TATTGACTCTACTGTAGACATTTTTACTTTTTATTT +>HWI-EAS91_1_306UPAAXX:6:1:1727:1530 +TCAACGCAGCGACGAGCACGAGAGCGGTCAGTAGCA +>HWI-EAS91_1_306UPAAXX:6:1:519:657 +TGAACAGCATCGGACTCAGATAGTAATCCACGCTCT +>HWI-EAS91_1_306UPAAXX:6:1:939:967 +ATACCGTCAAGGACTGTGTGACTATTGACGTCCTTC +>HWI-EAS91_1_306UPAAXX:6:1:299:1060 +TATAACTGGTAGCTTTAAGCGGCTCACCTTTAGCAT +>HWI-EAS91_1_306UPAAXX:6:1:438:665 +TAATTCGTAAACAAGCAGTAGTAATTCCTGCTTTAT +>HWI-EAS91_1_306UPAAXX:6:1:1303:1971 +AGCATTGTGCCAATTCATCCATTAACTTCTCAGTAA +>HWI-EAS91_1_306UPAAXX:6:1:214:1264 +TCAGCACCAACAGAAACAACCTGATTAGCGGCGTTG +>HWI-EAS91_1_306UPAAXX:6:1:1454:1423 +AACGGAAAACATCCTTCATAGAAATTTCACGCGGCG +>HWI-EAS91_1_306UPAAXX:6:1:1633:340 +TTCCATAATAGACGCAACGCGAGCAGTAGACTCCTT +>HWI-EAS91_1_306UPAAXX:6:1:671:1196 +ATACGAAAAGACAGAATCTCTTCCAAGAGCTTGATG diff -r 8d149264b777 -r efdb6fb5de34 test-data/lastz_wrapper_out1.sam --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/lastz_wrapper_out1.sam Fri Nov 27 10:53:50 2009 -0500 @@ -0,0 +1,494 @@ +@HD VN:1.0 SO:unsorted +@SQ SN:phiX LN:5386 +HWI-EAS91_1_306UPAAXX 16 phiX 4295 255 1H35M * 0 0 CCCCCAACTTGATATTAATAACACTATAGACCACC * +HWI-EAS91_1_306UPAAXX 16 phiX 3522 255 36M * 0 0 ATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGC * +HWI-EAS91_1_306UPAAXX 0 phiX 229 255 36M * 0 0 GATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTC * +HWI-EAS91_1_306UPAAXX 0 phiX 2071 255 36M * 0 0 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT * +HWI-EAS91_1_306UPAAXX 16 phiX 1067 255 1H35M * 0 0 GGTCAGATTGGTCGTCTTATTACCATTTCAACTAC * +HWI-EAS91_1_306UPAAXX 0 phiX 1788 255 36M * 0 0 GTCGTGTTCAACAGACCTATAAACATTCTGTGCCGC * +HWI-EAS91_1_306UPAAXX 0 phiX 1193 255 36M * 0 0 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC * +HWI-EAS91_1_306UPAAXX 0 phiX 4563 255 33M3H * 0 0 GGTTGGTTTATCGTTTTTGACACTCTCACGTTG * +HWI-EAS91_1_306UPAAXX 0 phiX 2668 255 36M * 0 0 GTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTC * +HWI-EAS91_1_306UPAAXX 0 phiX 4248 255 36M * 0 0 GTTACTGAGAAGTTAATGGATGAATTGGCACAATGC * +HWI-EAS91_1_306UPAAXX 0 phiX 2863 255 36M * 0 0 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT * +HWI-EAS91_1_306UPAAXX 0 phiX 2557 255 36M * 0 0 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC * +HWI-EAS91_1_306UPAAXX 0 phiX 2511 255 34M2H * 0 0 GTCAACGTTATATTTTGATAGTTTGACGGTTAAT * +HWI-EAS91_1_306UPAAXX 0 phiX 5152 255 36M * 0 0 GGATGAAAATGCTCACAATGACAAATCTGTCCACGG * +HWI-EAS91_1_306UPAAXX 16 phiX 1590 255 36M * 0 0 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC * +HWI-EAS91_1_306UPAAXX 0 phiX 4884 255 36M * 0 0 GGTTACAGTATGCCCATCGCAGTTCGCTACACGCAG * +HWI-EAS91_1_306UPAAXX 0 phiX 5052 255 36M * 0 0 GCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCA * +HWI-EAS91_1_306UPAAXX 0 phiX 4909 255 36M * 0 0 GCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGT * +HWI-EAS91_1_306UPAAXX 0 phiX 2863 255 36M * 0 0 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT * +HWI-EAS91_1_306UPAAXX 0 phiX 4173 255 30M6H * 0 0 GTTGAGTTCGATAATGGTGATATGTATGTT * +HWI-EAS91_1_306UPAAXX 0 phiX 1701 255 36M * 0 0 GTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCAT * +HWI-EAS91_1_306UPAAXX 16 phiX 1683 255 36M * 0 0 CTTATGACGCTGACAACCGTCCTTTACTTGTCATGC * +HWI-EAS91_1_306UPAAXX 16 phiX 3126 255 1H35M * 0 0 GAGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGC * +HWI-EAS91_1_306UPAAXX 16 phiX 1665 255 36M * 0 0 CATTTGGAGGTAAAACCTCTTATGACGCTGACAACC * +HWI-EAS91_1_306UPAAXX 0 phiX 3213 255 36M * 0 0 GTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGT * +HWI-EAS91_1_306UPAAXX 0 phiX 3420 255 36M * 0 0 GAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAG * +HWI-EAS91_1_306UPAAXX 0 phiX 513 255 36M * 0 0 GTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGC * +HWI-EAS91_1_306UPAAXX 16 phiX 2882 255 3H33M * 0 0 AGGTTATTAAAGAGATTATTTGTCTCCAGCCAC * +HWI-EAS91_1_306UPAAXX 0 phiX 4471 255 36M * 0 0 GTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGC * +HWI-EAS91_1_306UPAAXX 0 phiX 1229 255 36M * 0 0 GTTTATGGTGAACAGTGGATTAAGTTCATGAAGGAT * +HWI-EAS91_1_306UPAAXX 0 phiX 615 255 36M * 0 0 GAGTTTATTGCTGCCGTCATTGCTTATTATGTTCAT * +HWI-EAS91_1_306UPAAXX 16 phiX 257 255 36M * 0 0 GCACGTTCGTCAAGGACTGGTTTAGATATGAGTCAC * +HWI-EAS91_1_306UPAAXX 16 phiX 2381 255 36M * 0 0 TTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTC * +HWI-EAS91_1_306UPAAXX 16 phiX 4098 255 36M * 0 0 ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC * +HWI-EAS91_1_306UPAAXX 0 phiX 5099 255 36M * 0 0 GCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAG * +HWI-EAS91_1_306UPAAXX 0 phiX 4254 255 36M * 0 0 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT * +HWI-EAS91_1_306UPAAXX 0 phiX 3408 255 36M * 0 0 GACAATCAGAAAGAGATTGCCGAGATGCAAAATGAG * +HWI-EAS91_1_306UPAAXX 16 phiX 4043 255 36M * 0 0 GGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTC * +HWI-EAS91_1_306UPAAXX 0 phiX 4560 255 36M * 0 0 GATGGTTGGTTTATCGTTTTTGACACTCTCACGTTG * +HWI-EAS91_1_306UPAAXX 0 phiX 1888 255 36M * 0 0 GACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGC * +HWI-EAS91_1_306UPAAXX 16 phiX 3434 255 1H35M * 0 0 GCAAAATGAGACTCAAAAAGAGATTGCTGGCATTC * +HWI-EAS91_1_306UPAAXX 0 phiX 4539 255 36M * 0 0 GCAATGCGACAGGCTCATGCTGATGGTTGGTTTATC * +HWI-EAS91_1_306UPAAXX 16 phiX 1828 255 36M * 0 0 CTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGC * +HWI-EAS91_1_306UPAAXX 0 phiX 3708 255 36M * 0 0 GACTTAGTTCATCAGCAAACGCAGAATCAGCGGTAT * +HWI-EAS91_1_306UPAAXX 0 phiX 3326 255 36M * 0 0 GCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGT * +HWI-EAS91_1_306UPAAXX 0 phiX 4176 255 36M * 0 0 GAGTTCGATAATGGTGATATGTATGTTGACGGCCAT * +HWI-EAS91_1_306UPAAXX 0 phiX 22 255 36M * 0 0 GCAGAAGTTAACACTTTCGGATATTTCTGATGAGTC * +HWI-EAS91_1_306UPAAXX 16 phiX 2557 255 36M * 0 0 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC * +HWI-EAS91_1_306UPAAXX 16 phiX 3277 255 36M * 0 0 ATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGC * +HWI-EAS91_1_306UPAAXX 16 phiX 3592 255 36M * 0 0 AGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGC * +HWI-EAS91_1_306UPAAXX 0 phiX 358 255 35M1H * 0 0 GATGCTGTTCAACCACTAATAGGTAAGAAATCATG * +HWI-EAS91_1_306UPAAXX 0 phiX 1775 255 36M * 0 0 GGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAA * +HWI-EAS91_1_306UPAAXX 0 phiX 3504 255 36M * 0 0 GTATATGCACAAAATGAGATGCTTGCTTATCAACAG * +HWI-EAS91_1_306UPAAXX 16 phiX 1554 255 36M * 0 0 CGACTTCTACCACATCTATTGACATTATGGGTCTGC * +HWI-EAS91_1_306UPAAXX 16 phiX 1002 255 36M * 0 0 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC * +HWI-EAS91_1_306UPAAXX 16 phiX 3626 255 36M * 0 0 GCTTACTCAAGCTCAAACGGCTGGTCAGTATTTTAC * +HWI-EAS91_1_306UPAAXX 0 phiX 3572 255 36M * 0 0 GGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTC * +HWI-EAS91_1_306UPAAXX 0 phiX 1265 255 36M * 0 0 GGTGTTAATGCCACTCCTCTCCCGACTGTTAACACT * +HWI-EAS91_1_306UPAAXX 16 phiX 1332 255 36M * 0 0 GCACGATTAACCCTGATACCAATAAAATCCCTAAGC * +HWI-EAS91_1_306UPAAXX 0 phiX 4254 255 36M * 0 0 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT * +HWI-EAS91_1_306UPAAXX 16 phiX 4170 255 36M * 0 0 GGCGTTGAGTTCGATAATGGTGATATGTATGTTGAC * +HWI-EAS91_1_306UPAAXX 16 phiX 2812 255 6H30M * 0 0 GTTTATGTTGGTTTCATGGTTTGGTCTAAC * +HWI-EAS91_1_306UPAAXX 16 phiX 3119 255 36M * 0 0 CCCTGATGAGGCCGCCCCTAGTTTTGTTTCTGGTGC * +HWI-EAS91_1_306UPAAXX 0 phiX 1670 255 36M * 0 0 GGAGGTAAAACCTCTTATGACGCTGACAACCGTCCT * +HWI-EAS91_1_306UPAAXX 16 phiX 640 255 36M * 0 0 ATTATGTTCATCCCGTCAACATTCAAACGGCCTGTC * +HWI-EAS91_1_306UPAAXX 0 phiX 2750 255 35M1H * 0 0 GTTATTATACCGTCAAGGACTGTGTGACTATTGAC * +HWI-EAS91_1_306UPAAXX 16 phiX 4606 255 36M * 0 0 GATTAGAGGCGTTTTATGATAATCCCAATGCTTTGC * +HWI-EAS91_1_306UPAAXX 16 phiX 3573 255 36M * 0 0 GAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCC * +HWI-EAS91_1_306UPAAXX 0 phiX 5105 255 36M * 0 0 GCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAG * +HWI-EAS91_1_306UPAAXX 16 phiX 1 255 15H21M * 0 0 GAGTTTTATCGCTTCCATGAC * +HWI-EAS91_1_306UPAAXX 16 phiX 1674 255 36M * 0 0 GTAAAACCTCTTATGACGCTGACAACCGTCCTTTAC * +HWI-EAS91_1_306UPAAXX 16 phiX 4095 255 36M * 0 0 CCAATGCTTGGCTTCCATAAGCAGATGGATAACCGC * +HWI-EAS91_1_306UPAAXX 0 phiX 4551 255 36M * 0 0 GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACT * +HWI-EAS91_1_306UPAAXX 16 phiX 3284 255 36M * 0 0 TGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGC * +HWI-EAS91_1_306UPAAXX 0 phiX 2938 255 20M16H * 0 0 GTGCTATTGCTGGCGGTATT * +HWI-EAS91_1_306UPAAXX 0 phiX 4005 255 36M * 0 0 GAATGTCACGCTGATTATTTTGACTTTGAGCGTATC * +HWI-EAS91_1_306UPAAXX 0 phiX 2479 255 36M * 0 0 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA * +HWI-EAS91_1_306UPAAXX 16 phiX 1082 255 36M * 0 0 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC * +HWI-EAS91_1_306UPAAXX 16 phiX 3045 255 1H35M * 0 0 GCTACCGATAACAATACTGTAGGCATGGGTGATGC * +HWI-EAS91_1_306UPAAXX 16 phiX 1115 255 36M * 0 0 GGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTC * +HWI-EAS91_1_306UPAAXX 0 phiX 636 255 33M3H * 0 0 GCTTATTATGTTCATCCCGTCAACATTCAAACG * +HWI-EAS91_1_306UPAAXX 0 phiX 1139 255 34M2H * 0 0 GTTGGCGCTCTCCGTCTTTCTCCATTTCGTCGTG * +HWI-EAS91_1_306UPAAXX 16 phiX 852 255 36M * 0 0 CTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTC * +HWI-EAS91_1_306UPAAXX 0 phiX 5272 255 36M * 0 0 GATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTT * +HWI-EAS91_1_306UPAAXX 0 phiX 4330 255 36M * 0 0 GCCCCGAAGGGGACNANAAATGGTTTTTAGAGAACG * +HWI-EAS91_1_306UPAAXX 0 phiX 4891 255 36M * 0 0 GTATGCCCATCGCAGTTCGCTACACGCAGGACGCTT * +HWI-EAS91_1_306UPAAXX 16 phiX 4785 255 2H34M * 0 0 TTTATGCGGACACTTCCTACAGGTAGCGTTGACC * +HWI-EAS91_1_306UPAAXX 0 phiX 3619 255 36M * 0 0 GCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGT * +HWI-EAS91_1_306UPAAXX 0 phiX 4455 255 32M4H * 0 0 GGTATTAAGGATGAGTGTTCAAGATTGCTGGA * +HWI-EAS91_1_306UPAAXX 16 phiX 632 255 36M * 0 0 CATTGCTTATTATGTTCATCCCGTCAACATTCAAAC * +HWI-EAS91_1_306UPAAXX 0 phiX 2964 255 35M1H * 0 0 GCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGG * +HWI-EAS91_1_306UPAAXX 16 phiX 2763 255 36M * 0 0 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTAC * +HWI-EAS91_1_306UPAAXX 16 phiX 2766 255 36M * 0 0 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC * +HWI-EAS91_1_306UPAAXX 0 phiX 1719 255 36M * 0 0 GCTCTAATCTCTGGGCATCTGGCTATGATGTTGATG * +HWI-EAS91_1_306UPAAXX 16 phiX 3100 255 36M * 0 0 AAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCC * +HWI-EAS91_1_306UPAAXX 0 phiX 598 255 29M7H * 0 0 GCTTTCCTGCTCCTGTTGAGTTTATTGCT * +HWI-EAS91_1_306UPAAXX 16 phiX 4126 255 36M * 0 0 ACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTC * +HWI-EAS91_1_306UPAAXX 0 phiX 4026 255 36M * 0 0 GACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATT * +HWI-EAS91_1_306UPAAXX 0 phiX 2621 255 36M * 0 0 GTGCTGATATTGCTTTTGATGCCGACCCTAAATTTT * +HWI-EAS91_1_306UPAAXX 0 phiX 2749 255 36M * 0 0 GGTTATTATACCGTCAAGGACTGTGTGACTATTGAC * +HWI-EAS91_1_306UPAAXX 0 phiX 379 255 36M * 0 0 GGTAAGAAATCATGAGTCAAGTTACTGAACAATCCG * +HWI-EAS91_1_306UPAAXX 0 phiX 2160 255 36M * 0 0 GCCACCATGATTATGACCAGTGTTTCCAGTCCGTTC * +HWI-EAS91_1_306UPAAXX 16 phiX 2873 255 36M * 0 0 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC * +HWI-EAS91_1_306UPAAXX 16 phiX 1508 255 1H35M * 0 0 TGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTC * +HWI-EAS91_1_306UPAAXX 16 phiX 3863 255 36M * 0 0 TTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTC * +HWI-EAS91_1_306UPAAXX 0 phiX 5187 255 36M * 0 0 GAGTGCTTAATCCAACTTACCAAGCTGGGTTACGAC * +HWI-EAS91_1_306UPAAXX 0 phiX 4196 255 36M * 0 0 GTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCG * +HWI-EAS91_1_306UPAAXX 16 phiX 635 255 36M * 0 0 TGCTTATTATGTTCATCCCGTCAACATTCAAACGGC * +HWI-EAS91_1_306UPAAXX 16 phiX 1294 255 1H35M * 0 0 TAACACTACTGGTTATATTGACCATGCCGCTTTTC * +HWI-EAS91_1_306UPAAXX 16 phiX 5157 255 36M * 0 0 AAAATGCTCACAATGACAAATCTGTCCACGGAGTGC * +HWI-EAS91_1_306UPAAXX 0 phiX 772 255 36M * 0 0 GCGCAGGAAACACTGACGTTCTTACTGACGCAGAAG * +HWI-EAS91_1_306UPAAXX 0 phiX 1224 255 36M * 0 0 GTCACGTTTATGGTGAACAGTGGATTAAGTTCATGA * +HWI-EAS91_1_306UPAAXX 16 phiX 1544 255 3H33M * 0 0 CGCCAAATGACGACTTCTACCACATCTATTGAC * +HWI-EAS91_1_306UPAAXX 0 phiX 4840 255 36M * 0 0 GCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGT * +HWI-EAS91_1_306UPAAXX 0 phiX 204 255 35M1H * 0 0 GATTCTGTCAAAAACTGACGCGTTGGATGAGGAGA * +HWI-EAS91_1_306UPAAXX 16 phiX 3263 255 7H29M * 0 0 TGATAAAGGAAAGGATACTCGTGATTATC * +HWI-EAS91_1_306UPAAXX 16 phiX 2481 255 36M * 0 0 ACCTGTTTTAAAGACACCTAAAGCTACATCGTCAAC * +HWI-EAS91_1_306UPAAXX 16 phiX 332 255 36M * 0 0 AAGAGCGTGGATTACTATCTGAGTCCGATGCTGTTC * +HWI-EAS91_1_306UPAAXX 16 phiX 3978 255 36M * 0 0 TTTATGGTTCGTTCTTATTACCCTTCTGAATGTCAC * +HWI-EAS91_1_306UPAAXX 0 phiX 4865 255 36M * 0 0 GCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT * +HWI-EAS91_1_306UPAAXX 16 phiX 3271 255 36M * 0 0 GAAAGGATACTCGTGATTATCTTGCTGCTGCATTTC * +HWI-EAS91_1_306UPAAXX 16 phiX 4174 255 36M * 0 0 TTGAGTTCGATAATGGTGATATGTATGTTGACGGCC * +HWI-EAS91_1_306UPAAXX 0 phiX 3810 255 30M6H * 0 0 GTGGTTGATATTTTTCATGGTATTGATAAA * +HWI-EAS91_1_306UPAAXX 0 phiX 4635 255 28M8H * 0 0 GCTTTGCGTGACTATTTTCGTGATATTG * +HWI-EAS91_1_306UPAAXX 0 phiX 2738 255 36M * 0 0 GCCATGATGGTGGTTATTATACCGTCAAGGACTGTG * +HWI-EAS91_1_306UPAAXX 0 phiX 2192 255 22M14H * 0 0 GTTCAGTTGTTGCATTGGAATA * +HWI-EAS91_1_306UPAAXX 16 phiX 2703 255 1H35M * 0 0 CCCGACTGCCTATGATGTTTATCCTTTGAATGGTC * +HWI-EAS91_1_306UPAAXX 16 phiX 2943 255 36M * 0 0 ATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGC * +HWI-EAS91_1_306UPAAXX 16 phiX 3861 255 36M * 0 0 AATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGC * +HWI-EAS91_1_306UPAAXX 0 phiX 3093 255 36M * 0 0 GCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAG * +HWI-EAS91_1_306UPAAXX 0 phiX 3151 255 36M * 0 0 GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAG * +HWI-EAS91_1_306UPAAXX 0 phiX 1193 255 36M * 0 0 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC * +HWI-EAS91_1_306UPAAXX 16 phiX 157 255 36M * 0 0 CGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGC * +HWI-EAS91_1_306UPAAXX 0 phiX 4792 255 36M * 0 0 GGACACTTCCTACAGGTAGCGTTGACCCTAATTTTG * +HWI-EAS91_1_306UPAAXX 0 phiX 3849 255 36M * 0 0 GATACTTGGAACAATTTCTGGAAAGACGGTAAAGCT * +HWI-EAS91_1_306UPAAXX 16 phiX 2743 255 1H35M * 0 0 GATGGTGGTTATTATACCGTCAAGGACTGTGTGAC * +HWI-EAS91_1_306UPAAXX 0 phiX 3015 255 36M * 0 0 GCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACC * +HWI-EAS91_1_306UPAAXX 16 phiX 1091 255 36M * 0 0 ATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTC * +HWI-EAS91_1_306UPAAXX 16 phiX 798 255 1H35M * 0 0 GACGCAGAAGAAAACGTGCGTCAAAAATTACGTGC * +HWI-EAS91_1_306UPAAXX 16 phiX 4866 255 36M * 0 0 CAAAATACGTGGCCTTATGGTTACAGTATGCCCATC * +HWI-EAS91_1_306UPAAXX 0 phiX 4404 255 36M * 0 0 GAACGCCCTCTTAAGGATATTCGCGATGAGTATAAT * +HWI-EAS91_1_306UPAAXX 16 phiX 1655 255 36M * 0 0 GTTATTTCTTCATTTGGAGGTAAAACCTCTTATGAC * +HWI-EAS91_1_306UPAAXX 0 phiX 5162 255 36M * 0 0 GCTCACAATGACAAATCTGTCCACGGAGTGCTTAAT * +HWI-EAS91_1_306UPAAXX 16 phiX 1249 255 1H35M * 0 0 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTC * +HWI-EAS91_1_306UPAAXX 0 phiX 3261 255 36M * 0 0 GCTGATAAAGGAAAGGATACTCGTGATTATCTTGCT * +HWI-EAS91_1_306UPAAXX 16 phiX 3096 255 36M * 0 0 ATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCC * +HWI-EAS91_1_306UPAAXX 16 phiX 2812 255 1H35M * 0 0 GTTTATGTTGGTTTCATGGTTTGGTCTAACTTTAC * +HWI-EAS91_1_306UPAAXX 0 phiX 81 255 30M6H * 0 0 GAATTACTACTGCTTGTTTACGAATTAAAT * +HWI-EAS91_1_306UPAAXX 0 phiX 3459 255 36M * 0 0 GCTGGCATTCAGTCGGCGACTTCACGCCAGAATACG * +HWI-EAS91_1_306UPAAXX 0 phiX 789 255 35M1H * 0 0 GTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAA * +HWI-EAS91_1_306UPAAXX 0 phiX 3699 255 36M * 0 0 GCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAAT * +HWI-EAS91_1_306UPAAXX 0 phiX 2641 255 31M5H * 0 0 GCCGACCCTAAATTTTTTGCCTGTTTGGTTC * +HWI-EAS91_1_306UPAAXX 0 phiX 305 255 36M * 0 0 GTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTG * +HWI-EAS91_1_306UPAAXX 0 phiX 3066 255 35M1H * 0 0 GGCATGGGTGATGCTGGTATTAAATCTGCCATTCA * +HWI-EAS91_1_306UPAAXX 0 phiX 4428 255 36M * 0 0 GATGAGTATAATTACCCCAAAAAGAAAGGTATTAAG * +HWI-EAS91_1_306UPAAXX 16 phiX 1461 255 36M * 0 0 AAGATGATGCTCGTTATGGTTTCCGTTGCTGCCATC * +HWI-EAS91_1_306UPAAXX 16 phiX 4000 255 36M * 0 0 CTTCTGAATGTCACGCTGATTATTTTGACTTTGAGC * +HWI-EAS91_1_306UPAAXX 0 phiX 220 255 36M * 0 0 GACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTT * +HWI-EAS91_1_306UPAAXX 0 phiX 3194 255 33M3H * 0 0 GCAGGCTGGCACTTCTGCCGTTTCTGATAAGTT * +HWI-EAS91_1_306UPAAXX 0 phiX 1266 255 32M4H * 0 0 GTGTTAATGCCACTCCTCTCCCGACTGTTAAC * +HWI-EAS91_1_306UPAAXX 16 phiX 4596 255 36M * 0 0 GCTGACGACCGATTAGAGGCGTTTTATGATAATCCC * +HWI-EAS91_1_306UPAAXX 16 phiX 1172 255 1H35M * 0 0 GGCCTTGCTATTGACTCTACTGTAGACATTTTTAC * +HWI-EAS91_1_306UPAAXX 16 phiX 636 255 36M * 0 0 GCTTATTATGTTCATCCCGTCAACATTCAAACGGCC * +HWI-EAS91_1_306UPAAXX 16 phiX 2873 255 36M * 0 0 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC * +HWI-EAS91_1_306UPAAXX 16 phiX 1014 255 36M * 0 0 AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCC * +HWI-EAS91_1_306UPAAXX 0 phiX 2689 255 36M * 0 0 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT * +HWI-EAS91_1_306UPAAXX 0 phiX 2148 255 36M * 0 0 GCGTACTTATTCGCCACCATGATTATTACCAGTGTT * +HWI-EAS91_1_306UPAAXX 0 phiX 5191 255 35M1H * 0 0 GCTTAATCCAACTTACCAAGCTGGGTTACGACGCG * +HWI-EAS91_1_306UPAAXX 16 phiX 1590 255 36M * 0 0 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC * +HWI-EAS91_1_306UPAAXX 0 phiX 571 255 35M1H * 0 0 GTACGCTGTACTTTGTGGGATACCCTCGCTTTCCT * +HWI-EAS91_1_306UPAAXX 0 phiX 242 255 36M * 0 0 GGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGT * +HWI-EAS91_1_306UPAAXX 0 phiX 338 255 36M * 0 0 GTGGATTACTATCTGAGTCCGATGCTGTTCAACCAC * +HWI-EAS91_1_306UPAAXX 0 phiX 1112 255 30M6H * 0 0 GCTGGCGACTCCTTCGAGATGGACGCCGTT * +HWI-EAS91_1_306UPAAXX 16 phiX 1254 255 2H34M * 0 0 TCATGAAGGATGGTGTTAATGCCACTCCTCTCCC * +HWI-EAS91_1_306UPAAXX 0 phiX 3731 255 18M18H * 0 0 GAATCAGCGGTATGGCTC * +HWI-EAS91_1_306UPAAXX 0 phiX 2970 255 36M * 0 0 GCTGGTGGCGCCATGTCTAAATTTTTTGGAGGCGGT * +HWI-EAS91_1_306UPAAXX 0 phiX 5151 255 36M * 0 0 GGGATGAAAATGCTCACAATGACAAATCTGTCCACG * +HWI-EAS91_1_306UPAAXX 16 phiX 348 255 36M * 0 0 ATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAA * +HWI-EAS91_1_306UPAAXX 16 phiX 4888 255 1H35M * 0 0 ACAGTATGCCCATCGCAGTTCGCTACACGCAGGAC * +HWI-EAS91_1_306UPAAXX 0 phiX 3972 255 33M3H * 0 0 GGCTTTTTTATGGTTCGTTCTTATTACCCTTCT * +HWI-EAS91_1_306UPAAXX 0 phiX 5033 255 36M * 0 0 GTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGC * +HWI-EAS91_1_306UPAAXX 16 phiX 1552 255 1H35M * 0 0 GACGACTTCTACCACATCTATTGACATTATGGGTC * +HWI-EAS91_1_306UPAAXX 16 phiX 1764 255 36M * 0 0 AAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAAC * +HWI-EAS91_1_306UPAAXX 0 phiX 1529 255 36M * 0 0 GAGACTGAGCTTTCTCGCCAAATGACGACTTCTACC * +HWI-EAS91_1_306UPAAXX 16 phiX 4950 255 36M * 0 0 CCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACC * +HWI-EAS91_1_306UPAAXX 16 phiX 4484 255 34M2H * 0 0 GGAGGCCTCCACTATGAAATCGCGTAGAGGCTTT * +HWI-EAS91_1_306UPAAXX 16 phiX 4767 255 1H35M * 0 0 CATTTCCATGCGGTGCATTTTATGCGGACACTTCC * +HWI-EAS91_1_306UPAAXX 16 phiX 177 255 36M * 0 0 CTTTGCGACCTTTCGCCATCAACTAACGATTCTGTC * +HWI-EAS91_1_306UPAAXX 0 phiX 2577 255 36M * 0 0 GATGGATACATCTGTCAACGCCGCTAATCAGGTTGT * +HWI-EAS91_1_306UPAAXX 0 phiX 156 255 36M * 0 0 GCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCG * +HWI-EAS91_1_306UPAAXX 0 phiX 227 255 36M * 0 0 TGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGT * +HWI-EAS91_1_306UPAAXX 16 phiX 574 255 36M * 0 0 CGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC * +HWI-EAS91_1_306UPAAXX 0 phiX 3021 255 36M * 0 0 GGTGGCATTCAAGGTGATGTGCTTGCTACCGATAAC * +HWI-EAS91_1_306UPAAXX 16 phiX 3731 255 36M * 0 0 GAATCAGCGGTATGGCTCTTCTCATATTGGCGCTAC * +HWI-EAS91_1_306UPAAXX 0 phiX 2436 255 36M * 0 0 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC * +HWI-EAS91_1_306UPAAXX 0 phiX 2710 255 36M * 0 0 GCCTATGATGTTTATCCTTTGAATGGTCGCCATGAT * +HWI-EAS91_1_306UPAAXX 0 phiX 4228 255 28M8H * 0 0 TTCGTGATGAGTTTGTATCTGTTACTGA * +HWI-EAS91_1_306UPAAXX 0 phiX 4606 255 34M2H * 0 0 GATTAGAGGCGTTTTATGATAATCCCAATGCTTT * +HWI-EAS91_1_306UPAAXX 16 phiX 2766 255 36M * 0 0 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC * +HWI-EAS91_1_306UPAAXX 0 phiX 582 255 34M2H * 0 0 TTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTG * +HWI-EAS91_1_306UPAAXX 16 phiX 664 255 10H26M * 0 0 AAACGGCCTGTCTCATCATGGAAGGC * +HWI-EAS91_1_306UPAAXX 16 phiX 1144 255 36M * 0 0 CGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGC * +HWI-EAS91_1_306UPAAXX 0 phiX 1514 255 36M * 0 0 GCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAA * +HWI-EAS91_1_306UPAAXX 0 phiX 4036 255 26M10H * 0 0 GTATCGAGGCTCTTAAACCTGCTATT * +HWI-EAS91_1_306UPAAXX 16 phiX 1657 255 36M * 0 0 TATTTCTTCATTTGGAGGTAAAACCTCTTATGACGC * +HWI-EAS91_1_306UPAAXX 0 phiX 2071 255 36M * 0 0 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT * +HWI-EAS91_1_306UPAAXX 0 phiX 1626 255 36M * 0 0 GTGATTACTTCATGCAGCGTTACCGTGATGTTATTT * +HWI-EAS91_1_306UPAAXX 0 phiX 3250 255 36M * 0 0 GCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTG * +HWI-EAS91_1_306UPAAXX 0 phiX 1982 255 36M * 0 0 GAAATTTCTATGAATGATGTTTTCCGTTCTGGTGAT * +HWI-EAS91_1_306UPAAXX 16 phiX 2217 255 36M * 0 0 AGGTTAAATTTAATGTGACCGTTTATCGCAATCTGC * +HWI-EAS91_1_306UPAAXX 16 phiX 3486 255 7H29M * 0 0 CAGAATACGAAAGACCAGGTATATGCACA * +HWI-EAS91_1_306UPAAXX 16 phiX 1690 255 36M * 0 0 CGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAA * +HWI-EAS91_1_306UPAAXX 16 phiX 3252 255 7H29M * 0 0 AAGTCTGCCGATGATAAAGGAAAGGATAC * +HWI-EAS91_1_306UPAAXX 16 phiX 1082 255 36M * 0 0 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC * +HWI-EAS91_1_306UPAAXX 16 phiX 4282 255 36M * 0 0 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA * +HWI-EAS91_1_306UPAAXX 0 phiX 4136 255 34M2H * 0 0 GCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAG * +HWI-EAS91_1_306UPAAXX 0 phiX 4414 255 36M * 0 0 TTAAGGATATTCGCGATGAGTATAATTACCCCAAAA * +HWI-EAS91_1_306UPAAXX 16 phiX 3988 255 36M * 0 0 GTTCTTATTACCCTTCTGAATGTCACGCTGATTATT * +HWI-EAS91_1_306UPAAXX 0 phiX 4203 255 36M * 0 0 GACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAG * +HWI-EAS91_1_306UPAAXX 16 phiX 4098 255 36M * 0 0 ATGCTTGGCTTCAATAAGCAGATGGATAACCGCATC * +HWI-EAS91_1_306UPAAXX 0 phiX 4307 255 35M1H * 0 0 TATTAATAACACTATAGACCACCGCCCCGAAGGGG * +HWI-EAS91_1_306UPAAXX 0 phiX 1521 255 35M1H * 0 0 TTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACG * +HWI-EAS91_1_306UPAAXX 16 phiX 945 255 36M * 0 0 TAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGC * +HWI-EAS91_1_306UPAAXX 16 phiX 1575 255 36M * 0 0 ACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGC * +HWI-EAS91_1_306UPAAXX 0 phiX 2689 255 36M * 0 0 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT * +HWI-EAS91_1_306UPAAXX 0 phiX 2099 255 36M * 0 0 GAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGT * +HWI-EAS91_1_306UPAAXX 16 phiX 524 255 36M * 0 0 TACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGC * +HWI-EAS91_1_306UPAAXX 0 phiX 1252 255 36M * 0 0 GTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCC * +HWI-EAS91_1_306UPAAXX 0 phiX 4916 255 33M3H * 0 0 GCAGGACGCTTTTTCACGTTCTGGTTGGTTGTG * +HWI-EAS91_1_306UPAAXX 0 phiX 3828 255 29M7H * 0 0 GGTATTGATAAAGCTGTTGCCGATACTTG * +HWI-EAS91_1_306UPAAXX 16 phiX 3756 255 36M * 0 0 ATTGGCGCTACTGCAAAGGATATTTCTAATGTCGTC * +HWI-EAS91_1_306UPAAXX 16 phiX 664 255 10H26M * 0 0 AAACGGCCTGTCTCATCATGGAAGGC * +HWI-EAS91_1_306UPAAXX 16 phiX 1116 255 36M * 0 0 GCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCC * +HWI-EAS91_1_306UPAAXX 16 phiX 3099 255 36M * 0 0 CAAGGCTCTAATGTTCCTAACCCTGATGGGGCCGCC * +HWI-EAS91_1_306UPAAXX 16 phiX 3082 255 36M * 0 0 GTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTA * +HWI-EAS91_1_306UPAAXX 0 phiX 4221 255 27M9H * 0 0 TCTGACGTTCGTGATGAGTTTGTATCT * +HWI-EAS91_1_306UPAAXX 16 phiX 613 255 36M * 0 0 TTGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC * +HWI-EAS91_1_306UPAAXX 16 phiX 4936 255 36M * 0 0 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC * +HWI-EAS91_1_306UPAAXX 0 phiX 289 255 36M * 0 0 TCACATTTTGTTCATGGTAGAGATTCTCTTGTTGAC * +HWI-EAS91_1_306UPAAXX 0 phiX 4690 255 36M * 0 0 GCAAGGCTAATGATTCACACGCCGACTGCTATCAGT * +HWI-EAS91_1_306UPAAXX 0 phiX 2547 255 31M5H * 0 0 TGGTAATGGTGGTTTTCTTCATTTCATTCAG * +HWI-EAS91_1_306UPAAXX 16 phiX 3520 255 36M * 0 0 AGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTC * +HWI-EAS91_1_306UPAAXX 16 phiX 3858 255 36M * 0 0 AACAATTTCTGGAAAGACGGTAAAGCTGATGGTATT * +HWI-EAS91_1_306UPAAXX 16 phiX 3344 255 36M * 0 0 TTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAA * +HWI-EAS91_1_306UPAAXX 0 phiX 4672 255 27M9H * 0 0 TTCTTGCTGCCGAGGGTCGCAAGGCTA * +HWI-EAS91_1_306UPAAXX 0 phiX 1346 255 31M5H * 0 0 GATACCAATAAAATCCCTAAGCATTTGTTTC * +HWI-EAS91_1_306UPAAXX 16 phiX 1800 255 36M * 0 0 AGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTC * +HWI-EAS91_1_306UPAAXX 0 phiX 5077 255 36M * 0 0 GGAACAACTCACTAAAAACCAAGCTGTCGCTACTTC * +HWI-EAS91_1_306UPAAXX 16 phiX 2763 255 1H35M * 0 0 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA * +HWI-EAS91_1_306UPAAXX 0 phiX 4282 255 36M * 0 0 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA * +HWI-EAS91_1_306UPAAXX 0 phiX 4656 255 27M9H * 0 0 GATATTGGTCGTATGGTTCTTGCTGCC * +HWI-EAS91_1_306UPAAXX 0 phiX 4935 255 18M18H * 0 0 TCTGGTTGGTTGTGGCCT * +HWI-EAS91_1_306UPAAXX 0 phiX 1203 255 33M3H * 0 0 TTACTTTTTATGTCCCTCATCGTCACGTTTATG * +HWI-EAS91_1_306UPAAXX 16 phiX 5107 255 36M * 0 0 TACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCC * +HWI-EAS91_1_306UPAAXX 16 phiX 5168 255 1H35M * 0 0 AATGACAAATCTGTCCACGGAGTGCTTAATCCAAC * +HWI-EAS91_1_306UPAAXX 0 phiX 2956 255 36M * 0 0 TTGCTTCTGCTCTTGCTTGTGGCGCCATGTCTAAAT * +HWI-EAS91_1_306UPAAXX 0 phiX 624 255 30M6H * 0 0 GCTGCCGTCATTGCTTATTATGTTCATCCC * +HWI-EAS91_1_306UPAAXX 16 phiX 1877 255 1H35M * 0 0 CCGCCTACTGCGACTAAAGAGATTCAGTACCTTAA * +HWI-EAS91_1_306UPAAXX 0 phiX 1214 255 36M * 0 0 GTCCCTCATCGTCACGTTTATGGTGAACAGTGGATT * +HWI-EAS91_1_306UPAAXX 0 phiX 558 255 27M9H * 0 0 GCTTGCGTTTATGGTACGCTGGACTTT * +HWI-EAS91_1_306UPAAXX 16 phiX 3629 255 36M * 0 0 TACTCAAGCTCAAACGGCTGGTCAGTATTTTACCAA * +HWI-EAS91_1_306UPAAXX 0 phiX 2254 255 35M1H * 0 0 GACCACTCGCGATTCAATCATGACTTCGTGATAAA * +HWI-EAS91_1_306UPAAXX 0 phiX 2479 255 36M * 0 0 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA * +HWI-EAS91_1_306UPAAXX 0 phiX 3001 255 36M * 0 0 GCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTG * +HWI-EAS91_1_306UPAAXX 0 phiX 4667 255 35M1H * 0 0 TATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATG * +HWI-EAS91_1_306UPAAXX 16 phiX 3464 255 36M * 0 0 CATTCAGTCGGCGACTTCACGCCAGAATACGAAAGA * +HWI-EAS91_1_306UPAAXX 0 phiX 4911 255 35M1H * 0 0 TACACGCAGGACGCTTTTTCACGTTCTGGTTGGTT * +HWI-EAS91_1_306UPAAXX 0 phiX 4592 255 29M7H * 0 0 GTTGGCTGACGACCGATTAGAGGCGTTTT * +HWI-EAS91_1_306UPAAXX 16 phiX 2222 255 36M * 0 0 AAATTTAATGTGAACGTTTATCGCAATCTGCCGACC * +HWI-EAS91_1_306UPAAXX 0 phiX 646 255 35M1H * 0 0 TTCATCCCGTCAACATTCAAACGGCCTGTCTCATC * +HWI-EAS91_1_306UPAAXX 16 phiX 1364 255 36M * 0 0 AAGCATTTGTTTCAGGGTTATTTGAATATCTATAAC * +HWI-EAS91_1_306UPAAXX 16 phiX 1613 255 1H35M * 0 0 ACTGACCAAGAACGTGATTACTTCATGCAGCGTTA * +HWI-EAS91_1_306UPAAXX 0 phiX 1045 255 35M1H * 0 0 TTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTC * +HWI-EAS91_1_306UPAAXX 0 phiX 2885 255 33M3H * 0 0 TTATTAAAGAGATTATTTTTCTCCAGCCACTTA * +HWI-EAS91_1_306UPAAXX 0 phiX 1588 255 36M * 0 0 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA * +HWI-EAS91_1_306UPAAXX 0 phiX 339 255 36M * 0 0 TGGATTACTATCTGAGTCCGATGCTGTTCAACCACT * +HWI-EAS91_1_306UPAAXX 0 phiX 4937 255 36M * 0 0 TGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCC * +HWI-EAS91_1_306UPAAXX 16 phiX 4344 255 2H34M * 0 0 GAAAAATGGTTTTTAGAGAACGAGAAGACGGTTA * +HWI-EAS91_1_306UPAAXX 16 phiX 3674 255 36M * 0 0 CAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGA * +HWI-EAS91_1_306UPAAXX 0 phiX 4945 255 36M * 0 0 TGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAG * +HWI-EAS91_1_306UPAAXX 0 phiX 1946 255 36M * 0 0 GGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGT * +HWI-EAS91_1_306UPAAXX 0 phiX 2662 255 32M4H * 0 0 TGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCC * +HWI-EAS91_1_306UPAAXX 0 phiX 3313 255 36M * 0 0 TTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCT * +HWI-EAS91_1_306UPAAXX 16 phiX 2827 255 3H33M * 0 0 ATGGTTTGGTCAAACTTTACCGCTACTAAATGC * +HWI-EAS91_1_306UPAAXX 0 phiX 727 255 36M * 0 0 GTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCT * +HWI-EAS91_1_306UPAAXX 16 phiX 555 255 36M * 0 0 GAGGCTTGCGTTTATGGTACGCTGGACTTTGTGGGA * +HWI-EAS91_1_306UPAAXX 0 phiX 485 255 36M * 0 0 TGATTTCGATTTTCTGACGAGTAACAAAGTTTGGAT * +HWI-EAS91_1_306UPAAXX 16 phiX 318 255 36M * 0 0 TGTTGACATTTTAAAAGAGCGTGGATTACTATCTGA * +HWI-EAS91_1_306UPAAXX 0 phiX 910 255 36M * 0 0 TAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGT * +HWI-EAS91_1_306UPAAXX 16 phiX 4936 255 36M * 0 0 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC * +HWI-EAS91_1_306UPAAXX 16 phiX 5005 255 36M * 0 0 TCTATGTGGCTAAATACGTTAACAAAAAGTCAGATA * +HWI-EAS91_1_306UPAAXX 0 phiX 1379 255 33M3H * 0 0 GGTTATTTGAATATCTATAACAACTATTTTAAA * +HWI-EAS91_1_306UPAAXX 0 phiX 3168 255 30M6H * 0 0 GGTAAAGGACTTCTTGAAGGTACGTTGCAG * +HWI-EAS91_1_306UPAAXX 0 phiX 2738 255 33M3H * 0 0 GCCATGATGGTGGTTATTATACCGTCAAGGACT * +HWI-EAS91_1_306UPAAXX 0 phiX 4099 255 36M * 0 0 TGCTTGGCTTCCATAAGCAGATGGATAACCGCATCA * +HWI-EAS91_1_306UPAAXX 0 phiX 2574 255 36M * 0 0 TCAGATGGATACATCTGTCAACGCCGCTAATCAGGT * +HWI-EAS91_1_306UPAAXX 0 phiX 2453 255 36M * 0 0 TCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTT * +HWI-EAS91_1_306UPAAXX 0 phiX 5179 255 36M * 0 0 TGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGG * +HWI-EAS91_1_306UPAAXX 0 phiX 3778 255 30M6H * 0 0 TTTCTAATGTCGTCACTGATGCTGCTTCTG * +HWI-EAS91_1_306UPAAXX 16 phiX 1843 255 36M * 0 0 TGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCC * +HWI-EAS91_1_306UPAAXX 16 phiX 1082 255 36M * 0 0 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC * +HWI-EAS91_1_306UPAAXX 16 phiX 1728 255 36M * 0 0 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC * +HWI-EAS91_1_306UPAAXX 0 phiX 4637 255 36M * 0 0 TTTGCGTGACTATTTTCGTGATATTGTTCGTATGGT * +HWI-EAS91_1_306UPAAXX 0 phiX 2225 255 36M * 0 0 TTTAATGTGACCGTTTATCGCAATCTGCCGACCACT * +HWI-EAS91_1_306UPAAXX 16 phiX 3368 255 36M * 0 0 CGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCA * +HWI-EAS91_1_306UPAAXX 0 phiX 3311 255 36M * 0 0 GCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTC * +HWI-EAS91_1_306UPAAXX 16 phiX 5039 255 36M * 0 0 TATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGA * +HWI-EAS91_1_306UPAAXX 0 phiX 2059 255 36M * 0 0 TTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCT * +HWI-EAS91_1_306UPAAXX 0 phiX 388 255 36M * 0 0 TCATGAGTCAAGTTACTGAACAATCCGTACGTTTCC * +HWI-EAS91_1_306UPAAXX 0 phiX 2754 255 36M * 0 0 TTATACCGTCAAGGACTGTGTGACTATTGACGTCCT * +HWI-EAS91_1_306UPAAXX 16 phiX 2128 255 36M * 0 0 TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCA * +HWI-EAS91_1_306UPAAXX 0 phiX 154 255 36M * 0 0 TTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTT * +HWI-EAS91_1_306UPAAXX 16 phiX 3617 255 36M * 0 0 GCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTCA * +HWI-EAS91_1_306UPAAXX 16 phiX 2728 255 36M * 0 0 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC * +HWI-EAS91_1_306UPAAXX 16 phiX 2775 255 36M * 0 0 GACTATTGACGTCCTTCCCCGTACGCCGGGCAATAA * +HWI-EAS91_1_306UPAAXX 0 phiX 4217 255 36M * 0 0 TGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTAC * +HWI-EAS91_1_306UPAAXX 0 phiX 4619 255 35M1H * 0 0 TTATGATAATCCCAATGCTTTGCGTGACTATTTTC * +HWI-EAS91_1_306UPAAXX 0 phiX 411 255 35M1H * 0 0 TCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAA * +HWI-EAS91_1_306UPAAXX 0 phiX 2730 255 35M1H * 0 0 GAATGGTCGCCATGATGGTGGTTATTATACCGTCA * +HWI-EAS91_1_306UPAAXX 0 phiX 521 255 35M1H * 0 0 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTG * +HWI-EAS91_1_306UPAAXX 0 phiX 4260 255 36M * 0 0 TTAATGGATGAATTGGCACAATGCTACAATGTGCTC * +HWI-EAS91_1_306UPAAXX 16 phiX 1662 255 36M * 0 0 CTTCATTTGGAGGTAAAACCTCTTATGACGCTGACA * +HWI-EAS91_1_306UPAAXX 16 phiX 533 255 36M * 0 0 CTCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGTTTA * +HWI-EAS91_1_306UPAAXX 0 phiX 195 255 35M1H * 0 0 TCAACTAACGATTCTGTCAAAAACTGACGCGTTGG * +HWI-EAS91_1_306UPAAXX 0 phiX 2239 255 36M * 0 0 TTATCGCAATCTGCCGACCACTCGCGATTCAATCAT * +HWI-EAS91_1_306UPAAXX 0 phiX 1313 255 33M3H * 0 0 GACCATGCCGCTTTTCTTGGCACGATTAACCCT * +HWI-EAS91_1_306UPAAXX 16 phiX 1545 255 36M * 0 0 GCCAAATGACGACTTCTACCACATCTATTGACATTA * +HWI-EAS91_1_306UPAAXX 16 phiX 557 255 36M * 0 0 GGCTTGCGTTTATGGTACGCTGGACTTTGTGGGATA * +HWI-EAS91_1_306UPAAXX 0 phiX 1249 255 36M * 0 0 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCT * +HWI-EAS91_1_306UPAAXX 0 phiX 4174 255 33M3H * 0 0 TTGAGTTCGATAATGGTGATATGTATGTTGACG * +HWI-EAS91_1_306UPAAXX 0 phiX 614 255 35M1H * 0 0 TGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC * +HWI-EAS91_1_306UPAAXX 0 phiX 206 255 36M * 0 0 TTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGT * +HWI-EAS91_1_306UPAAXX 16 phiX 4981 255 36M * 0 0 CTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTA * +HWI-EAS91_1_306UPAAXX 0 phiX 1702 255 30M6H * 0 0 TCCTTTACTTGTCATGCGCTCTAATCTCTG * +HWI-EAS91_1_306UPAAXX 0 phiX 2321 255 35M1H * 0 0 TAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACC * +HWI-EAS91_1_306UPAAXX 0 phiX 4184 255 35M1H * 0 0 TAATGGTGATATGTATGTTTACGTCCATAAGGCTG * +HWI-EAS91_1_306UPAAXX 0 phiX 4088 255 35M1H * 0 0 TCAATCCCCAATGCTTGGCTTCCATAAGCAGATGG * +HWI-EAS91_1_306UPAAXX 0 phiX 900 255 36M * 0 0 TGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTC * +HWI-EAS91_1_306UPAAXX 16 phiX 1155 255 36M * 0 0 TTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTA * +HWI-EAS91_1_306UPAAXX 16 phiX 3587 255 36M * 0 0 TTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCA * +HWI-EAS91_1_306UPAAXX 16 phiX 3787 255 36M * 0 0 TCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTT * +HWI-EAS91_1_306UPAAXX 0 phiX 3834 255 35M1H * 0 0 GATAAAGCTGTTGCCGATACTTGGAACAATTTCTG * +HWI-EAS91_1_306UPAAXX 0 phiX 989 255 36M * 0 0 TGAGGATAAATTATGTCTAATATTCAAACTGGCGCC * +HWI-EAS91_1_306UPAAXX 0 phiX 5358 255 29M7H * 0 0 ATAAAAATGATTGGCGTATCCAACCTGCA * +HWI-EAS91_1_306UPAAXX 0 phiX 3712 255 36M * 0 0 TAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCT * +HWI-EAS91_1_306UPAAXX 0 phiX 1207 255 36M * 0 0 TTTTTATGTCCCTCATCGTCACGTTTATGGTGAACA * +HWI-EAS91_1_306UPAAXX 16 phiX 4300 255 15H21M * 0 0 AACTTGATATTAATAACACTA * +HWI-EAS91_1_306UPAAXX 0 phiX 2116 255 36M * 0 0 TCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGT * +HWI-EAS91_1_306UPAAXX 16 phiX 660 255 36M * 0 0 ATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAA * +HWI-EAS91_1_306UPAAXX 0 phiX 1807 255 31M5H * 0 0 TAAACATTCTGTGCCGCGTTTCTTTGTTCCT * +HWI-EAS91_1_306UPAAXX 16 phiX 1536 255 36M * 0 0 AGCTTTCTCGCCAAATGACGACTTCTACCACATCTA * +HWI-EAS91_1_306UPAAXX 16 phiX 2112 255 1H35M * 0 0 TCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAA * +HWI-EAS91_1_306UPAAXX 16 phiX 2816 255 36M * 0 0 ATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTA * +HWI-EAS91_1_306UPAAXX 0 phiX 5176 255 36M * 0 0 ATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGC * +HWI-EAS91_1_306UPAAXX 16 phiX 3472 255 36M * 0 0 CGGCGACTTCACGCCAGAATACGAAAGACCAGGTAT * +HWI-EAS91_1_306UPAAXX 16 phiX 1948 255 36M * 0 0 CGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGA * +HWI-EAS91_1_306UPAAXX 0 phiX 2212 255 35M1H * 0 0 TAGTCAGGTTAAATTTAATGTGACCGTTTATCGCA * +HWI-EAS91_1_306UPAAXX 0 phiX 3817 255 35M1H * 0 0 ATATTTTTCATGGTATTGATAAAGCTGTTGCCGAT * +HWI-EAS91_1_306UPAAXX 16 phiX 3563 255 36M * 0 0 GTCTATTATGGAAAACACCAATCTTTCCAAGCAACA * +HWI-EAS91_1_306UPAAXX 16 phiX 4198 255 1H35M * 0 0 ATGTTGACGGCCATAAGGCTGCTTCTGACGTTCGT * +HWI-EAS91_1_306UPAAXX 16 phiX 2907 255 1H35M * 0 0 CCAGCCACTTAAGTGAGGTGATTTATGTTTGGTGC * +HWI-EAS91_1_306UPAAXX 0 phiX 2843 255 35M1H * 0 0 TTACCGCTACTAAATGCCGCGGATTGGTTTCGCTG * +HWI-EAS91_1_306UPAAXX 0 phiX 4374 255 31M5H * 0 0 GTTACGCAGTTTTGCCGCAAGCTGGCTGCTG * +HWI-EAS91_1_306UPAAXX 16 phiX 424 255 2H24M10H * 0 0 AGACCGCCTTGGCCTCTATTAAGC * +HWI-EAS91_1_306UPAAXX 16 phiX 1940 255 1H35M * 0 0 ATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCC * +HWI-EAS91_1_306UPAAXX 16 phiX 5073 255 36M * 0 0 GAATGGAACAACTCACTAAAAACCAAGCTGTCGCTA * +HWI-EAS91_1_306UPAAXX 0 phiX 4434 255 36M * 0 0 TATAATTACCCCAAAAAGAAAGGTATTAAGGATGAG * +HWI-EAS91_1_306UPAAXX 16 phiX 1274 255 1H35M * 0 0 GCCACTCCTCTCCCGACTGTTAACACTACTGGTTA * +HWI-EAS91_1_306UPAAXX 0 phiX 4440 255 36M * 0 0 TACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCA * +HWI-EAS91_1_306UPAAXX 0 phiX 3920 255 36M * 0 0 GTCAGGATTGACACCCTCCCAATTGTATGTTTTCAT * +HWI-EAS91_1_306UPAAXX 16 phiX 4587 255 1H35M * 0 0 CTCACGTTGGCTGACGACCGATTAGAGGCGTTTTA * +HWI-EAS91_1_306UPAAXX 0 phiX 3730 255 24M12H * 0 0 AGAATCAGCGGTATGGCTCTTCTC * +HWI-EAS91_1_306UPAAXX 0 phiX 1707 255 36M * 0 0 TACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCT * +HWI-EAS91_1_306UPAAXX 16 phiX 1582 255 36M * 0 0 GGGTCTGCAAGCTGCTTATGCTAATTTGCATACTGA * +HWI-EAS91_1_306UPAAXX 16 phiX 1588 255 36M * 0 0 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA * +HWI-EAS91_1_306UPAAXX 0 phiX 1180 255 34M2H * 0 0 TATTGACTCTACTGTAGACATTTTTACTTTTTAT * +HWI-EAS91_1_306UPAAXX 16 phiX 2698 255 36M * 0 0 ACCCTCCCGACTGCCTATGATGTTTATCCTTTGAAT * +HWI-EAS91_1_306UPAAXX 0 phiX 3167 255 36M * 0 0 TGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGG * +HWI-EAS91_1_306UPAAXX 16 phiX 2498 255 11H25M * 0 0 CTAAAGCTACATCGTCAACGTTATA * +HWI-EAS91_1_306UPAAXX 16 phiX 2289 255 36M * 0 0 AGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAA * +HWI-EAS91_1_306UPAAXX 16 phiX 441 255 36M * 0 0 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA * +HWI-EAS91_1_306UPAAXX 0 phiX 1012 255 36M * 0 0 TCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTC * +HWI-EAS91_1_306UPAAXX 0 phiX 161 255 36M * 0 0 GCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATC * +HWI-EAS91_1_306UPAAXX 16 phiX 1165 255 1H35M * 0 0 GCGTCGTGGCCTTGCTATTGACTCTACTGTAGACA * +HWI-EAS91_1_306UPAAXX 16 phiX 5015 255 5H31M * 0 0 TAAATACGTTAACAAAAAGTCAGATATGGAC * +HWI-EAS91_1_306UPAAXX 16 phiX 2286 255 36M * 0 0 AAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGT * +HWI-EAS91_1_306UPAAXX 16 phiX 801 255 32M4H * 0 0 GCAGAAGAAAACGTGCGTCAAAAATTACGTGC * +HWI-EAS91_1_306UPAAXX 0 phiX 1587 255 36M * 0 0 TGCAAGCTGCTTATGCTAATTTGCATACTGACCAAG * +HWI-EAS91_1_306UPAAXX 0 phiX 4578 255 36M * 0 0 TTTGACACTCTCACGTTGGCTGACGACCGATTAGAG * +HWI-EAS91_1_306UPAAXX 16 phiX 3570 255 36M * 0 0 ATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTT * +HWI-EAS91_1_306UPAAXX 16 phiX 496 255 36M * 0 0 TTCTGACGAGTAACAAAGTTTGGATTGCTACTGACC * +HWI-EAS91_1_306UPAAXX 0 phiX 441 255 36M * 0 0 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA * +HWI-EAS91_1_306UPAAXX 16 phiX 4754 255 30M6H * 0 0 TAATGGCCGTCTTCATTTCCATGCGGTGCA * +HWI-EAS91_1_306UPAAXX 0 phiX 4235 255 35M1H * 0 0 TGAGTTTGTATCTGTTACTGAGAAGTTAATGGATG * +HWI-EAS91_1_306UPAAXX 16 phiX 2293 255 36M * 0 0 TGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAATT * +HWI-EAS91_1_306UPAAXX 16 phiX 1579 255 1H35M * 0 0 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATA * +HWI-EAS91_1_306UPAAXX 0 phiX 3332 255 31M5H * 0 0 TGGTGCTGATGCTTCCTCTGCTGGTATGGTT * +HWI-EAS91_1_306UPAAXX 0 phiX 4132 255 36M * 0 0 TCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGC * +HWI-EAS91_1_306UPAAXX 0 phiX 4775 255 36M * 0 0 TGCGGTGCATTTTATGCGGACACTTCCTACAGGTAG * +HWI-EAS91_1_306UPAAXX 16 phiX 657 255 18H18M * 0 0 AACATTCAAACGGCCTGT * +HWI-EAS91_1_306UPAAXX 16 phiX 1 255 4H32M * 0 0 GAGTTTTATCGCTTCCATGACGCAGAAGTTAA * +HWI-EAS91_1_306UPAAXX 16 phiX 54 255 36M * 0 0 AGTCGAAAAATTATCTTGATAAAGCAGGAATTACTA * +HWI-EAS91_1_306UPAAXX 16 phiX 680 255 1H35M * 0 0 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA * +HWI-EAS91_1_306UPAAXX 0 phiX 231 255 36M * 0 0 TGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGT * +HWI-EAS91_1_306UPAAXX 0 phiX 4192 255 36M * 0 0 ATATGTATGTTGACGGCCATAAGGCTGCTTCTGACG * +HWI-EAS91_1_306UPAAXX 16 phiX 1580 255 36M * 0 0 ATGGGTCTGCAAGCTGCTTATGCTAATTTGCATACT * +HWI-EAS91_1_306UPAAXX 16 phiX 611 255 36M * 0 0 TGTTGAGTTTATTGCTGCCGTCATTGCTTATTATGT * +HWI-EAS91_1_306UPAAXX 16 phiX 4532 255 1H35M * 0 0 GATGAATGCAATGCGACAGGCTCATGCTGATGGTT * +HWI-EAS91_1_306UPAAXX 0 phiX 638 255 30M6H * 0 0 TTATTATGTTCATCCCGTCAACATTCAAAC * +HWI-EAS91_1_306UPAAXX 0 phiX 2847 255 4H28M4H * 0 0 CGCTACTAAATTCCGCGGATTGGTTTCG * +HWI-EAS91_1_306UPAAXX 16 phiX 1957 255 36M * 0 0 TTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTAT * +HWI-EAS91_1_306UPAAXX 16 phiX 2728 255 36M * 0 0 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC * +HWI-EAS91_1_306UPAAXX 0 phiX 3979 255 36M * 0 0 TTATGGTTCGTTCTTATTACCCTTCTGAATGTCACG * +HWI-EAS91_1_306UPAAXX 16 phiX 540 255 1H35M * 0 0 GCTCGTCGCTGCGTTGAGGCTTGCGTTTATGGTAC * +HWI-EAS91_1_306UPAAXX 16 phiX 1852 255 36M * 0 0 GTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGC * +HWI-EAS91_1_306UPAAXX 16 phiX 4684 255 36M * 0 0 AGGGTCGCAAGGCTAATGATTCACACGCCGACTGCT * +HWI-EAS91_1_306UPAAXX 0 phiX 3690 255 32M4H * 0 0 AAGGTTAGTGCTGAGGTTGACTTAGTTCATCA * +HWI-EAS91_1_306UPAAXX 0 phiX 584 255 36M * 0 0 TGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTT * +HWI-EAS91_1_306UPAAXX 16 phiX 3852 255 1H35M * 0 0 ACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGA * +HWI-EAS91_1_306UPAAXX 0 phiX 2857 255 35M1H * 0 0 TGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAA * +HWI-EAS91_1_306UPAAXX 0 phiX 3782 255 26M10H * 0 0 TAATGTCGTCACTGATGCTGCTTCTG * +HWI-EAS91_1_306UPAAXX 0 phiX 1728 255 36M * 0 0 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC * +HWI-EAS91_1_306UPAAXX 0 phiX 4658 255 35M1H * 0 0 TATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCA * +HWI-EAS91_1_306UPAAXX 16 phiX 1721 255 36M * 0 0 TCTAATCTCTGGGCATCTGGCTATGATGTTGATGGA * +HWI-EAS91_1_306UPAAXX 0 phiX 1954 255 36M * 0 0 TGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTC * +HWI-EAS91_1_306UPAAXX 0 phiX 962 255 36M * 0 0 TAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAA * +HWI-EAS91_1_306UPAAXX 16 phiX 2175 255 36M * 0 0 ACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGA * +HWI-EAS91_1_306UPAAXX 0 phiX 2442 255 36M * 0 0 TAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTC * +HWI-EAS91_1_306UPAAXX 16 phiX 5126 255 1H35M * 0 0 CAGAATCAGAATGAGCCGCAACTTCGGGATGAAAA * +HWI-EAS91_1_306UPAAXX 16 phiX 5206 255 36M * 0 0 CCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGA * +HWI-EAS91_1_306UPAAXX 0 phiX 2879 255 36M * 0 0 ATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC * +HWI-EAS91_1_306UPAAXX 0 phiX 2004 255 36M * 0 0 TCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTG * +HWI-EAS91_1_306UPAAXX 0 phiX 2154 255 36M * 0 0 TTATTCGCCACCATGATTATGACCAGTGTTTCCAGT * +HWI-EAS91_1_306UPAAXX 0 phiX 1093 255 35M1H * 0 0 TTCAACTACTCCGGTTATCGCTGGCGACTCCTTCG * +HWI-EAS91_1_306UPAAXX 16 phiX 3127 255 36M * 0 0 AGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGCTA * +HWI-EAS91_1_306UPAAXX 16 phiX 3544 255 36M * 0 0 AGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACA * +HWI-EAS91_1_306UPAAXX 0 phiX 2508 255 31M5H * 0 0 ATCGTCAACGTTATATTTTGATAGTTTGACG * +HWI-EAS91_1_306UPAAXX 16 phiX 3098 255 12H24M * 0 0 TCAAGGCTCTAATGTCCCTAACCC * +HWI-EAS91_1_306UPAAXX 16 phiX 3600 255 36M * 0 0 CAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAA * +HWI-EAS91_1_306UPAAXX 16 phiX 1734 255 36M * 0 0 CATCTGGCTATGATGTTGATGGAACTGACCAAACGT * +HWI-EAS91_1_306UPAAXX 0 phiX 2655 255 33M3H * 0 0 TTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTC * +HWI-EAS91_1_306UPAAXX 16 phiX 2934 255 36M * 0 0 TTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTT * +HWI-EAS91_1_306UPAAXX 0 phiX 1522 255 36M * 0 0 TCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGAC * +HWI-EAS91_1_306UPAAXX 16 phiX 4063 255 36M * 0 0 AGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAA * +HWI-EAS91_1_306UPAAXX 0 phiX 3395 255 36M * 0 0 TAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA * +HWI-EAS91_1_306UPAAXX 0 phiX 409 255 36M * 0 0 AATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTA * +HWI-EAS91_1_306UPAAXX 16 phiX 1867 255 36M * 0 0 TGTTCGTTTTCCGCCTACTGCGACTAAAGAGATTCA * +HWI-EAS91_1_306UPAAXX 0 phiX 3780 255 33M3H * 0 0 TCTAATGTCGTCACTGATGCTGCTTCTGGTGTG * +HWI-EAS91_1_306UPAAXX 0 phiX 3982 255 36M * 0 0 TGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTG * +HWI-EAS91_1_306UPAAXX 16 phiX 2815 255 36M * 0 0 TATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCT * +HWI-EAS91_1_306UPAAXX 16 phiX 1242 255 36M * 0 0 AGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCA * +HWI-EAS91_1_306UPAAXX 16 phiX 4659 255 1H35M * 0 0 ATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAA * +HWI-EAS91_1_306UPAAXX 0 phiX 2436 255 36M * 0 0 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC * +HWI-EAS91_1_306UPAAXX 0 phiX 527 255 35M1H * 0 0 TGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTT * +HWI-EAS91_1_306UPAAXX 16 phiX 4817 255 36M * 0 0 CCCTAATTTGGGTCGTCGGGTACGCAATCGCCGCCA * +HWI-EAS91_1_306UPAAXX 0 phiX 2100 255 36M * 0 0 AAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTG * +HWI-EAS91_1_306UPAAXX 16 phiX 2762 255 36M * 0 0 TCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA * +HWI-EAS91_1_306UPAAXX 0 phiX 4955 255 36M * 0 0 TGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTA * +HWI-EAS91_1_306UPAAXX 16 phiX 3761 255 36M * 0 0 CGCTACTGCAAAGGATATTTCTAATGTCGTCACTGA * +HWI-EAS91_1_306UPAAXX 16 phiX 4499 255 36M * 0 0 GAAATCGCGTAGAGGCTTTACTATTCAGCGTTTGAT * +HWI-EAS91_1_306UPAAXX 16 phiX 4039 255 36M * 0 0 TCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCA * +HWI-EAS91_1_306UPAAXX 16 phiX 4788 255 36M * 0 0 ATGCGGACACTTCCTACAGGTAGCGTTGACCCTAAT * +HWI-EAS91_1_306UPAAXX 0 phiX 1002 255 36M * 0 0 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC * +HWI-EAS91_1_306UPAAXX 16 phiX 4381 255 36M * 0 0 AGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTA * +HWI-EAS91_1_306UPAAXX 16 phiX 4256 255 36M * 0 0 GAAGTTAATGGATGAATTGGCACAATGCTACAATGT * +HWI-EAS91_1_306UPAAXX 16 phiX 10 255 1H35M * 0 0 CGCTTCCATGACGCAGAAGTTAACACTTTCGGATA * +HWI-EAS91_1_306UPAAXX 16 phiX 1579 255 36M * 0 0 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATAC * +HWI-EAS91_1_306UPAAXX 0 phiX 989 255 30M6H * 0 0 TGAGGATAAATTATGTCTAATATTCAAACT * +HWI-EAS91_1_306UPAAXX 0 phiX 3862 255 36M * 0 0 ATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCT * +HWI-EAS91_1_306UPAAXX 0 phiX 3278 255 36M * 0 0 TACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCT * +HWI-EAS91_1_306UPAAXX 0 phiX 349 255 36M * 0 0 TCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAG * +HWI-EAS91_1_306UPAAXX 0 phiX 4608 255 36M * 0 0 TTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGT * +HWI-EAS91_1_306UPAAXX 0 phiX 3808 255 35M1H * 0 0 GTGTGGTTGATATTTTTCATGGTATTGATAAAGCT * +HWI-EAS91_1_306UPAAXX 16 phiX 4753 255 31M5H * 0 0 CTAATGGCCGTCTTCATTTCCATGCGGTGCA * +HWI-EAS91_1_306UPAAXX 16 phiX 286 255 36M * 0 0 GAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTT * +HWI-EAS91_1_306UPAAXX 0 phiX 2850 255 36M * 0 0 TACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGT * +HWI-EAS91_1_306UPAAXX 16 phiX 680 255 1H35M * 0 0 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA * +HWI-EAS91_1_306UPAAXX 16 phiX 4740 255 36M * 0 0 GAGTATGGTACAGCTAATGGCCGTCTTCATTTCCAT * +HWI-EAS91_1_306UPAAXX 0 phiX 4655 255 25M11H * 0 0 TGATATTGGTCGTATGGTTCTTGCT * +HWI-EAS91_1_306UPAAXX 16 phiX 2467 255 1H35M * 0 0 CCAGCTTCTTCGGCACCTGTTTTACAGACACCTAA * +HWI-EAS91_1_306UPAAXX 0 phiX 320 255 34M2H * 0 0 TTGACATTTTAAAAGAGCGTGGATTACTATCTGA * +HWI-EAS91_1_306UPAAXX 0 phiX 1180 255 34M2H * 0 0 TATTGACTCTACTGTAGACATTTTTACTTTTTAT * +HWI-EAS91_1_306UPAAXX 16 phiX 521 255 36M * 0 0 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGA * +HWI-EAS91_1_306UPAAXX 16 phiX 333 255 36M * 0 0 AGAGCGTGGATTACTATCTGAGTCCGATGCTGTTCA * +HWI-EAS91_1_306UPAAXX 0 phiX 2756 255 36M * 0 0 ATACCGTCAAGGACTGTGTGACTATTGACGTCCTTC * +HWI-EAS91_1_306UPAAXX 16 phiX 4957 255 36M * 0 0 ATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTATA * +HWI-EAS91_1_306UPAAXX 16 phiX 72 255 36M * 0 0 ATAAAGCAGGAATTACTACTGCTTGTTTACGAATTA * +HWI-EAS91_1_306UPAAXX 16 phiX 4249 255 36M * 0 0 TTACTGAGAAGTTAATGGATGAATTGGCACAATGCT * +HWI-EAS91_1_306UPAAXX 16 phiX 2592 255 36M * 0 0 CAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGA * +HWI-EAS91_1_306UPAAXX 16 phiX 1974 255 36M * 0 0 CGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTT * +HWI-EAS91_1_306UPAAXX 16 phiX 3540 255 36M * 0 0 AAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAA * +HWI-EAS91_1_306UPAAXX 16 phiX 4130 255 36M * 0 0 CATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTAT * diff -r 8d149264b777 -r efdb6fb5de34 test-data/lastz_wrapper_out3.tabular --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/lastz_wrapper_out3.tabular Fri Nov 27 10:53:50 2009 -0500 @@ -0,0 +1,26 @@ +phiX 1164 1165 + 5386 HWI-EAS91_1_306UPAAXX:6:1:112:1280 26 27 + 36 G T GTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTG GTTGGCGCTCTCCGTCTTTCTCCATTTCGTCGTG +phiX 2490 2491 + 5386 HWI-EAS91_1_306UPAAXX:6:1:486:822 10 11 - 36 C A ACCTGTTTTACAGACACCTAAAGCTACATCGTCAAC ACCTGTTTTAAAGACACCTAAAGCTACATCGTCAAC +phiX 2205 2206 + 5386 HWI-EAS91_1_306UPAAXX:6:1:217:737 14 15 + 36 G T GTTCAGTTGTTGCAGTGGAATA GTTCAGTTGTTGCATTGGAATA +phiX 2173 2174 + 5386 HWI-EAS91_1_306UPAAXX:6:1:259:1389 26 27 + 36 G T GCGTACTTATTCGCCACCATGATTATGACCAGTGTT GCGTACTTATTCGCCACCATGATTATTACCAGTGTT +phiX 578 579 + 5386 HWI-EAS91_1_306UPAAXX:6:1:317:1411 8 9 + 36 G T GTACGCTGGACTTTGTGGGATACCCTCGCTTTCCT GTACGCTGTACTTTGTGGGATACCCTCGCTTTCCT +phiX 2992 2993 + 5386 HWI-EAS91_1_306UPAAXX:6:1:458:1629 23 24 + 36 G T GCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGT GCTGGTGGCGCCATGTCTAAATTTTTTGGAGGCGGT +phiX 4783 4784 + 5386 HWI-EAS91_1_306UPAAXX:6:1:646:59 18 19 - 36 C T CATTTCCATGCGGTGCACTTTATGCGGACACTTCC CATTTCCATGCGGTGCATTTTATGCGGACACTTCC +phiX 1649 1650 + 5386 HWI-EAS91_1_306UPAAXX:6:1:295:694 24 25 + 36 A G GTGATTACTTCATGCAGCGTTACCATGATGTTATTT GTGATTACTTCATGCAGCGTTACCGTGATGTTATTT +phiX 1995 1996 + 5386 HWI-EAS91_1_306UPAAXX:6:1:590:331 14 15 + 36 G T GAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGAT GAAATTTCTATGAATGATGTTTTCCGTTCTGGTGAT +phiX 3261 3262 + 5386 HWI-EAS91_1_306UPAAXX:6:1:221:1238 17 18 - 36 C A AAGTCTGCCGCTGATAAAGGAAAGGATAC AAGTCTGCCGATGATAAAGGAAAGGATAC +phiX 4109 4110 + 5386 HWI-EAS91_1_306UPAAXX:6:1:478:252 12 13 - 36 C A ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC ATGCTTGGCTTCAATAAGCAGATGGATAACCGCATC +phiX 3126 3127 + 5386 HWI-EAS91_1_306UPAAXX:6:1:589:96 28 29 - 36 A G CAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCC CAAGGCTCTAATGTTCCTAACCCTGATGGGGCCGCC +phiX 2569 2570 + 5386 HWI-EAS91_1_306UPAAXX:6:1:1472:716 23 24 + 36 G T TGGTAATGGTGGTTTTCTTCATTGCATTCAG TGGTAATGGTGGTTTTCTTCATTTCATTCAG +phiX 2972 2973 + 5386 HWI-EAS91_1_306UPAAXX:6:1:836:1204 17 18 + 36 G T TTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAAT TTGCTTCTGCTCTTGCTTGTGGCGCCATGTCTAAAT +phiX 2234 2235 + 5386 HWI-EAS91_1_306UPAAXX:6:1:172:1412 13 14 - 36 C A AAATTTAATGTGACCGTTTATCGCAATCTGCCGACC AAATTTAATGTGAACGTTTATCGCAATCTGCCGACC +phiX 2902 2903 + 5386 HWI-EAS91_1_306UPAAXX:6:1:540:1415 18 19 + 36 G T TTATTAAAGAGATTATTTGTCTCCAGCCACTTA TTATTAAAGAGATTATTTTTCTCCAGCCACTTA +phiX 2837 2838 + 5386 HWI-EAS91_1_306UPAAXX:6:1:253:1162 14 15 - 36 T A ATGGTTTGGTCTAACTTTACCGCTACTAAATGC ATGGTTTGGTCAAACTTTACCGCTACTAAATGC +phiX 4662 4663 + 5386 HWI-EAS91_1_306UPAAXX:6:1:828:754 26 27 + 36 G T TTTGCGTGACTATTTTCGTGATATTGGTCGTATGGT TTTGCGTGACTATTTTCGTGATATTGTTCGTATGGT +phiX 4202 4203 + 5386 HWI-EAS91_1_306UPAAXX:6:1:891:1437 19 20 + 36 G T TAATGGTGATATGTATGTTGACGGCCATAAGGCTG TAATGGTGATATGTATGTTTACGTCCATAAGGCTG +phiX 4206 4207 + 5386 HWI-EAS91_1_306UPAAXX:6:1:891:1437 23 24 + 36 G T TAATGGTGATATGTATGTTGACGGCCATAAGGCTG TAATGGTGATATGTATGTTTACGTCCATAAGGCTG +phiX 430 431 + 5386 HWI-EAS91_1_306UPAAXX:6:1:203:667 9 10 - 36 T C AGACCGCTTTGGCCTCTATTAAGC AGACCGCCTTGGCCTCTATTAAGC +phiX 4783 4784 + 5386 HWI-EAS91_1_306UPAAXX:6:1:1566:499 9 10 + 36 C T TGCGGTGCACTTTATGCGGACACTTCCTACAGGTAG TGCGGTGCATTTTATGCGGACACTTCCTACAGGTAG +phiX 2857 2858 + 5386 HWI-EAS91_1_306UPAAXX:6:1:578:971 15 16 + 36 G T CGCTACTAAATGCCGCGGATTGGTTTCG CGCTACTAAATTCCGCGGATTGGTTTCG +phiX 3112 3113 + 5386 HWI-EAS91_1_306UPAAXX:6:1:1721:1800 27 28 - 36 T C TCAAGGCTCTAATGTTCCTAACCC TCAAGGCTCTAATGTCCCTAACCC +phiX 4825 4826 + 5386 HWI-EAS91_1_306UPAAXX:6:1:815:1640 9 10 - 36 T G CCCTAATTTTGGTCGTCGGGTACGCAATCGCCGCCA CCCTAATTTGGGTCGTCGGGTACGCAATCGCCGCCA +phiX 4517 4518 + 5386 HWI-EAS91_1_306UPAAXX:6:1:1485:741 19 20 - 36 G A GAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGAT GAAATCGCGTAGAGGCTTTACTATTCAGCGTTTGAT diff -r 8d149264b777 -r efdb6fb5de34 test-data/phiX.fa --- a/test-data/phiX.fa Wed Nov 25 14:06:28 2009 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,79 +0,0 @@ ->phiX -GAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTTTCGGATATTTCTGATGAGTCGAAAAATTATCTT -GATAAAGCAGGAATTACTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAA -ATTCGACCTATCCTTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACTAACGATTCTG -TCAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTA -GATATGAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTGGATTACTATC -TGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGTTACTGAACAATCCGTACGTT -TCCAGACCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGATGATTT -CGATTTTCTGACGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCT -TGCGTTTATGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCG -TCATTGCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTAC -GGAAAACATTATTAATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTA -CGCGCAGGAAACACTGACGTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAATTACGTGCGGAAGGAG -TGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACT -AAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGC -CCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCA -TCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATTTCAACTACTCCGGTTATCGCTGGCGAC -TCCTTCGAGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTA -CTGTAGACATTTTTACTTTTTATGTCCCTCATCGTCACGTTTATGGTGAACAGTGGATTAAGTTCATGAA -GGATGGTGTTAATGCCACTCCTCTCCCGACTGTTAACACTACTGGTTATATTGACCATGCCGCTTTTCTT -GGCACGATTAACCCTGATACCAATAAAATCCCTAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACA -ACTATTTTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGATGATGC -TCGTTATGGTTTCCGTTGCTGCCATCTCAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTT -TCTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGC -ATACTGACCAAGAACGTGATTACTTCATGCAGCGTTACCATGATGTTATTTCTTCATTTGGAGGTAAAAC -CTCTTATGACGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCTATGATGTT -GATGGAACTGACCAAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAACATTCTGTGC -CGCGTTTCTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGCGAC -TAAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTTGACTTATACCGATATTGCTGGCGACCCTGTTTTG -TATGGCAACTTGCCGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGT -TTAAGATTGCTGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTGA -AGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGAT -TATGACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGAATAGTCAGGTTAAATTTAATGTGACCGTTT -ATCGCAATCTGCCGACCACTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATAAC -GCCGAAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGCGAAGCGCGGTAGGTTTTCTGC -TTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGT -TCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGACACCTAAAGCTACATCGTCAACGTTA -TATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTGGTTTTCTTCATTGCATTCAGATGGATACATCTG -TCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGC -CTGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTCCCGACTGCCTATGATGTTTATCCTTTG -AATGGTCGCCATGATGGTGGTTATTATACCGTCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGC -CGGGCAATAACGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTACTAAATGCCGCGGATTGGT -TTCGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGATTTATGTTTGGTG -CTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAA -AGCCGCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACCGATAACAATACTGTAGGCATGGGTGATGCT -GGTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCCCTAGTTTTGTTTCTG -GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGA -TAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTAT -CTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGG -TTGACGCCGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA -GATGCAAAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACTTCACGCCAGAATACGAAAGAC -CAGGTATATGCACAAAATGAGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGCGTTGCGTCTATTA -TGGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAAGCTCA -AACGGCTGGTCAGTATTTTACCAATGACCAAATCAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGAC -TTAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCTCTTCTCATATTGGCGCTACTGCAAAGGATATTT -CTAATGTCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTTTTCATGGTATTGATAAAGCTGTTGCCGA -TACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTCTAATTTGTCTAGGAAATAACCG -TCAGGATTGACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGGCTTTTTTATGGTTCGTT -CTTATTACCCTTCTGAATGTCACGCTGATTATTTTGACTTTGAGCGTATCGAGGCTCTTAAACCTGCTAT -TGAGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAATGCTTGGCTTCCATAAGCAGATGGATAACCGC -ATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATATGTATG -TTGACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTACTGAGAAGTTAATGGATGA -ATTGGCACAATGCTACAATGTGCTCCCCCAACTTGATATTAATAACACTATAGACCACCGCCCCGAAGGG -GACGAAAAATGGTTTTTAGAGAACGAGAAGACGGTTACGCAGTTTTGCCGCAAGCTGGCTGCTGAACGCC -CTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATT -GCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGATGAATGCAATGCGACAG -GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGATTAGAGGCGTTTT -ATGATAATCCCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTCTTGCTGCCGAGGGTCG -CAAGGCTAATGATTCACACGCCGACTGCTATCAGTATTTTTGTGTGCCTGAGTATGGTACAGCTAATGGC -CGTCTTCATTTCCATGCGGTGCACTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTC -GTCGGGTACGCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT -CGCAGTTCGCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAG -CCGCTTAAAGCTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTAAATACGTTAACAAAAAGTCAGATA -TGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACT -TCCCAAGAAGCTGTTCAGAATCAGAATGAGCCGCAACTTCGGGATGAAAATGCTCACAATGACAAATCTG -TCCACGGAGTGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAGC -AGAACGCAAAAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTTTGGCGGCGCAACC -TGTGACGACAAATCTGCTCAAATTTATGCGCGCTTCGATAAAAATGATTGGCGTATCCAACCTGCA - diff -r 8d149264b777 -r efdb6fb5de34 test-data/phiX.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/phiX.fasta Fri Nov 27 10:53:50 2009 -0500 @@ -0,0 +1,79 @@ +>phiX +GAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTTTCGGATATTTCTGATGAGTCGAAAAATTATCTT +GATAAAGCAGGAATTACTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAA +ATTCGACCTATCCTTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACTAACGATTCTG +TCAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTA +GATATGAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTGGATTACTATC +TGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGTTACTGAACAATCCGTACGTT +TCCAGACCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGATGATTT +CGATTTTCTGACGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCT +TGCGTTTATGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCG +TCATTGCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTAC +GGAAAACATTATTAATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTA +CGCGCAGGAAACACTGACGTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAATTACGTGCGGAAGGAG +TGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACT +AAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGC +CCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCA +TCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATTTCAACTACTCCGGTTATCGCTGGCGAC +TCCTTCGAGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTA +CTGTAGACATTTTTACTTTTTATGTCCCTCATCGTCACGTTTATGGTGAACAGTGGATTAAGTTCATGAA +GGATGGTGTTAATGCCACTCCTCTCCCGACTGTTAACACTACTGGTTATATTGACCATGCCGCTTTTCTT +GGCACGATTAACCCTGATACCAATAAAATCCCTAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACA +ACTATTTTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGATGATGC +TCGTTATGGTTTCCGTTGCTGCCATCTCAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTT +TCTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGC +ATACTGACCAAGAACGTGATTACTTCATGCAGCGTTACCATGATGTTATTTCTTCATTTGGAGGTAAAAC +CTCTTATGACGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCTATGATGTT +GATGGAACTGACCAAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAACATTCTGTGC +CGCGTTTCTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGCGAC +TAAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTTGACTTATACCGATATTGCTGGCGACCCTGTTTTG +TATGGCAACTTGCCGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGT +TTAAGATTGCTGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTGA +AGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGAT +TATGACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGAATAGTCAGGTTAAATTTAATGTGACCGTTT +ATCGCAATCTGCCGACCACTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATAAC +GCCGAAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGCGAAGCGCGGTAGGTTTTCTGC +TTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGT +TCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGACACCTAAAGCTACATCGTCAACGTTA +TATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTGGTTTTCTTCATTGCATTCAGATGGATACATCTG +TCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGC +CTGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTCCCGACTGCCTATGATGTTTATCCTTTG +AATGGTCGCCATGATGGTGGTTATTATACCGTCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGC +CGGGCAATAACGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTACTAAATGCCGCGGATTGGT +TTCGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGATTTATGTTTGGTG +CTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAA +AGCCGCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACCGATAACAATACTGTAGGCATGGGTGATGCT +GGTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCCCTAGTTTTGTTTCTG +GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGA +TAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTAT +CTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGG +TTGACGCCGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA +GATGCAAAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACTTCACGCCAGAATACGAAAGAC +CAGGTATATGCACAAAATGAGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGCGTTGCGTCTATTA +TGGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAAGCTCA +AACGGCTGGTCAGTATTTTACCAATGACCAAATCAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGAC +TTAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCTCTTCTCATATTGGCGCTACTGCAAAGGATATTT +CTAATGTCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTTTTCATGGTATTGATAAAGCTGTTGCCGA +TACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTCTAATTTGTCTAGGAAATAACCG +TCAGGATTGACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGGCTTTTTTATGGTTCGTT +CTTATTACCCTTCTGAATGTCACGCTGATTATTTTGACTTTGAGCGTATCGAGGCTCTTAAACCTGCTAT +TGAGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAATGCTTGGCTTCCATAAGCAGATGGATAACCGC +ATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATATGTATG +TTGACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTACTGAGAAGTTAATGGATGA +ATTGGCACAATGCTACAATGTGCTCCCCCAACTTGATATTAATAACACTATAGACCACCGCCCCGAAGGG +GACGAAAAATGGTTTTTAGAGAACGAGAAGACGGTTACGCAGTTTTGCCGCAAGCTGGCTGCTGAACGCC +CTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATT +GCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGATGAATGCAATGCGACAG +GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGATTAGAGGCGTTTT +ATGATAATCCCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTCTTGCTGCCGAGGGTCG +CAAGGCTAATGATTCACACGCCGACTGCTATCAGTATTTTTGTGTGCCTGAGTATGGTACAGCTAATGGC +CGTCTTCATTTCCATGCGGTGCACTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTC +GTCGGGTACGCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT +CGCAGTTCGCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAG +CCGCTTAAAGCTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTAAATACGTTAACAAAAAGTCAGATA +TGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACT +TCCCAAGAAGCTGTTCAGAATCAGAATGAGCCGCAACTTCGGGATGAAAATGCTCACAATGACAAATCTG +TCCACGGAGTGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAGC +AGAACGCAAAAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTTTGGCGGCGCAACC +TGTGACGACAAATCTGCTCAAATTTATGCGCGCTTCGATAAAAATGATTGGCGTATCCAACCTGCA + diff -r 8d149264b777 -r efdb6fb5de34 tool-data/lastz_seqs.loc --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool-data/lastz_seqs.loc Fri Nov 27 10:53:50 2009 -0500 @@ -0,0 +1,2 @@ +#ref path +hg19 /depot/data2/galaxy/hg19/seq/hg19.2bit diff -r 8d149264b777 -r efdb6fb5de34 tool_conf.xml.sample --- a/tool_conf.xml.sample Wed Nov 25 14:06:28 2009 -0500 +++ b/tool_conf.xml.sample Fri Nov 27 10:53:50 2009 -0500 @@ -187,7 +187,7 @@ <tool file="solid_tools/solid_qual_boxplot.xml" /> </section> <section name="NGS: Mapping" id="solexa_tools"> -  + <tool file="sr_mapping/lastz_wrapper.xml" /> <tool file="sr_mapping/bowtie_wrapper.xml" /> <tool file="sr_mapping/bwa_wrapper.xml" /> <tool file="metag_tools/megablast_wrapper.xml" /> diff -r 8d149264b777 -r efdb6fb5de34 tools/sr_mapping/bwa_wrapper.xml --- a/tools/sr_mapping/bwa_wrapper.xml Wed Nov 25 14:06:28 2009 -0500 +++ b/tools/sr_mapping/bwa_wrapper.xml Fri Nov 27 10:53:50 2009 -0500 @@ -169,7 +169,7 @@ <test> <param name="solidSolexa" value="solid" /> <param name="refGenomeSource" value="history" /> - <param name="ownFile" value="phiX.fa" /> + <param name="ownFile" value="phiX.fasta" /> <param name="sPaired" value="single" /> <param name="input1" value="bwa_wrapper_in0.fastq" ftype="fastqsanger" /> <param name="source_select" value="pre_set" /> diff -r 8d149264b777 -r efdb6fb5de34 tools/sr_mapping/lastz_wrapper.py --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/sr_mapping/lastz_wrapper.py Fri Nov 27 10:53:50 2009 -0500 @@ -0,0 +1,133 @@ +#! /usr/bin/python + +""" + --lastzSeqsFileDir: Directory of local lastz_seqs.loc file +""" +import optparse, os, subprocess, shutil, sys, tempfile, threading +from Queue import Queue + +from galaxy import eggs +import pkg_resources +pkg_resources.require( 'bx-python' ) +from bx.seq.twobit import * + +def stop_err( msg ): + sys.stderr.write( "%s\n" % msg ) + sys.exit() + +class LastzJobRunner( object ): + """ + Lastz job runner backed by a pool of "num_threads" worker threads. FIFO scheduling + """ + def __init__( self, num_threads, commands ): + """Start the job runner with "num_threads" worker threads""" + # start workers + self.queue = Queue() + for command in commands: + self.queue.put( command ) + self.threads = [] + for i in range( num_threads ): + worker = threading.Thread( target=self.run_next ) + worker.start() + self.threads.append( worker ) + def run_next( self ): + """Run the next command, waiting until one is available if necessary""" + while not self.queue.empty(): + command = self.queue.get() + self.run_job( command ) + def run_job( self, command ): + try: + proc = subprocess.Popen( args=command, shell=True ) + sts = os.waitpid( proc.pid, 0 ) + except Exception, e: + stop_err( "Error executing command (%s) - %s" % ( str( command ), str( e ) ) ) + +def __main__(): + #Parse Command Line + parser = optparse.OptionParser() + parser.add_option( '', '--ref_name', dest='ref_name', help='' ) + parser.add_option( '', '--ref_source', dest='ref_source', help='' ) + parser.add_option( '', '--ref_sequences', dest='ref_sequences', help='Number of sequences in the reference dataset' ) + parser.add_option( '', '--source_select', dest='source_select', help='' ) + parser.add_option( '', '--out_format', dest='out_format', help='' ) + parser.add_option( '', '--input1', dest='input1', help='' ) + parser.add_option( '', '--input2', dest='input2', help='' ) + parser.add_option( '', '--pre_set_options', dest='pre_set_options', help='' ) + parser.add_option( '', '--strand', dest='strand', help='' ) + parser.add_option( '', '--seed', dest='seed', help='' ) + parser.add_option( '', '--transition', dest='transition', help='' ) + parser.add_option( '', '--gfextend', dest='gfextend', help='' ) + parser.add_option( '', '--nogfextend', dest='nogfextend', help='' ) + parser.add_option( '', '--chain', dest='chain', help='' ) + parser.add_option( '', '--nochain', dest='nochain', help='' ) + parser.add_option( '', '--gapped', dest='gapped', help='' ) + parser.add_option( '', '--nogapped', dest='nogapped', help='' ) + parser.add_option( '', '--O', dest='O', help='' ) + parser.add_option( '', '--E', dest='E', help='' ) + parser.add_option( '', '--X', dest='X', help='' ) + parser.add_option( '', '--Y', dest='Y', help='' ) + parser.add_option( '', '--K', dest='K', help='' ) + parser.add_option( '', '--L', dest='L', help='' ) + parser.add_option( '', '--entropy', dest='entropy', help='' ) + parser.add_option( '', '--identity_min', dest='identity_min', help='' ) + parser.add_option( '', '--identity_max', dest='identity_max', help='' ) + parser.add_option( '', '--coverage', dest='coverage', help='' ) + parser.add_option( '', '--format', dest='format', help='' ) + parser.add_option( '', '--output', dest='output', help='The output file' ) + parser.add_option( '', '--num_threads', dest='num_threads', help='' ) + parser.add_option( '', '--lastzSeqsFileDir', dest='lastzSeqsFileDir', help='Directory of local lastz_seqs.loc file' ) + ( options, args ) = parser.parse_args() + + commands = [] + if options.ref_name != 'None': + ref_name = '%s::' % options.ref_name + else: + ref_name = '' + # Prepare for commonly-used preset options + if options.source_select == 'pre_set': + set_options = '--%s' % options.pre_set_options + # Prepare for user-specified options + else: + set_options = '%s %s --%s --%s --%s --%s O=%s E=%s X=%s Y=%s K=%s L=%s %s' % \ + ( options.gfextend, options.chain, options.gapped, options.strand, + options.seed, options.transition, options.O, options.E, options.X, + options.Y, options.K, options.L, options.entropy ) + # Specify input2 and add [fullnames] modifier if output format is diffs + if options.format == 'diffs': + input2 = '%s[fullnames]' % options.input2 + else: + input2 = options.input2 + if options.format == 'tabular': + # Change output format to general if it's tabular and add field names for tabular output + format = 'general' + tabular_fields = ':score,name1,strand1,size1,start1,zstart1,end1,length1,text1,name2,strand2,size2,start2,zstart2,end2,start2+,zstart2+,end2+,length2,text2,diff,cigar,identity,coverage,gaprate,diagonal,shingle' + else: + format = options.format + tabular_fields = '' + if options.ref_source == 'history': + # Reference is a fasta dataset from the history, so split job across number of + # sequences in the dataset + try: + error_msg = "The reference dataset is missing metadata, click the pencil icon in the history item and 'auto-detect' the metadata attributes." + ref_sequences = int( options.ref_sequences ) + if ref_sequences < 1: + stop_err( error_msg ) + except: + stop_err( error_msg ) + for seq in range( ref_sequences ): + command = 'lastz %s%s %s %s --ambiguousn --nolaj --identity=%s..%s --coverage=%s --format=%s%s >> %s' % \ + ( ref_name, options.input1, input2, set_options, options.identity_min, + options.identity_max, options.coverage, format, tabular_fields, options.output ) + commands.append( command ) + else: + # Reference is a locally cached 2bit file, split job across number of chroms in 2bit file + tbf = TwoBitFile( open( options.input1, 'r' ) ) + for chrom in tbf.keys(): + command = 'lastz %s%s/%s %s %s --ambiguousn --nolaj --identity=%s..%s --coverage=%s --format=%s%s >> %s' % \ + ( ref_name, options.input1, chrom, input2, set_options, options.identity_min, + options.identity_max, options.coverage, format, tabular_fields, options.output ) + commands.append( command ) + tbf.close() + job_runner = LastzJobRunner( int( options.num_threads ), commands ) + +if __name__=="__main__": __main__() diff -r 8d149264b777 -r efdb6fb5de34 tools/sr_mapping/lastz_wrapper.xml --- a/tools/sr_mapping/lastz_wrapper.xml Wed Nov 25 14:06:28 2009 -0500 +++ b/tools/sr_mapping/lastz_wrapper.xml Fri Nov 27 10:53:50 2009 -0500 @@ -1,124 +1,183 @@ -<tool id="lastz_wrapper_1" name="Lastz" version="1.0.0"> - <description> map short reads against reference sequence</description> - <command> - #if ($params.source_select=="pre_set" and $seq_name.how_to_name=="No" and $out_format.value=="diffs") #lastz $input1 ${input2}[fullnames] --${params.pre_set_options} --ambiguousn --nolaj --identity=${min_ident}..${max_ident} --census32=$output2 --coverage=$min_cvrg --format=$out_format > $output1 - #elif ($params.source_select=="pre_set" and $seq_name.how_to_name=="Yes" and $out_format.value=="diffs") #lastz $seq_name.ref_name::$input1 ${input2}[fullnames] --${params.pre_set_options} --ambiguousn --nolaj --identity=${min_ident}..${max_ident} --census32=$output2 --coverage=$min_cvrg --format=$out_format > $output1 - #elif ($params.source_select=="full" and $seq_name.how_to_name=="No" and $out_format.value=="diffs") #lastz $input1 ${input2}[fullnames] $params.strand $params.seed $params.transition O=$params.O E=$params.E X=$params.X Y=$params.Y K=$params.K L=$params.L $params.entropy --ambiguousn --nolaj --identity=${min_ident}..${max_ident} --census32=$output2 --coverage=$min_cvrg --format=$out_format > $output1 - #elif ($params.source_select=="full" and $seq_name.how_to_name=="Yes" and $out_format.value=="diffs") #lastz $seq_name.ref_name::$input1 ${input2}[fullnames] $params.strand $params.seed $params.transition O=$params.O E=$params.E X=$params.X Y=$params.Y K=$params.K L=$params.L $params.entropy --ambiguousn --nolaj --identity=${min_ident}..${max_ident} --census32=$output2 --coverage=$min_cvrg --format=$out_format > $output1 - #elif ($params.source_select=="pre_set" and $seq_name.how_to_name=="No" and $out_format.value=="maf") #lastz $input1 read::${input2} --${params.pre_set_options} --ambiguousn --identity=${min_ident}..${max_ident} --census32=$output2 --coverage=$min_cvrg --format=$out_format > $output1 - #elif ($params.source_select=="pre_set" and $seq_name.how_to_name=="Yes" and $out_format.value=="maf") #lastz $seq_name.ref_name::$input1 read::${input2} --${params.pre_set_options} --ambiguousn --identity=${min_ident}..${max_ident} --census32=$output2 --coverage=$min_cvrg --format=$out_format > $output1 - #elif ($params.source_select=="full" and $seq_name.how_to_name=="No" and $out_format.value=="maf") #lastz $input1 read::${input2} $params.strand $params.seed $params.transition O=$params.O E=$params.E X=$params.X Y=$params.Y K=$params.K L=$params.L $params.entropy --ambiguousn --identity=${min_ident}..${max_ident} --census32=$output2 --coverage=$min_cvrg --format=$out_format > $output1 - #elif ($params.source_select=="full" and $seq_name.how_to_name=="Yes" and $out_format.value=="maf") #lastz $seq_name.ref_name::$input1 read::${input2} $params.strand $params.seed $params.transition O=$params.O E=$params.E X=$params.X Y=$params.Y K=$params.K L=$params.L $params.entropy --ambiguousn --identity=${min_ident}..${max_ident} --census32=$output2 --coverage=$min_cvrg --format=$out_format > $output1 - #end if# - </command> - <inputs> - <param name="input2" format="fasta" type="data" label="Align sequencing reads" /> - <param name="input1" format="fasta" type="data" label="against reference" help="must be a single sequence"/> - <param name="out_format" type="select" label="Select output format"> - <option value="diffs">Polymorphisms</option> - <option value="maf-">Alignments in MAF format</option> - </param> - - <conditional name="params"> - <param name="source_select" type="select" label="Lastz settings to use" help="For most mapping needs use Commonly used settings. If you want full control use Full List"> - <option value="pre_set">Commonly used</option> - <option value="full">Full Parameter List</option> - </param> - <when value="pre_set"> - <param name="pre_set_options" type="select" label="Select mapping mode"> - <option value="yasra95short">Illumina-Solexa/AB-SOLiD 95% identity</option> - <option value="yasra85short">Illumina-Solexa/AB-SOLiD 85% identity</option> - <option value="yasra98">Roche-454 98% identity</option> - <option value="yasra95">Roche-454 95% identity</option> - <option value="yasra90">Roche-454 90% identity</option> - <option value="yasra85">Roche-454 85% identity</option> - <option value="yasra75">Roche-454 75% identity</option> - </param> - </when> - <when value="full"> - <param name="strand" type="select" label="Which strand to search?"> - <option value="--strand=both">Both</option> - <option value="--strand=plus">Search forward strand only (the one in the reference)</option> - <option value="--strand=minus">Search the reverse complement strand only (opposite of the reference)</option> - </param> - <param name="seed" type="select" label="Select seeding settings" help="allows you set word size and number of mismatches"> - <option value="--seed=12of19">Seed hits require a 19 bp word with matches in 12 specific positions</option> - <option value="--seed=14of22">Seed hits require a 22 bp word with matches in 14 specific positions</option> - </param> - <param name="transition" type="select" label="Select transition settings" help="affects the number of allowed transition substitutions"> - <option value="--transition">Allow one transition in each seed hit</option> - <option value="--transition2">Allow two transitions in a seed hit </option> - <option value="--notransition">Don't allow any transitions in seed hits</option> - </param> - <param name="gfextend" type="boolean" truevalue="--gfextend" falsevalue="--nogfextend " label="Perform gap-free extension of seed hits to HSPs (high scoring segment pairs)?" /> - <param name="chain" type="boolean" truevalue="--chain" falsevalue="--nochain" label="Perform chaining of HSPs?" /> - <param name="gapped" type="boolean" truevalue="--gapped" falsevalue="--nogapped" label="Perform gapped alignment (instead of gap-free)?" /> - <param name="O" type="integer" size="5" value="400" label="Gap opening penalty"/> - <param name="E" type="integer" size="5" value="30" label="Gap extension penalty"/> - <param name="X" type="integer" size="5" value="910" label="X-drop threshold "/> - <param name="Y" type="integer" size="5" value="9370" label="Y-drop threshold"/> - <param name="K" type="integer" size="5" value="3000" label="Set the threshold for HSPs (ungapped extensions scoring lower are discarded)"/> - <param name="L" type="integer" size="5" value="3000" label="Set the threshold for gapped alignments (gapped extensions scoring lower are discarded)"/> - <param name="entropy" type="boolean" truevalue="--entropy" falsevalue="--noentropy " label="Involve entropy when filtering HSPs?" /> - </when> - </conditional> - <conditional name="seq_name"> - <param name="how_to_name" type="select" label="Do you want to modify reference name?"> - <option value="No">No</option> - <option value="Yes">Yes</option> - </param> - <when value="Yes"> - <param name="ref_name" type="text" size="25" value="Type sequence name here" label="Enter name for the Reference sequence"/> - </when> - <when value="No" /> - </conditional> - <param name="min_ident" type="integer" size="3" value="0" label="Do not report matches below this identity (%)"/> - <param name="max_ident" type="integer" size="3" value="100" label="Do not report matches above this identity (%)"/> - <param name="min_cvrg" type="integer" size="3" value="0" label="Do not report matches that cover less than this fraction (%) of each read"/> - </inputs> +<tool id="lastz_wrapper_2" name="Lastz" version="1.0.0"> + <description> map short reads against reference sequence</description> + <command interpreter="python">lastz_wrapper.py +#if $seq_name.how_to_name=="Yes": +--ref_name=$seq_name.ref_name +#else: +--ref_name="None" +#end if +--ref_source=$source.ref_source --source_select=$params.source_select --out_format=$out_format.value --input1=$source.input1 --input2=$input2 +#if $source.ref_source=="history": +--ref_sequences=$input1.metadata.sequences +#else: +--ref_sequences="None" +#end if +#if $params.source_select=="pre_set": +--pre_set_options=${params.pre_set_options} --strand="None" --seed="None" --transition="None" --O="None" --E="None" --X="None" --Y="None" --K="None" --L="None" --entropy="None" +#else: +--pre_set_options="None" --strand=$params.strand --seed=$params.seed --transition=$params.transition --O=$params.O --E=$params.E --X=$params.X --Y=$params.Y --K=$params.K --L=$params.L --entropy=$params.entropy +#end if +--identity_min=$min_ident --identity_max=$max_ident --coverage=$min_cvrg --format=$out_format --output=$output1 --num_threads=$num_threads --lastzSeqsFileDir=${GALAXY_DATA_INDEX_DIR} + </command> + <inputs> + <param name="input2" format="fasta" type="data" label="Align sequencing reads in" /> + <conditional name="source"> + <param name="ref_source" type="select" label="Against reference sequences"> + <option value="cached">that are locally cached</option> + <option value="history">in your history</option> + </param> + <when value="cached"> + <param name="input1" type="select" label="Using reference genome" help="If your genome of interest is not listed, contact the Galaxy team"> + <options from_file="lastz_seqs.loc"> + <column name="value" index="1" /> + <column name="name" index="0" /> + </options> + </param> + </when> + <when value="history"> + <param name="input1" type="data" format="fasta" label="Select a reference dataset" help="must be a single sequence" /> + </when> + </conditional> + <param name="out_format" type="select" label="Output format"> + <option value="sam">SAM</option> + <option value="diffs">Polymorphisms</option> + <option value="tabular">Tabular</option> + </param> + <conditional name="params"> + <param name="source_select" type="select" label="Lastz settings to use" help="For most mapping needs use Commonly used settings. If you want full control use Full List"> + <option value="pre_set">Commonly used</option> + <option value="full">Full Parameter List</option>

1 0

[hg] galaxy 3124: Added usage information, added/updated relevan...
by Greg Von Kuster 02 Dec '09

02 Dec '09

details: http://www.bx.psu.edu/hg/galaxy/rev/341d7c1c5c9a changeset: 3124:341d7c1c5c9a user: Kelly Vincent <kpvincent(a)bx.psu.edu> date: Mon Nov 30 09:05:28 2009 -0500 description: Added usage information, added/updated relevant test files, and got all tests working for Lastz diffstat: test-data/lastz_wrapper_out1.sam | 2 - test-data/lastz_wrapper_out2.tabular | 453 +++++++++++++++++++++++++++++++++++++ tools/sr_mapping/lastz_wrapper.py | 106 +++++--- tools/sr_mapping/lastz_wrapper.xml | 39 +- 4 files changed, 539 insertions(+), 61 deletions(-) diffs (742 lines): diff -r efdb6fb5de34 -r 341d7c1c5c9a test-data/lastz_wrapper_out1.sam --- a/test-data/lastz_wrapper_out1.sam Fri Nov 27 10:53:50 2009 -0500 +++ b/test-data/lastz_wrapper_out1.sam Mon Nov 30 09:05:28 2009 -0500 @@ -1,5 +1,3 @@ -@HD VN:1.0 SO:unsorted -@SQ SN:phiX LN:5386 HWI-EAS91_1_306UPAAXX 16 phiX 4295 255 1H35M * 0 0 CCCCCAACTTGATATTAATAACACTATAGACCACC * HWI-EAS91_1_306UPAAXX 16 phiX 3522 255 36M * 0 0 ATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGC * HWI-EAS91_1_306UPAAXX 0 phiX 229 255 36M * 0 0 GATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTC * diff -r efdb6fb5de34 -r 341d7c1c5c9a test-data/lastz_wrapper_out2.tabular --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/lastz_wrapper_out2.tabular Mon Nov 30 09:05:28 2009 -0500 @@ -0,0 +1,453 @@ +#score name1 strand1 size1 start1 zstart1 end1 length1 text1 name2 strand2 size2 start2 zstart2 end2 start2+ zstart2+ end2+ length2 text2 diff cigar identity idPct coverage covPct gaprate gapPct diagonal shingle +3311 PHIX174 + 5386 4295 4294 4329 35 CCCCCAACTTGATATTAATAACACTATAGACCACC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CCCCCAACTTGATATTAATAACACTATAGACCACC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4293 NA +3429 PHIX174 + 5386 3522 3521 3557 36 ATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3521 NA +3429 PHIX174 + 5386 229 228 264 36 GATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 228 NA +3411 PHIX174 + 5386 2071 2070 2106 36 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2070 NA +3311 PHIX174 + 5386 1067 1066 1101 35 GGTCAGATTGGTCGTCTTATTACCATTTCAACTAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GGTCAGATTGGTCGTCTTATTACCATTTCAACTAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1065 NA +3429 PHIX174 + 5386 1788 1787 1823 36 GTCGTGTTCAACAGACCTATAAACATTCTGTGCCGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCGTGTTCAACAGACCTATAAACATTCTGTGCCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1787 NA +3393 PHIX174 + 5386 1193 1192 1228 36 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1192 NA +3206 PHIX174 + 5386 4563 4562 4598 36 GGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTTGGTTTATCGTTTTTGACACTCTCACGTTGTCT .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4562 NA +3447 PHIX174 + 5386 2668 2667 2703 36 GTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2667 NA +3402 PHIX174 + 5386 4248 4247 4283 36 GTTACTGAGAAGTTAATGGATGAATTGGCACAATGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTACTGAGAAGTTAATGGATGAATTGGCACAATGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4247 NA +3393 PHIX174 + 5386 2863 2862 2898 36 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2862 NA +3402 PHIX174 + 5386 2557 2556 2592 36 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2556 NA +3184 PHIX174 + 5386 2511 2510 2544 34 GTCAACGTTATATTTTGATAGTTTGACGGTTAAT HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 GTCAACGTTATATTTTGATAGTTTGACGGTTAAT .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 2510 NA +3420 PHIX174 + 5386 5152 5151 5187 36 GGATGAAAATGCTCACAATGACAAATCTGTCCACGG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGATGAAAATGCTCACAATGACAAATCTGTCCACGG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5151 NA +3402 PHIX174 + 5386 1590 1589 1625 36 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1589 NA +3456 PHIX174 + 5386 4884 4883 4919 36 GGTTACAGTATGCCCATCGCAGTTCGCTACACGCAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTTACAGTATGCCCATCGCAGTTCGCTACACGCAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4883 NA +3411 PHIX174 + 5386 5052 5051 5087 36 GCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5051 NA +3447 PHIX174 + 5386 4909 4908 4944 36 GCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4908 NA +3393 PHIX174 + 5386 2863 2862 2898 36 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2862 NA +3197 PHIX174 + 5386 4173 4172 4208 36 GTTGAGTTCGATAATGGTGATATGTATGTTGACGGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTGAGTTCGATAATGGTGATATGTATGTTTACGGC ..............................x..... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4172 NA +3429 PHIX174 + 5386 1701 1700 1736 36 GTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1700 NA +3429 PHIX174 + 5386 1683 1682 1718 36 CTTATGACGCTGACAACCGTCCTTTACTTGTCATGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTATGACGCTGACAACCGTCCTTTACTTGTCATGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1682 NA +3365 PHIX174 + 5386 3126 3125 3160 35 GAGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GAGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 3124 NA +3420 PHIX174 + 5386 1665 1664 1700 36 CATTTGGAGGTAAAACCTCTTATGACGCTGACAACC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CATTTGGAGGTAAAACCTCTTATGACGCTGACAACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1664 NA +3402 PHIX174 + 5386 3213 3212 3248 36 GTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3212 NA +3411 PHIX174 + 5386 3420 3419 3455 36 GAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3419 NA +3465 PHIX174 + 5386 513 512 548 36 GTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 512 NA +3179 PHIX174 + 5386 2879 2878 2914 36 ATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATAAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC ..x................................. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2878 NA +3438 PHIX174 + 5386 4471 4470 4506 36 GTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4470 NA +3393 PHIX174 + 5386 1229 1228 1264 36 GTTTATGGTGAACAGTGGATTAAGTTCATGAAGGAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTTATGGTGAACAGTGGATTAAGTTCATGAAGGAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1228 NA +3393 PHIX174 + 5386 615 614 650 36 GAGTTTATTGCTGCCGTCATTGCTTATTATGTTCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGTTTATTGCTGCCGTCATTGCTTATTATGTTCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 614 NA +3429 PHIX174 + 5386 257 256 292 36 GCACGTTCGTCAAGGACTGGTTTAGATATGAGTCAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCACGTTCGTCAAGGACTGGTTTAGATATGAGTCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 256 NA +3366 PHIX174 + 5386 2381 2380 2416 36 TTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2380 NA +3429 PHIX174 + 5386 4098 4097 4133 36 ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4097 NA +3438 PHIX174 + 5386 5099 5098 5134 36 GCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5098 NA +3393 PHIX174 + 5386 4254 4253 4289 36 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4253 NA +3411 PHIX174 + 5386 3408 3407 3443 36 GACAATCAGAAAGAGATTGCCGAGATGCAAAATGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACAATCAGAAAGAGATTGCCGAGATGCAAAATGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3407 NA +3429 PHIX174 + 5386 4043 4042 4078 36 GGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4042 NA +3411 PHIX174 + 5386 4560 4559 4595 36 GATGGTTGGTTTATCGTTTTTGACACTCTCACGTTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATGGTTGGTTTATCGTTTTTGACACTCTCACGTTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4559 NA +3411 PHIX174 + 5386 1888 1887 1923 36 GACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1887 NA +3311 PHIX174 + 5386 3434 3433 3468 35 GCAAAATGAGACTCAAAAAGAGATTGCTGGCATTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GCAAAATGAGACTCAAAAAGAGATTGCTGGCATTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 3432 NA +3438 PHIX174 + 5386 4539 4538 4574 36 GCAATGCGACAGGCTCATGCTGATGGTTGGTTTATC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAATGCGACAGGCTCATGCTGATGGTTGGTTTATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4538 NA +3420 PHIX174 + 5386 1828 1827 1863 36 CTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1827 NA +3420 PHIX174 + 5386 3708 3707 3743 36 GACTTAGTTCATCAGCAAACGCAGAATCAGCGGTAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACTTAGTTCATCAGCAAACGCAGAATCAGCGGTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3707 NA +3465 PHIX174 + 5386 3326 3325 3361 36 GCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3325 NA +3411 PHIX174 + 5386 4176 4175 4211 36 GAGTTCGATAATGGTGATATGTATGTTGACGGCCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGTTCGATAATGGTGATATGTATGTTGACGGCCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4175 NA +3402 PHIX174 + 5386 22 21 57 36 GCAGAAGTTAACACTTTCGGATATTTCTGATGAGTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAGAAGTTAACACTTTCGGATATTTCTGATGAGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 21 NA +3402 PHIX174 + 5386 2557 2556 2592 36 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2556 NA +3420 PHIX174 + 5386 3277 3276 3312 36 ATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3276 NA +3438 PHIX174 + 5386 3592 3591 3627 36 AGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3591 NA +3302 PHIX174 + 5386 358 357 392 35 GATGCTGTTCAACCACTAATAGGTAAGAAATCATG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GATGCTGTTCAACCACTAATAGGTAAGAAATCATG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 357 NA +3420 PHIX174 + 5386 1775 1774 1810 36 GGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1774 NA +3393 PHIX174 + 5386 3504 3503 3539 36 GTATATGCACAAAATGAGATGCTTGCTTATCAACAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTATATGCACAAAATGAGATGCTTGCTTATCAACAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3503 NA +3420 PHIX174 + 5386 1554 1553 1589 36 CGACTTCTACCACATCTATTGACATTATGGGTCTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGACTTCTACCACATCTATTGACATTATGGGTCTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1553 NA +3447 PHIX174 + 5386 1002 1001 1037 36 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1001 NA +3420 PHIX174 + 5386 3626 3625 3661 36 GCTTACTCAAGCTCAAACGGCTGGTCAGTATTTTAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCTTACTCAAGCTCAAACGGCTGGTCAGTATTTTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3625 NA +3420 PHIX174 + 5386 3572 3571 3607 36 GGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3571 NA +3438 PHIX174 + 5386 1265 1264 1300 36 GGTGTTAATGCCACTCCTCTCCCGACTGTTAACACT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTGTTAATGCCACTCCTCTCCCGACTGTTAACACT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1264 NA +3411 PHIX174 + 5386 1332 1331 1367 36 GCACGATTAACCCTGATACCAATAAAATCCCTAAGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCACGATTAACCCTGATACCAATAAAATCCCTAAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1331 NA +3393 PHIX174 + 5386 4254 4253 4289 36 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4253 NA +3411 PHIX174 + 5386 4170 4169 4205 36 GGCGTTGAGTTCGATAATGGTGATATGTATGTTGAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGCGTTGAGTTCGATAATGGTGATATGTATGTTGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4169 NA +3465 PHIX174 + 5386 3119 3118 3154 36 CCCTGATGAGGCCGCCCCTAGTTTTGTTTCTGGTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CCCTGATGAGGCCGCCCCTAGTTTTGTTTCTGGTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3118 NA +3438 PHIX174 + 5386 1670 1669 1705 36 GGAGGTAAAACCTCTTATGACGCTGACAACCGTCCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGAGGTAAAACCTCTTATGACGCTGACAACCGTCCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1669 NA +3420 PHIX174 + 5386 640 639 675 36 ATTATGTTCATCCCGTCAACATTCAAACGGCCTGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTATGTTCATCCCGTCAACATTCAAACGGCCTGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 639 NA +3311 PHIX174 + 5386 2750 2749 2784 35 GTTATTATACCGTCAAGGACTGTGTGACTATTGAC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GTTATTATACCGTCAAGGACTGTGTGACTATTGAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2749 NA +3402 PHIX174 + 5386 4606 4605 4641 36 GATTAGAGGCGTTTTATGATAATCCCAATGCTTTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GATTAGAGGCGTTTTATGATAATCCCAATGCTTTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4605 NA +3420 PHIX174 + 5386 3573 3572 3608 36 GAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3572 NA +3420 PHIX174 + 5386 5105 5104 5140 36 GCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5104 NA +3420 PHIX174 + 5386 1674 1673 1709 36 GTAAAACCTCTTATGACGCTGACAACCGTCCTTTAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTAAAACCTCTTATGACGCTGACAACCGTCCTTTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1673 NA +3438 PHIX174 + 5386 4095 4094 4130 36 CCAATGCTTGGCTTCCATAAGCAGATGGATAACCGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CCAATGCTTGGCTTCCATAAGCAGATGGATAACCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4094 NA +3411 PHIX174 + 5386 4551 4550 4586 36 GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4550 NA +3411 PHIX174 + 5386 3284 3283 3319 36 TGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3283 NA +3402 PHIX174 + 5386 4005 4004 4040 36 GAATGTCACGCTGATTATTTTGACTTTGAGCGTATC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAATGTCACGCTGATTATTTTGACTTTGAGCGTATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4004 NA +3420 PHIX174 + 5386 2479 2478 2514 36 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2478 NA +3420 PHIX174 + 5386 1082 1081 1117 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1081 NA +3338 PHIX174 + 5386 3045 3044 3079 35 GCTACCGATAACAATACTGTAGGCATGGGTGATGC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GCTACCGATAACAATACTGTAGGCATGGGTGATGC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 3043 NA +3492 PHIX174 + 5386 1115 1114 1150 36 GGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1114 NA +3206 PHIX174 + 5386 636 635 671 36 GCTTATTATGTTCATCCCGTCAACATTCAAACGGCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTTATTATGTTCATCCCGTCAACATTCAAACGTCC .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 635 NA +3060 PHIX174 + 5386 1139 1138 1172 34 GTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTG HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 GTTGGCGCTCTCCGTCTTTCTCCATTTCGTCGTG ..........................x....... 34M 33/34 97.1% 34/36 94.4% 0/34 0.0% 1138 NA +3447 PHIX174 + 5386 852 851 887 36 CTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 851 NA +3429 PHIX174 + 5386 5272 5271 5307 36 GATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5271 NA +3127 PHIX174 + 5386 4330 4329 4365 36 GCCCCGAAGGGGACGAAAAATGGTTTTTAGAGAACG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCCCGAAGGGGACNANAAATGGTTTTTAGAGAACG ..............X.X................... 36M 34/34 100.0% 36/36 100.0% 0/36 0.0% 4329 NA +3465 PHIX174 + 5386 4891 4890 4926 36 GTATGCCCATCGCAGTTCGCTACACGCAGGACGCTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTATGCCCATCGCAGTTCGCTACACGCAGGACGCTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4890 NA +3429 PHIX174 + 5386 4783 4782 4818 36 AtTTTATGCGGACACTTCCTACAGGTAGCGTTGACC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTTTATGCGGACACTTCCTACAGGTAGCGTTGACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4782 NA +3438 PHIX174 + 5386 3619 3618 3654 36 GCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3618 NA +3215 PHIX174 + 5386 4455 4454 4490 36 GGTATTAAGGATGAGTGTTCAAGATTGCTGGAGGCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTATTAAGGATGAGTGTTCAAGATTGCTGGATGCC ................................x... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4454 NA +3393 PHIX174 + 5386 632 631 667 36 CATTGCTTATTATGTTCATCCCGTCAACATTCAAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CATTGCTTATTATGTTCATCCCGTCAACATTCAAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 631 NA +3347 PHIX174 + 5386 2964 2963 2998 35 GCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2963 NA +3447 PHIX174 + 5386 2763 2762 2798 36 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2762 NA +3465 PHIX174 + 5386 2766 2765 2801 36 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2765 NA +3429 PHIX174 + 5386 1719 1718 1754 36 GCTCTAATCTCTGGGCATCTGGCTATGATGTTGATG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTCTAATCTCTGGGCATCTGGCTATGATGTTGATG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1718 NA +3456 PHIX174 + 5386 3100 3099 3135 36 AAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3099 NA +3133 PHIX174 + 5386 598 597 632 35 GCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCGTC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GCTTTCCTGCTCCTGTTGAGTTTATTGCTTCCGTC .............................x..... 35M 34/35 97.1% 35/36 97.2% 0/35 0.0% 597 NA +3420 PHIX174 + 5386 4126 4125 4161 36 ACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4125 NA +3420 PHIX174 + 5386 4026 4025 4061 36 GACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4025 NA +3402 PHIX174 + 5386 2621 2620 2656 36 GTGCTGATATTGCTTTTGATGCCGACCCTAAATTTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTGCTGATATTGCTTTTGATGCCGACCCTAAATTTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2620 NA +3411 PHIX174 + 5386 2749 2748 2784 36 GGTTATTATACCGTCAAGGACTGTGTGACTATTGAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTTATTATACCGTCAAGGACTGTGTGACTATTGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2748 NA +3402 PHIX174 + 5386 379 378 414 36 GGTAAGAAATCATGAGTCAAGTTACTGAACAATCCG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTAAGAAATCATGAGTCAAGTTACTGAACAATCCG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 378 NA +3438 PHIX174 + 5386 2160 2159 2195 36 GCCACCATGATTATGACCAGTGTTTCCAGTCCGTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCACCATGATTATGACCAGTGTTTCCAGTCCGTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2159 NA +3393 PHIX174 + 5386 2873 2872 2908 36 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2872 NA +3365 PHIX174 + 5386 1508 1507 1542 35 TGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 TGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1506 NA +3420 PHIX174 + 5386 3863 3862 3898 36 TTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3862 NA +3429 PHIX174 + 5386 5187 5186 5222 36 GAGTGCTTAATCCAACTTACCAAGCTGGGTTACGAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGTGCTTAATCCAACTTACCAAGCTGGGTTACGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5186 NA +3447 PHIX174 + 5386 4196 4195 4231 36 GTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4195 NA +3411 PHIX174 + 5386 635 634 670 36 TGCTTATTATGTTCATCCCGTCAACATTCAAACGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGCTTATTATGTTCATCCCGTCAACATTCAAACGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 634 NA +3311 PHIX174 + 5386 1294 1293 1328 35 TAACACTACTGGTTATATTGACCATGCCGCTTTTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 TAACACTACTGGTTATATTGACCATGCCGCTTTTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1292 NA +3420 PHIX174 + 5386 5157 5156 5192 36 AAAATGCTCACAATGACAAATCTGTCCACGGAGTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAAATGCTCACAATGACAAATCTGTCCACGGAGTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5156 NA +3447 PHIX174 + 5386 772 771 807 36 GCGCAGGAAACACTGACGTTCTTACTGACGCAGAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCGCAGGAAACACTGACGTTCTTACTGACGCAGAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 771 NA +3402 PHIX174 + 5386 1224 1223 1259 36 GTCACGTTTATGGTGAACAGTGGATTAAGTTCATGA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCACGTTTATGGTGAACAGTGGATTAAGTTCATGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1223 NA +3138 PHIX174 + 5386 1544 1543 1576 33 CGCCAAATGACGACTTCTACCACATCTATTGAC HWI-EAS91_1_306UPAAXX - 36 4 3 36 1 0 33 33 CGCCAAATGACGACTTCTACCACATCTATTGAC ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 1540 NA +3420 PHIX174 + 5386 4840 4839 4875 36 GCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4839 NA +3329 PHIX174 + 5386 204 203 238 35 GATTCTGTCAAAAACTGACGCGTTGGATGAGGAGA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GATTCTGTCAAAAACTGACGCGTTGGATGAGGAGA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 203 NA +3197 PHIX174 + 5386 2481 2480 2516 36 ACCTGTTTTACAGACACCTAAAGCTACATCGTCAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ACCTGTTTTAAAGACACCTAAAGCTACATCGTCAAC ..........x......................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2480 NA +3429 PHIX174 + 5386 332 331 367 36 AAGAGCGTGGATTACTATCTGAGTCCGATGCTGTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGAGCGTGGATTACTATCTGAGTCCGATGCTGTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 331 NA +3393 PHIX174 + 5386 3978 3977 4013 36 TTTATGGTTCGTTCTTATTACCCTTCTGAATGTCAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTTATGGTTCGTTCTTATTACCCTTCTGAATGTCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3977 NA +3420 PHIX174 + 5386 4865 4864 4900 36 GCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4864 NA +3411 PHIX174 + 5386 3271 3270 3306 36 GAAAGGATACTCGTGATTATCTTGCTGCTGCATTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAAAGGATACTCGTGATTATCTTGCTGCTGCATTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3270 NA +3411 PHIX174 + 5386 4174 4173 4209 36 TTGAGTTCGATAATGGTGATATGTATGTTGACGGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTGAGTTCGATAATGGTGATATGTATGTTGACGGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4173 NA +3429 PHIX174 + 5386 2738 2737 2773 36 GCCATGATGGTGGTTATTATACCGTCAAGGACTGTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCATGATGGTGGTTATTATACCGTCAAGGACTGTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2737 NA +3329 PHIX174 + 5386 2703 2702 2737 35 CCCGACTGCCTATGATGTTTATCCTTTGAATGGTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CCCGACTGCCTATGATGTTTATCCTTTGAATGGTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2701 NA +3456 PHIX174 + 5386 2943 2942 2978 36 ATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2942 NA +3411 PHIX174 + 5386 3861 3860 3896 36 AATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3860 NA +3429 PHIX174 + 5386 3093 3092 3128 36 GCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3092 NA +3420 PHIX174 + 5386 3151 3150 3186 36 GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3150 NA +3393 PHIX174 + 5386 1193 1192 1228 36 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1192 NA +3456 PHIX174 + 5386 157 156 192 36 CGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 156 NA +3429 PHIX174 + 5386 4792 4791 4827 36 GGACACTTCCTACAGGTAGCGTTGACCCTAATTTTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGACACTTCCTACAGGTAGCGTTGACCCTAATTTTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4791 NA +3402 PHIX174 + 5386 3849 3848 3884 36 GATACTTGGAACAATTTCTGGAAAGACGGTAAAGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATACTTGGAACAATTTCTGGAAAGACGGTAAAGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3848 NA +3329 PHIX174 + 5386 2743 2742 2777 35 GATGGTGGTTATTATACCGTCAAGGACTGTGTGAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GATGGTGGTTATTATACCGTCAAGGACTGTGTGAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2741 NA +3465 PHIX174 + 5386 3015 3014 3050 36 GCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3014 NA +3438 PHIX174 + 5386 1091 1090 1126 36 ATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1090 NA +3329 PHIX174 + 5386 798 797 832 35 GACGCAGAAGAAAACGTGCGTCAAAAATTACGTGC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GACGCAGAAGAAAACGTGCGTCAAAAATTACGTGC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 796 NA +3420 PHIX174 + 5386 4866 4865 4901 36 CAAAATACGTGGCCTTATGGTTACAGTATGCCCATC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAAAATACGTGGCCTTATGGTTACAGTATGCCCATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4865 NA +3411 PHIX174 + 5386 4404 4403 4439 36 GAACGCCCTCTTAAGGATATTCGCGATGAGTATAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAACGCCCTCTTAAGGATATTCGCGATGAGTATAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4403 NA +3384 PHIX174 + 5386 1655 1654 1690 36 GTTATTTCTTCATTTGGAGGTAAAACCTCTTATGAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTTATTTCTTCATTTGGAGGTAAAACCTCTTATGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1654 NA +3420 PHIX174 + 5386 5162 5161 5197 36 GCTCACAATGACAAATCTGTCCACGGAGTGCTTAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTCACAATGACAAATCTGTCCACGGAGTGCTTAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5161 NA +3320 PHIX174 + 5386 1249 1248 1283 35 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1247 NA +3402 PHIX174 + 5386 3261 3260 3296 36 GCTGATAAAGGAAAGGATACTCGTGATTATCTTGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGATAAAGGAAAGGATACTCGTGATTATCTTGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3260 NA +3429 PHIX174 + 5386 3096 3095 3131 36 ATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3095 NA +3384 PHIX174 + 5386 2811 2810 2846 36 tGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2810 NA +3465 PHIX174 + 5386 3459 3458 3494 36 GCTGGCATTCAGTCGGCGACTTCACGCCAGAATACG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGGCATTCAGTCGGCGACTTCACGCCAGAATACG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3458 NA +3320 PHIX174 + 5386 789 788 823 35 GTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 788 NA +3420 PHIX174 + 5386 3699 3698 3734 36 GCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3698 NA +3206 PHIX174 + 5386 2641 2640 2676 36 GCCGACCCTAAATTTTTTGCCTGTTTGGTTCGCTTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCGACCCTAAATTTTTTGCCTGTTTGGTTCTCTTT ...............................x.... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2640 NA +3393 PHIX174 + 5386 305 304 340 36 GTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 304 NA +3329 PHIX174 + 5386 3066 3065 3100 35 GGCATGGGTGATGCTGGTATTAAATCTGCCATTCA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GGCATGGGTGATGCTGGTATTAAATCTGCCATTCA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 3065 NA +3375 PHIX174 + 5386 4428 4427 4463 36 GATGAGTATAATTACCCCAAAAAGAAAGGTATTAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATGAGTATAATTACCCCAAAAAGAAAGGTATTAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4427 NA +3429 PHIX174 + 5386 1461 1460 1496 36 AAGATGATGCTCGTTATGGTTTCCGTTGCTGCCATC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGATGATGCTCGTTATGGTTTCCGTTGCTGCCATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1460 NA +3402 PHIX174 + 5386 4000 3999 4035 36 CTTCTGAATGTCACGCTGATTATTTTGACTTTGAGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTCTGAATGTCACGCTGATTATTTTGACTTTGAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3999 NA +3429 PHIX174 + 5386 220 219 255 36 GACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 219 NA +3233 PHIX174 + 5386 3194 3193 3229 36 GCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTTCT .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 3193 NA +3124 PHIX174 + 5386 1266 1265 1300 35 GTGTTAATGCCACTCCTCTCCCGACTGTTAACACT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GTGTTAATGCCACTCCTCTCCCGACTGTTAACTCT ................................x.. 35M 34/35 97.1% 35/36 97.2% 0/35 0.0% 1265 NA +3429 PHIX174 + 5386 4596 4595 4631 36 GCTGACGACCGATTAGAGGCGTTTTATGATAATCCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCTGACGACCGATTAGAGGCGTTTTATGATAATCCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4595 NA +3311 PHIX174 + 5386 1172 1171 1206 35 GGCCTTGCTATTGACTCTACTGTAGACATTTTTAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GGCCTTGCTATTGACTCTACTGTAGACATTTTTAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1170 NA +3420 PHIX174 + 5386 636 635 671 36 GCTTATTATGTTCATCCCGTCAACATTCAAACGGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCTTATTATGTTCATCCCGTCAACATTCAAACGGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 635 NA +3393 PHIX174 + 5386 2873 2872 2908 36 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2872 NA +3474 PHIX174 + 5386 1014 1013 1049 36 AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1013 NA +3438 PHIX174 + 5386 2689 2688 2724 36 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2688 NA +3206 PHIX174 + 5386 2148 2147 2183 36 GCGTACTTATTCGCCACCATGATTATGACCAGTGTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCGTACTTATTCGCCACCATGATTATTACCAGTGTT ..........................x......... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2147 NA +3347 PHIX174 + 5386 5191 5190 5225 35 GCTTAATCCAACTTACCAAGCTGGGTTACGACGCG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GCTTAATCCAACTTACCAAGCTGGGTTACGACGCG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 5190 NA +3402 PHIX174 + 5386 1590 1589 1625 36 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1589 NA +3142 PHIX174 + 5386 571 570 605 35 GTACGCTGGACTTTGTGGGATACCCTCGCTTTCCT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GTACGCTGTACTTTGTGGGATACCCTCGCTTTCCT ........x.......................... 35M 34/35 97.1% 35/36 97.2% 0/35 0.0% 570 NA +3438 PHIX174 + 5386 242 241 277 36 GGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 241 NA +3429 PHIX174 + 5386 338 337 373 36 GTGGATTACTATCTGAGTCCGATGCTGTTCAACCAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTGGATTACTATCTGAGTCCGATGCTGTTCAACCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 337 NA +3278 PHIX174 + 5386 1112 1111 1147 36 GCTGGCGACTCCTTCGAGATGGACGCCGTTGGCGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGGCGACTCCTTCGAGATGGACGCCGTTTGCGCT ..............................x..... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 1111 NA +3247 PHIX174 + 5386 1254 1253 1287 34 TCATGAAGGATGGTGTTAATGCCACTCCTCTCCC HWI-EAS91_1_306UPAAXX - 36 3 2 36 1 0 34 34 TCATGAAGGATGGTGTTAATGCCACTCCTCTCCC .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 1251 NA +3242 PHIX174 + 5386 2970 2969 3005 36 GCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGGTGGCGCCATGTCTAAATTTTTTGGAGGCGGT .......................x............ 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2969 NA +3420 PHIX174 + 5386 5151 5150 5186 36 GGGATGAAAATGCTCACAATGACAAATCTGTCCACG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGGATGAAAATGCTCACAATGACAAATCTGTCCACG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5150 NA +3411 PHIX174 + 5386 348 347 383 36 ATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 347 NA +3365 PHIX174 + 5386 4888 4887 4922 35 ACAGTATGCCCATCGCAGTTCGCTACACGCAGGAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ACAGTATGCCCATCGCAGTTCGCTACACGCAGGAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4886 NA +3111 PHIX174 + 5386 3972 3971 4004 33 GGCTTTTTTATGGTTCGTTCTTATTACCCTTCT HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 GGCTTTTTTATGGTTCGTTCTTATTACCCTTCT ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 3971 NA +3438 PHIX174 + 5386 5033 5032 5068 36 GTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5032 NA +3320 PHIX174 + 5386 1552 1551 1586 35 GACGACTTCTACCACATCTATTGACATTATGGGTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GACGACTTCTACCACATCTATTGACATTATGGGTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1550 NA +3429 PHIX174 + 5386 1764 1763 1799 36 AAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1763 NA +3438 PHIX174 + 5386 1529 1528 1564 36 GAGACTGAGCTTTCTCGCCAAATGACGACTTCTACC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGACTGAGCTTTCTCGCCAAATGACGACTTCTACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1528 NA +3438 PHIX174 + 5386 4950 4949 4985 36 CCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4949 NA +3447 PHIX174 + 5386 4484 4483 4519 36 GGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTaC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4483 NA +3429 PHIX174 + 5386 177 176 212 36 CTTTGCGACCTTTCGCCATCAACTAACGATTCTGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTTGCGACCTTTCGCCATCAACTAACGATTCTGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 176 NA +3429 PHIX174 + 5386 2577 2576 2612 36 GATGGATACATCTGTCAACGCCGCTAATCAGGTTGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATGGATACATCTGTCAACGCCGCTAATCAGGTTGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2576 NA +3456 PHIX174 + 5386 156 155 191 36 GCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 155 NA +3429 PHIX174 + 5386 227 226 262 36 TGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 226 NA +3465 PHIX174 + 5386 574 573 609 36 CGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 573 NA +3438 PHIX174 + 5386 3021 3020 3056 36 GGTGGCATTCAAGGTGATGTGCTTGCTACCGATAAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTGGCATTCAAGGTGATGTGCTTGCTACCGATAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3020 NA +3438 PHIX174 + 5386 3731 3730 3766 36 GAATCAGCGGTATGGCTCTTCTCATATTGGCGCTAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAATCAGCGGTATGGCTCTTCTCATATTGGCGCTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3730 NA +3420 PHIX174 + 5386 2436 2435 2471 36 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2435 NA +3411 PHIX174 + 5386 2710 2709 2745 36 GCCTATGATGTTTATCCTTTGAATGGTCGCCATGAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCTATGATGTTTATCCTTTGAATGGTCGCCATGAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2709 NA +3079 PHIX174 + 5386 4228 4227 4262 35 TTCGTGATGAGTTTGTATCTGTTACTGAGAAGTTA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTCGTGATGAGTTTGTATCTGTTACTGATAAGTTA ............................x...... 35M 34/35 97.1% 35/36 97.2% 0/35 0.0% 4227 NA +3202 PHIX174 + 5386 4606 4605 4639 34 GATTAGAGGCGTTTTATGATAATCCCAATGCTTT HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 GATTAGAGGCGTTTTATGATAATCCCAATGCTTT .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 4605 NA +3465 PHIX174 + 5386 2766 2765 2801 36 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2765 NA +3256 PHIX174 + 5386 582 581 615 34 TTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTG HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 TTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTG .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 581 NA +3474 PHIX174 + 5386 1144 1143 1179 36 CGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1143 NA +3465 PHIX174 + 5386 1514 1513 1549 36 GCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1513 NA +3393 PHIX174 + 5386 1657 1656 1692 36 TATTTCTTCATTTGGAGGTAAAACCTCTTATGACGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TATTTCTTCATTTGGAGGTAAAACCTCTTATGACGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1656 NA +3411 PHIX174 + 5386 2071 2070 2106 36 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2070 NA +3438 PHIX174 + 5386 3250 3249 3285 36 GCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3249 NA +3179 PHIX174 + 5386 1982 1981 2017 36 GAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAAATTTCTATGAATGATGTTTTCCGTTCTGGTGAT ..............x..................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 1981 NA +3393 PHIX174 + 5386 2217 2216 2252 36 AGGTTAAATTTAATGTGACCGTTTATCGCAATCTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGGTTAAATTTAATGTGACCGTTTATCGCAATCTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2216 NA +3115 PHIX174 + 5386 3480 3479 3514 35 TCACGCCAGAATACGAAAGACCAGGTATATGCACA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 TCACGACAGAATACGAAAGACCAGGTATATGCACA .....x............................. 35M 34/35 97.1% 35/36 97.2% 0/35 0.0% 3478 NA +3438 PHIX174 + 5386 1690 1689 1725 36 CGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1689 NA +3420 PHIX174 + 5386 1082 1081 1117 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1081 NA +3402 PHIX174 + 5386 4282 4281 4317 36 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4281 NA +3229 PHIX174 + 5386 4136 4135 4169 34 GCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAG HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 GCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAG .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 4135 NA +3384 PHIX174 + 5386 4414 4413 4449 36 TTAAGGATATTCGCGATGAGTATAATTACCCCAAAA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTAAGGATATTCGCGATGAGTATAATTACCCCAAAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4413 NA +3393 PHIX174 + 5386 3988 3987 4023 36 GTTCTTATTACCCTTCTGAATGTCACGCTGATTATT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTTCTTATTACCCTTCTGAATGTCACGCTGATTATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3987 NA +3456 PHIX174 + 5386 4203 4202 4238 36 GACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4202 NA +3215 PHIX174 + 5386 4098 4097 4133 36 ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGCTTGGCTTCAATAAGCAGATGGATAACCGCATC ............x....................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4097 NA +3338 PHIX174 + 5386 4307 4306 4341 35 TATTAATAACACTATAGACCACCGCCCCGAAGGGG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TATTAATAACACTATAGACCACCGCCCCGAAGGGG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4306 NA +3347 PHIX174 + 5386 1521 1520 1555 35 TTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1520 NA +3429 PHIX174 + 5386 945 944 980 36 TAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 944 NA +3411 PHIX174 + 5386 1575 1574 1610 36 ACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1574 NA +3438 PHIX174 + 5386 2689 2688 2724 36 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2688 NA +3447 PHIX174 + 5386 2099 2098 2134 36 GAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2098 NA +3483 PHIX174 + 5386 524 523 559 36 TACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 523 NA +3438 PHIX174 + 5386 1252 1251 1287 36 GTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1251 NA +3242 PHIX174 + 5386 4916 4915 4951 36 GCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGTCC .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4915 NA +3188 PHIX174 + 5386 3828 3827 3863 36 GGTATTGATAAAGCTGTTGCCGATACTTGGAACAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTATTGATAAAGCTGTTGCCGATACTTGTAACAAT .............................x...... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 3827 NA +3411 PHIX174 + 5386 3756 3755 3791 36 ATTGGCGCTACTGCAAAGGATATTTCTAATGTCGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTGGCGCTACTGCAAAGGATATTTCTAATGTCGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3755 NA +3492 PHIX174 + 5386 1116 1115 1151 36 GCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1115 NA +3334 PHIX174 + 5386 3099 3098 3134 36 CAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAAGGCTCTAATGTTCCTAACCCTGATGGGGCCGCC ............................:....... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 3098 NA +3393 PHIX174 + 5386 3082 3081 3117 36 GTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3081 NA +3393 PHIX174 + 5386 613 612 648 36 TTGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 612 NA +3447 PHIX174 + 5386 4936 4935 4971 36 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4935 NA +3393 PHIX174 + 5386 289 288 324 36 TCACATTTTGTTCATGGTAGAGATTCTCTTGTTGAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCACATTTTGTTCATGGTAGAGATTCTCTTGTTGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 288 NA +3438 PHIX174 + 5386 4690 4689 4725 36 GCAAGGCTAATGATTCACACGCCGACTGCTATCAGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAAGGCTAATGATTCACACGCCGACTGCTATCAGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4689 NA +3420 PHIX174 + 5386 3520 3519 3555 36 AGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3519 NA +3393 PHIX174 + 5386 3858 3857 3893 36 AACAATTTCTGGAAAGACGGTAAAGCTGATGGTATT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AACAATTTCTGGAAAGACGGTAAAGCTGATGGTATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3857 NA +3438 PHIX174 + 5386 3344 3343 3379 36 TTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3343 NA +3019 PHIX174 + 5386 4672 4671 4707 36 TTCTTGCTGCCGAGGGTCGCAAGGCTAATGATTCAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTCTTGCTGCCGAGGGTCGCAAGGCTATTGTTTCAC ...........................x..x..... 36M 34/36 94.4% 36/36 100.0% 0/36 0.0% 4671 NA +3411 PHIX174 + 5386 1800 1799 1835 36 AGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1799 NA +3420 PHIX174 + 5386 5077 5076 5112 36 GGAACAACTCACTAAAAACCAAGCTGTCGCTACTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGAACAACTCACTAAAAACCAAGCTGTCGCTACTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5076 NA +3347 PHIX174 + 5386 2763 2762 2797 35 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2761 NA +3402 PHIX174 + 5386 4282 4281 4317 36 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4281 NA +3188 PHIX174 + 5386 1203 1202 1238 36 TTACTTTTTATGTCCCTCATCGTCACGTTTATGGTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTACTTTTTATGTCCCTCATCGTCACGTTTATGTTG .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 1202 NA +3420 PHIX174 + 5386 5107 5106 5142 36 TACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5106 NA +3320 PHIX174 + 5386 5168 5167 5202 35 AATGACAAATCTGTCCACGGAGTGCTTAATCCAAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 AATGACAAATCTGTCCACGGAGTGCTTAATCCAAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 5166 NA +3224 PHIX174 + 5386 2956 2955 2991 36 TTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTGCTTCTGCTCTTGCTTGTGGCGCCATGTCTAAAT .................x.................. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2955 NA +3215 PHIX174 + 5386 624 623 659 36 GCTGCCGTCATTGCTTATTATGTTCATCCCGTCAAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGCCGTCATTGCTTATTATGTTCATCCCTTCAAC ..............................x..... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 623 NA +3329 PHIX174 + 5386 1877 1876 1911 35 CCGCCTACTGCGACTAAAGAGATTCAGTACCTTAA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CCGCCTACTGCGACTAAAGAGATTCAGTACCTTAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1875 NA +3429 PHIX174 + 5386 1214 1213 1249 36 GTCCCTCATCGTCACGTTTATGGTGAACAGTGGATT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCCCTCATCGTCACGTTTATGGTGAACAGTGGATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1213 NA +3010 PHIX174 + 5386 558 557 593 36 GCTTGCGTTTATGGTACGCTGGACTTTGTGGGATAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTTGCGTTTATGGTACGCTGGACTTTTTGTGATAC ...........................x..x..... 36M 34/36 94.4% 36/36 100.0% 0/36 0.0% 557 NA +3411 PHIX174 + 5386 3629 3628 3664 36 TACTCAAGCTCAAACGGCTGGTCAGTATTTTACCAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TACTCAAGCTCAAACGGCTGGTCAGTATTTTACCAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3628 NA +3320 PHIX174 + 5386 2254 2253 2288 35 GACCACTCGCGATTCAATCATGACTTCGTGATAAA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GACCACTCGCGATTCAATCATGACTTCGTGATAAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2253 NA +3420 PHIX174 + 5386 2479 2478 2514 36 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2478 NA +3474 PHIX174 + 5386 3001 3000 3036 36 GCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3000 NA +3356 PHIX174 + 5386 4667 4666 4701 35 TATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4666 NA +3438 PHIX174 + 5386 3464 3463 3499 36 CATTCAGTCGGCGACTTCACGCCAGAATACGAAAGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CATTCAGTCGGCGACTTCACGCCAGAATACGAAAGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3463 NA +3338 PHIX174 + 5386 4911 4910 4945 35 TACACGCAGGACGCTTTTTCACGTTCTGGTTGGTT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TACACGCAGGACGCTTTTTCACGTTCTGGTTGGTT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4910 NA +3197 PHIX174 + 5386 2222 2221 2257 36 AAATTTAATGTGACCGTTTATCGCAATCTGCCGACC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAATTTAATGTGAACGTTTATCGCAATCTGCCGACC .............x...................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2221 NA +3338 PHIX174 + 5386 646 645 680 35 TTCATCCCGTCAACATTCAAACGGCCTGTCTCATC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTCATCCCGTCAACATTCAAACGGCCTGTCTCATC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 645 NA +3366 PHIX174 + 5386 1364 1363 1399 36 AAGCATTTGTTTCAGGGTTATTTGAATATCTATAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGCATTTGTTTCAGGGTTATTTGAATATCTATAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1363 NA +3320 PHIX174 + 5386 1613 1612 1647 35 ACTGACCAAGAACGTGATTACTTCATGCAGCGTTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ACTGACCAAGAACGTGATTACTTCATGCAGCGTTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1611 NA +3347 PHIX174 + 5386 1045 1044 1079 35 TTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1044 NA +3411 PHIX174 + 5386 1588 1587 1623 36 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1587 NA +3420 PHIX174 + 5386 339 338 374 36 TGGATTACTATCTGAGTCCGATGCTGTTCAACCACT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGGATTACTATCTGAGTCCGATGCTGTTCAACCACT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 338 NA +3447 PHIX174 + 5386 4937 4936 4972 36 TGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4936 NA +3202 PHIX174 + 5386 4344 4343 4377 34 GAAAAATGGTTTTTAGAGAACGAGAAGACGGTTA HWI-EAS91_1_306UPAAXX - 36 3 2 36 1 0 34 34 GAAAAATGGTTTTTAGAGAACGAGAAGACGGTTA .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 4341 NA +3420 PHIX174 + 5386 3674 3673 3709 36 CAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3673 NA +3438 PHIX174 + 5386 4945 4944 4980 36 TGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4944 NA +3474 PHIX174 + 5386 1946 1945 1981 36 GGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1945 NA +3215 PHIX174 + 5386 2662 2661 2697 36 TGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCGACT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCTACT ................................x... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2661 NA +3447 PHIX174 + 5386 3313 3312 3348 36 TTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3312 NA +3438 PHIX174 + 5386 727 726 762 36 GTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 726 NA +3447 PHIX174 + 5386 555 554 590 36 GAGGCTTGCGTTTATGGTACGCTGGACTTTGTGGGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAGGCTTGCGTTTATGGTACGCTGGACTTTGTGGGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 554 NA +3384 PHIX174 + 5386 485 484 520 36 TGATTTCGATTTTCTGACGAGTAACAAAGTTTGGAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGATTTCGATTTTCTGACGAGTAACAAAGTTTGGAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 484 NA +3384 PHIX174 + 5386 318 317 353 36 TGTTGACATTTTAAAAGAGCGTGGATTACTATCTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGTTGACATTTTAAAAGAGCGTGGATTACTATCTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 317 NA +3429 PHIX174 + 5386 910 909 945 36 TAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 909 NA +3447 PHIX174 + 5386 4936 4935 4971 36 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4935 NA +3375 PHIX174 + 5386 5005 5004 5040 36 TCTATGTGGCTAAATACGTTAACAAAAAGTCAGATA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCTATGTGGCTAAATACGTTAACAAAAAGTCAGATA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5004 NA +3143 PHIX174 + 5386 1379 1378 1414 36 GGTTATTTGAATATCTATAACAACTATTTTAAAGCG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTTATTTGAATATCTATAACAACTATTTTAAATCG .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 1378 NA +3233 PHIX174 + 5386 3168 3167 3203 36 GGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTAAAGGACTTCTTGAAGGTACGTTGCAGTCTGGC ..............................x..... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 3167 NA +3138 PHIX174 + 5386 2738 2737 2770 33 GCCATGATGGTGGTTATTATACCGTCAAGGACT HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 GCCATGATGGTGGTTATTATACCGTCAAGGACT ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 2737 NA +3429 PHIX174 + 5386 4099 4098 4134 36 TGCTTGGCTTCCATAAGCAGATGGATAACCGCATCA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGCTTGGCTTCCATAAGCAGATGGATAACCGCATCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4098 NA +3429 PHIX174 + 5386 2574 2573 2609 36 TCAGATGGATACATCTGTCAACGCCGCTAATCAGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCAGATGGATACATCTGTCAACGCCGCTAATCAGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2573 NA +3429 PHIX174 + 5386 2453 2452 2488 36 TCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2452 NA +3438 PHIX174 + 5386 5179 5178 5214 36 TGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5178 NA +3015 PHIX174 + 5386 3778 3777 3811 34 TTTCTAATGTCGTCACTGATGCTGCTTCTGGTGT HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 TTTCTAATGTCGTCACTGATGCTGCTTCTGTTGT ..............................x... 34M 33/34 97.1% 34/36 94.4% 0/34 0.0% 3777 NA +3420 PHIX174 + 5386 1843 1842 1878 36 TGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1842 NA +3420 PHIX174 + 5386 1082 1081 1117 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1081 NA +3438 PHIX174 + 5386 1728 1727 1763 36 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1727 NA +3188 PHIX174 + 5386 4637 4636 4672 36 TTTGCGTGACTATTTTCGTGATATTGGTCGTATGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTTGCGTGACTATTTTCGTGATATTGTTCGTATGGT ..........................x......... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4636 NA +3420 PHIX174 + 5386 2225 2224 2260 36 TTTAATGTGACCGTTTATCGCAATCTGCCGACCACT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTTAATGTGACCGTTTATCGCAATCTGCCGACCACT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2224 NA +3384 PHIX174 + 5386 3368 3367 3403 36 CGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3367 NA +3456 PHIX174 + 5386 3311 3310 3346 36 GCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3310 NA +3420 PHIX174 + 5386 5039 5038 5074 36 TATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5038 NA +3420 PHIX174 + 5386 2059 2058 2094 36 TTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2058 NA +3411 PHIX174 + 5386 388 387 423 36 TCATGAGTCAAGTTACTGAACAATCCGTACGTTTCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCATGAGTCAAGTTACTGAACAATCCGTACGTTTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 387 NA +3420 PHIX174 + 5386 2754 2753 2789 36 TTATACCGTCAAGGACTGTGTGACTATTGACGTCCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTATACCGTCAAGGACTGTGTGACTATTGACGTCCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2753 NA +3420 PHIX174 + 5386 2128 2127 2163 36 TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2127 NA +3438 PHIX174 + 5386 154 153 189 36 TTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 153 NA +3447 PHIX174 + 5386 3617 3616 3652 36 GCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3616 NA +3420 PHIX174 + 5386 2728 2727 2763 36 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2727 NA +3456 PHIX174 + 5386 2775 2774 2810 36 GACTATTGACGTCCTTCCCCGTACGCCGGGCAATAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GACTATTGACGTCCTTCCCCGTACGCCGGGCAATAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2774 NA +3411 PHIX174 + 5386 4217 4216 4252 36 TGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4216 NA +3293 PHIX174 + 5386 4619 4618 4653 35 TTATGATAATCCCAATGCTTTGCGTGACTATTTTC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTATGATAATCCCAATGCTTTGCGTGACTATTTTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4618 NA +3338 PHIX174 + 5386 411 410 445 35 TCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 410 NA +3329 PHIX174 + 5386 2730 2729 2764 35 GAATGGTCGCCATGATGGTGGTTATTATACCGTCA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GAATGGTCGCCATGATGGTGGTTATTATACCGTCA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2729 NA +3383 PHIX174 + 5386 521 520 555 35 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 520 NA +3402 PHIX174 + 5386 4260 4259 4295 36 TTAATGGATGAATTGGCACAATGCTACAATGTGCTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTAATGGATGAATTGGCACAATGCTACAATGTGCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4259 NA +3411 PHIX174 + 5386 1662 1661 1697 36 CTTCATTTGGAGGTAAAACCTCTTATGACGCTGACA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTCATTTGGAGGTAAAACCTCTTATGACGCTGACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1661 NA +3465 PHIX174 + 5386 533 532 568 36 CTCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGTTTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGTTTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 532 NA +3320 PHIX174 + 5386 195 194 229 35 TCAACTAACGATTCTGTCAAAAACTGACGCGTTGG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TCAACTAACGATTCTGTCAAAAACTGACGCGTTGG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 194 NA +3429 PHIX174 + 5386 2239 2238 2274 36 TTATCGCAATCTGCCGACCACTCGCGATTCAATCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTATCGCAATCTGCCGACCACTCGCGATTCAATCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2238 NA +3224 PHIX174 + 5386 1313 1312 1348 36 GACCATGCCGCTTTTCTTGGCACGATTAACCCTGAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACCATGCCGCTTTTCTTGGCACGATTAACCCTTAT .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 1312 NA +3402 PHIX174 + 5386 1545 1544 1580 36 GCCAAATGACGACTTCTACCACATCTATTGACATTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCCAAATGACGACTTCTACCACATCTATTGACATTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1544 NA +3438 PHIX174 + 5386 557 556 592 36 GGCTTGCGTTTATGGTACGCTGGACTTTGTGGGATA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGCTTGCGTTTATGGTACGCTGGACTTTGTGGGATA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 556 NA +3411 PHIX174 + 5386 1249 1248 1284 36 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1248 NA +3197 PHIX174 + 5386 4174 4173 4209 36 TTGAGTTCGATAATGGTGATATGTATGTTGACGGCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTGAGTTCGATAATGGTGATATGTATGTTGACGTCC .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4173 NA +3302 PHIX174 + 5386 614 613 648 35 TGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 613 NA +3420 PHIX174 + 5386 206 205 241 36 TTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 205 NA +3411 PHIX174 + 5386 4981 4980 5016 36 CTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4980 NA +3215 PHIX174 + 5386 1702 1701 1737 36 TCCTTTACTTGTCATGCGCTCTAATCTCTGGGCATC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCCTTTACTTGTCATGCGCTCTAATCTCTGTGCATC ..............................x..... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 1701 NA +3302 PHIX174 + 5386 2321 2320 2355 35 TAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2320 NA +3338 PHIX174 + 5386 4088 4087 4122 35 TCAATCCCCAATGCTTGGCTTCCATAAGCAGATGG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TCAATCCCCAATGCTTGGCTTCCATAAGCAGATGG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4087 NA +3456 PHIX174 + 5386 900 899 935 36 TGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 899 NA +3429 PHIX174 + 5386 1155 1154 1190 36 TTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1154 NA +3438 PHIX174 + 5386 3587 3586 3622 36 TTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3586 NA +3420 PHIX174 + 5386 3787 3786 3822 36 TCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3786 NA +3311 PHIX174 + 5386 3834 3833 3868 35 GATAAAGCTGTTGCCGATACTTGGAACAATTTCTG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GATAAAGCTGTTGCCGATACTTGGAACAATTTCTG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 3833 NA +3393 PHIX174 + 5386 989 988 1024 36 TGAGGATAAATTATGTCTAATATTCAAACTGGCGCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGAGGATAAATTATGTCTAATATTCAAACTGGCGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 988 NA +3429 PHIX174 + 5386 3712 3711 3747 36 TAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3711 NA +3402 PHIX174 + 5386 1207 1206 1242 36 TTTTTATGTCCCTCATCGTCACGTTTATGGTGAACA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTTTTATGTCCCTCATCGTCACGTTTATGGTGAACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1206 NA +3447 PHIX174 + 5386 2116 2115 2151 36 TCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2115 NA +3438 PHIX174 + 5386 660 659 695 36 ATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 659 NA +3420 PHIX174 + 5386 1536 1535 1571 36 AGCTTTCTCGCCAAATGACGACTTCTACCACATCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGCTTTCTCGCCAAATGACGACTTCTACCACATCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1535 NA +3329 PHIX174 + 5386 2112 2111 2146 35 TCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 TCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2110 NA +3402 PHIX174 + 5386 2816 2815 2851 36 ATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2815 NA +3429 PHIX174 + 5386 5176 5175 5211 36 ATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5175 NA +3447 PHIX174 + 5386 3472 3471 3507 36 CGGCGACTTCACGCCAGAATACGAAAGACCAGGTAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGGCGACTTCACGCCAGAATACGAAAGACCAGGTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3471 NA +3465 PHIX174 + 5386 1948 1947 1983 36 CGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1947 NA +3293 PHIX174 + 5386 2212 2211 2246 35 TAGTCAGGTTAAATTTAATGTGACCGTTTATCGCA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TAGTCAGGTTAAATTTAATGTGACCGTTTATCGCA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2211 NA +3284 PHIX174 + 5386 3817 3816 3851 35 ATATTTTTCATGGTATTGATAAAGCTGTTGCCGAT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 ATATTTTTCATGGTATTGATAAAGCTGTTGCCGAT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 3816 NA +3393 PHIX174 + 5386 3563 3562 3598 36 GTCTATTATGGAAAACACCAATCTTTCCAAGCAACA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTCTATTATGGAAAACACCAATCTTTCCAAGCAACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3562 NA +3347 PHIX174 + 5386 4198 4197 4232 35 ATGTTGACGGCCATAAGGCTGCTTCTGACGTTCGT HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ATGTTGACGGCCATAAGGCTGCTTCTGACGTTCGT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4196 NA +3329 PHIX174 + 5386 2907 2906 2941 35 CCAGCCACTTAAGTGAGGTGATTTATGTTTGGTGC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CCAGCCACTTAAGTGAGGTGATTTATGTTTGGTGC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2905 NA +3347 PHIX174 + 5386 2843 2842 2877 35 TTACCGCTACTAAATGCCGCGGATTGGTTTCGCTG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTACCGCTACTAAATGCCGCGGATTGGTTTCGCTG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2842 NA +3251 PHIX174 + 5386 4374 4373 4409 36 GTTACGCAGTTTTGCCGCAAGCTGGCTGCTGAACGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTACGCAGTTTTGCCGCAAGCTGGCTGCTGTACGC ...............................x.... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4373 NA +3347 PHIX174 + 5386 1940 1939 1974 35 ATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1938 NA +3411 PHIX174 + 5386 5073 5072 5108 36 GAATGGAACAACTCACTAAAAACCAAGCTGTCGCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAATGGAACAACTCACTAAAAACCAAGCTGTCGCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5072 NA +3375 PHIX174 + 5386 4434 4433 4469 36 TATAATTACCCCAAAAAGAAAGGTATTAAGGATGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TATAATTACCCCAAAAAGAAAGGTATTAAGGATGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4433 NA +3347 PHIX174 + 5386 1274 1273 1308 35 GCCACTCCTCTCCCGACTGTTAACACTACTGGTTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GCCACTCCTCTCCCGACTGTTAACACTACTGGTTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1272 NA +3393 PHIX174 + 5386 4440 4439 4475 36 TACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4439 NA +3411 PHIX174 + 5386 3920 3919 3955 36 GTCAGGATTGACACCCTCCCAATTGTATGTTTTCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCAGGATTGACACCCTCCCAATTGTATGTTTTCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3919 NA +3347 PHIX174 + 5386 4587 4586 4621 35 CTCACGTTGGCTGACGACCGATTAGAGGCGTTTTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CTCACGTTGGCTGACGACCGATTAGAGGCGTTTTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4585 NA +3438 PHIX174 + 5386 1707 1706 1742 36 TACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1706 NA +3420 PHIX174 + 5386 1582 1581 1617 36 GGGTCTGCAAGCTGCTTATGCTAATTTGCATACTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGGTCTGCAAGCTGCTTATGCTAATTTGCATACTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1581 NA +3411 PHIX174 + 5386 1588 1587 1623 36 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1587 NA +3166 PHIX174 + 5386 1180 1179 1213 34 TATTGACTCTACTGTAGACATTTTTACTTTTTAT HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 TATTGACTCTACTGTAGACATTTTTACTTTTTAT .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 1179 NA +3420 PHIX174 + 5386 2698 2697 2733 36 ACCCTCCCGACTGCCTATGATGTTTATCCTTTGAAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ACCCTCCCGACTGCCTATGATGTTTATCCTTTGAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2697 NA +3438 PHIX174 + 5386 3167 3166 3202 36 TGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3166 NA +3420 PHIX174 + 5386 2289 2288 2324 36 AGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2288 NA +3402 PHIX174 + 5386 441 440 476 36 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 440 NA +3465 PHIX174 + 5386 1012 1011 1047 36 TCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1011 NA +3447 PHIX174 + 5386 161 160 196 36 GCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 160 NA +3347 PHIX174 + 5386 1165 1164 1199 35 GCGTCGTGGCCTTGCTATTGACTCTACTGTAGACA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GCGTCGTGGCCTTGCTATTGACTCTACTGTAGACA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1163 NA +3179 PHIX174 + 5386 5010 5009 5045 36 GTGGCTAAATACGTTAACAAAAAGTCAGATATGGAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTGGATAAATACGTTAACAAAAAGTCAGATATGGAC ....x............................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 5009 NA +3420 PHIX174 + 5386 2286 2285 2321 36 AAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2285 NA +3411 PHIX174 + 5386 801 800 836 36 GCAGAAGAAAACGTGCGTCAAAAATTACGTGCaGAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCAGAAGAAAACGTGCGTCAAAAATTACGTGCAGAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 800 NA +3411 PHIX174 + 5386 1587 1586 1622 36 TGCAAGCTGCTTATGCTAATTTGCATACTGACCAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGCAAGCTGCTTATGCTAATTTGCATACTGACCAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1586 NA +3438 PHIX174 + 5386 4578 4577 4613 36 TTTGACACTCTCACGTTGGCTGACGACCGATTAGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTTGACACTCTCACGTTGGCTGACGACCGATTAGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4577 NA +3411 PHIX174 + 5386 3570 3569 3605 36 ATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3569 NA +3411 PHIX174 + 5386 496 495 531 36 TTCTGACGAGTAACAAAGTTTGGATTGCTACTGACC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTCTGACGAGTAACAAAGTTTGGATTGCTACTGACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 495 NA +3402 PHIX174 + 5386 441 440 476 36 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 440 NA +3293 PHIX174 + 5386 4235 4234 4269 35 TGAGTTTGTATCTGTTACTGAGAAGTTAATGGATG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TGAGTTTGTATCTGTTACTGAGAAGTTAATGGATG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4234 NA +3411 PHIX174 + 5386 2293 2292 2328 36 TGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAATT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2292 NA +3311 PHIX174 + 5386 1579 1578 1613 35 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1577 NA +3242 PHIX174 + 5386 3332 3331 3367 36 TGGTGCTGATGCTTCCTCTGCTGGTATGGTTGACGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGGTGCTGATGCTTCCTCTGCTGGTATGGTTTACGC ...............................x.... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 3331 NA +3411 PHIX174 + 5386 4132 4131 4167 36 TCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4131 NA +3029 PHIX174 + 5386 1 0 32 32 GAGTTTTATCGCTTCCATGACGCAGAAGTTAA HWI-EAS91_1_306UPAAXX - 36 5 4 36 1 0 32 32 GAGTTTTATCGCTTCCATGACGCAGAAGTTAA ................................ 32M 32/32 100.0% 32/36 88.9% 0/32 0.0% -4 -32 +3366 PHIX174 + 5386 54 53 89 36 AGTCGAAAAATTATCTTGATAAAGCAGGAATTACTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGTCGAAAAATTATCTTGATAAAGCAGGAATTACTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 53 NA +3302 PHIX174 + 5386 680 679 714 35 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 678 NA +3429 PHIX174 + 5386 231 230 266 36 TGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 230 NA +3429 PHIX174 + 5386 4192 4191 4227 36 ATATGTATGTTGACGGCCATAAGGCTGCTTCTGACG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATATGTATGTTGACGGCCATAAGGCTGCTTCTGACG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4191 NA +3411 PHIX174 + 5386 1580 1579 1615 36 ATGGGTCTGCAAGCTGCTTATGCTAATTTGCATACT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGGGTCTGCAAGCTGCTTATGCTAATTTGCATACT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1579 NA +3393 PHIX174 + 5386 611 610 646 36 TGTTGAGTTTATTGCTGCCGTCATTGCTTATTATGT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGTTGAGTTTATTGCTGCCGTCATTGCTTATTATGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 610 NA +3338 PHIX174 + 5386 4532 4531 4566 35 GATGAATGCAATGCGACAGGCTCATGCTGATGGTT HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GATGAATGCAATGCGACAGGCTCATGCTGATGGTT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4530 NA +3097 PHIX174 + 5386 638 637 672 35 TTATTATGTTCATCCCGTCAACATTCAAACGGCCT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTATTATGTTCATCCCGTCAACATTCAAACTGCCT ..............................x.... 35M 34/35 97.1% 35/36 97.2% 0/35 0.0% 637 NA +3420 PHIX174 + 5386 1957 1956 1992 36 TTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1956 NA +3420 PHIX174 + 5386 2728 2727 2763 36 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2727 NA +3402 PHIX174 + 5386 3979 3978 4014 36 TTATGGTTCGTTCTTATTACCCTTCTGAATGTCACG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTATGGTTCGTTCTTATTACCCTTCTGAATGTCACG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3978 NA +3365 PHIX174 + 5386 540 539 574 35 GCTCGTCGCTGCGTTGAGGCTTGCGTTTATGGTAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GCTCGTCGCTGCGTTGAGGCTTGCGTTTATGGTAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 538 NA +3438 PHIX174 + 5386 1852 1851 1887 36 GTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1851 NA +3456 PHIX174 + 5386 4684 4683 4719 36 AGGGTCGCAAGGCTAATGATTCACACGCCGACTGCT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGGGTCGCAAGGCTAATGATTCACACGCCGACTGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4683 NA +3197 PHIX174 + 5386 3690 3689 3725 36 AAGGTTAGTGCTGAGGTTGACTTAGTTCATCAGCAA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 AAGGTTAGTGCTGAGGTTGACTTAGTTCATCATCAA ................................x... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 3689 NA +3447 PHIX174 + 5386 584 583 619 36 TGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 583 NA +3311 PHIX174 + 5386 3852 3851 3886 35 ACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 3850 NA +3329 PHIX174 + 5386 2857 2856 2891 35 TGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2856 NA +3438 PHIX174 + 5386 1728 1727 1763 36 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1727 NA +3356 PHIX174 + 5386 4658 4657 4692 35 TATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4657 NA +3420 PHIX174 + 5386 1721 1720 1756 36 TCTAATCTCTGGGCATCTGGCTATGATGTTGATGGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCTAATCTCTGGGCATCTGGCTATGATGTTGATGGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1720 NA +3429 PHIX174 + 5386 1954 1953 1989 36 TGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1953 NA +3438 PHIX174 + 5386 962 961 997 36 TAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 961 NA +3438 PHIX174 + 5386 2175 2174 2210 36 ACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2174 NA +3420 PHIX174 + 5386 2442 2441 2477 36 TAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2441 NA +3329 PHIX174 + 5386 5126 5125 5160 35 CAGAATCAGAATGAGCCGCAACTTCGGGATGAAAA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CAGAATCAGAATGAGCCGCAACTTCGGGATGAAAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 5124 NA +3474 PHIX174 + 5386 5206 5205 5241 36 CCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5205 NA +3393 PHIX174 + 5386 2879 2878 2914 36 ATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2878 NA +3402 PHIX174 + 5386 2004 2003 2039 36 TCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2003 NA +3411 PHIX174 + 5386 2154 2153 2189 36 TTATTCGCCACCATGATTATGACCAGTGTTTCCAGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTATTCGCCACCATGATTATGACCAGTGTTTCCAGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2153 NA +3356 PHIX174 + 5386 1093 1092 1127 35 TTCAACTACTCCGGTTATCGCTGGCGACTCCTTCG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTCAACTACTCCGGTTATCGCTGGCGACTCCTTCG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1092 NA +3447 PHIX174 + 5386 3127 3126 3162 36 AGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3126 NA +3420 PHIX174 + 5386 3544 3543 3579 36 AGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3543 NA +3070 PHIX174 + 5386 2508 2507 2542 35 ATCGTCAACGTTATATTTTGATAGTTTGACGGTTA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 ATCGTCAACGTTATATTTTGATAGTTTGACGTTTA ...............................x... 35M 34/35 97.1% 35/36 97.2% 0/35 0.0% 2507 NA +3420 PHIX174 + 5386 3600 3599 3635 36 CAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3599 NA +3420 PHIX174 + 5386 1734 1733 1769 36 CATCTGGCTATGATGTTGATGGAACTGACCAAACGT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CATCTGGCTATGATGTTGATGGAACTGACCAAACGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1733 NA +3129 PHIX174 + 5386 2655 2654 2687 33 TTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTC HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 TTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTC ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 2654 NA +3429 PHIX174 + 5386 2934 2933 2969 36 TTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2933 NA +3447 PHIX174 + 5386 1522 1521 1557 36 TCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1521 NA +3420 PHIX174 + 5386 4063 4062 4098 36 AGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4062 NA +3402 PHIX174 + 5386 3395 3394 3430 36 TAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3394 NA +3429 PHIX174 + 5386 409 408 444 36 AATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 AATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 408 NA +3420 PHIX174 + 5386 1867 1866 1902 36 TGTTCGTTTTCCGCCTACTGCGACTAAAGAGATTCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGTTCGTTTTCCGCCTACTGCGACTAAAGAGATTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1866 NA +3215 PHIX174 + 5386 3780 3779 3815 36 TCTAATGTCGTCACTGATGCTGCTTCTGGTGTGGTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCTAATGTCGTCACTGATGCTGCTTCTGGTGTGTTT .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 3779 NA +3420 PHIX174 + 5386 3982 3981 4017 36 TGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3981 NA +3402 PHIX174 + 5386 2815 2814 2850 36 TATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2814 NA +3402 PHIX174 + 5386 1242 1241 1277 36 AGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1241 NA +3356 PHIX174 + 5386 4659 4658 4693 35 ATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4657 NA +3420 PHIX174 + 5386 2436 2435 2471 36 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2435 NA +3383 PHIX174 + 5386 527 526 561 35 TGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 526 NA +3260 PHIX174 + 5386 4817 4816 4852 36 CCCTAATTTTGGTCGTCGGGTACGCAATCGCCGCCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CCCTAATTTGGGTCGTCGGGTACGCAATCGCCGCCA .........x.......................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4816 NA +3447 PHIX174 + 5386 2100 2099 2135 36 AAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 AAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2099 NA +3438 PHIX174 + 5386 2762 2761 2797 36 TCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2761 NA +3420 PHIX174 + 5386 4955 4954 4990 36 TGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4954 NA +3411 PHIX174 + 5386 3761 3760 3796 36 CGCTACTGCAAAGGATATTTCTAATGTCGTCACTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTACTGCAAAGGATATTTCTAATGTCGTCACTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3760 NA +3438 PHIX174 + 5386 4039 4038 4074 36 TCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4038 NA +3438 PHIX174 + 5386 4788 4787 4823 36 ATGCGGACACTTCCTACAGGTAGCGTTGACCCTAAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGCGGACACTTCCTACAGGTAGCGTTGACCCTAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4787 NA +3447 PHIX174 + 5386 1002 1001 1037 36 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1001 NA +3456 PHIX174 + 5386 4381 4380 4416 36 AGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4380 NA +3393 PHIX174 + 5386 4256 4255 4291 36 GAAGTTAATGGATGAATTGGCACAATGCTACAATGT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAAGTTAATGGATGAATTGGCACAATGCTACAATGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4255 NA +3329 PHIX174 + 5386 10 9 44 35 CGCTTCCATGACGCAGAAGTTAACACTTTCGGATA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CGCTTCCATGACGCAGAAGTTAACACTTTCGGATA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 8 NA +3411 PHIX174 + 5386 1579 1578 1614 36 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1578 NA +3411 PHIX174 + 5386 3862 3861 3897 36 ATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3861 NA +3420 PHIX174 + 5386 3278 3277 3313 36 TACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3277 NA +3420 PHIX174 + 5386 349 348 384 36 TCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 348 NA +3402 PHIX174 + 5386 4608 4607 4643 36 TTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4607 NA +3284 PHIX174 + 5386 3808 3807 3842 35 GTGTGGTTGATATTTTTCATGGTATTGATAAAGCT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GTGTGGTTGATATTTTTCATGGTATTGATAAAGCT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 3807 NA +3393 PHIX174 + 5386 286 285 321 36 GAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 285 NA +3429 PHIX174 + 5386 2850 2849 2885 36 TACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2849 NA +3302 PHIX174 + 5386 680 679 714 35 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 678 NA +3420 PHIX174 + 5386 4740 4739 4775 36 GAGTATGGTACAGCTAATGGCCGTCTTCATTTCCAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAGTATGGTACAGCTAATGGCCGTCTTCATTTCCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4739 NA +3338 PHIX174 + 5386 2467 2466 2501 35 CCAGCTTCTTCGGCACCTGTTTTACAGACACCTAA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CCAGCTTCTTCGGCACCTGTTTTACAGACACCTAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2465 NA +3193 PHIX174 + 5386 320 319 353 34 TTGACATTTTAAAAGAGCGTGGATTACTATCTGA HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 TTGACATTTTAAAAGAGCGTGGATTACTATCTGA .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 319 NA +3166 PHIX174 + 5386 1180 1179 1213 34 TATTGACTCTACTGTAGACATTTTTACTTTTTAT HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 TATTGACTCTACTGTAGACATTTTTACTTTTTAT .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 1179 NA +3474 PHIX174 + 5386 521 520 556 36 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 520 NA +3429 PHIX174 + 5386 333 332 368 36 AGAGCGTGGATTACTATCTGAGTCCGATGCTGTTCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGAGCGTGGATTACTATCTGAGTCCGATGCTGTTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 332 NA +3429 PHIX174 + 5386 2756 2755 2791 36 ATACCGTCAAGGACTGTGTGACTATTGACGTCCTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATACCGTCAAGGACTGTGTGACTATTGACGTCCTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2755 NA +3411 PHIX174 + 5386 4957 4956 4992 36 ATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTATA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTATA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4956 NA +3375 PHIX174 + 5386 72 71 107 36 ATAAAGCAGGAATTACTACTGCTTGTTTACGAATTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATAAAGCAGGAATTACTACTGCTTGTTTACGAATTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 71 NA +3393 PHIX174 + 5386 4249 4248 4284 36 TTACTGAGAAGTTAATGGATGAATTGGCACAATGCT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTACTGAGAAGTTAATGGATGAATTGGCACAATGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4248 NA +3438 PHIX174 + 5386 2592 2591 2627 36 CAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2591 NA +3420 PHIX174 + 5386 1974 1973 2009 36 CGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1973 NA +3429 PHIX174 + 5386 3540 3539 3575 36 AAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3539 NA +3402 PHIX174 + 5386 4130 4129 4165 36 CATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4129 NA diff -r efdb6fb5de34 -r 341d7c1c5c9a tools/sr_mapping/lastz_wrapper.py --- a/tools/sr_mapping/lastz_wrapper.py Fri Nov 27 10:53:50 2009 -0500 +++ b/tools/sr_mapping/lastz_wrapper.py Mon Nov 30 09:05:28 2009 -0500 @@ -1,6 +1,35 @@ #! /usr/bin/python """ +Runs Lastz +Written for Lastz v. 1.01.86. + +usage: lastz_wrapper.py [options] + --ref_name: The reference name to append to all output matches + --ref_source: Whether the reference is cached or from the history + --source_select: Whether to used pre-set or cached reference file + --input1: The name of the reference file if using history or reference base name if using cached + --input2: The reads file to align + --ref_sequences: The number of sequences in the reference file if using one from history + --pre_set_options: Which of the pre set options to use, if using pre-sets + --strand: Which strand of the read to search, if specifying all parameters + --seed: Seeding settings, if specifying all parameters + --gfextend: Whether to perform gap-free extension of seed hits to HSPs (high scoring segment pairs), if specifying all parameters + --chain: Whether to perform chaining of HSPs, if specifying all parameters + --transition: Number of transitions to allow in each seed hit, if specifying all parameters + --O: Gap opening penalty, if specifying all parameters + --E: Gap extension penalty, if specifying all parameters + --X: X-drop threshold, if specifying all parameters + --Y: Y-drop threshold, if specifying all parameters + --K: Threshold for HSPs, if specifying all parameters + --L: Threshold for gapped alignments, if specifying all parameters + --entropy: Whether to involve entropy when filtering HSPs, if specifying all parameters + --identity_min: Minimum identity (don't report matches under this identity) + --identity_max: Maximum identity (don't report matches above this identity) + --coverage: The minimum coverage value (don't report matches covering less than this) + --out_format: The format of the output file (sam, diffs, or tabular (general)) + --output: The name of the output file + --num_threads: The number of threads to run --lastzSeqsFileDir: Directory of local lastz_seqs.loc file """ import optparse, os, subprocess, shutil, sys, tempfile, threading @@ -45,36 +74,31 @@ def __main__(): #Parse Command Line parser = optparse.OptionParser() - parser.add_option( '', '--ref_name', dest='ref_name', help='' ) - parser.add_option( '', '--ref_source', dest='ref_source', help='' ) + parser.add_option( '', '--ref_name', dest='ref_name', help='The reference name to append to all output matches' ) + parser.add_option( '', '--ref_source', dest='ref_source', help='Whether the reference is cached or from the history' ) parser.add_option( '', '--ref_sequences', dest='ref_sequences', help='Number of sequences in the reference dataset' ) - parser.add_option( '', '--source_select', dest='source_select', help='' ) - parser.add_option( '', '--out_format', dest='out_format', help='' ) - parser.add_option( '', '--input1', dest='input1', help='' ) - parser.add_option( '', '--input2', dest='input2', help='' ) - parser.add_option( '', '--pre_set_options', dest='pre_set_options', help='' ) - parser.add_option( '', '--strand', dest='strand', help='' ) - parser.add_option( '', '--seed', dest='seed', help='' ) - parser.add_option( '', '--transition', dest='transition', help='' ) - parser.add_option( '', '--gfextend', dest='gfextend', help='' ) - parser.add_option( '', '--nogfextend', dest='nogfextend', help='' ) - parser.add_option( '', '--chain', dest='chain', help='' ) - parser.add_option( '', '--nochain', dest='nochain', help='' ) - parser.add_option( '', '--gapped', dest='gapped', help='' ) - parser.add_option( '', '--nogapped', dest='nogapped', help='' ) - parser.add_option( '', '--O', dest='O', help='' ) - parser.add_option( '', '--E', dest='E', help='' ) - parser.add_option( '', '--X', dest='X', help='' ) - parser.add_option( '', '--Y', dest='Y', help='' ) - parser.add_option( '', '--K', dest='K', help='' ) - parser.add_option( '', '--L', dest='L', help='' ) - parser.add_option( '', '--entropy', dest='entropy', help='' ) - parser.add_option( '', '--identity_min', dest='identity_min', help='' ) - parser.add_option( '', '--identity_max', dest='identity_max', help='' ) - parser.add_option( '', '--coverage', dest='coverage', help='' ) - parser.add_option( '', '--format', dest='format', help='' ) + parser.add_option( '', '--source_select', dest='source_select', help='Whether to used pre-set or cached reference file' ) + parser.add_option( '', '--input1', dest='input1', help='The name of the reference file if using history or reference base name if using cached' ) + parser.add_option( '', '--input2', dest='input2', help='The reads file to align' ) + parser.add_option( '', '--pre_set_options', dest='pre_set_options', help='Which of the pre set options to use, if using pre-sets' ) + parser.add_option( '', '--strand', dest='strand', help='Which strand of the read to search, if specifying all parameters' ) + parser.add_option( '', '--seed', dest='seed', help='Seeding settings, if specifying all parameters' ) + parser.add_option( '', '--transition', dest='transition', help='Number of transitions to allow in each seed hit, if specifying all parameters' ) + parser.add_option( '', '--gfextend', dest='gfextend', help='Whether to perform gap-free extension of seed hits to HSPs (high scoring segment pairs), if specifying all parameters' ) + parser.add_option( '', '--chain', dest='chain', help='Whether to perform chaining of HSPs, if specifying all parameters' ) + parser.add_option( '', '--O', dest='O', help='Gap opening penalty, if specifying all parameters' ) + parser.add_option( '', '--E', dest='E', help='Gap extension penalty, if specifying all parameters' ) + parser.add_option( '', '--X', dest='X', help='X-drop threshold, if specifying all parameters' ) + parser.add_option( '', '--Y', dest='Y', help='Y-drop threshold, if specifying all parameters' ) + parser.add_option( '', '--K', dest='K', help='Threshold for HSPs, if specifying all parameters' ) + parser.add_option( '', '--L', dest='L', help='Threshold for gapped alignments, if specifying all parameters' ) + parser.add_option( '', '--entropy', dest='entropy', help='Whether to involve entropy when filtering HSPs, if specifying all parameters' ) + parser.add_option( '', '--identity_min', dest='identity_min', help="Minimum identity (don't report matches under this identity)" ) + parser.add_option( '', '--identity_max', dest='identity_max', help="Maximum identity (don't report matches above this identity)" ) + parser.add_option( '', '--coverage', dest='coverage', help="The minimum coverage value (don't report matches covering less than this)" ) + parser.add_option( '', '--out_format', dest='format', help='The format of the output file (sam, diffs, or tabular (general))' ) parser.add_option( '', '--output', dest='output', help='The output file' ) - parser.add_option( '', '--num_threads', dest='num_threads', help='' ) + parser.add_option( '', '--num_threads', dest='num_threads', help='The number of threads to run' ) parser.add_option( '', '--lastzSeqsFileDir', dest='lastzSeqsFileDir', help='Directory of local lastz_seqs.loc file' ) ( options, args ) = parser.parse_args() @@ -88,9 +112,9 @@ set_options = '--%s' % options.pre_set_options # Prepare for user-specified options else: - set_options = '%s %s --%s --%s --%s --%s O=%s E=%s X=%s Y=%s K=%s L=%s %s' % \ - ( options.gfextend, options.chain, options.gapped, options.strand, - options.seed, options.transition, options.O, options.E, options.X, + set_options = '--%s --%s --gapped --%s --%s --%s O=%s E=%s X=%s Y=%s K=%s L=%s --%s' % \ + ( options.gfextend, options.chain, options.strand, options.seed, + options.transition, options.O, options.E, options.X, options.Y, options.K, options.L, options.entropy ) # Specify input2 and add [fullnames] modifier if output format is diffs if options.format == 'diffs': @@ -107,18 +131,21 @@ if options.ref_source == 'history': # Reference is a fasta dataset from the history, so split job across number of # sequences in the dataset - try: - error_msg = "The reference dataset is missing metadata, click the pencil icon in the history item and 'auto-detect' the metadata attributes." - ref_sequences = int( options.ref_sequences ) - if ref_sequences < 1: - stop_err( error_msg ) - except: - stop_err( error_msg ) - for seq in range( ref_sequences ): +# try: +# error_msg = "The reference dataset is missing metadata, click the pencil icon in the history item and 'auto-detect' the metadata attributes." +# ref_sequences = int( options.ref_sequences ) +# if ref_sequences < 1: +# stop_err( error_msg ) +# except: +# stop_err( error_msg ) + # Currently set up to work only for a fasta file with a single sequence + for seq in range(1): +# for seq in range( ref_sequences ): command = 'lastz %s%s %s %s --ambiguousn --nolaj --identity=%s..%s --coverage=%s --format=%s%s >> %s' % \ ( ref_name, options.input1, input2, set_options, options.identity_min, options.identity_max, options.coverage, format, tabular_fields, options.output ) commands.append( command ) + print command else: # Reference is a locally cached 2bit file, split job across number of chroms in 2bit file tbf = TwoBitFile( open( options.input1, 'r' ) ) @@ -127,7 +154,6 @@ ( ref_name, options.input1, chrom, input2, set_options, options.identity_min, options.identity_max, options.coverage, format, tabular_fields, options.output ) commands.append( command ) - tbf.close() job_runner = LastzJobRunner( int( options.num_threads ), commands ) if __name__=="__main__": __main__() diff -r efdb6fb5de34 -r 341d7c1c5c9a tools/sr_mapping/lastz_wrapper.xml --- a/tools/sr_mapping/lastz_wrapper.xml Fri Nov 27 10:53:50 2009 -0500 +++ b/tools/sr_mapping/lastz_wrapper.xml Mon Nov 30 09:05:28 2009 -0500 @@ -6,16 +6,18 @@ #else: --ref_name="None" #end if ---ref_source=$source.ref_source --source_select=$params.source_select --out_format=$out_format.value --input1=$source.input1 --input2=$input2 +--ref_source=$source.ref_source --source_select=$params.source_select --out_format=$out_format.value --input2=$input2 #if $source.ref_source=="history": +--input1=$source.input1 --ref_sequences=$input1.metadata.sequences #else: +--input1=$source.input1_2bit --ref_sequences="None" #end if #if $params.source_select=="pre_set": ---pre_set_options=${params.pre_set_options} --strand="None" --seed="None" --transition="None" --O="None" --E="None" --X="None" --Y="None" --K="None" --L="None" --entropy="None" +--pre_set_options=${params.pre_set_options} --strand="None" --seed="None" --gfextend="None" --chain="None" --transition="None" --O="None" --E="None" --X="None" --Y="None" --K="None" --L="None" --entropy="None" #else: ---pre_set_options="None" --strand=$params.strand --seed=$params.seed --transition=$params.transition --O=$params.O --E=$params.E --X=$params.X --Y=$params.Y --K=$params.K --L=$params.L --entropy=$params.entropy +--pre_set_options="None" --strand=$params.strand --seed=$params.seed --gfextend=$params.gfextend --chain=$params.chain --transition=$params.transition --O=$params.O --E=$params.E --X=$params.X --Y=$params.Y --K=$params.K --L=$params.L --entropy=$params.entropy #end if --identity_min=$min_ident --identity_max=$max_ident --coverage=$min_cvrg --format=$out_format --output=$output1 --num_threads=$num_threads --lastzSeqsFileDir=${GALAXY_DATA_INDEX_DIR} </command> @@ -27,7 +29,7 @@ <option value="history">in your history</option> </param> <when value="cached"> - <param name="input1" type="select" label="Using reference genome" help="If your genome of interest is not listed, contact the Galaxy team"> + <param name="input1_2bit" type="select" label="Using reference genome" help="If your genome of interest is not listed, contact the Galaxy team"> <options from_file="lastz_seqs.loc"> <column name="value" index="1" /> <column name="name" index="0" /> @@ -39,7 +41,7 @@ </when> </conditional> <param name="out_format" type="select" label="Output format"> - <option value="sam">SAM</option> + <option value="sam-">SAM</option> <option value="diffs">Polymorphisms</option> <option value="tabular">Tabular</option> </param> @@ -61,29 +63,28 @@ </when> <when value="full"> <param name="strand" type="select" label="Which strand to search?"> - <option value="--strand=both">Both</option> - <option value="--strand=plus">Search forward strand only (the one in the reference)</option> - <option value="--strand=minus">Search the reverse complement strand only (opposite of the reference)</option> + <option value="strand=both">Both</option> + <option value="strand=plus">Search forward strand only (the one in the reference)</option> + <option value="strand=minus">Search the reverse complement strand only (opposite of the reference)</option> </param> <param name="seed" type="select" label="Select seeding settings" help="allows you set word size and number of mismatches"> - <option value="--seed=12of19">Seed hits require a 19 bp word with matches in 12 specific positions</option> - <option value="--seed=14of22">Seed hits require a 22 bp word with matches in 14 specific positions</option> + <option value="seed=12of19">Seed hits require a 19 bp word with matches in 12 specific positions</option> + <option value="seed=14of22">Seed hits require a 22 bp word with matches in 14 specific positions</option> </param> <param name="transition" type="select" label="Select transition settings" help="affects the number of allowed transition substitutions"> - <option value="--transition">Allow one transition in each seed hit</option> - <option value="--transition2">Allow two transitions in a seed hit </option> - <option value="--notransition">Don't allow any transitions in seed hits</option> + <option value="transition">Allow one transition in each seed hit</option> + <option value="transition2">Allow two transitions in a seed hit </option> + <option value="notransition">Don't allow any transitions in seed hits</option> </param> - <param name="gfextend" type="boolean" truevalue="--gfextend" falsevalue="--nogfextend" label="Perform gap-free extension of seed hits to HSPs (high scoring segment pairs)?" /> - <param name="chain" type="boolean" truevalue="--chain" falsevalue="--nochain" label="Perform chaining of HSPs?" /> - <param name="gapped" type="boolean" truevalue="--gapped" falsevalue="--nogapped" label="Perform gapped alignment (instead of gap-free)?" /> + <param name="gfextend" type="boolean" truevalue="gfextend" falsevalue="nogfextend" label="Perform gap-free extension of seed hits to HSPs (high scoring segment pairs)?" /> + <param name="chain" type="boolean" truevalue="chain" falsevalue="nochain" label="Perform chaining of HSPs?" /> <param name="O" type="integer" size="5" value="400" label="Gap opening penalty"/> <param name="E" type="integer" size="5" value="30" label="Gap extension penalty"/> <param name="X" type="integer" size="5" value="910" label="X-drop threshold"/> <param name="Y" type="integer" size="5" value="9370" label="Y-drop threshold"/> <param name="K" type="integer" size="5" value="3000" label="Set the threshold for HSPs (ungapped extensions scoring lower are discarded)"/> <param name="L" type="integer" size="5" value="3000" label="Set the threshold for gapped alignments (gapped extensions scoring lower are discarded)"/> - <param name="entropy" type="boolean" truevalue="--entropy" falsevalue="--noentropy" label="Involve entropy when filtering HSPs?" /> + <param name="entropy" type="boolean" truevalue="entropy" falsevalue="noentropy" label="Involve entropy when filtering HSPs?" /> </when> </conditional> <conditional name="seq_name"> @@ -119,7 +120,7 @@ <param name="input2" value="b1.fasta" ftype="fasta" /> <param name="ref_source" value="history" /> <param name="input1" value="phiX.fasta" ftype="fasta" /> - <param name="out_format" value="sam" /> + <param name="out_format" value="sam-" /> <param name="source_select" value="pre_set" /> <param name="pre_set_options" value="yasra95short" /> <param name="how_to_name" value="No" /> @@ -129,7 +130,6 @@ <param name="num_threads" value="4" /> <output name="output1" file="lastz_wrapper_out1.sam" /> </test> -  <test> <param name="input2" value="b1.fasta" ftype="fasta" /> <param name="ref_source" value="history" /> @@ -171,7 +169,7 @@ <param name="min_cvrg" value="0" /> <param name="num_threads" value="4" /> <output name="output1" file="lastz_wrapper_out3.tabular" /> - </test> + </test> </tests> <help>

1 0