June 2010 - galaxy-dev - lists.galaxyproject.org

Error when dowloading large compressed data library
by padioleau 24 Jun '10

24 Jun '10

Dear Galaxy developers, When I try to download a large data library (from the Data Libraries tab) as any compressed file I encounter this error: ### Proxy Error The proxy server received an invalid response from an upstream server. The proxy server could not handle the request POST /galaxy_dev/ library_common/act_on_multiple_datasets. Reason: Error reading from remote server ### If I download file per file (no compression) it's working. Ismael -- Padioleau Ismael EMBL Heidelberg ( V205)

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query on upload files from file system path
by Vipin TS 23 Jun '10

23 Jun '10

Hi Galaxy team, I had updated my galaxy instance and tried to upload files from system path, but the pop-up menu doesn't shows the option. I can only see 3 options, they are: 1. Upload files 2. Upload directory of files 3. Import dataset from current history. happy to hear from your side. Vipin T S

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problems with Explorer(6) on WinXP when looking at "Saved Histories"
by Hans-Rudolf Hotz 23 Jun '10

23 Jun '10

Hi Last weekend, we successfully upgraded our galaxy installations to changeset "3894:837aabec314e" (according to the 'June 8, 2010 New Development News Brief'). We have no problems on Mac and Linux. However if you want to look at your "Saved Histories" on WinXP with Explorer(version 6) the browser freezes. This was not the case before, when we used "3545:2590120aed68" I just checked your public website (http://main.g2.bx.psu.edu/) using WinXP with Explorer and the browser freezes as well. Unfortunately, we (still) have a lot of Windows/Explorer users. So we are a little bit stuck at the moment, as I don't want to downgrade our Galaxy servers...(and it might take a while for our "PC support" to install Firefox) Thank you very much for your help Hans

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problems with install on OSX 10.5, Python 2.5.1
by Arlin Stoltzfus 23 Jun '10

23 Jun '10

Hello. I'm a Python newbie having problems trying to install galaxy on a Mac (PPC) running OSX 10.5.8 and Python 2.5.1. Do I need to upgrade my Python or OSX version? If not, below are the gory details for anyone who wants to help. Arlin ----------------------------- gory details (narrative description followed by transcript) --------------------- I've been through this several times and loaded various packages manually, and it all seems to come down to numpy 1.3.0 and bx-python 0.5.0. I installed more recent versions of these myself (numpy 1.4.1 and bx-python 0.6.0), and verified that they were installed correctly, but Galaxy seems to want specific versions that are not the current versions. I found a binary of numpy 1.3.0 but the installer would not let me install it on the grounds that it requires "System Python 2.5", which is confusing because thats what I thought I had. I haven't found bx-python 0.5.0 anywhere though I suppose I could just build an old release from the source repository. camel12:dev arlin$ uname -a Darwin camel12.umbi.umd.edu 9.8.0 Darwin Kernel Version 9.8.0: Wed Jul 15 16:57:01 PDT 2009; root:xnu-1228.15.4~1/RELEASE_PPC Power Macintosh camel12:dev arlin$ python --version Python 2.5.1 camel12:dev arlin$ hg clone http://bitbucket.org/galaxy/galaxy-dist (works fine) camel12:dev arlin$ cd galaxy-dist/ camel12:galaxy-dist arlin$ sudo sh setup.sh . . . (lots of messages) Fetched http://eggs.g2.bx.psu.edu/new/pexpect/pexpect-2.4-py2.5.egg Traceback (most recent call last): File "./scripts/fetch_eggs.py", line 31, in <module> c.resolve() # Only fetch eggs required by the config File "/Users/arlin/dev/galaxy-dist/lib/galaxy/eggs/__init__.py", line 284, in resolve egg.resolve() File "/Users/arlin/dev/galaxy-dist/lib/galaxy/eggs/__init__.py", line 172, in resolve r = pkg_resources .working_set.resolve( ( self.distribution.as_requirement(), ), env, self.fetch ) File "/Library/Python/2.5/site-packages/distribute-0.6.10-py2.5.egg/ pkg_resources.py", line 565, in resolve ) pkg_resources.DistributionNotFound: bx-python==0.5.0-dev-f74aec067563 ----------------------- end of gory details -------------------------------------- ------- Arlin Stoltzfus (arlin(a)umd.edu) Fellow, IBBR; Adj. Assoc. Prof., UMCP; Research Biologist, NIST IBBR, 9600 Gudelsky Drive, Rockville, MD tel: 240 314 6208; web: www.molevol.org

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NGS data library construction
by Terrence Barrette 23 Jun '10

23 Jun '10

Hi All, I'm working on building a local instance of the galaxy server. I've got the application up and its proxied behind a local apache. The base OS is redhat 5.5 kernel 2.6.18-194, python 2.6, on an hp dl385 with 8 cores and 20 GB of ram. The local drive is 1 TB SAS and I have a few TB of nfs space mounted as /exds/galaxy for holding the datafiles as a repository. I've got about 1 TB of solexa seq I want to make available to the various tools, bowtie, bwa, ... for analysis by the group but I'm a little uncertain how to proceed. It seems that I should make a file similar to faseq.loc in the tool-data folder that points to the individual files in a folder in the nfs space, but I'm not sure how that will make it available to the different tools that could use it and how to keep it a single copy as different users access it and choose from the fastq files to make datasets for analysis. In the galaxy-wiki it said to write to galaxy-dev(a)bx.psu.edu, on the galaxy site it links to galaxy-bugs(a)bx.psu.edu, at bitbucket I was just going to add this question as a issue. What is the preferred way to ask questions like this? Thanks Terry ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues

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Lunch job as connected user
by Mariette 23 Jun '10

23 Jun '10

Hi, We have galaxy working on our cluster and we require user to be logged in using our ldap server. However jobs are owned by root when runing on the cluster as this user lunch galaxy. How can we configure galaxy, so the connected user is the user who own the runing job ? thanks for your help, Jerome

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Running Galaxy behind Apache Proxy
by Michael Siebauer 23 Jun '10

23 Jun '10

Hey, I have a problem running Galaxy behind an Apache Proxy. I followed the ApacheProxy Tutorial: http://bitbucket.org/galaxy/galaxy-central/wiki/Config/ApacheProxy loaded the proxy, proxy_http and rewrite modules and added | <Directory /var/www/> RewriteEngine on RewriteRule ^(.*) http://localhost:8080$1 [P] |To the Apache configuration. When I try to access the Server via address http://bioapp07/ I get an "Internal Server Error" and the Galaxy Debug log shows: |Starting server in PID 1077. serving on 0.0.0.0:8080 view at http://127.0.0.1:8080 ---------------------------------------- Exception happened during processing of request from ('127.0.0.1', 52475) Traceback (most recent call last): File "/homes/galaxy/galaxy_dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py", line 1053, in process_request_in_thread self.finish_request(request, client_address) File "/usr/lib/python2.6/SocketServer.py", line 320, in finish_request self.RequestHandlerClass(request, client_address, self) File "/usr/lib/python2.6/SocketServer.py", line 615, in __init__ self.handle() File "/homes/galaxy/galaxy_dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py", line 432, in handle BaseHTTPRequestHandler.handle(self) File "/usr/lib/python2.6/BaseHTTPServer.py", line 329, in handle self.handle_one_request() File "/homes/galaxy/galaxy_dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py", line 427, in handle_one_request self.wsgi_execute() File "/homes/galaxy/galaxy_dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py", line 287, in wsgi_execute self.wsgi_start_response) File "/homes/galaxy/galaxy_dist/eggs/Paste-1.6-py2.6.egg/paste/urlmap.py", line 194, in __call__ path_info = self.normalize_url(path_info, False)[1] File "/homes/galaxy/galaxy_dist/eggs/Paste-1.6-py2.6.egg/paste/urlmap.py", line 118, in normalize_url "URL fragments must start with / or http:// (you gave %r)" % url) AssertionError: URL fragments must start with / or http:// (you gave 'HTTP/1.1') ----------------------------------------| Is there some other module missing, or what am I doing wrong? Ciao, Michael ;-)

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MySQL
by SHAUN WEBB 23 Jun '10

23 Jun '10

Hi, I am trying to set up a MySQL database on our computer cluster as a backend to Galaxy. I had no trouble with this conversion on our local server installation but now I am faced with an error. The full traceback is below, I'm not really sure what the problem is. If any one could give me some idea that would be great. Thanks Shaun Webb galaxy.model.migrate.check INFO 2010-06-04 16:16:40,360 No database, initializing Traceback (most recent call last): File "/exports/work/biology_cell_galaxy/galaxy/lib/galaxy/web/buildapp.py", line 61, in app_factory app = UniverseApplication( global_conf = global_conf, **kwargs ) File "/exports/work/biology_cell_galaxy/galaxy/lib/galaxy/app.py", line 28, in __init__ create_or_verify_database( db_url, self.config.database_engine_options ) File "/exports/work/biology_cell_galaxy/galaxy/lib/galaxy/model/migrate/check.py", line 67, in create_or_verify_database migrate_to_current_version( engine, db_schema ) File "/exports/work/biology_cell_galaxy/galaxy/lib/galaxy/model/migrate/check.py", line 108, in migrate_to_current_version changeset = schema.changeset( None ) File "/exports/work/biology_cell_galaxy/galaxy/eggs/py2.4-noplatform/sqlalchemy_migrate-0.5.4-py2.4.egg/migrate/versioning/schema.py", line 173, in changeset changeset = self.repository.changeset(database, start_ver, version) File "/exports/work/biology_cell_galaxy/galaxy/eggs/py2.4-noplatform/sqlalchemy_migrate-0.5.4-py2.4.egg/migrate/versioning/repository.py", line 170, in changeset changes = [self.version(v).script(database, op) for v in versions] File "/exports/work/biology_cell_galaxy/galaxy/eggs/py2.4-noplatform/sqlalchemy_migrate-0.5.4-py2.4.egg/migrate/versioning/version.py", line 161, in script assert ret is not None AssertionError -- The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336.

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Tarballs download links dead @ galaxy-central
by Pieter Neerincx 23 Jun '10

23 Jun '10

Hi, FYI: The links at http://bitbucket.org/galaxy/galaxy-central/wiki/GetGalaxy to get a Galaxy tarball are dead: ------------------------------------------------------------ 404 - Not Found We're sorry, but we couldn't find the page you were looking for. You can perhaps find what you're looking for by doing a search in the list of public repositories. If you feel that this is an error on our part, please send us an email with details about the error and what you were doing when it occurred. -- The Bitbucket team ------------------------------------------------------------ I can get Galaxy at http://bitbucket.org/galaxy/galaxy-dist/downloads though :) Cheers, Pi --------------------------------------------------------------- Biomolecular Mass Spectrometry & Proteomics group Utrecht University Visiting address: H.R. Kruyt building room O607 Padualaan 8 3584 CH Utrecht The Netherlands Mail address: P.O. box 80.082 3508 TB Utrecht The Netherlands phone: +31 6 143 66 783 email: pieter.neerincx(a)gmail.com skype: pieter.online ---------------------------------------------------------------

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Compressed File Storage
by Dennis Gascoigne 22 Jun '10

22 Jun '10

Hi guys; I was wondering how you are addressing the issues associated with compression;/decompression. We are using galaxy and instead it is inflating our raw storage budget by 500%. This is a major issue and we are trying to figure out how it can be fixed within the Galaxy framework. Any ideas? -- Dennis Gascoigne 0407 639 995 dennis.gascoigne(a)gmail.com

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