- galaxy-dev - lists.galaxyproject.org

The Galaxy release 18.05 is out
by Martin Čech 23 May '18

23 May '18

Dear Community, We are happy to release Galaxy 18.05. Many thanks to committers and you, the community. Enjoy the on time release and please let us know if you face any issues. The release notes are available at https://docs.galaxyproject.org/en/release_18.05/releases/18.05_announce.html Thanks for using Galaxy! Martin, on behalf of the Galaxy committers

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Workflow renaming problem for tool with multiple outputs (Galaxy version 17.09)
by Previti 23 May '18

23 May '18

Hi everybody, I'd like to fix this bug in our local galaxy instance...it's with regard to the erroneous renaming of output files, when there are multiple outputs available. The relevant ticket is the following: https://github.com/galaxyproject/galaxy/pull/5021 Unfortunately a quick update to the newest Galaxy version is not possible for us at the moment. Any tips on how I could do this? Thanks and best regards, Christopher Previti -- *Dr. Christopher Previti* Genomics and Proteomics Core Facility High Throughput Sequencing (W190) Bioinformatician German Cancer Research Center (DKFZ) Foundation under Public Law Im Neuenheimer Feld 580 69120 Heidelberg Germany Room: B2.102 (INF580/TP3) Phone: +49 6221 42-4661 christopher.previti(a)dkfz.de <http://www.dkfz.de/> www.dkfz.de <http://www.dkfz.de/> Management Board: Prof. Dr. Michael Baumann, Prof. Dr. Josef Puchta VAT-ID No.: DE143293537 Vertraulichkeitshinweis: Diese Nachricht ist ausschließlich für die Personen bestimmt, an die sie adressiert ist. Sie kann vertrauliche und/oder nur für den/die Empfänger bestimmte Informationen enthalten. Sollten Sie nicht der bestimmungsgemäße Empfänger sein, kontaktieren Sie bitte den Absender und löschen Sie die Mitteilung. Jegliche unbefugte Verwendung der Informationen in dieser Nachricht ist untersagt.

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Purging "Monitor installing repositories"
by Previti 23 May '18

23 May '18

Dear all, I've been having an issue with the "Monitor installing repositories" page. There is a whole list of tools that were not installed properly (usually they got interrupted for some reason) and they aren't being removed. Is there a way to purge this list manually? Thanks and best regards, Christopher Previti <http://dkfzgalaxy.inet.dkfz-heidelberg.de/admin_toolshed/monitor_repository…> -- *Dr. Christopher Previti* Genomics and Proteomics Core Facility High Throughput Sequencing (W190) Bioinformatician German Cancer Research Center (DKFZ) Foundation under Public Law Im Neuenheimer Feld 580 69120 Heidelberg Germany Room: B2.102 (INF580/TP3) Phone: +49 6221 42-4661 christopher.previti(a)dkfz.de <http://www.dkfz.de/> www.dkfz.de <http://www.dkfz.de/> Management Board: Prof. Dr. Michael Baumann, Prof. Dr. Josef Puchta VAT-ID No.: DE143293537 Vertraulichkeitshinweis: Diese Nachricht ist ausschließlich für die Personen bestimmt, an die sie adressiert ist. Sie kann vertrauliche und/oder nur für den/die Empfänger bestimmte Informationen enthalten. Sollten Sie nicht der bestimmungsgemäße Empfänger sein, kontaktieren Sie bitte den Absender und löschen Sie die Mitteilung. Jegliche unbefugte Verwendung der Informationen in dieser Nachricht ist untersagt.

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Text file busy and conda
by John Letaw 16 May '18

16 May '18

Hi all, I’m receiving the following error trying to access tools autoinstalled via conda: Fatal error: Exit code 126 () ~/galaxy/database/jobs_directory/000/38/tool_script.sh: line 41: ~/galaxy/database/dependencies/_conda/envs/mulled-v1-bb83f93efd111e042823e53ddfa74c32d81ba74cceca9445dfddfc9e940ff738/bin/samtools: Text file busy So, something is happening too fast I guess, meaning a process is attempting access while this is being created. Any ideas on how I can diagnose this? Not seeing any other errors floating around the logs. Maybe we are dealing with a lustre file locking issue? Thanks, John

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Trackster ownership issue
by Previti 16 May '18

16 May '18

Dear all, I'm having a strange issue with Trackster in our Galaxy implementation. Users seem to be able to upload files ("Add Datasets") from*all *histories of all users, regardless of ownership. ...is this something that has been observed before...? How do I stop this from happening? Thanks and best regards, Christopher -- *Dr. Christopher Previti* Genomics and Proteomics Core Facility High Throughput Sequencing (W190) Bioinformatician German Cancer Research Center (DKFZ) Foundation under Public Law Im Neuenheimer Feld 580 69120 Heidelberg Germany Room: B2.102 (INF580/TP3) Phone: +49 6221 42-4661 christopher.previti(a)dkfz.de <http://www.dkfz.de/> www.dkfz.de <http://www.dkfz.de/> Management Board: Prof. Dr. Michael Baumann, Prof. Dr. Josef Puchta VAT-ID No.: DE143293537 Vertraulichkeitshinweis: Diese Nachricht ist ausschließlich für die Personen bestimmt, an die sie adressiert ist. Sie kann vertrauliche und/oder nur für den/die Empfänger bestimmte Informationen enthalten. Sollten Sie nicht der bestimmungsgemäße Empfänger sein, kontaktieren Sie bitte den Absender und löschen Sie die Mitteilung. Jegliche unbefugte Verwendung der Informationen in dieser Nachricht ist untersagt.

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V18.01 'View all histories' doesn't work
by Ping Luo 15 May '18

15 May '18

I just installed Galaxy V18.01. I got an error when clicking 'View all histories' in the history tool bar The error says 'The requested URL /api/histories was not found on this server'. Is there anyone experiencing the same problem? Ping

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Re: [galaxy-dev] Tool to download galaxy datasets locally
by Nicola Soranzo 09 May '18

09 May '18

Dear Evan, you need to setup an environment variable for the tool to specify the directory where users are expected to save their files. One way to do that is: 1) create a directory called EXPORT_DIR_PREFIX in your tool dependencies directory (which by default is `database/dependencies/` ) 2) in this directory create a file called env.sh with a content similar to the following template: EXPORT_DIR_PREFIX=/PATH/WHERE/TO/SAVE/FILES; export EXPORT_DIR_PREFIX 3) create a symlink called `default` inside the EXPORT_DIR_PREFIX directory pointing to the same directory, e.g.: $ cd TOOL_DEPENDENCIES_DIR/EXPORT_DIR_PREFIX/ $ ln -s . default Alternatively, you could define the EXPORT_DIR_PREFIX environment variable in your config/job_conf.xml for the destination where the export_to_cluster tool is going to run (for an example, see `_JAVA_OPTIONS` in config/job_conf.xml.sample_advanced ), but this would define the environment variable also for all the other tools that use that destination (normally not a problem, but worth to mention). Cheers, Nicola On 05/05/18 15:04, Evan Clark wrote: > Have you ever seen this error with export to cluster? > > export_to_cluster.py: error: Directory prefix cannot be empty > > Nicola Soranzo wrote: >> >> Hi Evan, >> you can have a look at >> https://toolshed.g2.bx.psu.edu/view/earlhaminst/export_to_cluster/ >> <https://toolshed.g2.bx.psu.edu/view/earlhaminst/export_to_cluster/b97775e9f…> >> >> Hope that helps! >> >> Cheers, >> Nicola >> >> On 27/03/18 16:03, evan clark wrote: >>> >>> Has anyone attempted to write a tool that allows copying of galaxy >>> datasets locally, i.e. without downloading via http. We have scenario >>> where there are some additional tools available only via command line >>> and it would be very useful to be able to directly copy galaxy >>> datasets from the datasets folder. >>> ___________________________________________________________ >>> Please keep all replies on the list by using "reply all" >>> in your mail client. To manage your subscriptions to this >>> and other Galaxy lists, please use the interface at: >>> https://lists.galaxyproject.org/ >>> >>> To search Galaxy mailing lists use the unified search at: >>> http://galaxyproject.org/search/ >>

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ELIXIR Workshop for Galaxy training material and skills improvement
by Nicola Soranzo 08 May '18

08 May '18

Dear all, we'd like to invite you to the "ELIXIR Workshop for Galaxy training material and skills improvement", which will be held 21-23 May at the Earlham Institute in Norwich, UK: http://www.earlham.ac.uk/elixir-workshop-galaxy-training-material-and-skill… The Galaxy Training Network (GTN) has been working for several years with GOBLET and the ELIXIR Training Platform to create material and deliver training for scientists, developers and system administrators. A selection of 72 tutorials, developed by more than 60 contributors, is already available at http://training.galaxyproject.org/ In this workshop, we intend to improve the participants’ understanding of learning principles, training techniques and best practices for materials preparation. We will then work together to expand the existing collection of Galaxy training materials by covering more topics relevant to the ELIXIR use cases, add missing annotation and metadata and make the materials easily accessible. The workshop will start with a 1-day "Train the Trainer" course, similar to the Carpentries' Instructor Training and the ELIXIR-EXCELERATE Train the Trainer. Here Galaxy trainers will develop an insight into different learning styles, understand what makes a good trainer, and learn new approaches to training from experienced trainers. This course will help them to better contribute and shape the materials during the hackathon. This workshop is kindly sponsored by ELIXIR-UK and the ELIXIR Training Platform. Registration closes soon, hurry up! https://www.eiseverywhere.com/galaxy We look forward to seeing many of you in Norwich! Nicola -- Nicola Soranzo, Ph.D. Data Infrastructure & Algorithms group Earlham Institute Norwich Research Park, Norwich, NR4 7UZ, UK http://www.earlham.ac.uk/nicola-soranzo

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GCCBOSC 2018 Early Registration Ends this Friday (May 11)
by Dave Clements 07 May '18

07 May '18

Hi All, Just a reminder that early registration for the Galaxy Community's biggest annual gathering ends this Friday. You can also register for housing and childcare too. https://galaxyproject.org/events/gccbosc2018/register/ https://galaxyproject.org/events/gccbosc2018/venue/#housing https://galaxyproject.org/events/gccbosc2018/childcare/ Hope to see you there, Dave C on behalf of the GCCBOSC 2018 Organizing Committee -- https://gccbosc2018.sched.com/ https://galaxyproject.org/ https://usegalaxy.org/

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Best practices for utilising Unique Molecular Indexing (UMI's)
by Brooks, Tony 04 May '18

04 May '18

Hi We are currently seeing a number of methods that are utilising the power of unique molecular indexing. Unfortunately, there is no consensus on how libraries should be configured, and therefore no consensus for how to deal with them within Galaxy. Often libraries that have the UMI placed directly downstream of the first (i7) index, such as ones using the IDT xGen adapter set (https://www.idtdna.com/pages/products/next-generation-sequencing/adapters/x…) Sometimes UMI's exist in place of the second (i5) index (https://www.neb.com/nebnext-direct/nebnext-direct-for-target-enrichment) In both cases, the recommended workflows are convoluted and all the necessary tools do not currently exist in the toolshed (so that the datasets need to be taken out of galaxy, processed and reloaded). It is possible to use bcl2fastq to output the UMI as an additional fastq file, but this would then require me to create a dataset triplicate (not pair) which afaik we can't do (yet). A quick Google/toolshed search had me find UMI-Tools & Je-Suite which both exist in Galaxy. Both these tools assume the UMI is "in-line" (i.e. at the beginning of the read 1 or read2 - not its own read), extract/remove the UMI and place it in the read header, where it is then used further down the line to dedup the bam file. Does anyone know of any tools that would take the UMI from a separate fastq and use it to tag the headers of actual read data. Or alternatively, a tool that will paste the UMI tag onto the 5' end of the read fastq? And whether these steps can be done within Galaxy, or maybe prior to fastq upload? Anyone have a method/workflow for UMI's? Thanks in advance Tony

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