- galaxy-dev - lists.galaxyproject.org

Displaying genomic sequences in Trackster
by Naharajan Lakshmanaperumal 24 May '13

24 May '13

Dear all, We have our own galaxy instance and the idea is to have trackster enabled for users to be able to visualize NGS mapping. We were able to configure trackster in our instance and the visualization works fine. We have two questions regarding trackster: 1) We can't display genomic sequences in trackster. As per the tutorial, we set the location of the .2bit file in the twobit.loc file for the trackster to be able to display the genomic sequence but for some reason it doesn't display it. The name of the builds is the same in all places i.e) in ucsc/chrom/builds.txt and also in the .loc files. Any ideas on what else should be done? 2) While saving the visualization, there is always an error message saying "could not save visualization" and it doesn't seem to be a web browser issue. How do we then save the visualization? Thanks in advance, Naharajan

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XML files for MACS v1.4
by vijayalakshmi 23 May '13

23 May '13

HI all, I am trying to integrate MACS v1.4 into Galaxy. But I am unable to find the XML for the following MACS tools 1) elandexport2bed 2) elandmulti2bed 3) elandresult2bed 4) macs14 5) sam2bed 6) wignorm Any pointer/guidance is appreciated. Thanks, VJ.

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Suggestion for "Latest revision: installation errors"
by Peter Cock 23 May '13

23 May '13

Hi Greg, I think it would be useful to be a little more inclusive on the Tool Shed listing "Latest revision: installation errors" and include repositories with tools missing tests, e.g. http://testtoolshed.g2.bx.psu.edu/view/peterjc/ncbi_blast_plus/2d4757de6180 Some of the BLAST+ tools don't yet have tests (since they require functionality not yet supported), but at that revision at least there is also an installation error - which we're discussing here: http://lists.bx.psu.edu/pipermail/galaxy-dev/2013-May/014795.html On a related point, it would be good to have the RSS feeds mention installation errors. Thanks, Peter

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tool_dependencies inside tool_dependencies
by Björn Grüning 23 May '13

23 May '13

Hi, is there a general rule to handle dependencies inside of tool_dependencies.xml? Lets assume I write a matplotlib orphan tool_dependencies.xml file. matplotlib depends on numpy. Numpy has already a orphan definition. Is there a way to include numpy as dependency inside the matplotlib-definition, so that I did not need to fetch and compile numpy inside of matplotlib? I tried to specify it beforehand but that did not work. Thanks! Bjoern

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writing <command> tag in xml file for a C++ tool
by vijayalakshmi 23 May '13

23 May '13

Hi all, I want to include a new tool to galaxy. The tool is written in C++. How do I write the xml file to execute a cpp program? The tool that I am trying to integrate is https://code.google.com/p/bedtools/ I have the class file to be executed and the command to execute it. For example, 'IntersectBed' is my class file. The command to execute it is $intersectBed -a reads.bed -b genes.bed (reads.bed and genes.bed are input files provided by the user) How should I write the <command> tag in the xml file so as to execute the above command? Since C++ is complied as opposed to Python which is interpreted, I am not sure how to write the <command> tag. I am a newbie. I appreciate any guidance offered! Thanks, VJ.

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Hard time getting UCSC to work with our server
by Shane Sturrock 22 May '13

22 May '13

I'm having a pretty torrid time with this because we've had to implement active directory authentication for Apache. It works and users can log in with their AD accounts and that is all fine. The problems arise when I try and get UCSC to pick up a BAM file. The first problem is that although the Galaxy server is configured to for UCSC as a display server, putting the following into the Apache conf as recommended here http://wiki.galaxyproject.org/Admin/Config/Apache%20Proxy doesn't work. 1 <Location "/root/display_as"> 2 Satisfy Any 3 Order deny,allow 4 Deny from all 5 Allow from hgw1.cse.ucsc.edu 6 Allow from hgw2.cse.ucsc.edu 7 Allow from hgw3.cse.ucsc.edu 8 Allow from hgw4.cse.ucsc.edu 9 Allow from hgw5.cse.ucsc.edu 10 Allow from hgw6.cse.ucsc.edu 11 Allow from hgw7.cse.ucsc.edu 12 Allow from hgw8.cse.ucsc.edu 13 </Location> All that happens is you get 401 Authorization Required errors indicating that the Apache server isn't letting the UCSC servers non-authenticated access. Now, if I add the Allow from hgw etc into the <Location "/"> block then it gets through and then I hit the next problem which is that although I've mapped some read data against dm3 I'm getting failures inside Galaxy itself related to serving the bai file UCSC is requesting. Here's the galaxy_access log: 192.168.240.21 - shane [23/May/2013:15:22:24 +1200] "GET /display_application/d3a4d6a5256f2d9a/ucsc_bam/main HTTP/1.1" 302 406 hgw4.cse.ucsc.edu - - [23/May/2013:15:22:24 +1200] "GET /dataset/display_application?app_action=param&user_id=207edd50b8d72f8b&app_name=ucsc_bam&action_param=track&link_name=main&dataset_id=d3a4d6a5256f2d9a HTTP/1.0" 200 311 hgw4.cse.ucsc.edu - - [23/May/2013:15:22:29 +1200] "HEAD /dataset/display_application?app_action=data&user_id=207edd50b8d72f8b&app_name=ucsc_bam&action_param=galaxy_d3a4d6a5256f2d9a.bam&link_name=main&dataset_id=d3a4d6a5256f2d9a HTTP/1.0" 200 - hgw4.cse.ucsc.edu - - [23/May/2013:15:22:31 +1200] "HEAD /dataset/display_application?app_action=data&user_id=207edd50b8d72f8b&app_name=ucsc_bam&action_param=galaxy_d3a4d6a5256f2d9a.bam&link_name=main&dataset_id=d3a4d6a5256f2d9a.bai HTTP/1.0" 500 - hgw4.cse.ucsc.edu - - [23/May/2013:15:22:30 +1200] "GET /dataset/display_application?app_action=data&user_id=207edd50b8d72f8b&app_name=ucsc_bam&action_param=galaxy_d3a4d6a5256f2d9a.bam&link_name=main&dataset_id=d3a4d6a5256f2d9a HTTP/1.0" 200 472000 Note the 500 when requesting the bai file. This is what my manager.log is showing when this happens. 128.114.119.134 - - [23/May/2013:15:22:29 +1300] "HEAD /dataset/display_application?app_action=data&user_id=207edd50b8d72f8b&app_name=ucsc_bam&action_param=galaxy_d3a4d6a5256f2d9a.bam&link_name=main&dataset_id=d3a4d6a5256f2d9a HTTP/1.1" 200 3301004 "-" "genome.ucsc.edu/net.c" ---------------------------------------- Exception happened during processing of request from ('127.0.0.1', 60484) Traceback (most recent call last): File "/home/galaxy/galaxy-dist/eggs/Paste-1.7.5.1-py2.6.egg/paste/httpserver.py", line 1068, in process_request_in_thread self.finish_request(request, client_address) File "/usr/lib64/python2.6/SocketServer.py", line 322, in finish_request self.RequestHandlerClass(request, client_address, self) File "/usr/lib64/python2.6/SocketServer.py", line 618, in __init__ self.finish() File "/usr/lib64/python2.6/SocketServer.py", line 661, in finish self.wfile.flush() File "/usr/lib64/python2.6/socket.py", line 303, in flush self._sock.sendall(buffer(data, write_offset, buffer_size)) error: [Errno 32] Broken pipe ---------------------------------------- 128.114.119.134 - - [23/May/2013:15:22:30 +1300] "GET /dataset/display_application?app_action=data&user_id=207edd50b8d72f8b&app_name=ucsc_bam&action_param=galaxy_d3a4d6a5256f2d9a.bam&link_name=main&dataset_id=d3a4d6a5256f2d9a HTTP/1.1" 200 3301004 "-" "genome.ucsc.edu/net.c" 128.114.119.134 - - [23/May/2013:15:22:31 +1300] "HEAD /dataset/display_application?app_action=data&user_id=207edd50b8d72f8b&app_name=ucsc_bam&action_param=galaxy_d3a4d6a5256f2d9a.bam&link_name=main&dataset_id=d3a4d6a5256f2d9a.bai HTTP/1.1" 500 - "-" "genome.ucsc.edu/net.c" Error - <type 'exceptions.TypeError'>: Non-hexadecimal digit found ---------------------------------------- Exception happened during processing of request from ('127.0.0.1', 60486) Traceback (most recent call last): File "/home/galaxy/galaxy-dist/eggs/Paste-1.7.5.1-py2.6.egg/paste/httpserver.py", line 1068, in process_request_in_thread self.finish_request(request, client_address) File "/usr/lib64/python2.6/SocketServer.py", line 322, in finish_request self.RequestHandlerClass(request, client_address, self) File "/usr/lib64/python2.6/SocketServer.py", line 618, in __init__ self.finish() File "/usr/lib64/python2.6/SocketServer.py", line 661, in finish self.wfile.flush() File "/usr/lib64/python2.6/socket.py", line 303, in flush self._sock.sendall(buffer(data, write_offset, buffer_size)) error: [Errno 32] Broken pipe ---------------------------------------- ---------------------------------------- Exception happened during processing of request from ('127.0.0.1', 60485) Traceback (most recent call last): File "/home/galaxy/galaxy-dist/eggs/Paste-1.7.5.1-py2.6.egg/paste/httpserver.py", line 1068, in process_request_in_thread self.finish_request(request, client_address) File "/usr/lib64/python2.6/SocketServer.py", line 322, in finish_request self.RequestHandlerClass(request, client_address, self) File "/usr/lib64/python2.6/SocketServer.py", line 618, in __init__ self.finish() File "/usr/lib64/python2.6/SocketServer.py", line 661, in finish self.wfile.flush() File "/usr/lib64/python2.6/socket.py", line 303, in flush self._sock.sendall(buffer(data, write_offset, buffer_size)) error: [Errno 32] Broken pipe ---------------------------------------- And here's the text version in the browser (edited out our server's hostname though): URL: https://<ourgalaxyserver>/dataset/display_application?app_action=data&user_id=207edd50b8d72f8b&app_name=ucsc_bam&action_param=galaxy_d3a4d6a5256f2d9a.bam&link_name=main&dataset_id=d3a4d6a5256f2d9a.bai File '/home/galaxy/galaxy-dist/lib/galaxy/web/framework/middleware/error.py', line 149 in __call__ app_iter = self.application(environ, sr_checker) File '/home/galaxy/galaxy-dist/eggs/Paste-1.7.5.1-py2.6.egg/paste/recursive.py', line 84 in __call__ return self.application(environ, start_response) File '/home/galaxy/galaxy-dist/lib/galaxy/web/framework/middleware/remoteuser.py', line 91 in __call__ return self.app( environ, start_response ) File '/home/galaxy/galaxy-dist/eggs/Paste-1.7.5.1-py2.6.egg/paste/httpexceptions.py', line 633 in __call__ return self.application(environ, start_response) File '/home/galaxy/galaxy-dist/lib/galaxy/web/framework/base.py', line 125 in __call__ return self.handle_request( environ, start_response ) File '/home/galaxy/galaxy-dist/lib/galaxy/web/framework/base.py', line 182 in handle_request body = method( trans, **kwargs ) File '/home/galaxy/galaxy-dist/lib/galaxy/webapps/galaxy/controllers/dataset.py', line 727 in display_application data, user = decode_dataset_user( trans, dataset_id, user_id ) File '/home/galaxy/galaxy-dist/lib/galaxy/datatypes/display_applications/util.py', line 22 in decode_dataset_user dataset_id = trans.security.decode_id( dataset_hash ) File '/home/galaxy/galaxy-dist/lib/galaxy/web/security/__init__.py', line 60 in decode_id return int( self.id_cipher.decrypt( obj_id.decode( 'hex' ) ).lstrip( "!" ) ) File '/home/galaxy/galaxy_env/lib64/python2.6/encodings/hex_codec.py', line 42 in hex_decode output = binascii.a2b_hex(input) TypeError: Non-hexadecimal digit found I've been banging my head against this all day :-( Shane Sturrock

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Test Tool Shed still running tests marked as skip?
by Peter Cock 22 May '13

22 May '13

Hi Greg, There may be an issue with the new feature to mark a specific revision to skip tool tests. I ticked this earlier this week on this repository, yet the tests appear to have been run overnight (and failed as expected): http://testtoolshed.g2.bx.psu.edu/view/peterjc/tmhmm_and_signalp/2b35b5c4b7… Revision: 18:2b35b5c4b7f4 Regards, Peter

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<command> tag in xml file for a C++ tool
by vijayalakshmi 22 May '13

22 May '13

Hi all, I want to include a new tool to galaxy. The tool is written in C++. How do I write the xml file to execute a cpp program? The tool that I am trying to integrate is https://code.google.com/p/bedtools/ I have the class file to be executed and the command to execute it. For example, 'IntersectBed' is my class file. The command to execute it is $intersectBed -a reads.bed -b genes.bed (reads.bed and genes.bed are input files provided by the user) How should I write the <command> tag in the xml file so as to execute the above command? Since C++ is complied as opposed to Python which is interpreted, I am not sure how to write the <command> tag. I am a newbie. I appreciate any guidance offered! Thanks, VJ.

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nginx Proxy to VM problem
by Michael E. Cotterell 22 May '13

22 May '13

Hey everyone, I'm having a weird login problem (think it's cookie related) after setting up Galaxy on a virtual machine and trying to proxy_pass to it using nginx. It says I'm logged in, but when I get redirected to the front page I'm no longer logged in. When I visit subsequent pages I'm also not logged in. On the server, here is the relevant part of my nginx configuration (I'm going to setup static serving after I get the login issue resolved): upstream galaxy_app { server 192.168.1.11:8080; } server { ... location /galaxy { proxy_pass http://galaxy_app; proxy_set_header X-Forwarded-Host $host; proxy_set_header X-Forwarded-For $proxy_add_x_forwarded_for; } ... } The dots just mean that I have other things in there for the forwarding I do with other VMs. On the VM, in my universe_wsgi.ini file, I have made sure to set the following (just showing the parts I think are relevant): [server:main] port = 8080 host = 0.0.0.0 [filter:proxy-prefix] use = egg:PasteDeploy#prefix prefix = /galaxy [app:main] filter-with = proxy-prefix cookie_path = /galaxy I should note that I might end up changing this configuration to use a CNAME at some point (thus avoiding the prefix issue), but for now I'm totally lost as to why I'm not able to login using my current configuration. Any ideas? Thanks! Sincerely, Michael E. Cotterell Ph.D. Student in Computer Science, University of Georgia Instructor of Record, Graduate RA & TA, University of Georgia mepcotterell(a)gmail.com (mailto:mepcotterell@gmail.com) mepcott(a)uga.edu (mailto:mepcott@uga.edu) mec(a)cs.uga.edu (mailto:mec@cs.uga.edu) http://michaelcotterell.com/

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location of .loc-files/data tables -- bug or feature?
by Christian Hundsrucker 22 May '13

22 May '13

Hello, Galaxy developers! I do a bit of tweaking to a local galaxy instance. Thus, I just realized that subdirectories are not supported for the location of .loc-files/data tables. E.g. tool-data/somesub/my.loc is not recognized even if this location is correctly linked in the tool_data_table_conf.xml (<file path="tool-data/somesub/my.loc" />). Via the Galaxy-Admin interface and 'Server', 'View data tables registry' the .loc file is marked as a "Missing index file" - in the example the displayed location would be "tool-data/my.loc" (without the 'somesub' subdirectory). As soon as I store 'my.loc' directly in the tool-data directory galaxy runs smoothly again, even (!) if I keep the subdirectory in the file path in tool_data_table_conf.xml. Is galaxy meant to behave like this? If so, a word about it in the documentation would be really helpful! Best, Christian version: changeset: 8796:961ae35ba612 branch: stable tag: tip parent: 8794:1c7174911392 user: Nate Coraor <nate(a)bx.psu.edu> date: Fri Feb 08 11:23:04 2013 -0500

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