Dear Marcus:
If you are using Synechococcus sp. PCC 7002 sequences from the
Galaxy's microbial upload tool do this:
1. Simply select all sequences you want and click "Execute"
2. Use "Text Manipulation->Concatenate Queries" to concatenate all
sequences into a single dataset
3. Run MAQ
Let me know if you have further issues.
anton
On Jun 3, 2009, at 5:26 PM, Marcus Ludwig wrote:
Hi,
I want to map a set of sequences (derived from RNA) against a
bacterial
genome. Unfortunately, the genome of Synechococcus sp. PCC 7002
consists
of one chromosome and six plasmids. In MAQ I can chose only one DNA
entry for mapping. In local BLAST it was possible to generate
databases
consisting of several DNA entries to map the data.
Does anybody have experience in mapping transcription data against
complex genomes in Galaxy.
Best regards,
Marcus
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Anton Nekrutenko
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http://galaxyproject.org