July 2009 - galaxy-user - lists.galaxyproject.org

upgrading my local galaxy install
by Erick Antezana 03 Jul '09

03 Jul '09

Hi, I have upgraded my local install by getting the latest source like this: hg pull -u then I compared the *.sample.xml files against my *.xml files (tool_conf.xml). I have also upgraded my DB (from version 6 to 7). I have also launched : sh run_functional_tests.sh and I got a message: ... *** This program requires python version 2.5 or higher *** Current python version is: 2.4.3.final.0 ... I have python 2.4.3. Do I need to upgrade it? During the tests execution I saw the following error messages: ... Test uploading encode data ... ERROR ... ... Gencode Partition ( gencode_partition1 ) > Test-1 ... FAIL ... Stitch MAF blocks ( Interval_Maf_Merged_Fasta2 ) > Test-1 ... FAIL Stitch MAF blocks ( Interval_Maf_Merged_Fasta2 ) > Test-2 ... FAIL ... Summary Statistics ( Summary_Statistics1 ) > Test-1 ... FAIL ... BLAT ( blat_wrapper ) > Test-1 ... FAIL ... Fetch taxonomic representation ( Fetch Taxonomic Ranks ) > Test-1 ... FAIL Poisson two-sample test ( poisson2test ) > Test-1 ... FAIL Poisson two-sample test ( poisson2test ) > Test-2 ... FAIL Compute phastOdds score ( phastOdds_for_intervals ) > Test-1 ... FAIL LAV to BED ( lav_to_bed1 ) > Test-1 ... FAIL ... FAILED (failures=38, errors=5) the rest are OK, some errors and failures I guess are due to the fact that I do not have some tools installed (e.g. BLAT) Do I need to perform any other operation in order to upgrate my local install? (what about the "eggs"? Do I need to check for egg updates? how?) thanks, Erick

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Join EST and Refseq
by Massimo 01 Jul '09

01 Jul '09

Hi, I am trying to join a list of EST generated by UCSC Table (user track) with all bovine Refseq list generated as well from UCSC. The two do not share any common column and I cannot have the EST with the associated Refseq. As suggested by people at UCSC I have generated my list of custom track EST and then I have set the group mRNA and EST track, track mRNA, Table RefSeq gene (reGene) and I have done an intersection with all my ESTs (All refGene records that have any overlap with User Track ). I have then send everything to Galaxy and try to use the join two queries feature but I cannot obtain a unique table with both Refseq and EST accession numbers. I have contacted the people from UCSC but they said that there is a way through Galaxy..but I could not figured it out. Please, let me know if there is a way. Thanks in advance for your help! Massimo Bionaz PhD Post-doctoral researcher Institute for Genomic Biology, University of Illinois 366 Animal Sciences Laboratory 1207 W. Gregory Dr. Urbana, IL 61801 USA Office Tel: 1-217-333-6189 Fax: 1-217-333-8286

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Join EST and Refseq
by Massimo 01 Jul '09

01 Jul '09

Hi, I am trying to join a list of EST generated by UCSC Table (user track) with all bovine Refseq list generated as well from UCSC. The two do not share any common column and I cannot have the EST with the associated Refseq. As suggested by people at UCSC I have generated my list of custom track EST and then I have set the group mRNA and EST track, track mRNA, Table RefSeq gene (reGene) and I have done an intersection with all my ESTs (All refGene records that have any overlap with User Track ). I have then send everything to Galaxy and try to use the join two queries feature but I cannot obtain a unique table with both Refseq and EST accession numbers. I have contacted the people from UCSC but they said that there is a way through Galaxy..but I could not figured it out. Please, let me know if there is a way. Thanks in advance for your help! Massimo Bionaz PhD Post-doctoral researcher Institute for Genomic Biology, University of Illinois 366 Animal Sciences Laboratory 1207 W. Gregory Dr. Urbana, IL 61801 USA Office Tel: 1-217-333-6189 Fax: 1-217-333-8286

1 0

Join EST and Refseq
by Massimo 01 Jul '09

01 Jul '09

Hi, I am trying to join a list of EST generated by UCSC Table (user track) with all bovine Refseq list generated as well from UCSC. The two do not share any common column and I cannot have the EST with the associated Refseq. As suggested by people at UCSC I have generated my list of custom track EST and then I have set the group mRNA and EST track, track mRNA, Table RefSeq gene (reGene) and I have done an intersection with all my ESTs (All refGene records that have any overlap with User Track ). I have then send everything to Galaxy and try to use the join two queries feature but I cannot obtain a unique table with both Refseq and EST accession numbers. I have contacted the people from UCSC but they said that there is a way through Galaxy..but I could not figured it out. Please, let me know if there is a way. Thanks in advance for your help! Massimo Bionaz PhD Post-doctoral researcher Institute for Genomic Biology, University of Illinois 366 Animal Sciences Laboratory 1207 W. Gregory Dr. Urbana, IL 61801 USA Office Tel: 1-217-333-6189 Fax: 1-217-333-8286

1 0