March 2013 - galaxy-user - lists.galaxyproject.org

Help with Summary Statistics
by D. A. Cowart 23 May '14

23 May '14

Hello, I am attempting to use Galaxy to calculate the mean sequence read length and identify the range of read lengths for my 454 data. The data has already been organized and sorted by species. The format of the data is as follows: >HD4AU5D01BHBCQCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC >HD4AU5D01A093MCTCTGTCGCTCTGTCTCTCTTCTCTCTCTCTCTCTCT etc...for each species I have attempted to use the "Summary Statistics" button, however it appears to only be for numerical data and not sequence data. Is this tool/task available via Galaxy? Thank you, Dominique Cowart User name: dac330

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SNP finding
by Xiefan Fang 03 Dec '13

03 Dec '13

Dear galaxy users, We have done deep sequencing on some known genomic loci using Hiseq2000. I have already mapped the reads to the reference sequences by using Galaxy. In the next step, I want to find SNPs and calculate the SNP percentage within the reads. There are 500,000 to 1,000,000 reads per biological sample. Can I do it with galaxy? If not, is there other programs available in windows? Considering that I am not very familiar with programming. Thanks, Xiefan University of Florida

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barcode splitter files web based
by Ann Holtz-Morris, M.S. 11 Jul '13

11 Jul '13

HI, I'm trying to use the output files from barcode splitter. I know that the command line as some wrappers made that take the output files and puts them in the history. Is there anything similar for output files but for us biologists who don't do command line? J Thanks, Ann CONFIDENTIALITY NOTICE: This electronic message is intended to be for the use only of the named recipient, and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. Thank you.

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hello Newbie questions about galaxy and clustering
by leconte 19 Apr '13

19 Apr '13

hello I have installed an galaxy instance for purpose testing on a 8 CPU server without problem all run perfectly. I have made some galaxy xml wrapper to understant how galaxy calls program and other little things. Now I want to install a Galaxy server with a cluster configuration. Before I have tested HTCondor but I have no Idea how I can mix Galaxy and HTCondor. The perfect thing was somebody have already tested this type of condor. But I can satisfy myself with any other cluster type. What I don't understant in fact ( maybe my worst problem ) where lie all parts of this puzzle. I explain. I know that I need Galaxy. I know too I need a cluster ( in my case HTCondor ) I understand I need something between these 2 parts ... I think something like PBS or SGE but it's not totaly clear for me I'm search on galaxy mailling list archive ... but I just found 2 mails in 2008 :( has anybody made this conf or have a configuration ? greetings PS : sorry for my poor english :)

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Internal Server Error and slow to refresh History
by Gene Genome 11 Apr '13

11 Apr '13

Hi All, When I tried to download the filtered datasets from my History at Galaxy to my computer, I always got this error information: "Internal Server Error Galaxy was unable to sucessfully complete your request An error occurred. This may be an intermittent problem due to load or other unpredictable factors, reloading the page may address the problem. The error has been logged to our team. If you want to contact us about this error, please reference the following GURU MEDITATION: #8aa15caba14d4a3e85ce7ab069" What is the cause for this problem? In addition, It takes about 3 to 5 minutes to refresh my Histories or to view different Histories. However, it is very quick to refresh "Saved Histories". Is it normal? Thanks. Best, Jianguang

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Genomic Intervals Coverage: csv of 2nd dataset Identifiers
by Kumar, Charu Gupta 08 Apr '13

08 Apr '13

Hi, My question is about "operating on genomic intervals, Coverage" tool. I wanted a csv list of identifiers (c4) in the second dataset that cover each interval in the first dataset. How would I go about getting such a list? Thank you so much in advance. Best, Charu Charu Gupta Kumar, Ph.D.

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Peak Overlap Analysis, allowing space between "overlapping" peaks
by Eric Van Otterloo 08 Apr '13

08 Apr '13

Hello - I have been trying to find a solution to identify overlapping peaks between two ChIP-Seq datasets. I have used the "Intersect the intervals of two datasets" function, under the *Operate on Genomic Intervals* toolset - however, I would like to be able to specify a given distance between the peaks to still be counted as "overlapping", and this tool requires at least 1bp overlap between peaks to be counted. For example, even if two peaks are within 500bp of each other (but don't overlap) I would like to score this as overlapping and get the resulting genomic coordinates for downstream analysis. Thanks in advance for your help! Eric Van Otterloo

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FastQC Issue with per base nucleotide quality graph
by Tan, Justin 05 Apr '13

05 Apr '13

Hi, I am having a problem with FastQC. When I view per base quality, it gives me a strange looking graph: [cid:2f881b69-b1f8-4c44-8f23-2cf3799bd703@fasmail.harvard.edu] I am wondering it this is because of a problem with my data? None of my colleagues have seen this before. Thanks! Justin

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Uploading Illumina BAM files in Galaxy
by Priya Bhatt 28 Mar '13

28 Mar '13

Dear Galaxy Users, I had a quick question as a new Galaxy user. I just received WGS data from Illumina where I'm given .bam files. I am not given .fastq files. Most of the Galaxy tutorials I've seen online have started with raw data in .fastq format. Can Galaxy handle if I were to upload a .bam file using the "Get Data" tool? Furthermore are there any examples/workflows available that others have created when using Illumina data Thank you! Best, Priya

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downloads MAF files
by Navin Rustagi 28 Mar '13

28 Mar '13

Hi, I want to download MAF files for the exonic regions of the genome. Additionally I want the sequences to be in phase as well. How does one do that. Regards, Navin

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